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1.
J Comp Pathol ; 179: 41-44, 2020 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-32958146

RESUMO

Post-traumatic intraocular sarcomas are rarely reported in domestic animals and are most common in cats. An 8-year-old rabbit (Oryctolagus cuniculus) was referred to a veterinary clinic due to ocular discharge, uveitis and protein precipitate in the anterior chamber of the right eye. The eye was enucleated and histopathological examination revealed a poorly demarcated tumour within the ciliary body with invasion to adjacent eye structures. Neoplastic cells formed chaotic cartilage lacunae and were immunopositive for vimentin but immunonegative for pancytokeratin. On this basis, the neoplasm was diagnosed as a chondrosarcoma. To the best of our knowledge, this is the first report of intraocular chondrosarcoma in a rabbit. There was no history of previous ocular trauma but as there was serological evidence of Encephalitozoon cuniculi infection, inflammation could have been a predisposing factor to development of the neoplasm.


Assuntos
Condrossarcoma/veterinária , Neoplasias Oculares/veterinária , Coelhos , Animais , Feminino
2.
J Comp Pathol ; 172: 80-87, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31690420

RESUMO

Oral cavity tumours and tumour-like lesions are common in dogs and cats, and their diagnosis and classification requires histopathological examination. The aim of this study was to analyse retrospectively oral cavity lesions in dogs and cats in order to evaluate the distribution of inflammatory, hyperplastic and neoplastic lesions manifested as tumours. A total of 486 oral cavity tumours and tumour-like lesions (340 canine; 146 feline), diagnosed routinely from 2015 to 2017, were included. The lesions were classified as inflammatory, hyperplastic or neoplastic (benign and malignant). Histopathological diagnosis was based on haematoxylin and eosin staining and, when necessary, May-Grünwald-Giemsa (for mast cell tumours) or Masson's Fontana (for melanomas) stains or immunohistochemistry (for CD3, CD79α and S100 markers). For dogs, 29.11% (99/340) of the lesions were benign tumours, 24.12% (82/340) were hyperplastic lesions and 14.7% (50/340) were inflammatory lesions. For cats, 4.79% (7/146) were benign tumours, 15.07% (22/146) were hyperplastic lesions and 57.53% (84/146) were inflammatory lesions. Furthermore, 23.24% (79/340) of canine cases were diagnosed with gingival hyperplasia and 19.12% (65/340) were diagnosed with peripheral odontogenic fibroma, while 43.84% (64/146) of feline cases were diagnosed with chronic lymphoplasmacytic stomatitis. Malignant tumours in dogs and cats constituted 32.06% (109/340) and 21.91% (32/146) of the lesions, respectively, with high-grade melanoma in dogs and squamous cell carcinoma in cats being the most common.


Assuntos
Biomarcadores/metabolismo , Doenças do Gato/diagnóstico , Doenças do Cão/diagnóstico , Neoplasias Bucais/veterinária , Boca/patologia , Animais , Doenças do Gato/patologia , Gatos , Doenças do Cão/patologia , Cães , Hiperplasia/metabolismo , Hiperplasia/patologia , Imuno-Histoquímica , Inflamação/metabolismo , Inflamação/patologia , Neoplasias Bucais/diagnóstico , Neoplasias Bucais/patologia , Estudos Retrospectivos
3.
Placenta ; 74: 36-46, 2018 12 15.
Artigo em Inglês | MEDLINE | ID: mdl-30638631

RESUMO

BACKGROUND: Major histocompatibility protein class I (MHC-I) is believed to be expressed in the horse allantochorion only in limited areas at limited times. However, its expression has only been investigated in early pregnancy with non-quantitative techniques that cannot reliably detect small amounts of protein. OBJECTIVE: To quantify the relative expression of MHC-I in the allantochorion and endometrium during days 90-240 of pregnancy (PREG), parturition with physiological delivery of fetal membranes (PHYS), and parturition with retention of these membranes (FMR). Also, to visualize protein expression and determine whether classical or non-classical MHC-I mRNA is expressed. ANIMALS: Heavy draft horses. SETTING: PREG horses (n = 12) were sampled postmortem at a slaughterhouse. PHYS (n = 6) and FMR (n = 5) horses were sampled at farms in the vicinity of Olsztyn, Poland. METHODS: For relative quantification of MHC-I, western blotting with densitometry was used. To visualize MHC-I, immunohistochemistry was used. For mRNA identification, RT-PCR was performed. RESULTS: Although the quantity of MHC-I was lower during PREG than parturition, it was present in the allantochorion and endometrium during PREG. During parturition, MHC-I expression was upregulated in the allantochorion (PHYS vs. PREG: 2.7-times higher, 95% confidence interval, 1.3- to 5.7-times higher; FMR vs. PREG: 3.2-times higher, 95% confidence interval, 1.5- to 6.7-times higher). At parturition, staining for MHC-I was detected in the microcotyledons. Classical and non-classical MHC-I were expressed in both tissues during PREG, PHYS, and FMR. CONCLUSION: MHC-I protein is present in the horse allantochorion and endometrium for at least the first two-thirds of pregnancy and at parturition.


