How do fish consume microplastics? An experimental study on accumulation pattern using Nile tilapia (Oreochromis niloticus).

The aim of this study was to investigate microplastic (MP) exposure by Nile tilapia (Oreochromis niloticus) in laboratory conditions. A total of 150 tilapia fishes were equally distributed randomly in 15 different glass tanks with five experimental conditions. Observed results depicted that the presence of MPs in different organs was mainly accumulated from the fish feed rather than externally added MPs in the culture tanks. It was also revealed that the gastrointestinal tract (GIT) was found to be the most susceptible to MPs accumulation followed by gills and muscles in order. However, muscle contained the least size of MPs followed by GITs and gills. A statistical test showed significant correlations among the average length and weight of fish with MP exposure. A filamentous shape was found to be dominant in both GITs and gills while fragment shape was dominant in muscles. FTIR results revealed a total of 12 different polymers in the fish of which two polymers (polyvinyl alcohol and ethylene vinyl acetate) were not detected in the feed-only tanks. Polypropylene (PP) and polyethylene terephthalate (PET) were found to be dominant polymers in all the experimental GIT, gills, and muscle organs. FESEM results indicated the presence of different textures including cracks, edges, flakes, scratches, grooves, and adhering particles. EDX results exhibited the presence of Na, Si, K, Ni, Cu, Zn, As, and Cd in the analyzed samples that may pose additional health risks. Thus, this study could act as baseline data for laboratory-based studies of aquaculture species in future research.

[Fully Automatic Glioma Segmentation Algorithm of Magnetic Resonance Imaging Based on 3D-UNet With More Global Contextual Feature Extraction: An Improvement on Insufficient Extraction of Global Features]. / 3DGE-UNetç£å ±æŒ¯æˆåƒçš„è„‘èƒ¶è´¨ç˜¤å ¨è‡ªåŠ¨åˆ†å‰²ç®—æ³•ï¼šå¯¹ä¸å åˆ†å ¨å±€ç‰¹å¾æå–çš„æ”¹è¿›.

Objective: The fully automatic segmentation of glioma and its subregions is fundamental for computer-aided clinical diagnosis of tumors. In the segmentation process of brain magnetic resonance imaging (MRI), convolutional neural networks with small convolutional kernels can only capture local features and are ineffective at integrating global features, which narrows the receptive field and leads to insufficient segmentation accuracy. This study aims to use dilated convolution to address the problem of inadequate global feature extraction in 3D-UNet. Methods: 1) Algorithm construction: A 3D-UNet model with three pathways for more global contextual feature extraction, or 3DGE-UNet, was proposed in the paper. By using publicly available datasets from the Brain Tumor Segmentation Challenge (BraTS) of 2019 (335 patient cases), a global contextual feature extraction (GE) module was designed. This module was integrated at the first, second, and third skip connections of the 3D UNet network. The module was utilized to fully extract global features at different scales from the images. The global features thus extracted were then overlaid with the upsampled feature maps to expand the model's receptive field and achieve deep fusion of features at different scales, thereby facilitating end-to-end automatic segmentation of brain tumors. 2) Algorithm validation: The image data were sourced from the BraTs 2019 dataset, which included the preoperative MRI images of 335 patients across four modalities (T1, T1ce, T2, and FLAIR) and a tumor image with annotations made by physicians. The dataset was divided into the training, the validation, and the testing sets at an 8∶1∶1 ratio. Physician-labelled tumor images were used as the gold standard. Then, the algorithm's segmentation performance on the whole tumor (WT), tumor core (TC), and enhancing tumor (ET) was evaluated in the test set using the Dice coefficient (for overall effectiveness evaluation), sensitivity (detection rate of lesion areas), and 95% Hausdorff distance (segmentation accuracy of tumor boundaries). The performance was tested using both the 3D-UNet model without the GE module and the 3DGE-UNet model with the GE module to internally validate the effectiveness of the GE module setup. Additionally, the performance indicators were evaluated using the 3DGE-UNet model, ResUNet, UNet++, nnUNet, and UNETR, and the convergence of these five algorithm models was compared to externally validate the effectiveness of the 3DGE-UNet model. Results: 1) In internal validation, the enhanced 3DGE-UNet model achieved Dice mean values of 91.47%, 87.14%, and 83.35% for segmenting the WT, TC, and ET regions in the test set, respectively, producing the optimal values for comprehensive evaluation. These scores were superior to the corresponding scores of the traditional 3D-UNet model, which were 89.79%, 85.13%, and 80.90%, indicating a significant improvement in segmentation accuracy across all three regions (P<0.05). Compared with the 3D-UNet model, the 3DGE-UNet model demonstrated higher sensitivity for ET (86.46% vs. 80.77%) (P<0.05) , demonstrating better performance in the detection of all the lesion areas. When dealing with lesion areas, the 3DGE-UNet model tended to correctly identify and capture the positive areas in a more comprehensive way, thereby effectively reducing the likelihood of missed diagnoses. The 3DGE-UNet model also exhibited exceptional performance in segmenting the edges of WT, producing a mean 95% Hausdorff distance superior to that of the 3D-UNet model (8.17 mm vs. 13.61 mm, P<0.05). However, its performance for TC (8.73 mm vs. 7.47 mm) and ET (6.21 mm vs. 5.45 mm) was similar to that of the 3D-UNet model. 2) In the external validation, the other four algorithms outperformed the 3DGE-UNet model only in the mean Dice for TC (87.25%), the mean sensitivity for WT (94.59%), the mean sensitivity for TC (86.98%), and the mean 95% Hausdorff distance for ET (5.37 mm). Nonetheless, these differences were not statistically significant (P>0.05). The 3DGE-UNet model demonstrated rapid convergence during the training phase, outpacing the other external models. Conclusion: The 3DGE-UNet model can effectively extract and fuse feature information on different scales, improving the accuracy of brain tumor segmentation.

