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1.
Nat Prod Res ; 35(6): 1038-1041, 2021 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-31135221

RESUMO

The essential oil (EO) from the leaves of Onychopetalum periquino, obtained by hydrodistillation, was analyzed by gas chromatography coupled with mass spectrometry (GC-MS), and also was investigated for its larvicidal activity against Aedes aegypti larvae. Thirteen compounds, representing 91.31% of the crude oil, were identified. Major compounds were sesquiterpenes, including ß-elemene (53.16%), spathulenol (11.94%) and ß-selinene (9.25%). The EO showed high larvicidal activity with a lethal concentration (LC50) of 63.75 µg/mL and 100% mortality at 200 µg/mL. These results represent the first report about the chemical composition of O. periquino and the first larvicidal evaluation with Onychopetalum species.[Figure: see text].


Assuntos
Annonaceae/química , Óleos Voláteis/química , Óleos Voláteis/farmacologia , Folhas de Planta/química , Aedes/efeitos dos fármacos , Animais , Cromatografia Gasosa-Espectrometria de Massas , Inseticidas/química , Inseticidas/farmacologia , Larva/efeitos dos fármacos , Sesquiterpenos/farmacologia
2.
Rev. bras. farmacogn ; 25(1): 11-15, Jan-Feb/2015. tab, graf
Artigo em Inglês | LILACS | ID: lil-746052

RESUMO

Polycarpol, a recurrent lanostane-type triterpene in Annonaceae family, was confirmed by thin layer chromatography and mass spectrometry analysis in the aerial parts (twigs and trunk barks) of Unonopsis duckei R.E. Fr., U. floribunda Diels, U. rufescens (Baill.) R.E. Fr., U. stipitata Diels, Onychopetalum amazonicum R.E. Fr. and Bocageopsis pleiosperma Maas. Its chemotaxonomic significance was discussed for these three genera, as well for the Annonaceae family. In addition, the antimicrobial activity against several strains of microorganisms was evaluated for the first time for this compound, being observed significant antibacterial activity against Staphylococcus aureus (ATCC 6538), Staphylococcus epidermidis (ATCC 1228) and Escherichia coli (ATCC 10538 and ATCC 10799) with minimal inhibitory concentration values between 25 and 50 μg ml−1.

3.
Basic Clin Pharmacol Toxicol ; 113(5): 300-6, 2013 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-23786320

RESUMO

Eudesmols are naturally occurring sesquiterpenoid alcohols that present cytotoxic effect to cancer cells. Herein, all eudesmol isomers displayed cytotoxicity to different tumour cell lines. α-Eudesmol showed IC50 values ranging from 5.38 ± 1.10 to 10.60 ± 1.33 µg/mL for B16-F10 and K562 cell lines, ß-eudesmol showed IC50 values ranging from 16.51 ± 1.21 to 24.57 ± 2.75 µg/mL for B16-F10 and HepG2 cell lines, and γ-eudesmol showed IC50 values ranging from 8.86 ± 1.27 to 15.15 ± 1.06 µg/mL for B16-F10 and K562 cell lines, respectively. In addition, in this work, we studied the mechanisms of cytotoxic action of eudesmol isomers (α-, ß- and γ-eudesmol) in human hepatocellular carcinoma HepG2 cells. After 24-hr incubation, HepG2 cells treated with eudesmol isomers presented typical hallmarks of apoptosis, as observed by morphological analysis in cells stained with haematoxylin-eosin and acridine orange/ethidium bromide. None of eudesmol isomers caused membrane disruption at any concentration tested. Moreover, eudesmol isomers induced loss of mitochondrial membrane potential and an increase in caspase-3 activation in HepG2 cells, suggesting the induction of caspase-mediated apoptotic cell death. In conclusion, the eudesmol isomers herein investigated are able to reduce cell proliferation and to induce tumour cell death by caspase-mediated apoptosis pathways.


Assuntos
Apoptose/efeitos dos fármacos , Sesquiterpenos de Eudesmano/farmacologia , Carcinoma Hepatocelular/metabolismo , Carcinoma Hepatocelular/patologia , Caspase 3/genética , Caspase 3/metabolismo , Linhagem Celular Tumoral , Membrana Celular/efeitos dos fármacos , Proliferação de Células/efeitos dos fármacos , Células Hep G2 , Humanos , Concentração Inibidora 50 , Células K562 , Potencial da Membrana Mitocondrial/efeitos dos fármacos
4.
Planta Med ; 78(14): 1601-6, 2012 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-22814822

