Effect of serine-type protease of Candida spp. isolated from linear gingival erythema of HIV-positive children: critical factors in the colonization.

BACKGROUND: There are several kinds of oral soft tissue lesions that are common manifestations observed in human immunodeficiency virus (HIV)-infected children; for example, linear gingival erythema (LGE) that is a distinctive fiery red band along the margin of the gingivae. The etiology and pathogenesis of LGE are questionable, but a candidal origin has been suggested. Proteases are key virulence attributes produced by a variety of pathogenic fungi, including Candida. The objective of the present study is to identify the protease production in Candida species including, C. albicans (n=5), C. dubliniensis (n=1) and C. tropicalis (n=1), isolated directly from typical LGE lesions observed in six HIV-positive children, and also to test the effect of a serine protease inhibitor on the interaction of Candida spp. and epithelial cells in vitro. METHODS: The ability of Candida strains to release proteases in the culture supernatant fluids was visualized by gelatin-SDS-PAGE. Gel strips containing 30-fold concentrated supernatant (1.5×10(8) yeasts) were incubated at 37°C for 48 h in 50 mM sodium phosphate buffer, pH 5.5. The concentrated supernatants were also incubated with fibronectin, laminin, immunoglobulin G, bovine serum albumin and human serum albumin. The effect of serine protease inhibitor on the interaction of Candida spp. and epithelial cells (MA 104) was measured after pre-treatment of fungi with the inhibitor (phenylmethylsulphonyl fluoride, PMSF). RESULTS: All the extracellular proteases were completely inhibited by PMSF, identifying these activities as serine-type proteases. Interestingly, a common 62-kDa serine protease was observed in all Candida strains. The culture supernatants, rich in serine protease activities, cleaved several soluble proteinaceous substrates. Additionally, we demonstrated that pre-treatment of C. albicans, C. dubliniensis and C. tropicalis with PMSF diminished the interaction with epithelial cells. CONCLUSIONS: Collectively, our results show that Candida spp. isolated from LGE lesions produced and secreted serine proteases and these enzymes may be involved in the initial colonization events.

Infecção pelo HIV em crianças e suas manifestações bucais: revisão de literatura / Pediatric HIV infection and its oral manifestations: a review

O conhecimento dos profissionais da área de Odontologia sobre a infecção pelo HIV e suas manifestações bucais, bem como a realização de uma correta anamnese e exame clínico são fatores muito importantes para o diagnóstico e tratamento precoce de crianças infectadas pelo HIV. O objetivo deste artigo é veicular as informações que irão contribuir para o enriquecimento teórico de clínicos e odontopediatras acerca deste assunto

Infecção pelo HIV em crianças e suas manifestações bucais: revisão de literatura / Pediatric HIV infection and its oral manifestations: a literature review

O conhecimento dos profissionais da área de Odontologia sobre a infecção pelo HIV e suas manifestações bucais, bem como a realização de uma correta anamnese e exame clínico, são fatores muito importantes para o diagnóstico e tratamento precoce de crianças infectadas pelo HIV. O objetivo deste artigo é veicular informações que irão contribuir para o enriquecimento teórico de clínicos e odontopediatras acerca do assunto