The investigation of WNT6 and WNT10A single nucleotide polymorphisms as potential biomarkers for dental pulp calcification in orthodontic patients.

The aim of this study is to evaluate if single nucleotide polymorphisms (SNPs) in WNT6 and WNT10A are associated with the risk of dental pulp calcification in orthodontic patients. This cross-sectional study followed the "Strengthening the Reporting of Genetic Association Studies" (STREGA) guidelines. Panoramic radiographs (pre- and post-orthodontic treatment) and genomic DNA from 132 orthodontic patients were studied. Dental pulp calcification (pulp stones and/or pulp space narrowing) was recorded in upper and lower first molars. The SNPs in WNT6 and WNT10A (rs7349332, rs3806557, rs10177996, and rs6754599) were assessed through genotyping analysis using DNA extracted from buccal epithelial cells. The association between pulp calcification and SNPs were analyzed using allelic and genotypic distributions and haplotype frequencies (p<0.05). Prevalence of dental pulp calcification was 42.4% in the 490 studied molars. In the genotypic analysis, the SNPs in WNT10A showed a statistically significant value for molar calcification (p = 0.027 for rs1017799), upper molar calcification (p = 0.040 for rs1017799) (recessive model), and molar calcification (p = 0.046 for rs3806557) (recessive model). In the allelic distribution, the allele C of the SNP rs10177996 in WNT10A was associated with molar calcifications (p = 0.042) and with upper first molar calcification (p = 0.035). Nine combinations of haplotypes showed statistically significant value (p<0.05). The findings of this study indicates that SNPs in WNT10A and WNT6 are associated with dental pulp calcification in molars after orthodontic treatment and may be considered as biomarkers for dental pulp calcification.

Comparação de dois métodos de rugoscopia palatina na identificação humana / Comparison of two methods of palatal rugoscopy in human identification

A Odontologia Legal é cada vez mais requisitada para a obtenção de dados que permitam a identificação humana. Entre muitas ferramentas, a análise das rugas palatinas se destaca como um meio eficaz para a identificação, contudo são necessários estudos que permitam estabelecer padrões de análise que levem a um maior sucesso no processo. Assim, esse estudo teve por objetivo averiguar se há diferença entre a execução da rugoscopia por comparação visual ou por meio de um software. Para isso, foi utilizada uma amostra de 30 indivíduos (n=30), os quais foram submetidos à realização de fotografias intraorais do palato, à obtenção de modelo de gesso do arco alveolar superior com delineamento e ressalte das rugas em cada modelo com grafite, além da tomada de uma fotografia de cada modelo com as rugas demarcadas. Duas análises foram realizadas, sendo a primeira comparação de fotos de modelo de gesso com fotos intraorais via software por sobreposição, e a segunda entre modelos de gesso com fotografias intraorais por análise visual. Com quatro avaliadores em cada análise, registrou-se o resultado obtido de compatibilidade entre modelo-foto, ou foto-foto. Na análise estatística dos dados obtidos, foram aplicados os testes t student (P=0,053) e Kruskal-Wallis (P=0,990), que demonstraram não existir diferença significativa entre os resultados obtidos pelas diferentes análises, e nem variância estatisticamente significativa entre os grupos. Desse modo, pode-se dizer que um software pode ser usado simultaneamente com análise visual

The Forensic Dentistry is being increasingly requested for obtaining data that allows human identification. Among many of its tools, the analysis of the palatal rugae stands out as an effective device for the identification, but more studies are needed to allow an establishment of standards that increases the success rate. So, this study had the main goal to ascertain if there is a difference between choosing the execution of the rugoscopy by visual comparison or by software. This way, it started from a sample of 30 individuals (n=30), in which all of them had their palates photographed, a plaster model from the upper arch with the rugae highlighted with graphite obtained, and finally a photograph of each plaster model made. Two analyses have been done, being the first one a comparison of the photographs of the models and the intraoral ones, on a software by overlapping them, and the second was a comparison between the plaster models and the intraoral photographs, visually. With four evaluators in each analysis, the results from the compatibility among model-photo and photo-photo were registered. In the statistical analysis of this results, the t student t (P=0,053) and the Kruskal-Wallis (P=0,990) tests were performed, and they demonstrated the non-existence of significant difference between the results of the two analysis, nor variance statistically significant between the groups. Being so, it can be said that the software could be simultaneously used with a visual analysis

Use of Immediate Obturator Prosthesis as a Rehabilitation Option in Cases of Squamous Cell Carcinoma.

ABSTRACT: Maxillary defects caused by surgical resection generate abnormal communication between the nasal and oral cavities, hindering proper eating and phonetics, and causing aesthetic damage. Prosthetic intervention is necessary to rehabilitate these patients, in order to block communication and allow correct speech and swallowing functions. This article reports the manufacture of an immediate palatal obturator prosthesis after hemimaxillectomy due to the surgical removal of an intraoral squamous cell carcinoma, which guaranteed better conditions for postoperative recovery, as well as better adaptation to the definitive prosthesis and the use of the prosthetic apparatus.

Endodontical treatment of periapical tooth injury with photodynamic therapy: Case report.

This article describes the use of Photodynamic Therapy (PDT) during the endodontic treatment of teeth with periapical lesion. Patients presented tooth 35 with diagnostic hypotheses of Periapical Cyst or Granuloma. The Crown-Down preparation was performed with the HyFlex CM system. In case I it was not possible to reach the working length, in case II the foraminal debridement was performed at the actual tooth length. In the final irrigation, the E1 - Irrisonic ultrasonic insert was used, promoting sequentially agitation of NaOCl 2.5%, EDTA 17% and NaOCl 2.5%. Then, PDT was applied with 0.005% methylene blue dye. Calcium Hydroxide with Parammonochlorophenol was used and after 15 days, the final irrigation protocol and PDT were performed again. After 90 days of case I and 1 year of case II, the total lesion regression was observed in both cases. It is concluded that the proposed treatment improved the microbial disinfection favoring the regression of the periapical alterations providing satisfactory clinical and radiographic results.