Empatía con el paciente en médicos especialistas que trabajan en un Hospital de Cuenca (Ecuador) / Empathy with the Patient in Medical Specialists Working in a Hospital in Cuenca ­ Ecuador / Empatia com o paciente em médicos especialistas que trabalham em um hospital em Cuenca, Equador

ntroducción: la empatía es uno de los componentes de la estructura de la humanización en la atención de los pacientes. Los médicos especialistas deben tener mucha empatía. El objetivo del artículo es des-cribir los índices de empatía en médicos que trabajan en un hospital de alta complejidad y explorar si existen diferencias entre las especialidades estudiadas. Métodos: se accedió a una muestra por conve-niencia de 237 médicos que representa el 53.55 % del total poblacional (n = 443). La empatía se midió con la Escala de Empatía para Profesionales de la Salud (hp), junto con análisis factorial confirmatorio y análisis multigrupo (para examinar la invarianza del modelo entre los sexos) y análisis de confia-bilidad (α de Cronbach, coeficiente de correlación intraclase y ω de McDonald). Resultados: la espe-cialidad de pediatría tuvo los mayores índices de empatía general y en las dimensiones "cuidado con compasión" y "caminando en los zapatos del paciente", no así en la dimensión "toma de perspectiva", donde es semejante a las especialidades de anestesiología, medicina crítica y terapia intensiva, y gine-cología. Conclusión: los valores de la empatía y los valores en las subescalas "cuidado con compasión" y "caminando en los zapatos del paciente" fueron (en valores absolutos, pero no estadísticos) mayores en la especialidad de pediatría. En la subescala "toma de perspectiva", las especialidades de pediatría, anestesiología, cirugía y clínica se observaron puntuaciones prácticamente iguales. Se requieren otros estudios que permita arribar a una explicación que permita entender por qué algunas especialidades tienen valores de empatía mayores que otras

Introduction: Empathy is one of the components of the structure of humanization in patient care. Medical specialists must have high levels of empathy. Objective: The aim of this paper is to describe the levels of empathy in doctors working in a highly complex hospital and to explore whether there are differ-ences between the specialties studied. Methods: A convenience sample of 237 physicians representing 53.55% of the total population (n = 443) was accessed. Empathy was measured using the Empathy Scale for Health Professionals (hp). Confirmatory factor analysis and multigroup analysis were performed to examine the invariance of the model between the sexes and reliability analyzes (Cronbach's α, intraclass correlation coefficient and McDonald's ω). Results: The specialty of pediatrics had the highest levels in general empathy and in the Compassionate Care and Walking in Patient Shoes subscales, but not in the Perspective Taking subescale where it presents levels similar to the specialties of anesthesiology, critical medicine and intensive care. and gynecology. Conclusions: The empathy values and the values in the compassionate care and "Walking in the patient's shoes" subscales were (in absolute values, but not sta-tistically) higher in the Pediatrics specialty. In the subscale Perspective Taking, Pediatrics, Anesthesiology, Surgery, and Clinic, practically the same scores were observed. Other studies are required to arrive at an explanation that allows us to understand why some specialties have higher empathy values than others.

Introdução: a empatia é um dos componentes da estrutura de humanização no atendimento ao paciente. Os médicos especialistas devem ter altos níveis de empatia. Objetivo: o objetivo deste artigo é descre-ver os níveis de empatia em médicos que trabalham em um hospital de alta complexidade e explorar se há diferenças entre as especialidades estudadas. Materiais e métodos: foi acessada uma amostra de conveniência de 237 médicos, representando 53,55% da população total (n = 443). A empatia foi medida usando a Escala de Empatia para Profissionais de Saúde. A análise fatorial confirmatória e a análise mul-tigrupo foram realizadas (para examinar a invariância do modelo entre os sexos) e a análise de confia-bilidade (α de Cronbach, coeficiente de correlação intraclasse e ω de McDonald). Resultados: a pediatria apresentou os níveis mais altos de empatia geral e nas dimensões "cuidado compassivo" e "estar no lugar do paciente", mas não na dimensão "tomada de perspectiva", em que os níveis foram semelhantes aos da anestesiologia, da medicina crítica e da terapia intensiva, e da ginecologia. Conclusões: os valores de empatia e os valores das subescalas "cuidado compassivo" e "estar no lugar do paciente" foram (em valo-res absolutos, mas não estatisticamente) mais altos na especialidade de pediatria. Na subescala "tomada de perspectiva", as especialidades de pediatria, anestesiologia, cirurgia e clínica tiveram pontuações quase iguais. São necessários mais estudos para explicar por que algumas especialidades têm valores de empatia mais altos do que outras

Family functioning in students of health sciences in four Latin American countries: a study of the structure and factorial invariance of the FACES III scale. A cross-sectional study

Abstract Background Psychometric studies of the FACES III scale in Spanish-speaking countries show a lack of agreement on the factorial structure of the scale. In addition, most of the studies have only performed exploratory analyses of its factorial structure. Objective The objective of the present study was to confirm the structure and factorial invariance of the FACES III scale in nursing and obstetric students from Chile, Colombia, Peru, and Mexico. Methods A total of 3303 students from the four countries participated in this study (Colombia = 1559, Chile = 1224, Peru = 215, Mexico = 305). Results The results of the study showed that the Bi-factor model presents the best-fit indexes to the data from Colombia, Chile, and Mexico, but not from Peru. In addition, it was found that this model showed evidence of being strictly invariant among the three countries in the sequence of the invariance models proposed: metric invariance (ΔRMSEA = .000), scalar (ΔRMSEA = .008), and strict (ΔRMSEA = .008). The bi-factor model also showed adequate reliability indexes in the three countries. Conclusion It is concluded that the FACES III scale shows adequate psychometric performance under a bi-factor model in nursing and obstetric students from Colombia, Chile, and Mexico. The lack of fit of the model in Peru could be associated with the small sample size.

