Alteración de la calidad de vida en 785 pacientes con rinitis alergica / Imaired quality of life in 785 patients with allergic rhinitis

La incorporación del concepto "Calidad de Vida" ha generado entre los médicos y el personal relacionado con la salud, un cambio de actitud en el cual el único objetivo es el beneficio del paciente. "La calidad de vida relacionada con la salud evalúa no sólo las alteraciones producias por la enfermedad, sino el modo en que el paciente las percibe y los efectos beneficioso o no del tratamiento. Objetivo: describir la frecuencia de las alteraciones de la calidad de vida en pacientes con rinitis.

The object to study was to evaluate quality of life in patients with rhinitis.

Asma severa: incidencia de la admisión en terapia intensiva de pacientes atendidos en el servicio de emergencia de un hospital público / sever asthama: incidence of intensive care unit admission in patients attending a public hospital emergency room

El estado del mal asmatico es definido por la Sociedad Americana de Torax como un ataque de asma grave desde el inicio, o que empeora progresivamente, no respondiendo a las medidas terapeuticas habituales. En las ultimas decadas la prevalencia del asma se ha incrementado como consecuencia de diferentes factores, entre ellos las subestimacion de los sintomas por parte de los pacientes y el subtratamiento medico. El objetivo de este trabajo fue determinar la poblacion de pacientes que consultaron por asma en la guardia del hospital San Roque y que requirieron internacion en Unidad de Terapia Intensiva (UTI), valorando factores desencadenantes e indices de mortalidad. El analisis de los resultados demostro que, de 647 pacientes que consultaron desde el 1 de enero del 2003 al 30 de septiembre del 2006, el 3,55 porciento requirieron internacion en UTI. Se reconocieron como factores desencadenantes a las infecciones respiratorias y el subtratamiento, siendo este ultimo primorial debido a que solo el 8,7 porciento recibia tratamiento preventivo. El indice de mortalidad correspondio al 4.35 porciento de los casos.

The Metabolic Syndrome is an independent predictor of cardiovascular disease in Type 2 diabetic subjects. Prospective data from the Verona Diabetes Complications Study.

AIMS: To evaluate the cardiovascular risk associated with the presence of the Metabolic Syndrome in Type 2 diabetic subjects. METHODS: Subjects with the Metabolic Syndrome, defined by WHO criteria, were identified in a large sample of non-insulin-treated Type 2 diabetic patients examined within the Verona Diabetes Complications Study (n = 946). At baseline and after a mean of 4.5 years follow-up, cardiovascular disease (CVD) was assessed by medical history, physical examination, electrocardiogram (ECG) and echo-duplex of carotid and lower limb arteries. Death certificates and medical records of subjects who died during the follow-up were scrutinized in order to identify CVD deaths. In statistical analyses, CVD was considered as an aggregate end-point, including fatal and non-fatal coronary, cerebrovascular and peripheral vascular disease as well as ischaemic ECG abnormalities and vascular lesions at the echo-duplex. RESULTS: The proportion of subjects with the Metabolic Syndrome was very high (92.3%). At the baseline, 31.7% of subjects were coded positive for CVD, which was more prevalent in subjects with the Metabolic Syndrome (32.9 vs. 17.8%, P = 0.005). Among subjects free of CVD at the baseline (n = 559), CVD events during the follow-up were significantly increased in patients with the Metabolic Syndrome as compared with those without it (19.9% vs. 3.9%, P < 0.001). Multiple logistic regression analysis showed that, along with sex, age, smoking and HbA1c, the presence of the Metabolic Syndrome independently predicted prevalent (OR 2.01, P = 0.045) and incident CVD (OR 4.89, P = 0.031). CONCLUSIONS: In Type 2 diabetes, the presence of the Metabolic Syndrome is associated with an almost 5-fold increase in CVD risk.

Determination of benzene at trace levels in air by a novel method based on solid-phase microextraction gas chromatography/mass spectrometry.

