Frankincense vinegar-processing improves the absorption of boswellic acids by regulating bile acid metabolism.

BACKGROUND: Boswellic acids in Olibanum (known as frankincense) are potent anti-inflammatory properties in treating ulcerative colitis (UC), but its low bioavailability limited drug development. Evidence accumulated that vinegar processing of frankincense exerts positive effects on improving absorption of compositions. The underlying mechanism is unknown. In recent decades, spectacular growth and multidisciplinary integration of metabolic application were witnessed. The relationship between drug absorption and curative effect has been more or less established. However, it remains a knowledge gap in the field between drug absorption and endocrine metabolism. PURPOSE: To investigate the enhancement mechanism of vinegar processing in the absorption of boswellic acids via the aspect of bile acid metabolism. METHODS: The effects of raw frankincense (RF) and processed frankincense (PF) were compared by the UC model of rats. The plasma concentration of boswellic acids and the hepatic and colonic bile acids contents were quantified by UPLC-TQ-MS. The levels of mRNA and protein associated with bile acid metabolism were also compared. RESULTS: The results showed that PF exhibited re-markable mitigating effects on UC with the elevated plasma level of boswellic acid and upregulated expression of the absorption-related protein multidrug resistance-associated protein 2 (MRP2) and organic anion transporting polypeptide 1B3 (OATP1B3) in the liver and colon. It improved colonic lithocholic acid (LCA), which promoted the expression of bile acid nuclear receptors constitutive androstane receptor (CAR) and pregnane X receptor (PXR), resulting in the upregulation of MRP2 and OATP1B3. CONCLUSION: This paper revealed the mechanisms behind the absorption promotion effects of processing. Bile acids metabolism exhibits potential status in pharmaceutical development. The results shed light on the interdisciplinary collaboration between the metabolism and drug absorption fields.

Degradation of petroleum hydrocarbons by embedding immobilized crude oil degrading bacteria.

In this study, the removal effect of free and immobilized bacteria on crude oil was determined. Sodium alginate and polyvinyl alcohol were used as embedding agent, and ramie was modified as an adsorbent to immobilize free bacteria. The conditions for preparing immobilized pellets were optimized using the response surface method, and the best combination was simulated and obtained by Design-Expert 8.0. The best degradation rate of immobilized bacteria was 75.52%. The degradation by free bacteria and immobilized bacteria showed that the selected microorganisms had a good degradation effect on petroleum hydrocarbons.

Emission characteristics and temporal variation of PAHs and their derivatives from an ocean-going cargo vessel.

Polycyclic aromatic hydrocarbons (PAHs), nitro- (NPAHs) and oxy-derivatives (OPAHs) are of considerable concern due to their toxicity and carcinogenic hazards. Ships are recognized as an important emission source of these compounds. Marine diesel oil (MDO) and heavy fuel oil (HFO) are the two most commonly used fuels. The emission characteristics and toxicities of PM2.5-bound PAHs, NPAHs and OPAHs due to HFO and MDO combustion in atypical ocean-going vessel were investigated. The EF variability of polycyclic aromatic compounds (PACs) varied considerably with the fuel formulation (HFO and MDO) and engine loading (20%-100%). The concentration of ΣPACs was 0.63 mg/kWh for MDO and ranged from 2.14 to 9.80 mg/kWh for HFO. Compared to HFO-20%, the EFs of ΣPAHs, ΣNPAHs and ΣOPAHs from MDO-20% were reduced by 97%, 77% and 73%, respectively. As identified through the coefficient of divergence, the profile of HFO-20% was notably different from those under the other three engine loadings for HFO. In addition, the emissions of ΣPAHs and ΣOPAHs showed a significant correlation with PM2.5, while they were relatively weak for ΣNPAHs. However, the CO and PAC emissions were not highly correlated. Furthermore, the BaPeq-ΣPAHs values were 0.010 mg/g for MDO and ranged from 0.092 mg/g to 0.306 mg/g for HFO, and the reduction ranged from 89% to 97% by substituting MDO for HFO. These data highlight the importance of improving fuel quality in close proximity to port areas and are useful for enhancing relevant databases.

Superhydrophobic/superoleophilic cotton-oil absorbent: preparation and its application in oil/water separation.

A superhydrophobic and superoleophilic oil sorbent was prepared by attaching SiO2 particles onto a cotton fiber surface by a sol-gel method and subsequent octadecyltrichlorosilane modification. The surface formation was confirmed by Fourier transform infrared spectroscopy, scanning electron microscopy, and an observation of the water behavior on the cotton surface. The sorption capacity of the modified cotton in pure oil and in an oil/water mixture, the oil adsorption and the reusability were investigated. Compared with raw cotton, the as-prepared cotton absorbed different oils rapidly up to in excess of 25-75 g g-1 its own weight, and the water adsorption was nearly 0 g g-1. The modified cotton fiber could separate oil/water mixtures efficiently through a flowing system. After 10 cycles, the as-prepared cotton was still highly hydrophobic with a 6-times greater adsorption than raw cotton. By a simple modification, a low-cost, high-adsorption and environmentally friendly modified cotton could be prepared that can be considered a promising alternative to organic synthetic fibers to clean up oil spills.

Isolation, screening, and crude oil degradation characteristics of hydrocarbons-degrading bacteria for treatment of oily wastewater.

The aim of this study was to isolate hydrocarbons-degrading bacteria for treatment of oily wastewater from long-standing petroleum-polluted sediments in Bohai Bay, China. Six hydrocarbons-degrading bacteria were screened and identified as Pseudomonas sp. and Bacillus sp. A new approach using a combination of various bacterial species in petroleum biodegradation was proposed and evaluated for its degradation characteristics. Gas chromatography-flame ionization detection (GC-FID) analysis showed that mixed bacterial agents (YJ01) degraded 80.64% of crude oil and 76.30% of crude oil alkanes, exhibiting good biodegradation effect. Besides, after 14 days of culture, the biodegradation assessment markers, pristane and phytane, showed significant degradation rates of 46.75% and 78.23%, respectively. Kinetic analysis indicated that the degradation trends followed a single first-order kinetics model and the degradation half-life (t1/2) of 15 g/L crude oil was significantly shorter (5.48 days). These results indicated that YJ01 could degrade a wider range of hydrocarbons as well as some recalcitrant hydrocarbon components, and can be applied for bioremediation and treatment of oil-contaminated environment.