La empatía de los enfermeros con los pacientes en los hospitales públicos / Working nurses' empathy with patients in public hospitals / A empatia dos enfermeiros com os pacientes em hospitais públicos

Objetivo: determinar los niveles de empatía en profesionales de enfermería de un hospital de alta complejidad, relacionar la edad con la empatía (y cada una de sus dimensiones), y establecer si existen diferencias entre estos niveles según el tipo de jornada laboral. Método: diseño comparativo, correlacional y transversal. La muestra utilizada (n=271) constituyó el 40,9% del total de profesionales de enfermería. Se estudiaron las propiedades psicométricas de la Escala de Empatía de Jefferson para Profesionales de la Salud. Se calcularon estadísticos descriptivos: media y desviación estándar. La asociación entre empatía y edad se estimó mediante ecuaciones de regresión y significancia estadística de los coeficientes de regresión, luego de evaluar el tipo de curva mediante análisis de varianza. Resultados: se identificó el modelo subyacente de las tres dimensiones de la empatía. Los valores de los estadísticos descriptivos observados fueron relativamente bajos en empatía y sus dimensiones. Los niveles de empatía no se asociaron con el rango de edad. No se encontraron diferencias en la empatía entre los tipos de horarios de trabajo. Se encontró variabilidad en las dimensiones: "cuidado compasivo" y "ponerse en los zapatos del paciente". Conclusión: estos resultados muestran que los niveles de empatía observados pueden implicar un desempeño deficiente en el cuidado empático de los pacientes.

Objective: to determine the levels of empathy in professional nurses of a high-complexity hospital, to relate age to empathy (and each one of its dimensions), and to establish if there are differences between these levels according to the type of working schedules. Method: comparative, correlational and cross-sectional design. The sample used (n=271) constituted 40.9% of the total number of nursing professionals. Psychometric properties of the Jefferson Scale of Empathy for Health Professionals were studied. Descriptive statistics were calculated: mean and standard deviation. The association between empathy and age was estimated using regression equations and statistical significance of the regression coefficients, after evaluating the type of curve using variance analysis. Results: the underlying model of three dimensions of empathy was identified. The values of the descriptive statistics observed were relatively low in empathy and its dimensions. Empathy levels were not associated with the age range. No differences in empathy were found between the types of work schedules. Variability was found in the dimensions: "compassionate care" and "Walking on the patient's shoes". Conclusion: these results show that the levels of empathy observed may imply a deficient performance in empathetic care for patients.

Objetivo: determinar os níveis de empatia em enfermeiros profissionais de um hospital de alta complexidade, relacionar a idade com a empatia (e cada uma das suas dimensões) e verificar se existem diferenças entre esses níveis, de acordo com o tipo de horário de trabalho. Método: delineamento comparativo, correlacional e transversal. A amostra utilizada (n=271) constituiu 40,9% do total de profissionais de enfermagem. Foram estudadas as propriedades psicométricas da Escala de Empatia de Jefferson para Profissionais da Saúde. Foram calculadas estatísticas descritivas: média e desvio padrão. A associação entre empatia e idade foi estimada por meio de equações de regressão e significância estatística dos coeficientes de regressão, após avaliação do tipo de curva por meio de análise de variância. Resultados: o modelo subjacente de três dimensões de empatia foi identificado. Os valores das estatísticas descritivas observados foram relativamente baixos em empatia e suas dimensões. Níveis de empatia não foram associados com a faixa etária. Não foram encontradas diferenças de empatia entre os tipos de horários de trabalho. Foi encontrada variabilidade nas dimensões: "cuidado compassivo" e "colocar-se no lugar do paciente". Conclusão: esses resultados mostram que os níveis de empatia observados podem implicar em um desempenho deficiente no atendimento empático aos pacientes.

Apobec-Mediated Retroviral Hypermutation In Vivo is Dependent on Mouse Strain.

Replication of the complex retrovirus mouse mammary tumor virus (MMTV) is antagonized by murine Apobec3 (mA3), a member of the Apobec family of cytidine deaminases. We have shown that MMTV-encoded Rem protein inhibits proviral mutagenesis by the Apobec enzyme, activation-induced cytidine deaminase (AID) during viral replication in BALB/c mice. To further study the role of Rem in vivo , we have infected C57BL/6 (B6) mice with a superantigen-independent lymphomagenic strain of MMTV (TBLV-WT) or a mutant strain (TBLV-SD) that is defective in Rem and its cleavage product Rem-CT. Unlike MMTV, TBLV induced T-cell tumors in µMT mice, indicating that mature B cells, which express the highest AID levels, are not required for TBLV replication. Compared to BALB/c, B6 mice were more susceptible to TBLV infection and tumorigenesis. The lack of Rem expression accelerated B6 tumorigenesis at limiting doses compared to TBLV-WT in either wild-type B6 or AID-deficient mice. However, unlike proviruses from BALB/c mice, high-throughput sequencing indicated that proviral G-to-A or C-to-T changes did not significantly differ in the presence and absence of Rem expression. Ex vivo stimulation showed higher levels of mA3 relative to AID in B6 compared to BALB/c splenocytes, but effects of agonists differed in the two strains. RNA-Seq revealed increased transcripts related to growth factor and cytokine signaling in TBLV-SD-induced tumors relative to those from TBLV-WT, consistent with a third Rem function. Thus, Rem-mediated effects on tumorigenesis in B6 mice are independent of Apobec-mediated proviral hypermutation.

