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1.
Med Oral Patol Oral Cir Bucal ; 26(3): e334-e340, 2021 May 01.
Artigo em Inglês | MEDLINE | ID: mdl-33340085

RESUMO

BACKGROUND: Metallothioneins (MTs) gene polymorphisms have been associated with the ability of free radical scavenging and detoxification of heavy metals leading to cancer development. Our aim was to revisit, in a Brazilian population, single-nucleotide polymorphisms (SNPs) of the MT gene family previously associated with oral squamous cell carcinoma (OSCC). MATERIAL AND METHODS: A case-control investigation with 28 OSCC patients and 45 controls was conducted, using conventional risk factors (tobacco use and alcohol consumption) as covariates. SNPs genotyping for rs8052334 (MT1B), rs964372 (MT1B), and rs1610216 (MT2A) was performed by PCR-RFLP, and SNPs for rs11076161 (MT1A) were analyzed by TaqMan assay. RESULTS: The only SNP associated with increased risk for OSCC was the MT-1A AA genotype (OR = 4.7; p = 0.01). We have also evidenced for the first time a significant linkage disequilibrium between the SNPs of MT-2A and MT-1A in this population with the highest frequency (30%) of the unfavorable haplotype G/A/C/T (rs1610216 / rs11076161 / rs964372 / rs8052334) of MT gene polymorphisms (OR = 6.2; p = 0.04). Interestingly, after removing the effects of conventional risk factors, we have uncovered the significance of the AA genotype of the rs11076161 with increased odds of 19-fold higher towards OSCC development. CONCLUSIONS: This is the first demonstration that a significant linkage disequilibrium among gene polymorphisms of the MT family may affect susceptibility to oral cancer, which is conditioned by the G/A/C/T haplotype (rs1610216/rs11076161/rs964372/ rs8052334) and the MT-1A gene polymorphism has a potential clinical utility for the OSCC risk assessment.


Assuntos
Carcinoma de Células Escamosas , Neoplasias de Cabeça e Pescoço , Neoplasias Bucais , Brasil , Carcinoma de Células Escamosas/genética , Estudos de Casos e Controles , Predisposição Genética para Doença , Genótipo , Humanos , Metalotioneína/genética , Neoplasias Bucais/genética , Polimorfismo de Nucleotídeo Único , Fatores de Risco , Carcinoma de Células Escamosas de Cabeça e Pescoço
2.
J Mol Neurosci ; 62(3-4): 276-280, 2017 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-28578517

RESUMO

Primary familial brain calcification (PFBC), formerly known as Fahr disease, is a rare neurological disorder characterized by extensive calcification deposits in the brain. So far, four genes have been reported with variations associated with PFBC, SLC20A2, PDGFß, PDGFRß, and XPR1. Using real-time qPCR, we analyzed the expression of three inorganic phosphate (Pi) transporters (SLC20A1, SLC20A2, and XPR1) in patients with PFBC. Our results showed a significant reduction (~40%) of SLC20A2 expression in the patients carrying mutation whereas no significant change was observed within the patients without known mutations. No difference was detected in SLC20A1 and XPR1 expression between the groups compared to control. The results suggest that mutations in SLC20A2 gene by itself play an import role by reducing its expression in blood of PFBC patients. At the same time, we could not demonstrate a direct co-regulation between the three Pi transporters at mRNA level, once their expression did not change among the groups.