Bioaccumulation and sources of metal(loid)s in fish species from a subtropical river in Bangladesh: a public health concern.

Toxic metals and freshwater fish's metalloid contamination are significant environmental concerns for overall public health. However, the bioaccumulation and sources of metal(loids) in freshwater fishes from Bangladesh still remain unknown. Thus, the As, Pb, Cd, and Cr concentrations in various freshwater fish species from the Rupsha River basin were measured, including Tenualosa ilisha, Gudusia chapra, Otolithoides pama, Setipinna phasa, Mystus vittatus, Glossogobius giuris, and Pseudeutropius atherinoides. An atomic absorption spectrophotometer was used to determine metal concentrations. The mean concentrations of metal(loids) in the fish muscle (mg/kg) were found to be As (1.53) > Pb (1.25) > Cr (0.51) > Cd (0.39) in summer and As (1.72) > Pb (1.51) > Cr (0.65) > Cd (0.49) in winter. The analyzed fish species had considerably different metal(loid) concentrations with seasonal variation, and the distribution of the metals (loids) was consistent with the normal distribution. The demersal species, M. vittatus, displayed the highest bio-accumulative value over the summer. However, in both seasons, none of the species were bio-accumulative. According to multivariate statistical findings, the research area's potential sources of metal(loid) were anthropogenic activities linked to geogenic processes. Estimated daily intake, target hazard quotient (THQ), and carcinogenic risk (CR) were used to assess the influence of the risk on human health. The consumers' THQs values were < 1, indicating that there were no non-carcinogenic concerns for local consumers. Both categories of customers had CRs that fell below the permissible range of 1E - 6 to 1E - 4, meaning they were not at any increased risk of developing cancer. The children's group was more vulnerable to both carcinogenic and non-carcinogenic hazards. Therefore, the entry of metal(loids) must be regulated, and appropriate laws must be used by policymakers.

Microplastics contamination in fish feeds: Characterization and potential exposure risk assessment for cultivated fish of Bangladesh.

Fish feed is becoming an increasingly vital source of nourishment for farmed fish, which are mainly coming from marine fish and agricultural sources. Anthropogenic particles, such as microplastics, are abundant in both marine fish and agricultural byproducts that are utilized to make fish feed. This study investigated whether fish feed could be a source of microplastic contamination, and revealed that a 20 weeks adult farmed tilapia fish might consume up to 268.45 ± 1.438 microplastic particles via fish feed where finisher type feeds were found to be mostly contributory in this number. The microplastics were initially observed with a stereomicroscope and FESEM-EDS. Polymeric composition of microplastics was determined to be polypropylene (PP), nylon-6 (NY-6), polyethylene terephthalate (PET), polystyrene (PS), polyvinyl alcohol (PVA), polyethylene (PE), high- and low-density polyethylene (HDPE, LDPE), ethylene vinyl acetate (EVA), polycarbonate (PC), poly vinyl acetate (PVAc), poly urethane (PU) and polyvinyl chloride (PVC) by FTIR. Results also revealed that the size of microplastic particles in all fish feed ranged from 14 µm to 4480 µm, with 550 ± 45.45 to 11,600 ± 56.1 microplastic particles/kg of fish feed. The FESEM-EDS data demonstrated to overlook the microplastic surface along with attachment of heavy metals onto that surface such as Pb, Ni, and Co in finisher type feed that could create additional health risks.