Family Functioning as a Possible Factor that Modulates Empathy Levels in Dental Students

Aim: The objective of this paper is to explore whether there are differences in the levels of empathy and its dimensions between family typologies in dental students. Methodology: A quantitative and not experimental study, with a cross-sectional descriptive design, a population of dental students from the Evangelical University of El Salvador, and a convenience sample were applied. The levels of empathy and family functioning were evaluated using the Jefferson Scale of Empathy for Health Professions students and the Brief Scale of Family Functioning. The construct validity of both instruments was estimated using Confirmatory Factor Analysis. The reliability was estimated using McDonald's omega and Cronbach's alpha. Comparisons of empathy and its dimensions between family typologies were made using a two-factor analysis of variance. Results: No statistical differences were observed in empathy and its dimensions based on family typologies. It was found that women are more empathic than men. This result is not in accordance with other studies carried out in Latin America on dental and medical students evaluating empathy and family functioning with the same instruments. Conclusions: The distribution of empathy levels and their dimensions are similar among the family typologies studied. Therefore, it was not found that family functioning can influence empathy in the population studied. It is necessary to continue these studies to obtain more empirical evidence regarding the influence of family functioning on empathy.

Objetivo: El objetivo del presente trabajo es determinar la existencia de diferencias de los niveles de empatía y de sus dimensiones entre las tipologías familiares en estudiantes de odontología. Metodología: Estudio cuantitativo y no experimental, con un diseño descriptivo transversal, una población de estudiantes de odontología de la Universidad Evangélica de El Salvador y una muestra por conveniencia. Fueron evaluados los niveles de empatía y el funcionamiento familiar mediante la Escala de Empatía de Jefferson para estudiantes de profesiones de la salud y la Escala Breve de Funcionamiento Familiar. Se estimó la validez de constructo de ambos instrumentos mediante Análisis Factorial Confirmatorio y la confiabilidad mediante omega de McDonald y alfa de Cronbach. Las comparaciones de la empatía y sus dimensiones entre las tipologías familiares fueron realizadas mediante análisis de varianza bifactorial. Resultados: No se observaron diferencias estadísticas en la empatía y en sus dimensiones en función de las tipologías familiares. Se encontró que las mujeres son más empáticas que los hombres. Este resultado no está en concordancia con otros estudios realizados en América Latina en estudiantes de odontología y medicina, evaluando la empatía y el funcionamiento familiar con los mismos instrumentos. Conclusiones: La distribución de los niveles de empatía y de sus dimensiones son semejantes entre las tipologías familiares estudiadas. Por lo tanto, no se constató que el funcionamiento familiar tenga un efecto sobre la empatía en la población estudiada. Es necesario continuar estos estudios para obtener mayor evidencia empírica respecto de la influencia del funcionamiento familiar en la empatía.

Validación del instrumento de conocimiento sobre enfermedad vascular cerebralen el personal de enfermería (GGA/NICEVC-22) / Validation of the knowledge instrument on cerebrovascular disease in nursing staff (GGA/NICEVC-22) / Validação do instrumento de conhecimento sobre doença cerebrovascular na equipe de enfermagem (GGA/NICEVC-22)

Objetivo: validar un instrumento que evalué el nivel conocimiento del personal de enfermería en la atención al paciente con enfermedad vascular cerebral. Método: estudio longitudinal-proceso con 115 profesionales de enfermería pertenecientes en un hospital de segundo nivel, realizado el periodo de diciembre 2019 a mayo 2020. Muestro no probabilístico por conveniencia. La validación del instrumento se hizo en cinco momentos:1) creación del constructo, 2) validación por 15 jueces expertos, 3) Prueba piloto del instrumento, 4) consistencia interna por el Alpha de Cronbach y 5) validación por análisis factorial con componente rotados, además se aplicó estadística descriptiva. Resultados: el análisis factorial mostró la distribución de los 22 ítems distribuidos en cuatro factores con una varianza explicada de 53.30% y una consistencia interna de α=0.89. Conclusión:los resultados permiten concluir que el instrumento presenta una validez y confiabilidad aceptables, el cual permite evaluar el nivel de conocimiento sobre EVC en el personal de enfermería.

Objective:validar um instrumento que avalia o nível de conhecimento da equipe de enfermagem no cuidado ao paciente com doença cerebrovascular. Method:longitudinal-process study with 115 nursing professionals belonging to a second level hospital, carried out from December 2019 to May 2020. Non-probabilistic sampling for convenience. The validation of the instrument was done in five moments: 1) creation of the construct, 2) validation by 15 expert judges, 3) pilot test of the instrument, 4) internal consistency by Cronbach's Alpha and 5) validation by factorial analysis with rotated components, descriptive statistics were also applied. Results:the factorial analysis showed the distribution of the 22 items distributed in four factors with an explained variance of 53.30% and an internal consistency of α=0.89. Conclusion:the results allow us to conclude that the instrument has acceptable validity and reliability, which allows us to evaluate the level of knowledge about CVD in the nursing staff.

