Immunostimulatory and anti-allergic potential of novel heterotrimeric lectin from seeds of Zizyphus mauritiana Lam.

Zizyphus mauritiana Lam. seeds (ZMS) have been used medicinally as sedative or hypnotic drugs in most of Asian countries. ZMS has significant benefits to the human health. Therefore, we have evaluated immunomodulatory effect of lectin extracted from these ZMSL in both in vitro and in vivo study. Anaphylaxis is a severe life-threatening allergic reaction and Arthus reaction is deposition of immune complex and complement system activation, so we hypothesized that if ZMSL can protect these severe allergic diseases. We have studied the effect of ZMSL on macrophages and Wistar albino rats and confirmed its protective effect against anaphylaxis and Arthus reaction. Results of this study suggest ZMSL have immunostimulatory and antiallergic activity.

Therapeutic potential of root and stem bark of wild medicinal plant Ziziphus mauritiana (Lamk.) against silica induced toxicity in Wistar albino rats.

ETHNOPHARMACOLOGICAL RELEVANCE: Ziziphus mauritiana (Lamk.) of the family Rhamnaceae is a traditional herbal medicinal plant commonly called as 'ber' (Indian jujube). It is traditionally used as food source and helps in treatment of various diseases like malaria, asthma, diarrhoea, typhoid, diabetes, skin diseases and acts as a pain killer. AIM OF THE STUDY: This study aimed to evaluate the ability of extracts of barks of root and stem of Ziziphus mauritiana (ZM) to cure silica induced toxicity in Wistar albino rats. MATERIALS AND METHODS: Experiments carried out on the acute toxicity studies of silica and therapeutic potential of ZM extracts were based on OECD guidelines and from these results lethal dose (LD50) values were determined. Silica was administered by IP injection at 20 mg/kg b.w. for 10 days and roots and stem barks extracts of ZM were fed orally by gavage at 400 mg/kg b.w. for 21 days. Assessments of biochemical, haematological parameters as well as liver histological examination were performed in the study. RESULTS: After 21 days of oral feeding of extracts of root and stem of ZM, it was found to alter the liver histology. Significant decrease in enzymes such as ALT, AST, ALP, LDH and urea, creatinine and uric acid levels in serum were recorded. Treatment with extracts could help enzymatic activity of liver antioxidant enzymes to settle towards normal level and significant decrease in the lipid peroxidation along with increase in the value of GSH in liver, was also observed. In addition, extracts of ZM also helped to reduce the serum NO, IL-6 and TNF-α level. CONCLUSION: Results of this study has suggested that the extracts of root and stem bark of ZM can be used for therapeutic purpose to prevent silica induced toxicity. ZM extracts could be utilised as natural antioxidant and immunotherapeutic medicines to protect silica induced cell damage.

Anti-allergic and anti-inflammatory properties of Zizyphus mauritiana root bark.

An allergy may sometimes be very dangerous and one of the main factors responsible for allergy is the complement system which can lead to a life-threatening reaction called anaphylaxis. Cycloxygenase-1 (COX-1), Cycloxygenase-2 (COX-2) and 5-Lipoxygenase (5-LOX) trigger allergic and inflammatory reactions. A number of anti-allergic synthetic drugs are available but are costly and show many side effects. Hence, the ancient traditional system of medication mentioned in Ayurveda finds an edge over various synthetic drugs. Zizyphus mauritiana is referred to as the store house of phytochemicals in Ayurveda. The stem and root barks of Zizyphus mauritiana were dried and powdered under controlled conditions. Extractions of the dried powders were performed separately in different solvents in increasing order of their polarity and were tested for their ability to inhibit the complement system. The aqueous extract of the root bark was found to be more effective in inhibiting the complement system. Fractionation of the aqueous extract resulted in the isolation of the Most Active Fraction (MAF) which inhibited the complement system, COX-1, COX-2, and 5-LOX with IC50 values of 0.006 µg ml(-1), 0.065 µg ml(-1), 0.008 µg ml(-1), and 0.083 µg ml(-1), respectively. The MAF was proven to be successful in down-regulating pro-inflammatory mediators like TNF-α, COX-2, and iNOS when tested on a RAW 264.7 cell line. In vivo, the MAF was found to be preventive against anaphylactic shock and the Arthus reaction, when orally administered daily to Wistar rats. Phytochemical analysis of the MAF has indicated that it is rich in tannins. Results indicate that the MAF, a fraction isolated from the aqueous extract of the root bark of Zizyphus mauritiana, has potent anti-allergic and anti-inflammatory properties.