The role of transduced bone marrow cells overexpressing BMP-2 in healing critical-sized defects in a mouse femur.

The role that transduced mouse bone marrow stromal cells (mBMSCs) engineered to overexpress human bone morphogenetic protein 2 (BMP-2) play in healing critical-sized skeletal defects is largely unknown. We evaluated the interaction between host osteoprogenitor cells and donor mBMSCs transduced with either a lentiviral (LV) vector-expressing red fluorescent protein (RFP) with or without BMP-2 that were implanted into a critical-sized femoral defect. Radiographs taken at the time of killing were evaluated using a five-point scaled scoring system. Frozen histologic sections were analyzed to assess both the transduced cells' role in bone repair and the local osteoprogenitor response. There was complete radiographic bridging in 94% of group I (LV-RFPch-BMP-2-cmyc) and 100% of group III (recombinant human BMP-2) specimens. Radiographs demonstrated a lack of healing in group II (LV-RFPch). Mouse BMSCs transduced with an LV-RFPch-BMP-2 vector were able to induce host cells to differentiate down an osteoblastic lineage and heal a critical-sized defect. However, the donor cells appeared to be functioning as a delivery vehicle of BMP-2 rather than actually differentiating into osteoblasts capable of participating in bone repair as evidenced by a lack of colocalization of the transduced cells to the sites of skeletal repair where the host progenitor cells were found.

Early perioperative use of polytef suspension for the management of facial paralysis after extirpative skull base surgery.

BACKGROUND: The satisfactory management of facial paralysis after extirpative skull base surgery has been notoriously difficult. To optimize physical and psychological recovery, early perioperative use of polytef (polytetrafluoroethylene [PTFE]) facial suspension has been used in patients with either profound electrophysiological or anatomical disruption of the facial nerve. OBJECTIVE: To review the efficacy of this clinical algorithm. STUDY DESIGN: Retrospective medical record review. SETTING: Tertiary care University Hospital Inc, University of Cincinnati College of Medicine, Cincinnati, Ohio. PATIENTS AND METHODS: Medical records review of 32 patients who underwent lateral skull base surgery with resultant facial paralysis who had facial rehabilitation using polytef suspension. RESULTS: All patients who underwent polytef facial suspension reported improvement in both facial function as well as aesthetics. One patient had a late extrusion of the polytef implant. CONCLUSION: The early peri-extirpative application of this technique provides psychological and physical support to patients with facial paralysis who are recovering from lateral skull base surgery. Arch Facial Plast Surg. 2000;2:243-248

Selective indications for the management of extensive anterior epitympanic cholesteatoma via combined transmastoid/middle fossa approach.

OBJECTIVES/HYPOTHESIS: Cholesteatoma that is present in the anterior epitympanic space may extend medially along the supralabyrinthine route to the geniculate ganglion, labyrinth, and cochlea and medially toward Kawase's triangle and the anterior petrous apex. Superiorly it may erode into the middle fossa. Contemporary microsurgical techniques allow for optimal management of these lesions with minimal morbidity, provided that the irregular and complex osteology of the petrous base is understood. The objective of the study was to review the relevant regional anatomy, pathobiology, and current algorithm used in treatment of this select patient population using a combined transmastoid/middle fossa (TM/MF) approach. METHODS: A retrospective review was performed of all clinical and radiographic data from patients undergoing combined TM/MF management of extensive anterior epitympanic cholesteatoma between July 1984 and June 1998. Data from physical examinations, preoperative imaging studies, and operative findings and other relevant data were tabulated and analyzed for patients undergoing TM/MF management of cholesteatoma. RESULTS: Of 488 patients with cholesteatoma treated by the otological service between 1984 and 1998, 11 patients underwent TM/MF exposure and removal of anterior epitympanic cholesteatoma. Total cholesteatoma removal was accomplished in six patients. In three patients, because of facial nerve involvement, labyrinthine fistulae, or internal carotid artery involvement, open-cavity surgery was performed. In two patients, residual or recurrent cholesteatoma was exteriorized at "second-look" procedures. In this small cohort of patients the majority had extension to the arcuate eminence, geniculate ganglion, or Kawase's triangle or had "blue-lining" of the cochlea or labyrinth. To a lesser degree, the middle ear and mastoid contents were involved. Further facial nerve dysfunction or sensorineural hearing loss was not noted after surgery. CONCLUSIONS: Selective TM/MF removal of cholesteatoma provides an optimal route for removing complex cholesteatoma in patients with intact sensorineural function and medial cholesteatoma extension.