Objetivo:validar um instrumento que avalia o nível de conhecimento da equipe de enfermagem no cuidado ao paciente com doença cerebrovascular. Método:estudode processo longitudinal com 115 profissionais de enfermagem pertencentes a um hospital de segundo nível, realizado no período de dezembro de 2019 a maio de 2020. Amostragem não probabilística por conveniência. A validação do instrumento foi feita em cinco momentos: 1) criação do construto, 2) validação por 15 juízes especialistas, 3) teste piloto do instrumento, 4) consistência interna pelo Alfa de Cronbach e 5) validação por análise fatorial com rodízio componentes., também foi aplicada estatística descritiva. Resultados:a análise fatorial mostrou a distribuição dos 22 itens distribuídos em quatro fatores com variância explicada de 53,30% e consistência interna de α=0,89. Conclusão: os resultados permitem concluir que o instrumento possui validade e confiabilidade aceitáveis, o que permite avaliar o nível de conhecimento sobre DCV na equipe de enfermagem.

Association of MIR3117 and MIR612 Genes Polymorphisms with Childhood Acute Lymphoblastic Leukemia in the Mexican Population.

INTRODUCTION: Acute lymphoblastic leukemia (ALL) is the most common childhood cancer in the world, which is associated with a wide spectrum of factors that play an important role in epidemiology, risk stratification, and therapeutic intervention. Several studies have shown the role of microRNAs (miRNAs) in the development of the disease. Genetic variations such as single-nucleotide polymorphisms (SNPs) in miRNAs can alter their function and lead to alter the expression of their target genes. OBJECTIVE: The aim of this study was to evaluate the association of rs12402181 in MIR3117 and rs12803915 in MIR612 with the risk of childhood preB-ALL in Mexican population. MATERIAL AND METHODS: DNA from 148 children (<18 years old) diagnosed with preB-ALL and 172 samples from participants in control group were included in the present study. Genotyping of the rs12402181 and rs12803915 polymorphisms was carried out by Real-Time PCR. To estimate the risk factor, the multiple genetic models co-dominant, dominant, and recessive were determined in both polymorphisms. RESULTS: In dominant genetic model from rs12402181, a high risk of susceptibility to ALL was observed (OR = 2.03, 95% CI = 1.27-3.22, p = 0.003). In the analysis adjusted for gender, a significant increase in the risk of ALL was maintained (OR = 2.03, 95% CI = 1.28-3.24, p = 0.003). The rs12803915 polymorphism was no associated with the risk of susceptibility to preB-ALL in any of the genetic models using in this study. CONCLUSIONS: Our data indicated that the A allele of the rs12402181 polymorphism may be considered as a genetic biomarker of preB-ALL susceptibility. Likewise, it was identified that the A allele of the rs12402181 polymorphism is an independent risk factor for ALL.

Empathy levels among dental students and professors from a dental school in the Dominican Republic / Empatía en estudiantes y profesores de una escuela de odontología de República Dominicana

Abstract Introduction: The concept of empathy has been incorporated as one of the key elements for the achievement of the teaching-learning process goals in health science students. Objective: To estimate and compare the levels of empathy among dental students and professors in the undergraduate dental medicine program at the Universidad Central del Este (Dominican Republic). Materials and methods: Cross-sectional study. The study population (n=264) was divided into two groups: the first consisted of students in their first to fifth year of dental school (N=223; n=215), distributed in two areas (basic-preclinical and clinical courses), while the second group comprised professors working in both areas in the dental school of the university (N=53; n=49). The Jefferson Scale of Empathy (S-Version) was used. The descriptive analysis of the data included the estimation of means, standard deviations and percentages, and the reliability of the data was estimated using Cronbach's alpha. In addition, a two-way ANOVA was performed, calculating the effect size and the statistical power of the test; furthermore, when the Fisher's exact test was significant for any factor, Tukey's test was used to estimate differences between means. A significance level of α<0.05 and β<0.20 was established. Results: Overall empathy scores and compassionate care dimension scores among the professor group did not differ significantly from the scores obtained by the students (basic-preclinical and clinical area), but there were differences between students from both areas (p<0.05). There were no significant differences between the three subgroups in the Perspective Taking and Walking in the Patient's Shoes dimensions (p=0.428 and p=0.866). Conclusion: The levels of empathy and compassionate care dimension of professors are similar to those of students in general (regardless of the area).

Resumen Introducción. El concepto de empatía se ha integrado como uno de los elementos centrales para el logro final del proceso de enseñanza-aprendizaje en estudiantes de ciencias de la salud. Objetivo. Estimar y comparar los niveles de empatía entre estudiantes y profesores de pregrado de odontología de la Universidad Central del Este (República Dominicana). Materiales y métodos. Estudio transversal. La población de estudio (n=264) se dividió en dos grupos: el primero, compuesto por estudiantes de primero a quinto año de la carrera de odontología (N=223; n=215) distribuidos en dos áreas (básica-preclínica y clínica), y el segundo, por los docentes de ambas áreas en la escuela de odontología de la universidad (N=53; n=49). Se utilizó la Escala de Empatía Médica de Jefferson (Versión-S). El análisis descriptivo de los datos incluyó la estimación de medias, desviaciones estándar y porcentajes, y la confiabilidad de los datos se estimó mediante CC de Cronbach; además, se realizó un ANOVA bifactorial, calculándose el tamaño del efecto y la potencia de la prueba, y en los casos en que la prueba exacta de Fisher fue significativa para algún factor, se utilizó la prueba de Tukey para estimar las diferencias entre las medias. El nivel de significancia estadística utilizado fue α<0.05 y β<0.20. Resultados. Los valores globales de empatía y de la dimensión Cuidado con compasión en los profesores no difirieron significativamente con los obtenidos por los estudiantes (área básica-preclínica y clínica), pero sí hubo diferencias entre los estudiantes de ambas áreas (p<0.05). En las dimensiones Adopción de perspectiva y Ponerse en los zapatos del otro no existieron diferencias entre los tres subgrupos (p=0.428 y p=0.866). Conclusión. Los niveles de empatía y de la dimensión Cuidado compasivo de los profesores no difieren de los de los estudiantes en general (ambas áreas).

