Genetic variability profiling of the p53 signaling pathway in chronic lymphocytic leukemia. Individual and combined analysis of TP53, MDM2 and NQO1 gene variants.

TP53 gene disruption, including 17p13 deletion [del(17p)] and/or TP53 mutations, is a negative prognostic biomarker in chronic lymphocytic leukemia (CLL) associated with disease progression, treatment failure and shorter survival. Germline variants in p53 signaling pathway genes could also lead to p53 dysfunction, but their involvement in CLL has not been thoroughly evaluated. The aim of this study was to determine the association of TP53, MDM2 and NQO1 gene variability with clinical and genetic data of CLL patients. Individual genotype and haplotype data of CLL patients were compared with clinical prognostic factors, cytogenetic and molecular cytogenetic findings as well as IGHV and TP53 mutational status. The study included 116 CLL patients and 161 healthy blood donors. TP53 (rs1042522, rs59758982, rs1625895), NQO1 (rs1800566) and MDM2 (rs2279744, rs150550023) variants were genotyped using different PCR approaches. Analysis of genotype frequencies revealed no association with the risk of CLL. TP53 rs1042522, rs1625895 and MDM2 rs2279744 variants were significantly associated with abnormal karyotype and the presence of del(17p). Similarly, these two TP53 variants were associated with TP53 disruption. Moreover, TP53 C-A-nondel and G-A-del haplotypes (rs1042522-rs1625895-rs59758982) were associated with an increased likelihood of carrying del(17p) and TP53 disruptions. MDM2 T-nondel haplotype (rs2279744-rs150550023) was found to be a low risk factor for del(17p) (OR = 0.32; CI: 0.12-0.82; p = 0.02) and TP53 disruptions (OR = 0.41; CI: 0.18-0.95; p = 0.04). Our findings suggest that TP53 and MDM2 variants may modulate the risk to have chromosome alterations and TP53 disruptions, particularly del(17p). To our knowledge this is the first study of several germline variants in p53 pathway genes in Argentine patients with CLL.

Efecto del Aprendizaje Basado en Problemas en habilidades de Gestión del Cuidado: Estudio cuasiexperimental / The effect of Problem-Based Learning on Care Management skills: A quasi-experimental study / O efeito da Aprendizagem Baseada em Problemas nas habilidades de Gestão do Cuidado: Estudo quase-experimental

Objetivo: evaluar el efecto preliminar del Aprendizaje Basado en Problemas en habilidades de Gestión del Cuidado. Método: cuasiexperimental pretest-postest, con alumnos de la carrera de Licenciatura en Enfermería de una institución educativa. Muestra de 29 alumnos (Grupo Experimental) y 74 (Grupo Control). El Grupo Experimental solucionó cuatro escenarios bajo el método de Aprendizaje Basado en Problemas con los 7 pasos propuestos por la Universidad de McMaster, en un programa de Gestión del Cuidado en modalidad a distancia. El instrumento autoinformado evaluó las habilidades de Gestión del Cuidado pretesty postesten ambos grupos. Se obtuvieron valores medios y se realizó estadística descriptiva e inferencial (t de Student, t pareada, regresión lineal). Resultados: el Grupo Experimental obtuvo puntuaciones más elevadas en habilidades analíticas, de acción y globales que el Grupo Control (p<0,05). No se registraron diferencias en las habilidades interpersonales ni en el uso de la información. El Grupo Control no presentó diferencias significativas antes y después de la enseñanza habitual, mientras que en el Grupo Experimental sí se reportaron diferencias (p<0,05). Conclusión: a pesar de que existe poca evidencia en el desarrollo de habilidades de Gestión del Cuidado en Enfermería, el presente estudio demuestra que el Aprendizaje Basado en Problemas es un método efectivo y significativo en educación a distancia.