Assessment of radiation level and potential risk to public living around major hospitals in central and western Bangladesh.

Human beings are continuously bathed in radiation coming from natural and artificial sources. Although the use of radiation in medical applications is beneficial to patients, it also contributes significantly to the health hazard for radiation workers and the public if radiation-generating equipment and radioactive sources are not handled properly. 96% dose contributed from medical uses of ionizing radiation in the US population among man-made sources as per NCRP Report No. 160. There is no extensive study conducted on the large hospitals in Bangladesh following the In-Situ method. We used a real-time digital portable radiation monitor with Garmin eTrex Global Positioning System at 320 monitoring points for radiation monitoring and positioning around the ten largest hospitals in central & western Bangladesh from September to November 2021. The mean radiation dose rates around Bangabandhu Sheikh Mujib Medical University, Dhaka Medical College Hospital, Evercare Hospital, Khulna Medical College Hospital, Mitford Hospital, National Institute of Cancer Research Hospital, Popular Hospital, Rajshahi Medical College Hospital, Shaheed Suhrawardy Medical College Hospital, and Square Hospitals were measured as 0.145 ± 0.012 µSv/h, 0.135 ± 0.009 µSv/h, 0.148 ± 0.008 µSv/h, 0.139 ± 0.01 µSv/h, 0.133 ± 0.007 µSv/h, 0.153 ± 0.011 µSv/h, 0.144 ± 0.012 µSv/h, 0.137 ± 0.008 µSv/h, 0.145 ± 0.01 µSv/h, and 0.153 ± 0.009 µSv/h, respectively. The mean excess lifetime cancer risk (ELCR) of the public who lives nearby the hospital's boundary was estimated at 1.05 × 10-3, 0.983 × 10-3, 1.071 × 10-3, 1.004 × 10-3, 0.964 × 10-3, 1.084 × 10-3, 1.043 × 10-3,0.996 × 10-3, 1.051 × 10-3 & 1.112 × 10-3 respectively. ELCR in most of the locations around the ten largest hospitals in central & western Bangladesh is higher than the global average value. Radiation monitoring is significant for minimizing the public's radiation risk and keeping hospital environments as radiation-free as possible.

GABA content and an antioxidant profile positively correlated with the anticonvulsive activity of Microcos paniculata in acute seizure mice.

This study evaluated the effects of different parts of M. paniculata (MP) extracts on convulsions and antioxidant activities in mice. Six polyphenolic compounds were identified, where epicatechin and quercetin have been identified in the highest amounts (23.01 and 32.23 mg/100 g of dry MP extract, respectively) in MP leaf and stem extracts, using Ultra Performance Liquid Chromatography. 7-day oral administration of MP at doses of 100, 200, and 400 mg/kg body weight (BW) significantly reduced convulsions and reduced mortality rates compared with seizure inducer groups. Antioxidant potentials were measured by superoxide dismutase (SOD), catalase (CAT), thiobarbituric acid reactive substances (TBARS), and reduced glutathione (GSH) content in whole-brain homogenates. Gamma-aminobutyric acid (GABA) levels significantly increased in leaves and stem-treated groups, suggesting that MP leaves and stems have potent antioxidant properties that can attenuate convulsions by modulating the GABAergic system and antioxidant activities.

Heavy metals in popularly sold branded cigarettes in Bangladesh and associated health hazards from inhalation exposure.

Tobacco products are widely recognized as a major contributor to death. Cigarette smoke contains several toxic chemicals including heavy metals particulate causing high health risks. However, limited information has been available on the health risks associated with the heavy metals in cigarettes commonly sold in the Bangladeshi market. This study evaluated the concentrations and potential health risks posed by ten concerned heavy metals in ten widely consumed cigarette brands in Bangladesh using an atomic absorption spectrometer. The concentration (mg/kg) ranges of heavy metals Pb, Cd, Cr, As, Co, Ni, Mn, Fe, Cu, and Zn vary between 0.46-1.05, 0.55-1.03, 0.80-1.2, 0.22-0.40, 0.46-0.78, 2.59-3.03, 436.8-762.7, 115.8-184.4, 146.6-217.7, and 34.0-42.7, respectively. We assume that the heavy metals content among cigarette brands is varied due to the differences in the source of tobacco they use for cigarette preparation. The carcinogenic risks posed by heavy metals follow the order of Cr > Co > Cd > As > Ni > Pb, while the non-carcinogenic risks for Cu, Zn, Fe, and Mn were greater than unity (HQ > 1), except for Fe. The existence of toxic heavy metals in cigarette tobacco may thus introduce noticeable non-carcinogenic and carcinogenic health impacts accompanying inhalation exposure. This study provides the first comprehensive report so far on heavy metal concentration and associated health risks in branded cigarettes commonly sold in Bangladesh. Hence, this data and the information provided can serve as a baseline as well as a reference for future research and have potential implications for policy and legislation in Bangladesh.

