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Cytotherapy ; 18(8): 1025-1036, 2016 08.
Artigo em Inglês | MEDLINE | ID: mdl-27311799

RESUMO

BACKGROUND AIMS: Cell transplantation in patients suffering spinal cord injury (SCI) is in its initial stages, but currently there is confusion about the results because of the disparity in the techniques used, the route of administration, and the criteria for selecting patients. METHODS: We conducted a clinical trial involving 12 patients with complete and chronic paraplegia (average time of chronicity, 13.86 years; SD, 9.36). The characteristics of SCI in magnetic resonance imaging (MRI) were evaluated for a personalized local administration of expanded autologous bone marrow mesenchymal stromal cells (MSCs) supported in autologous plasma, with the number of MSCs ranging from 100 × 10(6) to 230 × 10(6). An additional 30 × 10(6) MSCs were administered 3 months later by lumbar puncture into the subarachnoid space. Outcomes were evaluated at 3, 6, 9 and 12 months after surgery through clinical, urodynamic, neurophysiological and neuroimaging studies. RESULTS: Cell transplantation is a safe procedure. All patients experienced improvement, primarily in sensitivity and sphincter control. Infralesional motor activity, according to clinical and neurophysiological studies, was obtained by more than 50% of the patients. Decreases in spasms and spasticity, and improved sexual function were also common findings. Clinical improvement seems to be dose-dependent but was not influenced by the chronicity of the SCI. CONCLUSION: Personalized cell therapy with MSCs is safe and leads to clear improvements in clinical aspects and quality of life for patients with complete and chronically established paraplegia.


Assuntos
Transplante de Células-Tronco Mesenquimais , Paraplegia/terapia , Medicina de Precisão/métodos , Traumatismos da Medula Espinal/terapia , Adulto , Transplante de Medula Óssea/efeitos adversos , Doença Crônica , Feminino , Humanos , Imageamento por Ressonância Magnética , Masculino , Transplante de Células-Tronco Mesenquimais/efeitos adversos , Células-Tronco Mesenquimais/citologia , Células-Tronco Mesenquimais/fisiologia , Pessoa de Meia-Idade , Paraplegia/diagnóstico , Paraplegia/etiologia , Paraplegia/patologia , Medicina de Precisão/efeitos adversos , Qualidade de Vida , Espanha , Traumatismos da Medula Espinal/complicações , Traumatismos da Medula Espinal/diagnóstico , Transplante Autólogo/efeitos adversos , Resultado do Tratamento
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