Objective: to assess the preliminary effect of Problem-Based Learning on Care Management skills. Method: a quasi-experimental pre- and post-test conducted with students attending the Bachelor's Degree in Nursing offered by an educational institution. The sample was comprised by 29 (Experimental Group) and 74 (Control Group) students. The Experimental Group solved four scenarios under the Problem-Based Learning method with the 7 steps proposed by McMaster University, in a Care Management program in distance mode. The self-reporting instrument assessed the pre- and post-test Care Management skills in both groups. Mean values were obtained and descriptive and inferential statistics were performed (Student's t, paired t, linear regression). Results: the Experimental Group obtained higher scores in analytical, action-related and global skills than the Control Group (p<0.05). No differences were recorded in interpersonal skills or in use of the information. The Control presented no significant differences before and after usual teaching, whereas differences were in fact reported in the Experimental Group (p<0.05). Conclusion: despite the fact that there is little evidence on the development of Nursing Care Management skills, the current study shows that Problem-Based Learning is an effective and significant method in remote education.

Objetivo: avaliar o efeito preliminar da Aprendizagem Baseada em Problemas nas habilidades de Gestão do Cuidado. Método: pré e pós-teste quase experimental, realizado com alunos do curso de Bacharelado em Enfermagem de uma instituição de ensino. A amostra foi composta por 29 (Grupo Experimental) e 74 (Grupo Controle). O Grupo Experimental resolveu quatro cenários sob o método de Aprendizagem Baseada em Problemas com os 7 passos propostos pela McMaster University, em um programa de Gestão do Cuidado na modalidade à distância. O instrumento de autorrelato avaliou as habilidades de Gestão do Cuidado pré e pós-teste em ambos os grupos. Valores médios foram obtidos e estatísticas descritivas e inferenciais foram realizadas (t de Student, t pareado, regressão linear). Resultados: o Grupo Experimental obteve escores mais elevados em habilidades analíticas, de ação e globais do que o Grupo Controle (p<0,05). Não foram registradas diferenças nas habilidades interpessoais ou no uso da informação. O Grupo Controle não apresentou diferenças significativas antes e depois do ensino usual, enquanto as diferenças foram de fato relatadas no Grupo Experimental (p<0,05). Conclusão: apesar de haver poucas evidências sobre o desenvolvimento de habilidades de Gestão do Cuidado de Enfermagem, o presente estudo mostra que a Aprendizagem Baseada em Problemas é um método eficaz e significativo na educação à distância.

Pooling saliva samples as an excellent option to increase the surveillance for SARS-CoV-2 when re-opening community settings.

In many countries a second wave of infections caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has occurred, triggering a shortage of reagents needed for diagnosis and compromising the capacity of laboratory testing. There is an urgent need to develop methods to accelerate the diagnostic procedures. Pooling samples represents a strategy to overcome the shortage of reagents, since several samples can be tested using one reaction, significantly increasing the number and speed with which tests can be carried out. We have reported the feasibility to use a direct lysis procedure of saliva as source for RNA to SARS-CoV-2 genome detection by reverse transcription quantitative-PCR (RT-qPCR). Here, we show that the direct lysis of saliva pools, of either five or ten samples, does not compromise the detection of viral RNA. In addition, it is a sensitive, fast, and inexpensive method that can be used for massive screening, especially considering the proximity of the reincorporation of activities in universities, offices, and schools.

Family functioning and school connectivity in Mexican high school adolescents / Funcionamento familiar e conectividade escolar em adolescentes mexicanos do Ensino Médio

Family functionality has shown its relevance in the comprehensive development of adolescents, with school connectivity being a very important aspect. To determine the association between family functioning and school connectivity in Mexican high school adolescents, an analytical cross-sectional study was carried out in a census of 396 adolescents in a public high school in Mexico. The self-report of family functioning and the school connectedness scale were used, both with α > 0.84. ꭕ2 and ANOVA were applied. The results show significant differences in conflict with shift (morning ꭕ2 = 42.47 vs. evening ꭕ2 = 40.35, F = 4.57, p = 0.033), and leadership with degree (1st ꭕ2 = 8.14, 2nd ꭕ2 = 8.97, 3rd ꭕ2 = 8.90, F = 3.52, p = 0.030). Connectivity associated with school variables (p < 0.05). There are no differences or association between sex, age or qualifications with connectivity. Family functioning was associated in affective expression with school (ꭕ2 = 3.77, p = 0.055) and in leadership with students (ꭕ2 = 2.92, p = 0.05). It is concluded that the leadership of the parents and the affective expression of the family favors school connectivity between students and with their school.