Lipoid Proteinosis: Identification of a Novel Nonsense Mutation c.1246C>T:p.R416X in ECM1 gene from Bangladesh.

Lipoid proteinosis is a rare multisystem genodermatosis inherited as autosomal recessive trait. We report a case of lipoid proteinosis in a 10-year-old boy born to first-degree consanguineous parents presented with marked hoarseness of voice, accelerated photoaging appearance, enlarged and erythematous tongue with restricted movement and widespread dermatoses. Biopsy of oral mucosa revealed Periodic acid-Schiff (PAS)-positive amorphous eosinophilic hyaline deposits. Mutational analysis revealed a homozygous nonsense mutation with C to T substitution at nucleotide position 1246(c.1246C>T) in exon-8 of the extracellular matrix protein 1 gene leading to a stop codon. Both the parents were unaffected heterozygous carriers. To our knowledge, this is the first case report of lipoid proteinosis with evidence of a novel nonsense genetic mutation from Bangladesh.

Occurrence of microplastics in tap and bottled water, and food packaging: A narrative review on current knowledge.

Nowadays, microplastic has been detected in many environmental samples, including aquatic and terrestrial environments. However, few studies recently have addressed their attention to microplastic contamination in different drinking sources and food packages. This review paper has narrated those few findings in brief. Literature showed that different pieces of microplastic fragments, e.g., polypropylene (PP), polystyrene (PS), polyethylene terephthalate (PET), polyvinyl chloride (PVC), polyethylene (PE), high-density polyethylene (HDPE), low-density polyethylene (LDPE), etc. are detected in plastic drinking bottle, tap water, and food packaging containers. Microplastic fragmentation may be associated with mechanical stress, UV radiation, low plastic material quality, aging factor, and atmospheric deposition. Besides these, microplastic is a hub of different chemical compounds and can also retain other complex materials from the surroundings. This makes the microplastic contamination even more complicated and difficult to detect them accurately in a single method. Additionally, one of the common practices at the community level is the long-time repeated usage of plastic drinking bottles and food boxes that subsequently cause microplastic leaching and potential health threats to consumers. This narrative study summarizes the current scenario of microplastic contamination from drinking bottles and food containers and emphasizes doing more quality research in this subtle but highly imposed field to understand potential exposure better.

Exposure and health risks of metals in imported and local brands' lipsticks and eye pencils from Bangladesh.

Elemental contamination in cosmetics is a serious health concern as it can pose a cumulative effect on the user's body over a long period. The prime motive of the study was to assess the concentration of 10 concerning chemical elements (Pb, Cd, Cr, As, Co, Ni, Cu, Zn, Fe, and Mn) in imported and local lipsticks and eye pencil samples collected from retail outlets in central Bangladesh (Dhaka city) and to assess their dynamic health risks for users. A total of 18 lipsticks and 24 eye pencils were studied and concentrations of chemical elements were examined with atomic absorption spectrophotometer. The health risk assessment was performed for dermal and ingestion routes of the contaminants. The results reveal that the concentrations of the examined elements vary with colors, brands, and origins. Pb and As concentrations were found below the permissible limit but Cr concentration in some samples exceeded the allowable limit in cosmetics. Cd was not detected in any samples; however, other examined elements such as Co, Ni, Cu, Mn, Zn, and Fe were detected in considerable concentrations. Elements like Mn, Zn, and Fe were found at high levels. In the case of lipstick samples, elemental concentrations followed the order of Fe > Zn > Mn > Ni > Cr > Cu > Pb > Co > As, while the order was Fe > Cu > Mn > Zn > Ni > Cr > Co > Pb > As for eye pencil samples. Results of the hazards quotient (HQ) indicate that there were no non-carcinogenic or carcinogenic risks of elements in samples for dermal exposure. But the cancer risk values of Cr (HQ > 1 for ingestion) in brown color lipsticks and Ni indicate that lipsticks have some carcinogenic effects if they enter the user's body. Dermal cancer risk for eye pencils has also been calculated and for Pb, Cr, Ni, and As; the values were found within the acceptable ranges of 10-6-10-4. It is suggested that the allowable limit of all toxic elements in cosmetics must be established. Furthermore, continuous monitoring is urgently needed for personal care products like lipsticks and eye pencils commonly available in the local markets in the country like Bangladesh.