Tuberculosis: el contrapunto del progreso / Tuberculosis: the counterpoint of progress

Resumen A lo largo de la historia, las enfermedades infecciosas han influido directamente en el desarrollo de la humanidad y en este proceso, la tuberculosis ha tenido un rol protagónico. Esta enfermedad mata más seres humanos que cualquier otra de causa infecciosa y, a pesar de esto, continúa siendo una entidad olvidada y un grave problema de salud pública, sobre todo en las naciones más pobres. La trascendencia de la tuberculosis va más allá del ámbito médico y una visión holística de ella nos permite comprender el grado de desarrollo económico y social de un Estado. Si bien Chile mantenía un programa de control históricamente exitoso, las cifras actuales no son auspiciosas y obligan a analizar el problema desde una mirada multidisciplinaria. Es en este marco que planteamos que el médico clínico, para aportar en el control de la enfermedad, debe poner nuevamente en práctica uno de los principios básicos de la Medicina: la semiología.

Abstract Along history, infectious diseases have had a direct influence in the development of humanity, with tuberculosis showing a leading role. Despite this disease being the main cause of mortality among infectious diseases, it remains neglected and constitutes a serious public health problem, especially among the poorest countries in the world. Tuberculosis greatest importance goes beyond Medicine, and a holistic view of the disease allows us to comprehend the economic and social development of a nation. Despite a historically successful control program in Chile, current figures are not auspicious and force upon us the need to address this problem with a multidisciplinary approach. The medical physician is required to put again into practice the fundamental principle of Medicine, Semiology to contribute to the control of tuberculosis.

Metabolic changes induced by DNA damage in Ramos cells: exploring the role of mTORC1 complex.

DNA damage induces the activation of many different signals associated with repair or cell death, but it is also connected with physiological events, such as adult neurogenesis and B-cell differentiation. DNA damage induces different signaling pathways, some of them linked to important metabolic changes. The mTORC1 pathway has a central role in the regulation of growth processes and cell division in response to environmental changes and also controls protein synthesis, lipid biogenesis, nucleotide synthesis, and expression of glycolytic genes. Here, we report that double-strand breaks induced with etoposide affect the expression of genes encoding different enzymes associated with specific metabolic pathways in Ramos cells. We also analyzed the role of mTOR signaling, demonstrating that double-strand breaks induce downregulation of mTOR signaling. Specific inhibition of mTORC1 using rapamycin also induced changes in the expression of metabolic genes. Finally, we demonstrated that DNA damage and rapamycin can regulate glucose uptake. In summary, our findings show that etoposide and rapamycin affect the expression of metabolic genes as well as apoptotic and proliferation markers in Ramos cells, increasing our understanding of cancer metabolism.

Variability of empathy among dental students. Implications not yet understood in Latin America / Variabilidad empática en estudiantes de odontología. Consecuencias aún no entendidas en América Latina

Abstract Introduction: Empathy is a quality that allows dentists to build an intersubjective relationship with their patients, which, among other benefits, contributes to the effectiveness of the treatment. Objective: To determine whether there is variability in empathy levels between two populations of dental students and to describe theoretically the general implications of this variability for intervention strategies. Materials and methods: Exploratory cross-sectional study. The study population consisted of 1st-5th year dental students from the Universidad Santiago de Cali, Colombia (n=610; N=647) and the Universidad San Sebastián, Chile (n=535; N=800). In both groups, empathy was measured using the Jefferson Scale of Empathy (S-Version) Scale. Descriptive statistics (mean and standard deviation) were used for data analysis. Internal consistency of data was estimated using Cronbach's alpha and the intraclass correlation coefficient. A factorial analysis of variance was performed, and three factors were studied: University (U), Course (C), and Sex (S). The statistical significance level used was α≤ 0.05 and β ≤0.20. Results: Differences in empathy level and in some of its three dimensions were observed between students from both universities and among courses (lst-5th year). No differences were found between sexes. Conclusions: There is variability in empathy levels among dental students from both universities. Thus, the implementation of specific empathy intervention strategies in each dental medicine program offered in Latin America is required to increase empathy levels in this population.

Resumen Introducción. La empatía es un atributo que permite a los odontólogos establecer una relación intersubjetiva con sus pacientes, lo que contribuye a un tratamiento exitoso, entre otros beneficios. Objetivo. Determinar si hay variabilidad en los niveles de empatía entre dos poblaciones de estudiantes de odontología y describir teóricamente las implicaciones generales de esta variabilidad en estrategias de intervención. Materiales y métodos. Estudio exploratorio transversal. La población de estudio consistió de estudiantes de odontología de 1er a 5to año de la Universidad Santiago de Cali, Colombia (n=610; N=647) y la Universidad San Sebastián, Chile (n=535; N=800). En ambos grupos, la empatía se midió con la Escala de Empatía Médica de Jefferson (Versión S). Para el análisis de los datos se utilizó estadística descriptiva (media y desviación estándar). La consistencia interna de los datos se estimó mediante el coeficiente alfa de Cronbach y el coeficiente de correlación intraclase. Se realizó un análisis de varianza factorial: tres factores estudiados: Universidad (U), Curso (C) y Sexo (S). El nivel de significancia estadística utilizado fue de α≤0.05 y β≤0.20. Resultados. Se observaron diferencias en el nivel de empatía y algunas de sus tres dimensiones entre los estudiantes de ambas universidades y entre los cursos (1er-5to año). No se observaron diferencias entre sexos. Conclusiones. Existe variabilidad en los niveles de empatía entre los estudiantes de ambas universidades. Para aumentar los niveles de empatía en esta población en Latinoamérica se requiere implementar estrategias específicas de intervención empática en cada programa de odontología ofrecido en la región.