RESUMO

Plants are promising sources of new bioactive compounds. The aim of this study was to investigate the cytotoxic potential of nine plants found in Brazil. The species studied were: Annona pickelii Diels (Annonaceae), Annona salzmannii A. DC. (Annonaceae), Guatteria blepharophylla Mart. (Annonaceae), Guatteria hispida (R. E. Fr.) Erkens & Maas (Annonaceae), Hancornia speciosa Gomes (Apocynaceae), Jatropha curcas L. (Euphorbiaceae), Kielmeyera rugosa Choisy (Clusiaceae), Lippia gracilis Schauer (Verbenaceae), and Hyptis calida Mart. Ex Benth (Lamiaceae). Different types of extractions from several parts of plants resulted in 43 extracts. Their cytotoxicity was tested against HCT-8 (colon carcinoma), MDA-MB-435 (melanoma), SF-295 (glioblastoma), and HL-60 (promielocitic leukemia) human tumor cell lines, using the thiazolyl blue test (MTT) assay. The active extracts were those obtained from G. blepharophylla, G. hispida, J. curcas, K. rugosa, and L. gracilis. In addition, seven compounds isolated from the active extracts were tested; among them, ß-pinene found in G. hispida and one coumarin isolated from K. rugora showed weak cytotoxic activity. In summary, this manuscript contributes to the understanding of the potentialities of Brazilian plants as sources of new anticancer drugs.


Assuntos
Compostos Bicíclicos com Pontes/farmacologia , Cumarínicos/farmacologia , Magnoliopsida/química , Monoterpenos/farmacologia , Óleos Voláteis/farmacologia , Extratos Vegetais/farmacologia , Annonaceae/química , Antineoplásicos Fitogênicos , Apocynaceae/química , Monoterpenos Bicíclicos , Brasil , Compostos Bicíclicos com Pontes/química , Compostos Bicíclicos com Pontes/isolamento & purificação , Linhagem Celular Tumoral , Sobrevivência Celular , Clusiaceae/química , Cumarínicos/química , Cumarínicos/isolamento & purificação , Humanos , Hyptis/química , Jatropha/química , Látex/química , Lippia/química , Monoterpenos/química , Monoterpenos/isolamento & purificação , Óleos Voláteis/química , Óleos Voláteis/isolamento & purificação , Componentes Aéreos da Planta/química , Extratos Vegetais/química , Extratos Vegetais/isolamento & purificação , Plantas Medicinais/química
5.
Planta Med ; 78(5): 409-14, 2012 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-22274812

RESUMO

Guatteria friesiana (W. A. Rodrigues) Erkens & Maas (synonym Guatteriopsis friesiana W. A. Rodrigues), popularly known as "envireira", is a medicinal plant found in the Brazilian and Colombian Amazon basin that is used in traditional medicine for various purposes. Recent studies on this species have demonstrated antimicrobial activity. In this study, the antitumor activity of the essential oil from the leaves of G. friesiana (EOGF) and its main components ( α-, ß-, and γ-eudesmol) were determined using experimental models. In the in vitro study, EOGF and its components α-, ß-, and γ-eudesmol displayed cytotoxicity against tumor cell lines, showing IC50 values in the range of 1.7 to 9.4 µg/mL in the HCT-8 and HL-60 cell lines for EOGF, 5.7 to 19.4 µg/mL in the HL-60 and MDA-MB-435 cell lines for α-eudesmol, 24.1 to > 25 µg/mL in the SF-295 and MDA-MB-435 cell lines for ß-eudesmol, and 7.1 to 20.6 µg/mL in the SF-295 and MDA-MB-435 cell lines for γ-eudesmol, respectively. In the in vivo study, the antitumor effect of EOGF was evaluated in mice inoculated with sarcoma 180 tumor cells. Tumor growth inhibition rates were 43.4-54.2 % and 6.6-42.8 % for the EOGF treatment by intraperitoneal (50 and 100 mg/kg/day) and oral (100 and 200 mg/kg/day) administration, respectively. The treatment with EOGF did not significantly affect body mass, macroscopy of the organs, or blood leukocyte counts. Based on these results, we can conclude that EOGF possesses significant antitumor activity and has only low systemic toxicity. These effects could be assigned to its components α-, ß-, and γ-eudesmol.


Assuntos
Antineoplásicos Fitogênicos/administração & dosagem , Guatteria/química , Óleos Voláteis/administração & dosagem , Óleos de Plantas/administração & dosagem , Administração Oral , Animais , Antineoplásicos Fitogênicos/uso terapêutico , Brasil , Linhagem Celular Tumoral , Colômbia , Humanos , Concentração Inibidora 50 , Injeções Intraperitoneais , Masculino , Camundongos , Estrutura Molecular , Óleos Voláteis/uso terapêutico , Folhas de Planta/química , Óleos de Plantas/uso terapêutico , Plantas Medicinais/química , Sarcoma 180 , Sesquiterpenos de Eudesmano/administração & dosagem , Sesquiterpenos de Eudesmano/uso terapêutico
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