A funcionalidade familiar tem mostrado sua relevância no desenvolvimento integral dos adolescentes, sendo a conectividade escolar um aspecto muito importante. Para determinar a associação entre funcionamento familiar e conectividade escolar em adolescentes mexicanos do Ensino Médio, foi realizado um estudo transversal analítico em um censo de 396 adolescentes em uma escola pública no México. Foram utilizados o autorrelato de funcionamento familiar e a escala de conectividade escolar, ambos com α > 0,84. ꭕ2 e ANOVA foram aplicados. Os resultados mostram diferenças significativas no conflito com turno (ꭕ2 da manhã = 42,47 vs. ꭕ2 da noite = 40,35, F = 4,57, p = 0,033) e liderança com grau (1º ꭕ2 = 8,14, 2º ꭕ2 = 8,97, 3º ꭕ2 = 8,90, F = 3,52, p = 0,030). Conectividade associada a variáveis escolares (p < 0,05). Não há diferenças ou associação entre sexo, idade ou qualificações com conectividade. O funcionamento familiar foi associado na expressão afetiva com a escola (ꭕ2 = 3,77, p = 0,055) e na liderança com os alunos (ꭕ2 = 2,92, p = 0,05). Conclui-se que a liderança dos pais e a expressão afetiva da família favorece a conectividade escolar entre os alunos e com sua escola.

An Extensive Quality Control and Quality Assurance (QC/QA) Program Significantly Improves Inter-Laboratory Concordance Rates of Flow-Cytometric Minimal Residual Disease Assessment in Acute Lymphoblastic Leukemia: An I-BFM-FLOW-Network Report.

Monitoring of minimal residual disease (MRD) by flow cytometry (FCM) is a powerful prognostic tool for predicting outcomes in acute lymphoblastic leukemia (ALL). To apply FCM-MRD in large, collaborative trials, dedicated laboratory staff must be educated to concordantly high levels of expertise and their performance quality should be continuously monitored. We sought to install a unique and comprehensive training and quality control (QC) program involving a large number of reference laboratories within the international Berlin-Frankfurt-Münster (I-BFM) consortium, in order to complement the standardization of the methodology with an educational component and persistent quality control measures. Our QC and quality assurance (QA) program is based on four major cornerstones: (i) a twinning maturation program, (ii) obligatory participation in external QA programs (spiked sample send around, United Kingdom National External Quality Assessment Service (UK NEQAS)), (iii) regular participation in list-mode-data (LMD) file ring trials (FCM data file send arounds), and (iv) surveys of independent data derived from trial results. We demonstrate that the training of laboratories using experienced twinning partners, along with continuous educational feedback significantly improves the performance of laboratories in detecting and quantifying MRD in pediatric ALL patients. Overall, our extensive education and quality control program improved inter-laboratory concordance rates of FCM-MRD assessments and ultimately led to a very high conformity of risk estimates in independent patient cohorts.

Similarities and Differences in Substance Use Patterns Among Lesbian, Gay, Bisexual, and Heterosexual Mexican Adult Smokers.