A new method for the determination of benzene at trace levels in air is presented. The method consists of the collection of air samples on adsorbent cartridges with simultaneous adsorption of pre-established amounts of D6-labeled internal standard. Desorption from the cartridge is performed by solid-phase microextraction (SPME) with analysis by gas chromatography/mass spectrometry (GC/MS) using an ion trap mass spectrometer. The influence of several parameters (type of SPME fiber, temperature, time, for example) was investigated, and good linearity in the range 10-400 ng of C6D6, with a coefficient of variance (CV) around 3-5%, was obtained. The method was tested by sampling air in a town center in Italy, and a benzene concentration of approximately 50 microg/m(3) was determined. The maximum limit recommended by the European Community is 10 microg/m(3).

Identification and characterization of fenton oxidation products of surfactants by electrospray mass spectrometry and by solid phase microextraction gas chromatography mass spectrometry. 2. Fatty alcohol polyethoxy sulphates

The Fenton reaction for the degradation of surfactants has been investigated and partial degradation products have been identified and characterized by mass spectrometry for the case of fatty alcohol polyethoxy sulphates. The polar water-soluble products were investigated by liquid chromatography/mass spectrometry (LC/MS) with electrospray ionization (ESI), and the volatile products leaving the mixture during the reaction were trapped by means of solid phase microextraction (SPME) and investigated by gas chromatography/mass spectrometry (GC/MS) with electron and chemical ionization. The oxidation leads to the formation of products with hydroxyl and epoxide groups due to insertion of oxygen atoms or with terminal ethoxylic moieties deriving from the loss of the hydrophilic sulphate group. The formation of volatile aldehydes is also observed, corresponding to the fragmentation between hydrocarbon and ethoxylic chains. The extent of mineralization is dependent on peroxide and iron(II) concentrations. Copyright 2000 John Wiley & Sons, Ltd.

Quantitation of oleuropein in virgin olive oil by ionspray mass spectrometry-selected reaction monitoring.

This work describes the unambiguous evaluation of the presence of oleuropein in virgin olive oils by ionspray tandem mass spectrometry (ISI-MS/MS). The oil samples obtained from different cultivars, such as Carolea, Cassanese, Coratina, Dolce di Rossano, Roggianella, and Tonda di Strongoli, grown in different geographical areas of Calabria, Italy, have shown an average content of oleuropein ranging from 1 ppb to 11 ppm. Commercial virgin oil samples, blended in some cases, contain significant amounts of this pharmacologically important antioxidant. The MS/MS methodology was applied in a triple-quadrupole instrument, through continuous scanning of the third analyzer to detect oleuropein in methanol extracts and in selected ion monitoring (SRM) for its quantitative assay.

Identification and characterization of fenton oxidation products of surfactants by ionspray mass spectrometry and solid-phase microextraction gas chromatography mass spectrometry 1. Lauryl sulphate

The Fenton reaction for the degradation of surfactants has been investigated and partial degradation products have been identified and characterized by mass spectrometry for the case of lauryl sulphate. The polar soluble products were investigated by liquid chromatography/mass spectrometry (LC/MS) with electrospray ionization (ESI), and the volatile products leaving the mixture during the reaction were trapped by means of solid-phase microextraction (SPME) and investigated by gas chromatography/mass spectrometry (GC/MS) with electron and chemical ionization. The oxidation leads to the formation of products with hydroxyl and epoxide groups due to insertion of oxygen atoms or to aldehydes derived from the loss of the hydrophilic sulphate group. The extent of mineralization is dependent on peroxide and iron concentrations. Copyright 1999 John Wiley & Sons, Ltd.

A simple method for the extraction of volatile organic compounds contained in air samples from adsorbent materials by solid phase microextraction and their analysis by gas chromatography/mass spectrometry.

The monitoring of air pollution requires simple, rapid and sensitive sampling and analytical techniques with minimal sample manipulation that are usable for routine analyses. In our laboratories we have developed a method for the analysis of air samples collected by adsorbent cartridges based on solid phase microextraction (SPME) coupled to gas chromatography/mass spectrometry. We investigated the influence of some variables (time and temperature) using toluene as analyte (one of the most common air pollutants) and toluene-d(8) on the SPME extraction, and the sensitivity (LOQ) of the method. We then tested the method on an air sample collected in an industrial area and carried out characterisation of the volatile organic compounds present.

