RESUMO
OBJECTIVE: We aimed to detect the role and function of microRNA-431 (miR-431) in lung cancer, and to investigate the underlying mechanism in regulating the development of lung cancer. PATIENTS AND METHODS: Quantitative Real-time polymerase chain reaction (qRT-PCR) was utilized to measure the relative expression level of miR-431 in lung cancer tissues and cell lines. Cell counting kit-8 (CCK-8) and colony formation assays were employed to measure the proliferative ability of lung cancer cells. Meanwhile, transwell assay was recruited to detect the invasive and migratory abilities of lung cancer cells. Furthermore, dual-luciferase reporter gene assay was designed to verify the target gene of miR-431. Western blot assay was used to gauge the protein level of DDX5 (DEAD box polypeptide 5). RESULTS: MiR-431 expression was significantly lower in 122 lung cancer tissue samples or cell lines compared to the adjacent normal tissues or lung bronchial epithelial cell line, respectively. Over-expression of miR-431 significantly inhibited proliferation, invasion and migration of A549 cells. Down-regulation of miR-431 accelerated cell growth and metastasis of H1650 cells. DDX5 was proved to be a direct target for miR-431 in lung cancer. CONCLUSIONS: MiR-431 expression decreased in lung cancer tissues and cells. MiR-431 suppressed proliferation, invasion and migration of lung cancer cells via inhibiting the expression of DDX5. Our study might provide a novel target for the biological therapy of lung cancer.
Assuntos
RNA Helicases DEAD-box/genética , Regulação Neoplásica da Expressão Gênica , Neoplasias Pulmonares/genética , MicroRNAs/metabolismo , Células A549 , Movimento Celular/genética , Proliferação de Células/genética , Regulação para Baixo , Técnicas de Silenciamento de Genes , Humanos , Pulmão/patologia , Pulmão/cirurgia , Neoplasias Pulmonares/patologia , Neoplasias Pulmonares/cirurgia , MicroRNAs/genética , Invasividade Neoplásica/genética , PneumonectomiaRESUMO
Objective: To explore the value of ultrahigh b-value DWI in diagnosis of prostate cancer. Methods: From October 2015 to October 2016, a total of 84 cases from Affiliated Changshu Hospital of Soochow University(39 cases of prostate cancer with a total of 57 lesions, 45 cases of benign prostate hyperplasia) were examined with T(2)WI, high b-value DWI (b=1 000 s/mm(2)) and ultrahigh b-value DWI (b=2 000 s/mm(2)) .Three image sets were rated respectively based on PI-RADS V2 by two radiologists and the scores were compared with biopsy results.The differences of the area under the ROC curve (AUC) among the three groups of each observer were compared by Z test. Results: The difference of AUC between ultrahigh b-value DWI and T(2)WI in the diagnosis of peripheral and transitional zone cancer was statistically significant between the two observers (P=0.009 9, 0.008 2, 0.010 8 and 0.004 5 respectively), and there was no significant difference of AUC between ultrahigh b-value DWI and high b-value DWI in the diagnosis of peripheral and transitional zone cancer.The inter-reader agreement was found to be perfect for all lesions, peripheral zone lesions and transition zone lesions at ultrahigh b-value DWI (kappa values were 0.738, 0.709 and 0.768 respectively). Conclusion: The diagnostic performance of ultrahigh b-value DWI is superior to high b-value DWI and T(2)WI in both peripheral zone and transition zone cancers.