Empathy in Professors and Students of a School of Dentistry in the Caribbean

Abstract Objective: To estimate and compare the levels of empathy between undergraduate dentistry students and professors at a university in the Dominican Republic. Material and Methods: Cross-sectional and descriptive study. The studied population consisted of two groups. The first: students of the Dentistry Career (N=520; n=335: 64.42% of total students) were distributed in two areas, basic-preclinical and clinical, while the second group corresponded of teachers who work in both areas (N=92; n=56; 60.87% of all teachers). The total sample was n = 391. The Jefferson Scale of Physician Empathy (S-Version) was used. Reliability was estimated using Cronbach's α and intraclass correlation coefficient, descriptive statistics, two-way analysis of variance, Tukey's test, effect size, and power of the test. Significance level: α≤0.05 and β≤0.20. Results: The empathy and dimension values were, in general, higher in the professors of the clinical area in relation to the other areas studied, with the exception of the compassionate care dimension. Conclusion: The finding that clinical teachers have a greater value of empathy is potentially an advantage for training students, especially in the clinical area (AU).

Sensibilidad ética en estudiantes versus profesionales de enfermería / Ethical sensitivity in nursing students and professionals / Sensibilidade ética em estudantes e profissionais de enfermagem

RESUMEN Objetivo: comparar la sensibilidad ética de estudiantes de una universidad privada del estado de Puebla versus profesionales de enfermería adscritos a instituciones de salud. Método: comparativo, prospectivo y de corte transversal, en 217participantes divididos en 109 para estudiantes y 108 para profesionales. Se aplicó el instrumento de sensibilidad ética. Se aplico estadística descriptiva y ANOVA de un factor. Resultados:la sensibilidad etica de las enfermeras y enfermeros en general es alta (= 73.14 ±15.38); Sin embargo, en relación al grado académico el promedio de la sensibilidad ética fue más alto en la licenciatura en enfermería (= 74.41 ±14.47) y posgrado en enfermería (= 73.77 ±11.57) en comparación el postécnico en enfermería (= 69.78 ±18.22). Al realizar el análisis de varianza de la ANOVA de un factor,se muestra que no existe diferencia entre el grado académico y la sensibilidad ética (F= 1.865, p = .167). Conclusión: la sensibilidad ética en estudiantes como profesionales de enfermería es alta y no cambia a pesar de que estén en ámbitos hospitalarios o universitarios, por lo que en la profesión de enfermería existen valores consistentes en sus diferentes ámbitos.

ABSTRACTObjective:to compare the ethical sensitivity of students from a Public University of the state of Puebla versus nursing professionals assigned to health institutions. Method:comparative, prospective, cross-sectional, in 217 participants divided into 109 for students and 108 for professionals. The instrument of ethical sensitivity was applied. Descriptive statistics and one-way ANOVA were applied. Results:the Ethical Sensitivity of Nurses in general is high (X̅= 73.14 ±15.38); However, in relation to the academic degree, the average ethical sensitivity was higher in the undergraduate nursing (X̅= 74.41 ±14.47) and postgraduate in nursing (X̅= 73.77 ±11.57) compared to the post-technical in nursing (X̅= 69.78 ±18.22). When performing the one-way ANOVA analysis of variance, it is shown that there is no difference between academic grade and ethical sensitivity (F = 1.865, p = .167). Conclusion: the ethical sensitivity in students as nursing professionalsis high and does not change even though they are in hospital or university settings, so that in the nursing profession there are consistent values in its different fields.

RESUMOObjetivo:comparar a sensibilidade ética de estudantes de uma universidade privada do estado de Puebla com profissionais de enfermagem designados a instituições de saúde. Método:comparativo, prospectivo y transversal, com 217 participantes divididos em 109 estudantes e 108 profissionais. Foi aplicado o instrumento de sensibilidade ética. Estatísticas descritivas e ANOVA de uma via foram aplicadas. Resultados:a sensibilidade ética dos enfermeiros em geral é alta (X̅= 73,14 ±15,38), porém, em relação ao grau acadêmico, a média da sensibilidade ética foi maior na graduação em enfermagem (X̅= 74,41 ±14,47) e na pós-graduação em enfermagem (X̅= 73,77 ±11,57) em comparação àpós-técnica em enfermagem (X̅= 69,78±18,22). Ao realizar a análise de variância ANOVA de um fator, émostrado que não hádiferença entre a nota acadêmica e a sensibilidade ética (F = 1,865, p = 0,167). Conclusão:a sensibilidade ética dos estudantes como profissionais de enfermagem é elevada e não se altera mesmo estando em ambientes hospitalares ou universitários, de modo que na profissão de enfermagem existem valores consistentes em suas diferentes áreas.