Assuntos
Membrana Corioalantoide/metabolismo , Endométrio/metabolismo , Antígenos de Histocompatibilidade Classe I/metabolismo , Cavalos/imunologia , Placenta/imunologia , Animais , Feminino , Parto/imunologia , Gravidez , Microglobulina beta-2/metabolismo
4.
Pol J Vet Sci ; 19(2): 345-52, 2016.
Artigo em Inglês | MEDLINE | ID: mdl-27487508

RESUMO

The aim of the study was the evaluation of morphology and immunophenotype of canine (19 cases) and feline (7 cases) extramedullary plasmacytomas. Tumours, located in skin, oral cavity and spleen were surgically excised, fixed and processed for histopathology and immunohistochemistry (CD79α, CD18, proliferating cell nuclear antigen, metallothionein). Histologically, tumours were classified into mature, cleaved, asynchronous, polymorphous blastic, hyalin, or monomorphous blastic type. All evaluated tumours showed cytoplasmic expression of CD79α antigen. The expression of CD18 was observed in canine cutaneous and splenic tumours. In canine tumours expression of metallothionein was low to moderate, while in feline plasmacytomas - absent or low. In canine tumours, the mitotic index and proliferating cell nuclear antigen index were positively correlated with the expression of metallothionein. In feline tumours no correlation between mitotic index, proliferating cell nuclear antigen and metallothionein was found. This is the first study describing expression of metallothionein in canine and feline extramedullary plasmacytoma.


Assuntos
Doenças do Gato/patologia , Doenças do Cão/patologia , Neoplasias Bucais/veterinária , Plasmocitoma/veterinária , Neoplasias Cutâneas/veterinária , Neoplasias Esplênicas/veterinária , Animais , Doenças do Gato/imunologia , Gatos , Doenças do Cão/imunologia , Cães , Feminino , Masculino , Neoplasias Bucais/imunologia , Neoplasias Bucais/patologia , Plasmocitoma/imunologia , Plasmocitoma/patologia , Neoplasias Cutâneas/imunologia , Neoplasias Cutâneas/patologia , Neoplasias Esplênicas/imunologia , Neoplasias Esplênicas/patologia
5.
Pol J Vet Sci ; 18(3): 523-31, 2015.
Artigo em Inglês | MEDLINE | ID: mdl-26618584

RESUMO

The aim of this study was to evaluate the effect of low-energy laser irradiation, coenzyme Q10 and vitamin E supplementation on the apoptosis of macrophages and muscle precursor cells during skeletal muscle regeneration after bupivacaine-induced injury. The experiment was conducted on 75 gilts, divided into 5 experimental groups: I--control, II--low-energy laser irradiation, III--coenzyme Q10, IV--coenzyme Q10 and vitamin E, V--vitamin E. Muscle necrosis was induced by injection of 0.5% bupivacaine hydrochloride. The animals were euthanized on subsequent days after injury. Samples were formalin fixed and processed routinely for histopathology. Apoptosis was detected using the TUNEL method. The obtained results indicate that low-energy laser irradiation has a beneficial effect on macrophages and muscle precursor cell activity during muscle post-injury regeneration and protects these cells against apoptosis. Vitamin E has a slightly lower protective effect, limited mainly to the macrophages. Coenzyme Q10 co-supplemented with vitamin E increases the activity of macrophages and muscle precursor cells, myotube and young muscle formation. Importantly, muscle precursor cells seem to be more sensitive to apoptosis than macrophages in the environment of regenerating damaged muscle.


Assuntos
Apoptose/efeitos dos fármacos , Lasers , Doenças Musculares/veterinária , Doenças dos Suínos/terapia , Ubiquinona/análogos & derivados , Vitamina E/farmacologia , Animais , Antioxidantes/uso terapêutico , Apoptose/efeitos da radiação , Bupivacaína/toxicidade , Relação Dose-Resposta à Radiação , Quimioterapia Combinada , Feminino , Músculo Esquelético/efeitos dos fármacos , Doenças Musculares/induzido quimicamente , Doenças Musculares/tratamento farmacológico , Suínos , Ubiquinona/administração & dosagem , Ubiquinona/farmacologia , Vitamina E/administração & dosagem
6.
Placenta ; 36(10): 1167-77, 2015 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-26297153