Petroclival meningiomas: predictive parameters for transpetrosal approaches.

OBJECTIVE: To determine parameters that influence the selection of the proper petrosal approach or combined approaches for the excision of petroclival meningiomas. METHODS: We dissected 15 cadaver heads, inspected the petroclival region in 50 dry human skulls, and performed a retrospective analysis of the cases of 35 patients with petroclival meningiomas who underwent surgery via transpetrosal approaches. RESULTS: The petroclival region was divided into three "zones" based on the extent of surgical exposure achieved via the petrosal approaches with microscopic dissection of 15 preserved and silicone-injected cadaveric heads and with the measurements of 50 dry skulls. Zone I, defined as the area from the dorsum sellae to the internal auditory canal, is accessible via the anterior petrosal approach. Zone II, defined as the area from the internal auditory canal to the upper border of the jugular tubercle, is easily accessible in its lateral portion via the posterior petrosal approach. The medial portion of Zone II, the "central clival depression," is accessible only with cochlear resection and posterior facial nerve transposition. Zone III, defined as the area from the upper border of the jugular tubercle to the lower edge of the foramen magnum, is accessible via a suboccipital/transcondylar approach. The retrospective analysis of the cases of 35 patients who underwent transpetrosal resection of petroclival meningiomas between 1991 and 1998 was used to determine the predictive value of these anatomic parameters. The degree of tumor resection was analyzed with a novel grading scale combining the percentage of resection and the percentage of brainstem reexpansion. Total excision was achieved in 37% of the patients and complete brainstem reexpansion was achieved in an additional 40%. Residual tumor was concentrated in the central clival depression in Zone II, as predicted by anatomic parameters, and around infiltrated neurovascular structures. New cranial nerve deficit occurred in 31% of the patients in the early postoperative period and improved to 17% at 6 months. Major morbidity occurred in 9% of the patients, and mortality was 0%. Early Karnofsky scores were reduced in 37% of the patients, but 6-month Karnofsky scores were equal to preoperative baseline scores or improved in 91%. CONCLUSION: Anatomic parameters can predict the resectability of petroclival meningiomas. Judicious application of cytoreductive surgery in selected patients maintains an acceptable morbidity and achieves adequate brainstem reexpansion.

Role of aerating mastoidectomy in noncholesteatomatous chronic otitis media.

OBJECTIVE: To assess the success rate of revision tympanoplasty with aerating mastoidectomy in patients with noncholesteatomatous chronic otitis media who had failed at least one prior tympanoplasty. STUDY DESIGN: Retrospective chart review. METHODS: Data were analyzed from 135 patients available for clinical and audiometric studies with a minimum of 18 months' follow-up. All patients had failed at least one prior tympanoplasty and presented with: 1) a persistent tympanic membrane perforation with intermittent drainage, or 2) a wet draining ear, unresponsive to systemic antibiotic and topical management. All patients underwent 1.5-mm, high-density, bone window computed tomography (CT) scanning to assess middle ear, epitympanic, and mastoid air cell pneumatization. All patients underwent revision tympanoplasty with aerating mastoidectomy via a postauricular approach. Patient charts were reviewed for information regarding preoperative radiographic findings, mucosal and ossicular findings at the time of surgery, and success or failure of revision tympanomastoidectomy. RESULTS: The tympanic membrane graft take rate for the entire group of 135 patients was 90.4% (13 grafts failed). A majority of the patients were found to have radiographic and intraoperative evidence of middle ear/mastoid disease. CONCLUSION: For patients with noncholesteatomatous chronic otitis media who have failed prior tympanoplastic reconstruction, an aerating mastoidectomy may be indicated and may improve the success rate of the surgery.

Trigeminal schwannomas: the role of the neurotologist in multidisciplinary management.

Trigeminal neuromas are slow-growing benign tumors representing approximately 10% of all intracranial neuromas and less than 0.5% of all intracranial tumors. Historically, excision of these tumors through traditional neurosurgical routes--including the frontotemporal transsylvian, subtemporal-intradural, subtemporal-transtentorial, or suboccipital approaches--has resulted in an unsatisfactorily high rate of recurrence. In this study we compare contemporary skull base/neurotologic approaches with conventional procedures for trigeminal neuroma extirpation.