Decline in empathy levels and sex differences in medical students from the Caribbean / Declinación empática y diferencias según el sexo en estudiantes de medicina del Caribe

Abstract Introduction: Empathy is an important trait in the training of medical students, as it has been shown that it improves the doctor-patient relationship. Objective: To evaluate the decline of empathy levels and possible sex differences in undergraduate medical students from the Universidad Central del Este, Dominican Republic. Materials and methods: Exploratory cross-sectional study. A Spanish version of the Jefferson Scale of Empathy for Medical Students (S-version) Scale was administered in September 2018 to 1 144 1st-year to 5th-year medical students (887 women and 257 men). Data reliability was verified using the Cronbach's alpha and the intraclass correlation coefficient (ICC). A generalized linear equation model (Type III) was applied to analyze data and the Wald chi-squared test was used to determine differences in overall empathy levels and the mean scores obtained in each of its three components based on the year of medical training and sex. Results: Cronbach's alpha was satisfactory (0.839), and the ICC was 0.834 (F=5.68; p=0.005). The variability of the estimated curves in relation to empathic behavior by course (year of medical training) and sex was observed using linear and non-linear regression equations: Wald x2=115.6, p=0.0001 between courses; and Wald x2 =12.85, p=0.001 between men and women. Conclusions: Sex differences were observed regarding empathy levels in the study population. Moreover, a decline in empathy levels (overall empathy and Compassionate Care component in men and Walking in the Patient's Shoes component in men and women) was also observed as students progressed in their medical training. The behavior of these data raises questions regarding the need to determine the factors causing these differences and the decline in empathy levels.

Resumen Introducción. La empatia es un rasgo importante en los estudiantes de medicina, ya que se ha demostrado que mejora la relación médico-paciente. Objetivo. Evaluar la declinación empática y las posibles diferencias según el sexo en los niveles de empatia en estudiantes de pregrado de Medicina en la Universidad Central del Este, República Dominicana. Materiales y métodos. Estudio exploratorio transversal. La versión en español de la Escala de Empatia de Jefferson adaptada para estudiantes de medicina (versión S) fue aplicada a 1 144 estudiantes de medicina de 1° a 5° año (887 mujeres y 257 hombres). La confiabilidad de los datos se verificó mediante el alfa de Cronbach y el coeficiente de correlación intraclase (CCI). Los datos se analizaron utilizando un modelo de ecuación lineal generalizada (Tipo III) y se utilizó la prueba x2 de Wald para determinar las diferencias en los niveles globales de empatia y los puntajes promedio de sus tres componentes según el año de formación y el sexo. Resultados. El alfa de Cronbach fue satisfactorio (0.839) y el CCI fue de 0.834 (F=5.68; p=0.005). Mediante ecuaciones de regresión lineal y no lineal se observó variabilidad de las curvas estimadas en relación con el comportamiento empático según el curso (año de formación médica) y el sexo: entre cursos: x2 de Wald= 115.6; p=0.000i, y entre hombres y mujeres: x2 de Wald= 12.85; p=0.001). Conclusiones. Se observaron diferencias en los niveles de empatia según el sexo; también se evidenció una declinación en los niveles de empatia (empatia global y componente Cuidado con compasión en los hombres, y en el componente Ponerse en los zapatos del otro en hombres y mujeres) a medida que los estudiantes avanzaban en su formación. El comportamiento de estos datos genera interrogantes relacionados con la necesidad de determinar los factores que causan estas diferencias y dicha declinación empática.

Pediatric rhegmatogenous retinal detachment: predictors of anatomic and functional success.

PURPOSE: This study describes presenting clinical features and surgical techniques associated with successful repair of pediatric rhegmatogenous retinal detachment (RRD). METHODS: This is a retrospective case series which involved 242 cases younger than 18 years with new-onset RRD with descriptive statistics for the full group. Further exclusion established 168 cases that underwent surgery with minimum 3-month follow-up. Comparison of features associated with successful outcomes was analyzed using Chi-squared tests, logistic regression and univariate generalized equation models. RESULTS: We measured proportion of patients with BCVA ≤ 1.0 logMAR and/or an increase in final BCVA of 0.3 logMAR with respect to baseline and complete reattachment at final visit; 104 eyes (62%) achieved total reattachment, and 91 eyes (54%) achieved visual success. Absence of macular involvement, subtotal RRD and older age group (13-18) were associated with both success measures. There were higher visual and anatomic success rates with primary scleral buckling (SB, 66% and 79%; OR 9.26 and 11.09) and combined SB plus pars plana vitrectomy (PPV, 54% and 58%; OR 5.67 and 3.94) compared with PPV alone (26% and 17%). CONCLUSION: A majority of patients achieved anatomical success with repair. Trauma and myopia were the most common etiologic associations, with myopic cases having better outcomes. Success was more likely in patients with subtotal RRD or uninvolved macula at presentation; previous intraocular surgery was a risk factor for failure. Younger patients had a higher likelihood of worse outcomes. Initial PPV showed a lower rate of success than either SB or combined SB/PPV.

Psicometria, escala de empatía médica de jefferson y atención médica en Ecuador / Psychometry, the Jefferson Medical Empathy Scale and Medical Care in Ecuador

RESUMEN Objetivo: Verificar que la estructura factorial de los datos observados estén en correspondencia con la estructura factorial teórica de la empatia. Estimar y comparar los niveles de empatía en las especialidades médicas evaluadas. Materiales y métodos: Estudio realizado en el cantón de Cuenca (Ecuador) en 2019. Diseño descriptivo y transversal. La muestra estuvo constituida por 223 médicos que trabajaban en el sector público, quienes respondieron voluntariamente la Escala de Empatia Médica de Jefferson. Variables dependientes: niveles de empatia y sus dimensiones. Independientes: género, nivel de atención y especialidad. Se estimó la normalidad y homocedasticidad, Análisis Factorial Confirmatorio, Invarianza entre grupos, análisis de varianza trifactorial y de un factor, t-Student y d de Cohen. El nivel de significación fue a< .05. Resultados: Se observa una adecuada consistencia interna, se confirma el modelo de tres dimensiones del instrumento original. No existen diferencias entre los géneros (p> .05), pero sí entre los tipos de atención y entre las especialidades (p< .05). Los datos observados tienen la estructura factorial teórica asociada al instrumento empleado en la medición de la empatía. Conclusión: El análisis multigrupo permite asumir invarianza factorial de la empatía entre los géneros, sin que se logre especificar un modelo según tipos de atención al paciente. Los niveles de empatía global fueron mayores en la atención primaria en relación con la hospitalaria y mayor en las especialidades de Pediatría y Medicina Familiar (unidas).

