Association of OPRM1 rs1799971, HTR1B rs6296 and COMT rs4680 polymorphisms with clinical phenotype among women with fibromyalgia.

To investigate the association between three selected pain polymorphisms and clinical, functional, sensory-related, psychophysical, psychological or cognitive variables in a sample of women with fibromyalgia (FMS). One hundred twenty-three (n = 123) women with FMS completed demographic (age, height, weight), clinical (years with pain, intensity of pain at rest and during daily living activities), functional (quality of life, physical function), sensory-related (sensitization-associated and neuropathic-associated symptoms), psychophysical (pressure pain thresholds), psychological (sleep quality, depressive and anxiety level) and cognitive (pain catastrophizing, kinesiophobia) variables. Those three genotypes of the OPRM1 rs1799971, HTR1B rs6296 and COMT rs4680 single nucleotide polymorphisms were obtained by polymerase chain reactions from no-stimulated whole saliva collection. No significant differences in demographic, clinical, functional, sensory-related, psychophysical, psychological and cognitive variables according to OPRM1 rs1799971, HTR1B rs6296 or COMT rs4680 genotype were identified in our sample of women with FMS. A multilevel analysis did not either reveal any significant gene-to-gene interaction between OPRM1 rs1799971 x HTR1B rs6296, OPRM1 rs1799971 x COMT rs4680 and HTR1B rs6296 x COMT rs4680 for any of the investigated outcomes. This study revealed that three single nucleotide polymorphisms, OPRM1 rs1799971, HTR1B rs6296 or COMT rs4680, mostly associated with chronic pain were not involved in phenotyping features of FMS. Potential gene-to-gene interaction and their association with clinical phenotype in women with FMS should be further investigated in future studies including large sample sizes.

Uso de um jogo de tabuleiro na reabilitação dos membros superiores de idosos institucionalizados em Portugal: um estudo piloto quase-experimental / Board game for the upper limbs rehabilitation in institutionalised elderly from Portugal: a quasiexperimental pilot study

INTRODUÇÃO: A utilização do jogo como estratégia de reabilitação tem revelado um especial impacto nas variáveis cognitivas no idoso, não sendo consensual o seu impacto em indicadores físicos. OBJETIVO: Medir o efeito do treino com um jogo de tabuleiro na melhoria da coordenação dos membros superiores e na força de preensão palmar em idosos institucionalizados. Foi também caracterizada a percepção dos idosos sobre o jogo como estratégia de reabilitação. MATERIAIS E MÉTODOS: Foi conduzido um estudo quase-experimental, longitudinal, incluindo 10 idosos institucionalizados sem alterações significativas na cognição (6CIT 0-7) e na mobilidade dos membros superiores. Este grupo foi inicialmente avaliado (T0) quanto à Força de preensão manual (FPM) e quanto à coordenação dos membros superiores (Bateria de testes EUROFIT), repetindo a avaliação após 2 semanas de terapias convencionais (T1), tendo sido novamente reavaliados 2 semanas após frequentarem as terapias convencionais mais 2 horas adicionais de um programa com um jogo de tabuleiro (T2). Foi ainda coletada a perceção dos idosos sobre a experiência com o jogo. Foi comparada a evolução entre T0-T1 e T1-T2 usando o teste de Wilcoxon. RESULTADOS: Apenas entre T1 e T2 ocorreram mudanças significativas na EUROFIT (p=0.005) e na FPM para ambos os membros (p=0.005; p= 0.007). Os idosos destacaram uma maior relevância do jogo no trabalho em equipe, no estímulo de raciocínio e de agilidade dos membros superiores. CONCLUSÃO: O jogo de tabuleiro é uma potencial ferramenta para completar a terapia convencional, sendo a experiência considerada muito positiva pelos idosos participantes (ClinicalTrials.gov IDIPL10062019).

INTRODUCTION: Using games as a rehabilitation strategy has significantly impacted cognitive variables in the elderly; however, its impact on physical indicators is not consensual. OBJECTIVE: To measure the effect of a training program with a board game on upper limb coordination and handgrip strength of institutionalized elderly. The elderly's perception of playing a board game as a rehabilitation strategy was also characterised. MATERIALS AND METHODS: A longitudinal quasi-experimental study was carried out, including 10 institutionalized elderly people without significant changes in cognition (6CIT 0-7) and upper limb mobility. This group was initially evaluated (T0) for handgrip strength (HGS) and coordination of the upper limbs (EUROFIT test battery), repeating the assessment after 2 weeks of conventional therapies (T1), and also again 2 weeks after attending conventional therapies plus an additional 2 hours of a board game program (T2). The perception of the elderly about their experience with the game was also collected. The evolution between T0-T1 and T1-T2 was compared using the Wilcoxon test. RESULTS: Only between T1 and T2 were significant changes in EUROFIT (p=0.005) and HGS for both members (p=0.005; p= 0.007). A greater relevance of game-based program for teamwork, stimulating reasoning, and agility of the upper limbs was perceived. CONCLUSION: The board game is a potential tool to complement conventional therapy, and the experience is well perceived by the elderly participants (ClinicalTrials.gov IDIPL10062019).