Assuntos
Encefalopatias/genética , Calcinose/genética , Proteínas Cotransportadoras de Sódio-Fosfato Tipo III/genética , Adulto , Idoso , Encefalopatias/patologia , Calcinose/patologia , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Mutação , Linhagem , Receptores Acoplados a Proteínas G/genética , Receptores Acoplados a Proteínas G/metabolismo , Receptores Virais/genética , Receptores Virais/metabolismo , Proteínas Cotransportadoras de Sódio-Fosfato Tipo III/sangue , Proteínas Cotransportadoras de Sódio-Fosfato Tipo III/metabolismo , Receptor do Retrovírus Politrópico e Xenotrópico
3.
Transplant Proc ; 49(1): 169-171, 2017.
Artigo em Inglês | MEDLINE | ID: mdl-28104128

RESUMO

The combined liver-kidney transplantation (cLKT) is the procedure of choice for patients with end-stage liver and kidney disease. In cLKT we can usually accommodate the grafts in two different ways, varying the kidney placement. The retroperitoneal kidney implant has some advantages, such as the easy access or avoiding vascular complications. We propose a new single incision, not yet reported, maintaining an extraperitoneal kidney, with excellent surgical field for cLKT and possible reduction of the impact of wound complications.


Assuntos
Carcinoma Hepatocelular/cirurgia , Falência Renal Crônica/cirurgia , Transplante de Rim/métodos , Cirrose Hepática/cirurgia , Neoplasias Hepáticas/cirurgia , Transplante de Fígado/métodos , Carcinoma Hepatocelular/complicações , Hepatite C Crônica/complicações , Humanos , Falência Renal Crônica/complicações , Cirrose Hepática/complicações , Neoplasias Hepáticas/complicações , Masculino , Pessoa de Meia-Idade , Espaço Retroperitoneal/cirurgia , Resultado do Tratamento
4.
J Comp Pathol ; 153(4): 206-11, 2015 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-26604083

RESUMO

Sheep-associated malignant catarrhal fever (SA-MCF) is an important infectious disease of ruminants worldwide that is caused by ovine herpesvirus 2 (OvHV-2). OvHV-2 is transmitted predominantly by contact between infected and susceptible hosts, while the documentation of vertical transmission is rare. This report presents the pathological and molecular findings associated with transplacental transmission of OvHV-2 in cattle. Two Girolanda cows with corneal oedema, lethargy, mucopurulent nasal discharge and ulcerative stomatitis died spontaneously; one of these was pregnant with a 4-month-old fetus. Significant pathological findings included widespread lymphoplasmacytic necrotizing vasculitis and lymphoplasmacytic accumulations in several organs of both cows and the fetus. A polymerase chain reaction that targeted the tegument protein gene of OvHV-2 amplified viral DNA from the brain of the pregnant cow and her fetus, as well as from the kidney of the pregnant cow. The pathological findings observed in the cow and her fetus, together with the presence of OvHV-2 DNA in tissues of these animals, are suggestive of transplacental transmission of OvHV-2 in SA-MCF in cattle.


Assuntos
Transmissão Vertical de Doenças Infecciosas , Febre Catarral Maligna/transmissão , Doenças dos Ovinos , Animais , Bovinos , Feminino , Herpesviridae , Reação em Cadeia da Polimerase , Gravidez , Ovinos
6.
J Mol Neurosci ; 54(4): 748-51, 2014 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-24969325

RESUMO

Primary familial brain calcification (PFBC) is identified by mineralization of the basal ganglia and other brain regions in the absence of known causes. The condition is often inherited in an autosomal dominant pattern and can manifest itself clinically with neuropsychiatric symptoms such as Parkinsonism, headaches, psychosis, and mood swings. Mutations in the SLC20A2 gene account for ~40% of inherited cases, and this gene encodes an inorganic phosphate transporter (PiT-2), a transmembrane protein associated with Pi homeostasis. The p.Y386X mutation in SLC20A2 was identified in a patient who presented migraines, brain calcification, and mild but chronic hypovitaminosis D. SLC20A2 c.1158C > G single-nucleotide heterozygous mutation results in a premature stop codon and a putative truncated protein of 385 amino acids. Proband parents do not present the mutation, which is also not present in major public SNP databases, suggesting a de novo sporadic trait. This study describes for the first time a de novo SLC20A2 mutation in a PFBC patient with migraine and mild hypovitaminosis D. This data further reinforces the pathogenic role of SLC20A2 mutations as causal factors in PFBC physiopathology.


