RESUMO
We propose that a beta-turn-beta structure, which plays a critical role in exonucleolytic proofreading in the bacteriophage T4 DNA polymerase, is also present in the Saccharomyces cerevisiae DNA pol delta. Site-directed mutagenesis was used to test this proposal by introducing a mutation into the yeast POL3 gene in the region that encodes the putative beta-turn-beta structure. The mutant DNA pol delta has a serine substitution in place of glycine at position 447. DNA replication fidelity of the G447S-DNA pol delta was determined in vivo by using reversion and forward assays. An antimutator phenotype for frameshift mutations in short homopolymeric tracts was observed for the G447S-DNA pol delta in the absence of postreplication mismatch repair, which was produced by inactivation of the MSH2 gene. Because the G447S substitution reduced frameshift but not base substitution mutagenesis, some aspect of DNA polymerase proofreading appears to contribute to production of frameshifts. Possible roles of DNA polymerase proofreading in frameshift mutagenesis are discussed.
Assuntos
DNA Polimerase III/genética , Mutação da Fase de Leitura , Saccharomyces cerevisiae/genética , Sequência de Aminoácidos , DNA Polimerase III/química , Replicação do DNA , Dados de Sequência Molecular , Mutagênese Sítio-Dirigida , Fenótipo , Saccharomyces cerevisiae/enzimologia , Homologia de Sequência de AminoácidosRESUMO
In vitro selection was used to define sequence contexts that significantly enhanced the mutagenic potential of 7, 8-dihydro-8-oxoguanine (8-oxoG). Contexts that simultaneously reduced the efficiency of 8-oxoG cleavage by formamidopyrimidine DNA N-glycosylase and increased the efficiency of misincorporating A opposite the lesion by DNA polymerase were isolated from a pool of 4(8) random octanucleotide sequences. Kinetic analysis showed that the combined effects of poor repair and high miscoding resulted in 10(2)- to 10(3)-fold increase in the mutagenic potential of 8-oxoG. Furthermore, the isolated sequence contexts correlated strongly with G --> T transversion hotspots in spontaneous mutational spectra reported for the Escherichia coli lacI and human p53 and factor IX genes. We present an example directly linking the interplay between DNA repair and replication to a "high risk sequence" for base substitution.
Assuntos
DNA Polimerase Dirigida por DNA , Proteínas de Escherichia coli , Mutação , Proteínas de Bactérias/genética , Sequência de Bases , Sequência Consenso , DNA , Dano ao DNA , Escherichia coli/genética , Fator IX/genética , Genes p53 , Guanina/análogos & derivados , Guanina/metabolismo , Humanos , Cinética , Repressores Lac , Dados de Sequência Molecular , Proteínas Repressoras/genética , Proteínas Virais/metabolismoRESUMO
Bacteriophage T4 DNA polymerase was inhibited by butylphenyl nucleotides, aphidicolin and pyrophosphate analogs, but with lower sensitivities than other members of the B family DNA polymerases. The nucleotides N2-(p-n-butylphenyl)dGTP (BuPdGTP) and 2-(p-n-butylanilino)dATP (BuAdATP) inhibited T4 DNA polymerase with competitive Ki values of 0.82 and 0.54 microM with respect to dGTP and dATP, respectively. The same compounds were more potent inhibitors in truncated assays lacking the competitor dNTP, displaying apparent Ki values of 0.001 and 0.0016 microM, respectively. BuPdGTP was a substrate for T4 DNA polymerase, and the resulting 3'-BuPdG-primer:template was bound strongly by the enzyme. Each of the non-substrate derivatives, BuPdGDP and BuPdGMPCH2PP, inhibited T4 DNA polymerase with similar potencies in both the truncated and variable competitor assays. These results indicate that BuPdGTP inhibits T4 DNA polymerase by distinct mechanisms depending upon the assay conditions. Reversible competitive inhibition predominates in the presence of dGTP, and incorporation in the absence of dGTP leads to potent inhibition by the modified primer:template. The implications of these findings for the use of these inhibitors in the study of B family DNA polymerases is discussed.
Assuntos
Bacteriófago T4/enzimologia , Inibidores da Síntese de Ácido Nucleico , Nucleotídeos de Adenina/farmacologia , Afidicolina/análogos & derivados , Afidicolina/farmacologia , Sequência de Bases , DNA Polimerase Dirigida por DNA/metabolismo , Nucleotídeos de Desoxiguanina/metabolismo , Nucleotídeos de Desoxiguanina/farmacologia , Nucleotídeos de Guanina/farmacologia , Cinética , Dados de Sequência MolecularRESUMO
Previous studies on the selection of bacteriophage T4 mutator mutants have been extended and a method to regulate the mutator activity of DNA polymerase mutator strains has been developed. The nucleotide changes of 17 bacteriophage T4 DNA polymerase mutations that confer a mutator phenotype and the nucleotide substitutions of several other T4 DNA polymerase mutations have been determined. The most striking observation is that the distribution of DNA polymerase mutator mutations is not random; almost all mutator mutations are located in the N-terminal half of the DNA polymerase. It has been shown that the T4 DNA polymerase shares several regions of homology at the protein sequence level with DNA polymerases of herpes, adeno and pox viruses. From studies of bacteriophage T4 and herpes DNA polymerase mutants, and from analyses of similar protein sequences from several organisms, we conclude that DNA polymerase synthetic activities are located in the C-terminal half of the DNA polymerase and that exonucleolytic activity is located nearer the N terminus.