Exposure to polystyrene nanoplastics induces an anxiolytic-like effect, changes in antipredator defensive response, and DNA damage in Swiss mice.

Although the in vivo toxicity of nanoplastics (NPs) has already been reported in different model systems, their effects on mammalian behavior are poorly understood. Thus, we aimed to evaluate whether exposure to polystyrene (PS) NPs (diameter: 23.03 ± 0.266 nm) alters the behavior (locomotor, anxiety-like and antipredator) of male Swiss mice, induces brain antioxidant activity, and erythrocyte DNA damage. For this, the animals were exposed to NPs for 20 days at different doses (6.5 ng/kg and 6500 ng/kg). Initially, we did not observe any effect of pollutants on the locomotor activity of the animals (inferred via open field test and Basso mouse scale for locomotion). However, we noticed an anxiolytic-like behavior (in the open field test) and alterations in the antipredatory defensive response of mice exposed to PS NPs, when confronted with their predator potential (snake, Pantherophis guttatus). Furthermore, such changes were associated with suppressing brain antioxidant activity, inferred by lower DPPH radical scavenging activity, reduced total glutathione content, as well as the translocation and accumulation of NPs in the brain of the animals. In addition, we noted that the treatments induced DNA damage, evaluated via a single-cell gel electrophoresis assay (comet assay) applied to circulating erythrocytes of the animals. However, we did not observe a dose-response effect for all biomarkers evaluated and the estimated accumulation of PS NPs in the brain. The values of the integrated biomarker response index and the results of the principal component analysis (PCA) and the hierarchical clustering analysis confirmed the similarity between the responses of animals exposed to different doses of PS NPs. Therefore, our study sheds light on how PS NPs can impact mammals and reinforce the ecotoxicological risk associated with the dispersion of these pollutants in natural environments and their uptake by mammals.

Toxicity assessment of SARS-CoV-2-derived peptides in combination with a mix of pollutants on zebrafish adults: A perspective study of behavioral, biometric, mutagenic, and biochemical toxicity.

The dispersion of SARS-CoV-2 in aquatic environments via the discharge of domestic and hospital sewage has been confirmed in different locations. Thus, we aimed to evaluate the possible impacts of zebrafish (Danio rerio) exposure to SARS-CoV-2 peptide fragments (PSPD-2001, 2002, and 2003) alone and combined with a mix of emerging pollutants. Our data did not reveal the induction of behavioral, biometric, or mutagenic changes. But we noticed an organ-dependent biochemical response. While nitric oxide and malondialdehyde production in the brain, gills, and muscle did not differ between groups, superoxide dismutase activity was reduced in the "PSPD", "Mix", and "Mix+PSPD" groups. An increase in catalase activity and a reduction in DPPH radical scavenging activity were observed in the brains of animals exposed to the treatments. However, the "Mix+PSPD" group had a higher IBRv2 value, with NO levels (brain), the reduction of acetylcholinesterase activity (muscles), and the DPPH radical scavenging activity (brain and muscles), the most discriminant factors for this group. The principal component analysis (PCA) and hierarchical clustering analysis indicated a clear separation of the "Mix+PSPD" group from the others. Thus, we conclude that exposure to viral fragments, associated with the mix of pollutants, induced more significant toxicity in zebrafish adults than in others.

Assessment of heavy metals and radionuclides in groundwater and associated human health risk appraisal in the vicinity of Rooppur nuclear power plant, Bangladesh.

