Target-driven switch-on fluorescence aptasensor for trace aflatoxin B1 determination based on highly fluorescent ternary CdZnTe quantum dots.

Development of sensitive methods for trace aflatoxin B1 (AFB1) determination is of great significance due to its high toxicity and carcinogenicity. Herein, 3-mercaptopropionic acid (MPA)-capped ternary CdZnTe quantum dots (QDs) have been prepared via a simple hydrothermal route. We found that they exhibited enhanced intensity when benchmarked against their binary counterpart CdTe QDs. On this basis, a target-driven switch-on fluorescence aptasensor for trace AFB1 determination has been developed by employing the fluorescence resonance energy transfer (FRET) between the CdZnTe QDs and Au nanoparticles (AuNPs) pair. In the detection diagram, amino group-functionalized aptamers against AFB1 were firstly labelled with the CdZnTe QDs donors coated on silica nanospheres while the AuNPs acceptors were bioconjugated with the thiol group-modified complementary DNA (cDNA) of aptamer. By taking advantage of the DNA hybridization of aptamer and cDNA, the CdZnTe QDs (energy donor) and AuNPs (energy acceptor) were brought into close proximity, thereby leading to the occurrence of FRET during the aptasensor fabrication. When the aptasensor was incubated with AFB1, the specific binding between aptamer and target resulted in the detachment of AuNPs acceptors. This behavior would disturb the FRET process and led to the subsequent fluorescence recovery of CdZnTe QDs. Such designed aptasensor showed an increased fluorescence recovery upon the increasing concentration of AFB1 over a broad range of 50 pg mL-1 - 100 ng mL-1 and succeeded in spiked peanut samples. The proposed aptasensor is separation-free and easy-to-use, which might open up new possibilities in aptasensor fabrication by employing the novel CdZnTe QDs-AuNPs pair.

Fabrication of magnetically assembled aptasensing device for label-free determination of aflatoxin B1 based on EIS.

Aflatoxin B1 (AFB1), one of the most common mycotoxins in food matrixes, has been identified as the most toxic contaminant with mutagenic, teratogenic, immunosuppressive, and carcinogenic effects. In this work, a magnetically assembled aptasensing device has been designed for label-free determination of AFB1 by employing a disposable screen-printed carbon electrode (SPCE) covered with a designed polydimethylsiloxane (PDMS) film as the micro electrolytic cell. The magnetically controlled bio-probes were firstly prepared by immobilization of the thiolated aptamers on the Fe3O4@Au magnetic beads, which was rapidly assembled on the working electrode of SPCE within 10 s, by using a magnet placed at the opposite side. The PDMS film with a centered hole was covered on the SPCE surface to achieve a more practicable and flexible electrochemical measurement. In this effort, a label-free aptasensor for the sensitive and selective determination of AFB1 has been developed using electrochemical impedance spectroscopy upon the biorecognition between aptamers and the targets. The developed method had a wide linear range of 20 pg mL-1-50 ng mL-1 with a detection limit of 15 pg mL-1 (S/N = 3) and succeeded in spiked samples of peanuts. The developed aptasensing device shows fantastic application prospect with simple design, easy operation, low cost, and high sensitivity and selectivity characteristics. This sensing strategy represents a promising path toward routine quality control of food safety and creates the opportunity to develop facile aptasensing device for other targets.