Assuntos
Encéfalo/patologia , Calcinose/genética , Mutação , Proteínas Cotransportadoras de Sódio-Fosfato Tipo III/genética , Adulto , Calcinose/diagnóstico , Códon de Terminação , Feminino , Humanos , Masculino
7.
Vet Parasitol ; 190(1-2): 277-80, 2012 Nov 23.
Artigo em Inglês | MEDLINE | ID: mdl-22963714

RESUMO

Platynosomum fastosum is a feline biliary tract trematode that generally causes asymptomatic infections. In the early 1980s in Brazil, P. fastosum was associated with cholangiocarcinomas, but this finding was not confirmed in the various publications on the parasite during the last 30 years. This study aims to report three cases of cholangiocarcinomas in cats associated with the presence of P. fastosum. From 2000 to 2011, in the Veterinary Hospital of the Federal University of Campina Grande in northeast Brazil, 348 cats were necropsied, 11 of which (3.16%) were parasitized by P. fastosum. Three cases that resulted in death were associated with cholangiocarcinomas that were found to be associated with P. fastosum. Histologically, the tumors consisted of acini composed of cells with pleomorphic nuclei, loose chromatin, evident nucleoli and lightly eosinophilic cytoplasm. Metastases were observed in two cases. The first case involved metastases to the lungs, kidneys, ovary and peritoneum, and in the second case, the lymph nodes, kidneys, heart and encephalon were involved. The other 8 cats died from other causes, and the parasite was an incidental finding. In those cases, the histologic lesions were nonsuppurative cholangiohepatitis and periductal fibrosis with P. fastosum present. Six animals also showed pre-neoplastic changes (hyperplasia and dysplasia) of the biliary duct epithelium. The study concluded that, as observed in other human biliary tract trematodes, P. fastosum causes cholangiocarcinomas in the liver of cats.


Assuntos
Neoplasias dos Ductos Biliares/veterinária , Ductos Biliares Intra-Hepáticos/parasitologia , Doenças do Gato/parasitologia , Colangiocarcinoma/veterinária , Hepatopatias Parasitárias/veterinária , Infecções por Trematódeos/veterinária , Animais , Neoplasias dos Ductos Biliares/parasitologia , Neoplasias dos Ductos Biliares/patologia , Ductos Biliares Intra-Hepáticos/patologia , Brasil , Doenças do Gato/patologia , Gatos , Colangiocarcinoma/parasitologia , Colangiocarcinoma/patologia , Evolução Fatal , Feminino , Fígado/parasitologia , Fígado/patologia , Hepatopatias Parasitárias/complicações , Hepatopatias Parasitárias/patologia , Metástase Linfática , Masculino , Metástase Neoplásica , Trematódeos/isolamento & purificação , Infecções por Trematódeos/complicações , Infecções por Trematódeos/patologia
8.
Vet Pathol ; 44(6): 928-31, 2007 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-18039908

RESUMO

Craniofacial anomalies, eye malformations, and permanent flexures of the forelimbs are common malformations seen in ruminants grazing semiarid rangelands of Northeastern Brazil. To investigate the cause of these malformations, we fed 2 suspected plants, Mimosa tenuiflora or Prosopis juliflora, to groups of 4 pregnant goats each. Fresh green M. tenuiflora was collected daily and fed ad libitum to 4 goats in group 1 throughout pregnancy. This treatment group also received a supplemental feed concentrate equivalent to 1% body weight. Four goats in group 2 received a ration with 70% of P. juliflora pods and 30% hay throughout pregnancy. Four control goats were fed supplemental feed concentrate (1% body weight) and hay ad libitum throughout pregnancy. Goats treated with P. juliflora pods and the control goats delivered 9 normal kids. The four goats that were fed M. tenuiflora during pregnancy delivered 4 kids, 3 of which had abnormalities similar to those observed in field cases, including cleft lip, unilateral corneal opacity, ocular bilateral dermoids, buphthalmos with a cloudy brownish appearance of the anterior chamber due to an iridal cyst, and segmental stenosis of the colon. Malformations induced experimentally by M. tenuiflora were similar to those observed in field cases, suggesting that M. tenuiflora is a cause of the field cases observed in the Brazilian semiarid rangelands.