This study was carried out to assess the groundwater quality through estimating trace and heavy metal concentration and radionuclide levels in the vicinity of the Rooppur Nuclear Power Plant (RNPP) sites. Twenty-six (26) parameters, including major cations (K, Na, Mg, Ca) and anions (SO4-2, NO3-), trace and heavy metals (Mn, Fe, Zn, Ni, Co, Pb, Cd, As, Hg, Cu, Li, Be, B, V, Ga, Sr, Ag, Ba) and radionuclides (137Cs, 226Ra, 228Ra, and 40K) were estimated in water samples in the study area. This study revealed that the concentration values (µg/L) of Mn (667.091 ± 7.481), Fe (191.477 ± 3.756), Sr (105.218 ± 13.424), and Zn (23.493 ± 1.134) were the dominant metals in the study area. Different pollution evaluation indices (i.e., HPI, HEI, NI, Cd) data revealed that the study area was under a low to medium level of pollution due to the presence of metals in water. Subsequently, non-carcinogenic and carcinogenic health risks assessments for both adults and children were conducted, which indicated that health risk for the carcinogenic metals were below the threshold level except As through oral exposure for both adult and children. The activity concentrations of 226Ra, 228Ra, and 40K were measured to demonstrate probable radioactivity pollution using Gamma-ray spectrometry (High-resolution HPGe detector). The highest activity concentration of 226Ra, 228Ra, and 40K in groundwater samples were 4.9 ± 1.24 Bq/L (RNPP-15), 1.71 ± 0.43 Bq/L (RNPP-15), and 15.43 ± 3.08 Bq/L (RNPP-15). Among the three studied radionuclides, 40K has the highest average activity concentration. The radiological indicators referred to the annual effective dose (AED) is 0.4273 mSv yr-1, which implies no significant cause of radiological risks and hazards (UNSCEAR guideline value). This study provides a baseline of trace and toxic metal contamination, radioactivity, and radiation levels in the groundwater of the nuclear power plant (being built) area.

Short-term exposure of the mayfly larvae (Cloeon dipterum, Ephemeroptera: Baetidae) to SARS-CoV-2-derived peptides and other emerging pollutants: A new threat for the aquatic environments.

The input of SARS-CoV-2 or its fragments into freshwater ecosystems (via domestic or hospital sewage) has raised concerns about its possible impacts on aquatic organisms. Thus, using mayfly larvae [Cloeon dipterum (L.), Ephemeroptera: Baetidae] as a model system, we aimed to evaluate the possible effects of the combined short exposure of SARS-CoV-2-derived peptides (named PSPD-2001, PSPD-2002, and PSPD-2003 - at 266.2 ng/L) with multiple emerging pollutants at ambient concentrations. After six days of exposure, we observed higher mortality of larvae exposed to SARS-CoV-2-derived peptides (alone or in combination with the pollutant mix) and a lower-body condition index than those unexposed larvae. In the "PSPD" and "Mix+PSPD" groups, the activity of superoxide dismutase, catalase, DPPH radical scavenging activity, and the total thiol levels were also lower than in the "control" group. In addition, we evidenced the induction of nitrosative stress (inferred by increased nitrite production) and reduced acetylcholinesterase activity by SARS-CoV-2-derived peptides. On the other hand, malondialdehyde levels in larvae exposed to treatments were significantly lower than in unexposed larvae. The values of the integrated biomarker response index and the principal component analysis (PCA) results confirmed the similarity between the responses of animals exposed to SARS-CoV-2-derived peptides (alone and in combination with the pollutant mix). Although viral peptides did not intensify the effects of the pollutant mix, our study sheds light on the potential ecotoxicological risk associated with the spread of the new coronavirus in aquatic environments. Therefore, we recommend exploring this topic in other organisms and experimental contexts.

Mechanistic assessment of tolerance to iron deficiency mediated by Trichoderma harzianum in soybean roots.

AIMS: Iron (Fe) deficiency in soil is a continuing problem for soybean (Glycine max L.) production, partly as a result of continuing climate change. This study elucidates how Trichoderma harzianum strain T22 (TH) mitigates growth retardation associated with Fe-deficiency in a highly sensitive soybean cultivar. METHODS AND RESULTS: Soil TH supplementation led to mycelial colonization and the presence of UAOX1 gene in roots that caused substantial improvement in chlorophyll score, photosynthetic efficiency and morphological parameters, indicating a positive influence on soybean health. Although rhizosphere acidification was found to be a common feature of Fe-deficient soybean, the upregulation of Fe-reductase activity (GmFRO2) and total phenol secretion were two of the mechanisms that substantially increased the Fe availability by TH. Heat-killed TH applied to soil caused no improvement in photosynthetic attributes and Fe-reductase activity, confirming the active role of TH in mitigating Fe-deficiency. Consistent increases in tissue Fe content and increased Fe-transporter (GmIRT1, GmNRAMP2a, GmNRAMP2b and GmNRAMP7) mRNA levels in roots following TH supplementation were observed only under Fe-deprivation. Root cell death, electrolyte leakage, superoxide (O2 •- ) and hydrogen peroxide (H2 O2 ) substantially declined due to TH in Fe-deprived plants. Further, the elevation of citrate and malate concentration along with the expression of citrate synthase (GmCs) and malate synthase (GmMs) caused by TH suggest improved chelation of Fe in Fe-deficient plants. Results also suggest that TH has a role in triggering antioxidant defence by increasing the activity of glutathione reductase (GR) along with elevated S-metabolites (glutathione and methionine) to stabilize redox status under Fe-deficiency. CONCLUSIONS: TH increases the availability and mobilization of Fe by inducing Fe-uptake pathways, which appears to help provide resistance to oxidative stress associated with Fe-shortage in soybean. SIGNIFICANCE AND IMPACT OF THE STUDY: These findings indicate that while Fe deficiency does not affect the rate or degree of TH hyphal association in soybean roots, the beneficial effects of TH alone may be Fe deficiency-dependent.

