Assuntos
Antígenos CD/metabolismo , Linfoma de Zona Marginal Tipo Células B/diagnóstico , Linfoma de Zona Marginal Tipo Células B/metabolismo , Células Neoplásicas Circulantes/metabolismo , Baço/metabolismo , Baço/patologia , Antígenos CD/genética , Antígenos de Superfície/genética , Antígenos de Superfície/metabolismo , Humanos , Imunofenotipagem , Linfoma de Zona Marginal Tipo Células B/genética , Transtornos Linfoproliferativos/metabolismoAssuntos
Cromossomos Humanos Par 2 , Cromossomos Humanos Par 8 , Chaperonas Moleculares/genética , Doenças Mieloproliferativas-Mielodisplásicas/genética , Complexo de Proteínas Formadoras de Poros Nucleares/genética , Receptor Tipo 1 de Fator de Crescimento de Fibroblastos/genética , Translocação Genética , Feminino , Humanos , Pessoa de Meia-IdadeRESUMO
A retrospective cytogenetic study of acute myeloid leukemias (AML) and myelodysplastic syndromes (MDS) was conducted by the Groupe Francophone de Cytogénétique Hématologique (GFCH) to evaluate the structural abnormalities of chromosome 5 associated with other chromosomal abnormalities, in particular of chromosome 7, in these pathologies. In all, 110 cases of AML/MDS were recruited based on the presence of chromosome 5 abnormalities under conventional cytogenetics and supplemented by a systematic fluorescence in situ hybridization study of chromosomes 5 and 7. The abnormalities of the long arm of chromosome 5 (5q) were deletions of various sizes and sometimes cryptic. The 5q abnormalities were associated with translocations in 54% of cases and were simple deletions in 46%. In 68% of cases, 5q deletions were associated with chromosome 7 abnormalities, and 90% of these presented a complex karyotype. Of the 110 patients, 28 had a hematopoietic disorder secondary to chemotherapy, radiotherapy, or both. Among 82 patients with de novo AML/MDS, 63 were older than 60 years. Chromosomal abnormalities often associated hypodiploidy and chromosome 5 and 7 abnormalities in complex karyotypes, features resembling those of secondary hemopathies. Systematic investigation of the exposure to mutagens and oncogenes is thus essential to specify the factors potentially involved in MDS/AML with 5q abnormalities.
Assuntos
Aberrações Cromossômicas , Cromossomos Humanos Par 5 , Cromossomos Humanos Par 7 , Leucemia Mieloide/genética , Síndromes Mielodisplásicas/genética , Doença Aguda , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Antineoplásicos/efeitos adversos , Deleção Cromossômica , Feminino , Humanos , Hibridização in Situ Fluorescente , Masculino , Pessoa de Meia-Idade , Neoplasias Induzidas por Radiação , Translocação GenéticaAssuntos
Fusão Gênica Artificial , Cromossomos Humanos Par 11 , Cromossomos Humanos Par 9 , Proteínas de Homeodomínio/genética , Leucemia Mieloide/genética , Complexo de Proteínas Formadoras de Poros Nucleares/genética , Translocação Genética , Doença Aguda , Sequência de Aminoácidos , Sequência de Bases , DNA de Neoplasias , Humanos , Cariotipagem , Leucemia Mieloide/etiologia , Dados de Sequência MolecularAssuntos
Proteínas de Ligação a DNA/genética , Amplificação de Genes/fisiologia , Síndromes Mielodisplásicas/genética , Proteínas Repressoras/genética , Idoso , Crise Blástica , Aberrações Cromossômicas , Análise Citogenética , Humanos , Masculino , Síndromes Mielodisplásicas/etiologia , Síndromes Mielodisplásicas/patologia , Proteínas Proto-Oncogênicas c-ets , Variante 6 da Proteína do Fator de Translocação ETSRESUMO
The orphan homeobox gene HOX11L2 was previously found to be transcriptionally activated as a result of the t(5;14)(q35;q32) translocation in three T-ALL cases. We now tested by RT-PCR Hox11L2 expression in 23 consecutive cases of T-ALL (15 children aged 0.8-14 years, eight adults aged 17-55 years) and as control 13 B-ALL patients from a single institution. Hox11L2 expression was undetectable in all patients with B-ALL, nor in adults with T-ALL. Nine children (60% of the cases), all boys, expressed Hox11L2. Blast cells from most of the latter patients carried surface CD1a, CD10 and not CD34 antigens, in contrast to the other children. FISH, M-FISH and IPM-FISH analysis failed to detect a t(5;14)(q35;q32) in one of them, which suggests a possible distinct genetic mechanism in Hox11L2 expression induction. Hence, Hox11L2 expression seems to be the most frequent abnormality in childhood T-ALL to date, comparable to the t(12;21) in child B-ALL.