ABSTRACT Objective: To verify that the factorial structure of the data observed in doctors corresponds with the theoretical factorial structure of empathy. Estimate and compare the levels of empathy in the evaluated medical specialties. Materials and Methods: The study was carried out in the canton of Cuenca (Ecuador) in 2019. It has a descriptive and cross-sectional design. The sample consisted of 223 physidans who worked in the Public Sector, who voluntarily answered the Jefferson Medical Empathy Scale. Dependent variables: levels of empathy and their dimensions. Independent: gender, level of care, and specialty. Normality and homoscedasticity, Confirmatory Factor Analysis, Invariance between groups, trifactorial and one-factor analysis of variance, Student's t and Cohen's d were estimated. The level of significance was a< .05. Results: An adequate internal consistency is observed, the three-dimensional model of the original instrument is confirmed. There are no differences between genders (p> .05), but there are between types of care and between specialties (p <.05). Conclusion: The observed data have the theoretical factorial structure associated with the instrument used to measure empathy. The multigroup analysis makes it possible to assume factorial invariance of empathy between the genders without being able to specify a model according to types of patient care. The levels of global empathy were higher in primary care compared to hospital care and higher in the specialties of Pediatrics and Family Medicine (united).

High frequency of the risk allele of rs4132601 and rs11978267 from the IKZF1 gene in indigenous Mexican population.

BACKGROUND: IKZF1 is a relevant gene associated with the pathogenesis of acute lymphoblastic leukemia, and the rs4132601 (T>G) and rs11978267 (A>G) polymorphisms have been associated with the development of this disease in several populations. The aim of this study was to determine the allelic and genotypic frequencies of the rs4132601 and rs11978267 polymorphisms in two indigenous Mexican groups (Cora and Huichol) and Mestizo populations from Nayarit, Mexico, and compare them with the frequencies of both polymorphisms in other populations of the world. METHODS: One hundred, 116, and 100 subjects from the Mestizo, Huichol, and Cora populations, respectively, all of them residents of the state of Nayarit, Mexico, were analyzed. The frequencies of rs4132601 and rs11978267 were determined by allelic discrimination using TaqMan assays. RESULTS: The allelic frequencies of rs4132601 were as follows: Mestizo group T = 0.74, G = 0.26; Cora T = 0.745, G = 0.255; and Huichol T = 0.47, G = 0.53. In the case of the rs11978267 polymorphism, the allelic frequencies were Mestizo A = 0.745, G = 0.255; Cora A = 0.735, G = 0.265; and Huichol A = 0.457, G = 0.543. For each population, both polymorphisms were in Hardy-Weinberg equilibrium. CONCLUSION: The Huichol population from Nayarit presented the highest frequencies of the risk allele reported to date in the whole world for both rs4132601 and rs11978267 polymorphisms.

Neutrophil extracellular traps and inflammatory response: Implications for the immunopathogenesis of ankylosing spondylitis.

AIM: Ankylosing spondylitis (AS) pathogenesis has focused on the adaptive immune response; however, innate immune responses may also play a role in the inflammatory response of AS. Dysregulated neutrophil activation can induce tissue damage and contribute to the pathogenesis of immune-related diseases. Hence, the aim of this study was to assess the effect of immune complexes formed with the p30 of Salmonella typhimurium and anti-p30 antibodies present in the sera of AS patients and controls in inducing the release of neutrophil extracellular traps (NETs) and the secretion of pro-inflammatory cytokines. METHODS: We collected polymorphonuclear leukocytes (PMNs) from healthy donors. The PMNs isolated were stimulated with p30 alone or in immunocomplexes formed with antibodies presents in sera of AS patients or control subjects. Then, the NETs were analyzed by fluorescence microscopy. Concentrations of interleukin (IL)-6, tumor necrosis factor (TNF)-α, IL-1ß, IL-8 and IL-10, were determined using the Cytometric Bead Array kit. RESULTS: Significant difference was observed in the release of NETs between the neutrophils stimulated with p30 + AS (70.52 ± 16.24) those unstimulated neutrophils (9.94 ± 12.12; P = .0095), stimulated with phorbol 12-myristate 13-acetate (39.78 ± 14.50; P = .0190), stimulated with control serum (CS) (10.85 ± 5.33; P = .0082) and serum of AS patient (10.28 ± 6.15; P = .0087). The stimulation of neutrophils with p30 alone induced a relatively low production of IL-6 (64.5 pg/mL), IL-8 (2658.3 pg/mL), IL-1ß (31.11 pg/mL), and TNF-α (3.8 pg/mL), compared to p30 + AS and p30 + CS groups. CONCLUSION: Our results show that neutrophils release NETs and pro-inflammatory cytokines in response to p30 in immunocomplexes. These findings could improve our understanding of the role of innate immunity in the initiation and/or maintenance of inflammatory responses, and in the progression of AS.