Assuntos
Anormalidades Craniofaciais/veterinária , Oftalmopatias/veterinária , Doenças das Cabras/induzido quimicamente , Enteropatias/veterinária , Mimosa/toxicidade , Escoliose/veterinária , Animais , Brasil , Anormalidades Craniofaciais/induzido quimicamente , Oftalmopatias/induzido quimicamente , Oftalmopatias/congênito , Feminino , Cabras , Enteropatias/induzido quimicamente , Enteropatias/congênito , Intoxicação por Plantas , Gravidez , Escoliose/induzido quimicamente , Escoliose/congênito
9.
J Eur Acad Dermatol Venereol ; 20(1): 69-74, 2006 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-16405612

RESUMO

Paraneoplastic pemphigus (PNP) is an autoimmune blistering disease with poor prognosis when associated with malignant neoplasm. We report the case of a patient with PNP associated with a CD20+ non-Hodgkin follicular lymphoma who was treated with Rituximab plus corticosteroids and short courses of cyclosporin. One and a half years after Rituximab therapy, oral ulcerations had cleared and oral methylprednisolone was slowly tapered down without further recurrences. In the course of the disease, the patient developed sepsis due to Listeria monocytogenes and viral infections by human herpes virus 1 and 3. At the end-stage of the disease she developed a cutaneous infection from Mycobacterium chelonae. The patient died 2 years and 7 months after the onset of PNP. Rituximab may be useful for PNP therapy, but further studies are necessary to confirm this hypothesis.


Assuntos
Anticorpos Monoclonais/uso terapêutico , Antineoplásicos/uso terapêutico , Síndromes Paraneoplásicas/tratamento farmacológico , Pênfigo/tratamento farmacológico , Idoso , Anticorpos Monoclonais Murinos , Evolução Fatal , Feminino , Humanos , Síndromes Paraneoplásicas/patologia , Pênfigo/patologia , Rituximab
10.
J Pharm Pharmacol ; 54(4): 577-82, 2002 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-11999137

RESUMO

Acute ethanol intoxication increased triacylglycerides (TAG) and thiobarbituric acid reactive substances (TBARS) in liver and promoted the liberation of epinephrine. Four non-steroidal anti-inflammatory drugs (NSAIDs)--aspirin, naproxen, nimesulide and piroxicam--prevented this increase in TAG and TBARS. Because fatty acids provided by adipose tissue contribute substantially to elevated hepatic TAG in ethanol-intoxicated rats, it was thought that the NSAIDs might reduce epinephrine-stimulated lipolysis in these rats. Isolated rat adipocytes were activated with epinephrine in the presence or absence of the NSAIDs. The NSAIDs inhibited epinephrine-stimulated lipolysis. These drugs did not modify the binding of dihydroalprenolol (beta-adrenergic agonist) to their receptors in isolated guinea-pig liver membranes. The NSAIDs, at concentrations 3,000-fold lower than that of cAMP, inhibited stimulated lipolysis by this messenger. In conclusion, aspirin, naproxen, nimesulide and piroxicam reduce the release of fatty acids from adipose tissue to the liver by inhibiting the epinephrine-stimulated lipolysis, and this, in part, explains the protective action of these NSAIDs against hepatic signs of acute ethanol intoxication.


