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1.
Nucleic Acids Res ; 51(1): 54-67, 2023 01 11.
Artigo em Inglês | MEDLINE | ID: mdl-36610789

RESUMO

Riboswitches are conserved non-coding domains in bacterial mRNA with gene regulation function that are essential for maintaining enzyme co-factor metabolism. Recently, the pnuC RNA motif was reported to selectively bind nicotinamide adenine dinucleotide (NAD+), defining a novel class of NAD+ riboswitches (NAD+-II) according to phylogenetic analysis. To reveal the three-dimensional architecture and the ligand-binding mode of this riboswitch, we solved the crystal structure of NAD+-II riboswitch in complex with NAD+. Strikingly and in contrast to class-I riboswitches that form a tight recognition pocket for the adenosine diphosphate (ADP) moiety of NAD+, the class-II riboswitches form a binding pocket for the nicotinamide mononucleotide (NMN) portion of NAD+ and display only unspecific interactions with the adenosine. We support this finding by an additional structure of the class-II RNA in complex with NMN alone. The structures define a novel RNA tertiary fold that was further confirmed by mutational analysis in combination with isothermal titration calorimetry (ITC), and 2-aminopurine-based fluorescence spectroscopic folding studies. Furthermore, we truncated the pnuC RNA motif to a short RNA helical scaffold with binding affinity comparable to the wild-type motif to allude to the potential of engineering the NAD+-II motif for biotechnological applications.


Assuntos
Riboswitch , NAD/metabolismo , Filogenia , Ligantes , RNA/genética
2.
Sci China Life Sci ; 66(1): 31-50, 2023 01.
Artigo em Inglês | MEDLINE | ID: mdl-36459353

RESUMO

Riboswitches are highly conserved RNA elements that located in the 5'-UTR of mRNAs, which undergo real-time structure conformational change to achieve the regulation of downstream gene expression by sensing their cognate ligands. S-adenosylmethionine (SAM) is a ubiquitous methyl donor for transmethylation reactions in all living organisms. SAM riboswitch is one of the most abundant riboswitches that bind to SAM with high affinity and selectivity, serving as regulatory modules in multiple metabolic pathways. To date, seven SAM-specific riboswitch classes that belong to four families, one SAM/SAH riboswitch and one SAH riboswitch have been identified. Each SAM riboswitch family has a well-organized tertiary core scaffold to support their unique ligand-specific binding pocket. In this review, we summarize the current research progress on the distribution, structure, ligand recognition and gene regulation mechanism of these SAM-related riboswitch families, and further discuss their evolutionary prospects and potential applications.


Assuntos
Riboswitch , Humanos , Riboswitch/genética , S-Adenosilmetionina/química , S-Adenosilmetionina/metabolismo , Ligantes , RNA , Evolução Biológica , Conformação de Ácido Nucleico
3.
Nat Commun ; 10(1): 5728, 2019 12 16.
Artigo em Inglês | MEDLINE | ID: mdl-31844059

RESUMO

Riboswitches are metabolite-sensing, conserved domains located in non-coding regions of mRNA that are central to regulation of gene expression. Here we report the first three-dimensional structure of the recently discovered S-adenosyl-L-methionine responsive SAM-VI riboswitch. SAM-VI adopts a unique fold and ligand pocket that are distinct from all other known SAM riboswitch classes. The ligand binds to the junctional region with its adenine tightly intercalated and Hoogsteen base-paired. Furthermore, we reveal the ligand discrimination mode of SAM-VI by additional X-ray structures of this riboswitch bound to S-adenosyl-L-homocysteine and a synthetic ligand mimic, in combination with isothermal titration calorimetry and fluorescence spectroscopy to explore binding thermodynamics and kinetics. The structure is further evaluated by analysis of ligand binding to SAM-VI mutants. It thus provides a thorough basis for developing synthetic SAM cofactors for applications in chemical and synthetic RNA biology.


Assuntos
Bifidobacterium/genética , Modelos Moleculares , Conformação de Ácido Nucleico , RNA Bacteriano/ultraestrutura , Riboswitch/genética , Cristalografia por Raios X , Ligantes , RNA Bacteriano/genética , S-Adenosilmetionina/metabolismo
4.
Angew Chem Int Ed Engl ; 56(50): 15954-15958, 2017 12 11.
Artigo em Inglês | MEDLINE | ID: mdl-29098759

RESUMO

The pistol RNA motif represents a new class of self-cleaving ribozymes of yet unknown biological function. Our recent crystal structure of a pre-catalytic state of this RNA shows guanosine G40 and adenosine A32 close to the G53-U54 cleavage site. While the N1 of G40 is within 3.4 Šof the modeled G53 2'-OH group that attacks the scissile phosphate, thus suggesting a direct role in general acid-base catalysis, the function of A32 is less clear. We present evidence from atom-specific mutagenesis that neither the N1 nor N3 base positions of A32 are involved in catalysis. By contrast, the ribose 2'-OH of A32 seems crucial for the proper positioning of G40 through a H-bond network that involves G42 as a bridging unit between A32 and G40. We also found that disruption of the inner-sphere coordination of the active-site Mg2+ cation to N7 of G33 makes the ribozyme drastically slower. A mechanistic proposal is suggested, with A32 playing a structural role and hydrated Mg2+ playing a catalytic role in cleavage.