Investigation on the iodination reaction of methylene blue by liquid chromatography-mass spectrometry with ionspray ionisation.

Radioactive iodine (131I and 123I) labelled methylene blue is used for the early diagnosis of melanoma metastases. We studied the iodination reaction of methylene blue (using "cold" iodine) in order to characterise the iodination product(s) as far as number and position of iodine atoms introduced on the aromatic ring(s) is concerned. The reaction was carried out under the same experimental conditions used for the radioactive one, that is in a large excess of methylene blue. The ionspray HPLC-MS analysis of the reaction mixture showed that the iodinated methylene blue was present only in a very small amount and the main iodinated product was a demethylated one, coming out from the iodination of an impurity azure B. We also studied the iodination reaction of azure B in order to better explain the reaction pathway. Commercial azure B contains impurities of methylene blue and all the possible demethylated derivatives. HPLC-MS analysis of the reaction mixture allowed a complete characterisation of the iodinated and bis-iodinated products.

Chemical modification of human albumin at cys34 by ethacrynic acid: structural characterisation and binding properties.

Derivatization of the free cys3,4 in human albumin, which is reported to occur under physiological conditions, has been performed in vitro by reaction of the protein with ethacrynic acid. This modification has been investigated by mass spectrometry and circular dichroism. Ethacrynic acid has been proven to bind human albumin either covalently and non-covalently. This post-translational modification does not determine significant changes in the secondary structure of the protein, as shown by the comparable circular dichroism spectra of the native and the modified proteins. Furthermore, the binding properties of the human albumin samples have been investigated by circular dichroism and equilibrium dialysis. The affinity to the higher affinity binding sites does not change either for drugs binding to site I, like phenylbutazone, or to site II, like diazepam, while a small but significant increase has been observed for bilirubin, known to bind to site III. Nevertheless significant decreases of the affinity at the lower affinity binding sites of the modified protein were observed for both drugs binding to site I or to site II.

Non-covalent complexes between DNA-binding drugs and double-stranded deoxyoligonucleotides: a study by ionspray mass spectrometry.

The non-covalent complexes between some DNA-binding drugs and duplex oligodeoxynucleotides were studied by ionspray mass spectrometry, with the aim of evaluating the suitability of this technique to screen rapidly a series of drugs exerting their activity through non-covalent binding to specific base sequences of DNA. Two classes of drugs were considered, distamycins (which show affinity for the minor groove of DNA) and anthracyclines (which interact through intercalation between bases). For the former, d(CGCGAATTCGCG)2 was chosen as the model oligodeoxynucleotide. Following optimization of sample preparation and instrumental conditions, the complexes of different distamycins were observed; depending on the ligand considered, 1:1 or 2:1 complexes were formed preferentially. A semi-quantitative evaluation of the relative affinities was made by measuring the ratio of the complexes signals to those of the duplex, and also by competitive binding with equimolar amounts of distamycin. For anthracyclines, the daunorubicin-d(CGATCG)2 complex was chosen as the model for a preliminary mass spectrometric study; however, the signals of the duplex and the complex were very low compared with the monomer signal. Since the complex was known to be stable in solution, this was ascribed to gas-phase instability, probably caused by electrostatic repulsion between negatively charged phosphate groups.

Investigation of mammalian lignan precursors in flax seed: first evidence of secoisolariciresinol diglucoside in two isomeric forms by liquid chromatograph/mass spectrometry.

A strong research effort has been devoted in recent years to the mammalian lignans enterolactone and enterodiol because of their claimed protective effects against breast and colon cancer. Some plant lignans appear to be precursors of these molecules. This paper reports a novel identification procedure for one of these precursors, the secoisolariciresinol diglucoside, in the extracts of defatted flax-seed meal. The procedure is based on high-performance liquid chromatography coupled with either ionspray or continuous-flow fast-atom bombardment mass spectrometry. The application of these techniques allowed the identification in flax seed of two isomeric forms of this precursor for the first time. The isomers are reasonable the two diastereoisomers, due to the two equivalent stereogenic centers present in the molecule.