Assuntos
Adipócitos/efeitos dos fármacos , Anti-Inflamatórios não Esteroides/farmacologia , Bucladesina/farmacologia , Epinefrina/farmacologia , Lipólise/efeitos dos fármacos , Adipócitos/metabolismo , Agonistas Adrenérgicos beta/farmacologia , Animais , Aspirina/farmacologia , Di-Hidroalprenolol/farmacologia , Relação Dose-Resposta a Droga , Interações Medicamentosas , Etanol/farmacologia , Glicerol/metabolismo , Cobaias , Masculino , Naproxeno/farmacologia , Piroxicam/farmacologia , Ratos , Ratos Wistar , Sulfonamidas/farmacologia , Substâncias Reativas com Ácido Tiobarbitúrico/metabolismo , Triglicerídeos/metabolismo
11.
J Emerg Med ; 15(6): 811-4, 1997.
Artigo em Inglês | MEDLINE | ID: mdl-9404797

RESUMO

A 58-year-old man with advanced AIDS presented to the emergency department complaining of headache and decreased vision bilaterally. On evaluation, he was found to have intraocular pressures of 69 and 65 mm Hg. After topical treatment with miotics and apraclonidine, he was given intravenous acetazolamide (Diamox) and peripheral iridotomy was performed. The pressures did not improve significantly. Secondary acute angle closure glaucoma was diagnosed. Emergency physicians should consider this diagnosis when evaluating AIDS patients with visual complaints.


Assuntos
Síndrome da Imunodeficiência Adquirida/complicações , Glaucoma de Ângulo Fechado/etiologia , Acetazolamida/uso terapêutico , Doença Aguda , Serviços Médicos de Emergência , Glaucoma de Ângulo Fechado/terapia , Humanos , Iris/cirurgia , Terapia a Laser , Masculino , Pessoa de Meia-Idade , Mióticos/uso terapêutico , Pilocarpina/uso terapêutico
12.
Scand J Urol Nephrol ; 31(2): 189-91, 1997 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-9165585

RESUMO

A right-renal angiomyolipoma with tumour thrombus into the inferior vena cava was incidentally detected. This is the ninth published case of its type and the second incidental case. Presence of a thrombus does not imply invasive disease, but the tumour then is often bigger than 6 cm and radical surgery is mandatory.


Assuntos
Angiomiolipoma/diagnóstico por imagem , Neoplasias Renais/diagnóstico por imagem , Células Neoplásicas Circulantes , Tomografia Computadorizada por Raios X , Veia Cava Inferior/diagnóstico por imagem , Angiomiolipoma/patologia , Angiomiolipoma/cirurgia , Humanos , Neoplasias Renais/patologia , Neoplasias Renais/cirurgia , Masculino , Pessoa de Meia-Idade , Nefrectomia , Veia Cava Inferior/patologia , Veia Cava Inferior/cirurgia
13.
Emerg Med Clin North Am ; 11(2): 407-19, 1993 May.
Artigo em Inglês | MEDLINE | ID: mdl-8491113

RESUMO

Oncologic patients in urgent medical crises frequently are seen in the Emergency Department. Hypercalcemia, tumor lysis syndrome, hyperviscosity syndrome, and the syndrome of inappropriate antidiuretic hormone are four metabolic complications that have life-threatening implications. This article reviews these conditions from the perspective of the emergency physician. Clinical presentation, pathophysiology, and management specific to each entity are discussed.


Assuntos
Neoplasias/complicações , Viscosidade Sanguínea , Emergências , Humanos , Hipercalcemia/etiologia , Hipercalcemia/terapia , Síndrome de Secreção Inadequada de HAD/etiologia , Síndrome de Secreção Inadequada de HAD/terapia , Neoplasias/sangue , Síndrome de Lise Tumoral/etiologia , Síndrome de Lise Tumoral/terapia
14.
Artigo em Português | LILACS | ID: lil-4515

RESUMO

Os autores estudaram um grupo de trabalhadores ocupacionalmente expostos ao sulfeto de carbono, verificando a influencia deste composto sobre alguns parametros lipidicos. Foram observadas diferencas estatisticamente significativas nos valores dos lipideos totais, do colesterol total, das fracoes beta e alfa-lipoproteinas e nos niveis dos fosfolipideos da fracao alfa-lipoproteinas


Assuntos
Dissulfeto de Carbono , Doenças Profissionais , Lipídeos , Intoxicação
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