1.
Chem Commun (Camb)
; 54(54): 7471-7474, 2018 Jul 11.
Artigo
em Inglês
| MEDLINE
| ID: mdl-29915829
RESUMO
Herein, we describe an extended version of a fluorescence probe for detecting miRNAs through the novel application of a PyA-cluster system. By testing various (CG)n sequences in the middle of the oligonucleotide strand of the probe, we obtained an optimal sequence that formed a double-three-way-junction structure, with two PyA units positioned close together, in the presence of the target miRNA. This system readily detected the locations of target miRNAs in living cells and allowed visualization of structural changes through variations in the color of the fluorescence.