Shedding light on the toxicity of SARS-CoV-2-derived peptide in non-target COVID-19 organisms: A study involving inbred and outbred mice.

Despite advances in research on the vaccine and therapeutic strategies of COVID-19, little attention has been paid to the possible (eco)toxicological impacts of the dispersion of SARS-CoV-2 particles in natural environments. Thus, in this study, we aimed to evaluate the behavioral and biochemical consequences of the short exposure of outbred and inbred mice (male Swiss and C57Bl/6 J mice, respectively) to PSPD-2002 (peptide fragments of the Spike protein of SARS-CoV-2) synthesized in the laboratory. Our data demonstrated that after 24 h of intraperitoneal administration of PSPD-2002 (at 580 µg/kg) the animals did not present alterations in their locomotor, anxiolytic-like, or anxiety-like behavior (in the open field test), nor antidepressant-like or depressive behavior in the forced swimming test. However, the C57Bl/6 J mice exposed to PSPD-2002 showed memory deficit in the novel object recognition task, which was associated with higher production of thiobarbituric acid reactive substances, as well as the increased suppression of acetylcholinesterase brain activity, compared to Swiss mice also exposed to peptide fragments. In Swiss mice the reduction in the activity of superoxide dismutase and catalase in the brain was not associated with increased oxidative stress biomarkers (hydrogen peroxide), suggesting that other antioxidant mechanisms may have been activated by exposure to PSPD-2002 to maintain the animals' brain redox homeostasis. Finally, the results of all biomarkers evaluated were applied into the "Integrated Biomarker Response Index" (IBRv2) and the principal component analysis (PCA), and greater sensitivity of C57Bl/6 J mice to PSPD-2002 was revealed. Therefore, our study provides pioneering evidence of mammalian exposure-induced toxicity (non-target SARS-CoV-2 infection) to PSPD-2002, as well as "sheds light" on the influence of genetic profile on susceptibility/resistance to the effects of viral peptide fragments.

Changes in physiological responses and MTP (metal tolerance protein) transcripts in soybean (Glycine max) exposed to differential iron availability.

Members of MTP (metal tolerance protein) family are potential metal ion transporters, but little is known about how their responses and expression are altered in response to the deficiency and excess of Fe in soybean. In this study, root and shoot length and biomass in addition to leaf chlorophyll score, PSII efficiency and photosynthetic performance index were adversely affected by Fe-deficiency and excess Fe. Fe and S concentrations in the root and shoot, as well as the increased root FCR activity, consistently decreased and increased, respectively, accompanied by elevated Zn levels under Fe deficiency and Fe toxicity. This implies that Fe-uptake of plants subjected to differential Fe availability are likely determined by S and Zn nutritional status. In qPCR analysis, GmMTP5, GmMTP7, GmMTP8, and GmMTP10 genes showed downregulation under Fe shortage, whereas GmMTP6 and GmMTP11 were significantly upregulated due to Fe-toxicity. Further, GmMTP1, GmMTP3, GmMTP6, GmMTP7, and GmMTP10 were significantly induced in response to Fe toxicity, indicating their potential role in metal tolerance. Bioinformatics analysis showed that soybean MTP genes possessed a close relationship with certain Arabidopsis genes (i.e. ZAT, MTPB1) involved in solute transport and metal sequestration. Furthermore, top five motifs of soybean MTP protein correspond to the cation efflux family exhibited strong amino acid and evolutionary similarities with Arabidopsisthaliana. These findings shed light on Fe homeostasis mechanisms in soybean and could be used to regulate Fe uptake through breeding or transgenic manipulations of MTP genes.

Determinants of Hepatitis B Virus (HBV) Infection Among University Students in Central Bangladesh.