The role of endolymphatic mastoid shunt surgery in the managed care era.

OBJECTIVE: Ongoing controversy regarding the surgical management of Meniere's disease has prompted us to review the effectiveness of the endolymphatic mastoid shunt procedure in the control of vertigo. In the current managed care environment in which outcome measures, cost effectiveness, and procedural efficacy must be demonstrated, the surgeon can no longer rely on anecdotal or empirical observations regarding the effectiveness of a treatment paradigm. STUDY DESIGN: Retrooperative case review. SETTING: A tertiary care center. PATIENTS: The records of 327 patients with presumed Meniere's-related vertigo referred to the University of Cincinnati Medical Center were reviewed. One hundred nine patients underwent endolymphatic mastoid shunt. Our study population consists of 96 of these patients that were available for 5 years follow-up. INTERVENTIONS: Endolymphatic mastoid shunt for the control of medically refractory vertigo. MAIN OUTCOME MEASURE: Control of vertigo. RESULTS: Using the Arenberg anatomic classification system, patients with a type I endolymphatic sac achieved 68% control of vertigo, those with a type II endolymphatic sac had a 92% control rate, and patients with a type III endolymphatic sac achieved 78% relief. CONCLUSIONS: Based on an assessment of outcome variables, we conclude that there remains a definite role for endolymphatic shunt surgery in the contemporary approach to patients with Meniere's disease.

Fallopian bridge technique in surgery for chronic ear disease.

Disease that lies in the posterior mesotympanum, including inflammatory polyps, cholesterol granuloma, and cholesteatoma, is often difficult to extirpate. The literature reflects a divided and often controversial opinion regarding the removal of the bony posterior canal wall to reach this disease. Recently, endoscopic visualization has been advocated to enhance exposure. Employing a fallopian bridge technique wherein the bone medial to the facial nerve is opened into the posterior mesotympanum the authors have used this approach in selective circumstances to optimize the eradication of disease. Three hundred patients undergoing tympanomastoidectomy were included in this analysis. The fallopian bridge technique was attempted in 58 cases and was successfully employed in 42 patients. Indications as well as limitations for this procedure are discussed.

Desmoplastic fibroma of the temporal bone.

BACKGROUND: Desmoplastic fibroma is a benign, locally aggressive, intraosseous neoplasm with a propensity for local recurrence. Desmoplastic fibroma most commonly originates within the mandible (70% of cases), and long bones with rare lesions reported in the maxillary, frontal, and parietal bones. We report two patients with desmoplastic fibroma arising within the temporal bone. STUDY DESIGN: Case report and literature review. PATIENTS: Two patients with desmoplastic fibroma with intracranial and extra cranial growth patterns are presented. Both were young females with aural fullness and decreased hearing. INTERVENTIONS: Diagnostic and therapeutic. RESULTS: Both tumors were surgically extirpated and the patients have remained disease free for 18-48 months. Neither tumor expressed estrogen or progesterone receptors. CONCLUSION: Desmoplastic fibroma is a highly invasive local destructive lesion which is best treated by nondestructive surgical intervention. Immunohistochemical analysis may demonstrate hormonal receptors, in which case Tamoxifen may reduce recurrence.

Removal of jugular foramen tumors: the fallopian bridge technique.

Despite recent advances in neuroradiographic and electrophysiologic assessment, the surgical extirpation of lesions of the bony skull base remains challenging. Moreover, as surgeons have gained experience in removing tumors from the irregular osteologic confines of the skull base, attention has been directed toward preservation of vital neural and vascular structures traversing the operative field. This report describes the creation of a fallopian bridge with preservation of the facial nerve in removing tumors that arise within or juxtaposed to the jugular fossa. Thirty-five patients are reported herein with analysis of pathology, surgical approach, and outcome. An algorithm for use of the fallopian bridge, as opposed to facial nerve mobilization and rerouting, is presented with particular emphasis on limitation of this selective procedure.

Temporal bone carcinoma: contemporary perspectives in the skull base surgical era.