RESUMO

INTRODUCTION: In mammals, placenta separation at term may involve degradation of the extracellular matrix by matrix metalloproteinases (MMPs). The activity of MMPs is modulated by TIMPs. We hypothesized that the placentas of mares that deliver fetal membranes physiologically and those that retain fetal membranes (FMR) differ in terms of histology; mRNA expression of MMP-2 and MMP-9; protein expression of MMP-2, MMP-9, and TIMP-2; and the potential activity of both MMPs. METHODS: Placenta biopsies were taken from mares (n = 9; 4 FMR, 5 controls) immediately after foal expulsion. Retention was defined as failure to expel all fetal membranes within 3 h of expulsion. All mares were monitored for time of expulsion. The degree of allantochorial/endometrial adhesion was determined in FMR mares, and biopsies from all mares were histologically examined. mRNA expression, protein immunolocalization, protein amount and potential enzyme activity were determined with RT-PCR, immunohistochemistry, Western Blotting and zymography, respectively. RESULTS: FMR mares had strong to extremely strong allantochorial/endometrial adhesion, and significantly more connective tissue in the allantochorial villi than controls. The range of MMP-2 mRNA expression levels was more than 13 times greater in FMR mares than in controls. Protein content of both MMPs and TIMP-2 differed significantly between groups. The range of potential MMP-2 and MMP-9 activity was larger in FMR mares, and MMP-2 potential activity was 1.4 times higher in controls (P = 0.02). DISCUSSION: These results indicate differences in extracellular matrix remodeling in FMR mares and controls, and suggest dysregulation of MMP expression and activation in FMR mares.


Assuntos
Membranas Extraembrionárias/enzimologia , Metaloproteinase 2 da Matriz/metabolismo , Metaloproteinase 9 da Matriz/metabolismo , Placenta Retida/enzimologia , Inibidor Tecidual de Metaloproteinase-2/metabolismo , Animais , Western Blotting , Estudos de Casos e Controles , Tecido Conjuntivo/patologia , Endométrio/metabolismo , Matriz Extracelular/metabolismo , Membranas Extraembrionárias/patologia , Feminino , Cavalos , Placenta/patologia , Placenta Retida/patologia , Gravidez
7.
J Comp Pathol ; 152(2-3): 177-81, 2015.
Artigo em Inglês | MEDLINE | ID: mdl-25555631

RESUMO

An 8.5-month-old male Labrador retriever presented with a cutaneous mass in the right maxillofacial region and swelling of the gingiva. The dog received antibiotic and anti-inflammatory treatment. After 3 weeks the dog returned, presenting with disseminated cutaneous tumours on the neck, trunk and groin. One of the nodules was resected and a cutaneous round cell tumour was diagnosed on microscopical examination. The dog was humanely destroyed. Necropsy examination revealed disseminated tumours in the skin, internal organs and skeletal muscles. Microscopically, all of the tumours were composed of small round cells, arranged in nests. Immunohistochemically, the neoplastic cells expressed vimentin, desmin, MyoD1, myogenin and smooth muscle actin, but were negative for CD3, CD18, CD79αcy, cytokeratin AE1/AE3, chromogranin A, class II molecules of the major histocompatibility complex, neuron-specific enolase and S100. The average Ki67 index was 89.5%. The final diagnosis was a solid variant of alveolar rhabdomyosarcoma (ARMS). This is the first report of the cutaneous multifocal form of ARMS in veterinary oncology.


Assuntos
Doenças do Cão/patologia , Rabdomiossarcoma Alveolar/veterinária , Neoplasias Cutâneas/veterinária , Envelhecimento , Animais , Biomarcadores Tumorais/análise , Cães , Imuno-Histoquímica , Masculino , Rabdomiossarcoma Alveolar/patologia , Neoplasias Cutâneas/patologia
8.
Pol J Vet Sci ; 17(3): 413-20, 2014.
Artigo em Inglês | MEDLINE | ID: mdl-25286647

RESUMO

A morphometric analysis of tumoral Langerhans cells and activated macrophages was conducted using canine cutaneous tumors (65 cases of canine cutaneous histiocytoma and 7 cases of pyogranuloma). The histiocytic origin of the tumor cells was confirmed using immunohistochemistry. The parameters of the morphometric analysis included cellular and nuclear size and shape and the nuclear:cytoplasmic ratio; the variability of these features was calculated separately for each tumor. The canine cutaneous histiocytoma group was divided into four stages of regression depending on the intensity of the lymphocytic infiltration. Statistical analysis revealed that the anisocytosis, anisokaryosis and cellular pleomorphism of tumoral Langerhans cells increased, while the cellular circularity and nuclear:cytoplasmic ratio decreased with tumor regression. Activated macrophages of the pyogranuloma were significantly larger, and had larger nuclei, than tumoral Langerhans cells. Furthermore, these activated macrophages showed greater anisocytosis and anisokaryosis and a lower nuclear:cytoplasmic ratio than tumoral Langerhans cells in the first stages of tumor regression. These results indicate that tumoral Langerhans cells undergo morphologic changes during the regression of canine cutaneous histiocytoma, reflecting their maturation and differentiation. Morphometry can be a useful method for distinguishing activated macrophages from tumoral Langerhans cells.


Assuntos
Doenças do Cão/patologia , Histiocitoma Fibroso Benigno/veterinária , Animais , Cães , Histiocitoma Fibroso Benigno/patologia
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