This study aimed to determine the seroprevalence and determinants of hepatitis B virus (HBV) infection among university students in Bangladesh. This cross-sectional study was conducted among 614 students from five universities in central Bangladesh. Data were collected on demographic information, immunization history, medical and blood transfusion history through the face-to-face interview. Blood samples were collected and screened for anti-HBsAg using ELISA, HBsAg Rapid Test-cassette, and immune chromatographic test. The overall seroprevalence of HBV infection was 5.0%, and vaccination coverage was 19.2% among the participants. Students having a history of surgery (OR 11.004, 95% CI 3.211-37.707), blood transfusion (OR 5.651, 95% CI 0.965-33.068), being married (OR 4.776, 95% CI 1.508-15.127), and not being vaccinated (OR 9.825, 95% CI 1.130-85.367) were at higher risk of being infected by HBV. This study showed the endemicity of HBV infection among the Bangladeshi population. Marriage, surgical or blood transfusion history, not being vaccinated were the determinants of HBV infection within the study population. Public health initiatives for preventing HBV infection at the university levels should be envisaged.

Environmental arsenic exposure and its contribution to human diseases, toxicity mechanism and management.

Arsenic is a well-recognized environmental contaminant that occurs naturally through geogenic processes in the aquifer. More than 200 million people around the world are potentially exposed to the elevated level of arsenic mostly from Asia and Latin America. Many adverse health effects including skin diseases (i.e., arsenicosis, hyperkeratosis, pigmentation changes), carcinogenesis, and neurological diseases have been reported due to arsenic exposure. In addition, arsenic has recently been shown to contribute to the onset of non-communicable diseases, such as diabetes mellitus and cardiovascular diseases. The mechanisms involved in arsenic-induced diabetes are pancreatic ß-cell dysfunction and death, impaired insulin secretion, insulin resistance and reduced cellular glucose transport. Whereas, the most proposed mechanisms of arsenic-induced hypertension are oxidative stress, disruption of nitric oxide signaling, altered vascular response to neurotransmitters and impaired vascular muscle calcium (Ca2+) signaling, damage of renal, and interference with the renin-angiotensin system (RAS). However, the contributions of arsenic exposure to non-communicable diseases are complex and multifaceted, and little information is available about the molecular mechanisms involved in arsenic-induced non-communicable diseases and also no suitable therapeutic target identified yet. Therefore, in the future, more basic research is necessary to identify the appropriate therapeutic target for the treatment and management of arsenic-induced non-communicable diseases. Several reports demonstrated that a daily balanced diet with proper nutrient supplements (vitamins, micronutrients, natural antioxidants) has shown effective to reduce the damages caused by arsenic exposure. Arsenic detoxication through natural compounds or nutraceuticals is considered a cost-effective treatment/management and researchers should focus on these alternative options. This review paper explores the scenarios of arsenic contamination in groundwater with an emphasis on public health concerns. It also demonstrated arsenic sources, biogeochemistry, toxicity mechanisms with therapeutic targets, arsenic exposure-related human diseases, and onsets of cardiovascular diseases as well as feasible management options for arsenic toxicity.

Amelioration of Metal-Induced Cellular Stress by α-Lipoic Acid and Dihydrolipoic Acid through Antioxidative Effects in PC12 Cells and Caco-2 Cells.

α-Lipoic acid (ALA) and its reduced form dihydrolipoic acid (DHLA) are endogenous dithiol compounds with significant antioxidant properties, both of which have the potential to detoxify cells. In this study, ALA (250 µM) and DHLA (50 µM) were applied to reduce metal (As, Cd, and Pb)-induced toxicity in PC12 and Caco-2 cells as simultaneous exposure. Both significantly decreased Cd (5 µM)-, As (5 µM)-, and Pb (5 µM)-induced cell death. Subsequently, both ALA and DHLA restored cell membrane integrity and intracellular glutathione (GSH) levels, which were affected by metal-induced toxicity. In addition, DHLA protected PC12 cells from metal-induced DNA damage upon co-exposure to metals. Furthermore, ALA and DHLA upregulated the expression of survival-related proteins mTOR (mammalian target of rapamycin), Akt (protein kinase B), and Nrf2 (nuclear factor erythroid 2-related factor 2) in PC12 cells, which were previously downregulated by metal exposure. In contrast, in Caco-2 cells, upon co-exposure to metals and ALA, Nrf2 was upregulated and cleaved PARP-1 (poly (ADP-ribose) polymerase-1) was downregulated. These findings suggest that ALA and DHLA can counterbalance the toxic effects of metals. The protection of ALA or DHLA against metal toxicity may be largely due to an enhancement of antioxidant defense along with reduced glutathione level, which ultimately reduces the cellular oxidative stress.