Historically, malignant tumors that arose within the temporal bone or that intimately juxtaposed the petrous ridge portended an ominous prognosis. Perusal of the surgical literature from 1950 to 1975 strongly supports the impression that despite heroic surgical efforts many of these patients sustained significant morbidity and a high mortality rate. This report reviews data accumulated over a 10-year period study from July 1984 to June 1994 that examines the changing clinical approach to these lesions from both a diagnostic and therapeutic perspective. Analysis of data from 46 patients cared for at the University of Cincinnati Medical Center is presented with a minimum follow-up of 16 months to a long-term follow-up of 11 years. A management algorithm is presented that reflects our contemporary approach to assessment and management of this select patient population.

Predictability of recovery from Bell's palsy using evoked electromyography.

The role of surgery in the treatment of idiopathic facial paralysis (Bell's Palsy) has been the subject of much controversy. Some have advocated aggressive surgical therapy to prevent nerve injury based on evoked electromyography (EEMG) results. The present study analyzes the outcome of 23 patients who presented with Bell's palsy and were evaluated with EEMG. Of the 15 patients who showed greater than 90 percent compound action potential reduction in the affected side, a widely used criterion for surgical decompression of the facial nerve, almost half (47%) had normal to near-normal recovery, and only three (20%) had residual severe dysfunction. Results infer that patients who meet surgical criteria based on EEMG results but who do not undergo surgery do not show a greater morbidity. The authors conclude that conservative criteria should be used when recommending facial nerve decompression.

Transpetrosal access to meningiomas juxtaposing the temporal bone.

Meningiomas arising from the petroclival dura have presented a challenge to both otolaryngologists and neurosurgeons. Access by means of subtemporal, suboccipital, translabyrinthine, or transcochlear routes have inherent limitations both surgically as well as from resultant morbidity. Since 1988, the authors have used a transpetrosal transtentorial (combined subtemporal suboccipital presigmoid) approach to these lesions in selected cases. The goals of this technique have been to preserve function of the cranial nerves, to avoid excessive retraction of the temporal lobe and cerebellum, and to eliminate damage to the venous sinus and Labbés vein. Exposure of the base of the tumor for ablation of the blood supply and access for tumor removal is an important advantage of this approach.

Surgical aspects of congenital aural atresia.

Surgical repair of congenital aural atresia is a formidable task. The preoperative evaluation, embryology, surgical approaches, postoperative results, complications of surgery, and alternative methods of auditory rehabilitation of these patients are discussed in this article.

Intraoperative facial nerve monitoring in chronic ear surgery: a resident training experience.

Intraoperative facial nerve monitoring has been accepted as a valuable adjunctive modality to be employed in a variety of neurotologic and skull base surgical procedures. However, the role that electrical or mechanical stimulation assessment plays in chronic ear surgery is less well defined, especially in a residency training program. In this study, 250 consecutive operative cases of chronic otitis media, with and without cholesteatoma were monitored with the Xomed-Treace Nerve Integrity Monitor, NIM-2 system. Data regarding electrophysiologic monitoring parameters; electromechanical artifact, including system failure; and surgical outcome were analyzed. Although not a substitute for anatomic identification of the facial nerve, intraoperative monitoring provides an additional technique to optimize resident surgical education.

Transpetrosal approach: surgical anatomy and technique.

Transpetrosal operations have been shown to offer distinct advantages over traditional operations in approaching lesions of the petroclival area. Confusion about these approaches exists due to the variety of names given to these procedures and the lack of detailed descriptions needed to perform them. After extensive review of the literature, we have determined that all transpetrosal techniques fall into one of two categories: anterior petrosectomy or posterior petrosectomy. Combining one of these procedures with existing conventional procedures accurately describes all existing transpetrosal operations and eliminates confusion over nomenclature. In addition, through a series of cadaveric dissections and operative experience, we have detailed each of these procedures as a series of steps that will enable the surgeon to understand the unfamiliar anatomy of the temporal bone and to perform these transpetrosal techniques.

Asymptomatic osteomas of the middle ear.

Osteomas of the middle ear are extremely rare lesions. Of the nine cases reported, eight presented with conductive hearing loss. We recently treated two patients whose osteomas, of the promontory and of the posteroinferior tympanic wall, were asymptomatic. We suggest that middle ear osteomas need not be removed when they do not impinge on the sound conduction system.