Assuntos
Adenosina/metabolismo , Biocatálise , Magnésio/metabolismo , RNA Catalítico/genética , RNA Catalítico/metabolismo , Adenosina/química , Domínio Catalítico , Magnésio/química , Mutagênese , Conformação Proteica , RNA Catalítico/química
5.
RNA ; 23(5): 655-672, 2017 05.
Artigo em Inglês | MEDLINE | ID: mdl-28138060

RESUMO

RNA-Puzzles is a collective experiment in blind 3D RNA structure prediction. We report here a third round of RNA-Puzzles. Five puzzles, 4, 8, 12, 13, 14, all structures of riboswitch aptamers and puzzle 7, a ribozyme structure, are included in this round of the experiment. The riboswitch structures include biological binding sites for small molecules (S-adenosyl methionine, cyclic diadenosine monophosphate, 5-amino 4-imidazole carboxamide riboside 5'-triphosphate, glutamine) and proteins (YbxF), and one set describes large conformational changes between ligand-free and ligand-bound states. The Varkud satellite ribozyme is the most recently solved structure of a known large ribozyme. All puzzles have established biological functions and require structural understanding to appreciate their molecular mechanisms. Through the use of fast-track experimental data, including multidimensional chemical mapping, and accurate prediction of RNA secondary structure, a large portion of the contacts in 3D have been predicted correctly leading to similar topologies for the top ranking predictions. Template-based and homology-derived predictions could predict structures to particularly high accuracies. However, achieving biological insights from de novo prediction of RNA 3D structures still depends on the size and complexity of the RNA. Blind computational predictions of RNA structures already appear to provide useful structural information in many cases. Similar to the previous RNA-Puzzles Round II experiment, the prediction of non-Watson-Crick interactions and the observed high atomic clash scores reveal a notable need for an algorithm of improvement. All prediction models and assessment results are available at http://ahsoka.u-strasbg.fr/rnapuzzles/.


Assuntos
RNA Catalítico/química , Riboswitch , Aminoimidazol Carboxamida/química , Aminoimidazol Carboxamida/metabolismo , Aptâmeros de Nucleotídeos/química , Aptâmeros de Nucleotídeos/metabolismo , Fosfatos de Dinucleosídeos/metabolismo , Endorribonucleases/química , Endorribonucleases/metabolismo , Glutamina/química , Glutamina/metabolismo , Ligantes , Modelos Moleculares , Conformação de Ácido Nucleico , RNA Catalítico/metabolismo , Ribonucleotídeos/química , Ribonucleotídeos/metabolismo , S-Adenosilmetionina/química , S-Adenosilmetionina/metabolismo
6.
Nat Chem Biol ; 10(9): 780-6, 2014 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-25086509

RESUMO

The ydaO riboswitch, involved in sporulation, osmotic stress responses and cell wall metabolism, targets the second messenger cyclic-di-AMP with subnanomolar affinity. We have solved the structure of c-di-AMP bound to the Thermoanaerobacter tengcongensis ydaO riboswitch, thereby identifying a five-helical scaffold containing a zippered-up bubble, a pseudoknot and long-range tertiary base pairs. Highlights include the identification of two c-di-AMP binding pockets on the same face of the riboswitch, related by pseudo-two-fold symmetry, with potential for cross-talk between sites mediated by adjacently positioned base-stacking alignments connecting pockets. The adenine rings of bound c-di-AMP molecules are wedged between bases and stabilized by stacking, base-sugar and sugar-sugar intermolecular hydrogen bonding interactions. The structural studies are complemented by isothermal titration calorimetry-based binding studies of mutants mediating key tertiary intermolecular contacts. The T. tengcongensis ydaO riboswitch, like its Bacillus subtilis counterpart, most likely functions through a transcription termination mechanism, with the c-di-AMP bound state representing an 'off' switch.


Assuntos
Fosfatos de Dinucleosídeos/genética , Genes Bacterianos/genética , Riboswitch/genética , Bacillus subtilis/química , Bacillus subtilis/metabolismo , Calorimetria , Cristalografia por Raios X , Fosfatos de Dinucleosídeos/metabolismo , Genes de Troca/genética , Ligantes , Modelos Moleculares , Conformação de Ácido Nucleico , RNA Bacteriano/genética , Sistemas do Segundo Mensageiro/genética , Thermoanaerobacter/genética , Thermoanaerobacter/metabolismo
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