Assuntos
DNA Polimerase Dirigida por DNA , Mutação , Fagos T/genética , Sequência de Aminoácidos , DNA Viral , Dados de Sequência Molecular , Fenótipo , Homologia de Sequência do Ácido Nucleico , Fagos T/enzimologiaRESUMO
Bacteriophage T4 gene 43 codes for the viral DNA polymerase. We report here the sequence of gene 43 and about 70 nucleotides of 5'- and 3'-flanking sequences, determined by both DNA and RNA sequencing. We have also purified T4 DNA polymerase from T4 infected Escherichia coli and from E. coli containing a gene 43 overexpression vector. A major portion of the deduced amino acid sequence has been verified by peptide mapping and sequencing of the purified DNA polymerase. All these results are consistent with T4 DNA polymerase having 898 amino acids with a calculated Mr = 103,572. Comparison of the primary structure of T4 DNA polymerase with the sequence of other procaryotic and eucaryotic DNA polymerases indicates that T4 DNA polymerase has regions of striking similarity with animal virus DNA polymerases and human DNA polymerase alpha. Surprisingly, T4 DNA polymerase shares only limited similarity with E. coli polymerase I and no detectable similarity with T7 DNA polymerase. Based on the location of specific mutations in T4 DNA polymerase and the conservation of particular sequences in T4 and eucaryotic DNA polymerases, we propose that the NH2-terminal half of T4 DNA polymerase forms a domain that carries out the 3'----5' exonuclease activity whereas the COOH-terminal half of the polypeptide contains the dNTP-binding site and is necessary for DNA synthesis.
Assuntos
Células/enzimologia , DNA Polimerase Dirigida por DNA/genética , Células Eucarióticas/enzimologia , Células Procarióticas/enzimologia , Fagos T/enzimologia , Sequência de Aminoácidos , Sequência de Bases , Evolução Biológica , Cromatografia Líquida de Alta Pressão , Clonagem Molecular , DNA Polimerase Dirigida por DNA/análise , Escherichia coli/enzimologia , Dados de Sequência Molecular , Peso Molecular , PlasmídeosRESUMO
We describe here our first attempt in using suppressor mutations to study structure-function relationships of the bacteriophage T4 DNA polymerase. One intragenic suppressor mutation, J5(43) degrees, was isolated that suppresses the temperature sensitivity but not the mutator activity of tsM19, a DNA polymerase mutant. Thus, the substituted amino acid induced by the tsM19 lesion decreases DNA polymerase fidelity, even if the temperature sensitivity has been corrected by a second amino acid substitution in the DNA polymerase polypeptide. The isolation, mapping and characterization of the J5(43) degrees mutation as well as the purification and characterization of the tsM19-J5(43) degrees mutant DNA polymerase are presented. The suppressor isolation procedure has general applicability for the selection of suppressor mutations of other T4 DNA polymerase mutator mutants.
Assuntos
DNA Polimerase Dirigida por DNA/genética , Supressão Genética , Fagos T/genética , Acriflavina/metabolismo , Troca Genética , DNA Polimerase Dirigida por DNA/metabolismo , Desoxirribonucleases/metabolismo , Resistência Microbiana a Medicamentos , Genes Virais , Mutação , Relação Estrutura-Atividade , Fagos T/enzimologia , TemperaturaRESUMO
The dnaB gene product was purified to homogeneity and its physical properties were characterized. Purification was aided by the use of the Escherichia coli strain. MV12/28, which overproduced the dnaB gene product 10-fold (Wickner, S. H., Wickner, R. B., and Raetz, C. R. H. (1976) Biochem. Biophys. Res. Commun. 70, 389-396) and by taking advantage of the enzyme's high affinity for both DEAE-cellulose and phosphocellulose. The most highly purified fractions gave a single stained band on native, polyacrylamide gels and dnaB enzymatic activity was coincident with this band. On denaturing sodium dodecyl sulfate-polyacrylamide gels, a single band was observed corresponding to a molecular weight of 48,000 +/- 2,000. The native molecular weight of 290,000 +/- 12,000 was calculated from determinations of the sedimentation coefficient, which was 11.3 S, and the Stokes radius, which was 60 A. Cross-linking the protein with dimethyl suberimidate yielded six bands. We conclude that the enzyme consists of six identical subunits. The apparent pI was 4.9 and the amino acid composition was typical except for the absence of cysteine.
Assuntos
Proteínas de Bactérias/isolamento & purificação , Escherichia coli/enzimologia , Aminoácidos/análise , Fenômenos Químicos , Físico-Química , Eletroforese em Gel de Poliacrilamida , Escherichia coli/genética , Ponto Isoelétrico , Métodos , Peso Molecular , Monoéster Fosfórico Hidrolases/isolamento & purificaçãoRESUMO
The single-stranded DNA-dependent ribonucleoside triphosphatase activity of the Escherichia coli dnaB gene product was characterized. Purine ribonucleoside triphosphates were the preferred substrates, but all ribonucleoside triphosphates were cleaved at the gamma position to yield ribonucleoside diphosphates and Pi. The enzyme required Mg2+, which could be replaced by Mn2+ but with lower activity. The pH optimum was 7.5 in either Tris-HCl or phosphate buffer. The Km for MgATP was 0.59 mM and the Vmax was 8.7 nmol/min/microgram of protein at 30 degrees. The DNA requirement was best satisfied with either fd or phiX174 single-stranded DNA (Km 0.033 mM nucleotides); maximal rate of nucleoside diphosphate formation occurred with 1 dnaB molecule/fd or phiX174 single-stranded DNA molecule. The dnaB gene product was found to have hysteretic properties and the hysteresis appeared to be due to a dissociation and reassociation of the enzyme.