Purpose: This study aimed to characterize the patterns of smoking, e-cigarette use, other substance use (alcohol and marijuana), and depression by sexual orientation in a sample of Mexican adult smokers. Methods: Data came from a 2018-2020 (six waves) online survey of adult smokers, recruited from a commercial research panel (92.5% heterosexual, n = 4786; 3.1% lesbian/gay, n = 160; and 4.4% bisexual, n = 229). After stratifying the data by sex, logistic, multinomial, and linear logistic regression models were estimated (depending on the outcome), including as independent variables sexual orientation (i.e., gay/lesbian, bisexual, heterosexual = Reference), age, education, household income, and wave. Results: Being a gay male was independently associated with greater smoking dependence (ß = 0.20; 95% confidence interval [CI]: 0.02 to 0.39), greater likelihood of preference for flavored capsule cigarettes (adjusted odds ratio [AOR] = 2.10, 95% CI: 1.33 to 3.28), and depression diagnosis (AOR = 2.85, 95% CI: 1.64 to 4.95). Bisexual males had higher e-cigarette dependence (ß = 0.37; 95% CI: 0.05 to 0.68, among dual users only) and were more likely to have been diagnosed with depression (AOR = 2.34, 95% CI: 1.30 to 4.18). Lesbian females were more likely to prefer menthol cigarettes (AOR = 3.32, 95% CI: 1.60 to 6.86), to have used marijuana more than once (AOR = 3.23, 95% CI: 1.83 to 5.72), and to have depressive symptoms (AOR = 1.85, 95% CI: 1.04 to 3.29). Bisexual females had a greater likelihood of depressive symptoms (AOR = 1.71, 95% CI: 1.14 to 2.56) and depression diagnosis (AOR = 2.22, 95% CI: 1.43 to 3.42). Conclusion: Lesbian, gay, and bisexual adult smokers in Mexico appear more likely than heterosexual adult smokers to report having depression. Substance use and depression among sexual minority populations need to be addressed further.

Funcionalidad familiar y rendimiento académico en estudiantes de ciencias de la salud: una revisión sistemática / Family functionality and academic performance in health sciences students: a systematic review

RESUMEN Objetivo: Identificar los factores de la funcionalidad familiar asociados al rendimiento académico de los estudiantes de ciencias de la salud a nivel de educación superior, según lo reportado en la literatura. Materiales y método: Se llevó a cabo una revisión sistemática de la literatura en las siguientes bases de datos: PubMed (MedLine), ProQuest, SciELO y Google Académico, utilizando descriptores clave, criterios de selección y límites de búsqueda durante el proceso de revisión. Veintitrés documentos fue el insumo final seleccionado para el análisis. Resultados: Adicional a los hallazgos descriptivos de los estudios, emergieron factores positivos y negativos que se constituyen en categorías explicativas de la asociación existente entre la funcionalidad familiar y el rendimiento académico en los estudiantes de ciencias de la salud en educación superior. Conclusión: La literatura científica sobre funcionalidad familiar y su asociación con el rendimiento académico en los estudiantes universitarios, aunque ha ido en aumento de forma significativa, se ha desarrollado desde perspectivas diversas. Se requiere mayor integración y estudios de investigación que incluyan a la familia y a la universidad como entornos próximos y de desarrollo de los estudiantes.

ABSTRACT Objective: to identify the factors of family functionality, associated with the academic performance of health sciences students at the higher education level, as reported in the literature. Materials and Method: a systematic review of the literature was carried out in the following databases: PubMed (MedLine), ProQuest, SciELO, and Google Scholar, using key descriptors, selection criteria, and search limits during the review process. 23 documents were selected as the final input for the analysis. Results: in addition to the descriptive findings of the studies, positive and negative factors emerged that constitute explanatory categories of the existing association between family functionality and academic performance in health sciences students in higher education. Conclusion: the scientific literature on family functionality and its association with academic performance in university students, although has been significantly increasing, has developed from different perspectives. Greater integration and research studies that include the family and the university as close and developmental environments for students are required.

Hábitos de alimentación asociados a sobrepeso y obesidad en adultos mexicanos: una revisión integrativa

RESUMEN Objetivo: Describir hallazgos publicados de hábitos de alimentación y su relación con sobrepeso y obesidad en adultos mexicanos. Material y Método: Revisión integrativa basada en 5 pasos sugeridos por Crossetti, utilizando las palabras clave "hábitos de alimentación", "sobrepeso", "obesidad" y "adultos mexicanos", con operadores booleanos "AND" y "OR"; se buscó en Scielo, PubMed, Conricyt, Scopus y WOS, entre los años 2005 y 2020, publicaciones en español e inglés. Resultados: Se identificaron 64 artículos, de estos se descartaron 52, quedando 12 acordes a criterios de inclusión; los artículos fueron analizados en extenso, identificando 4 categorías: 1) Zona de vivienda; 2) Estrato socioeconómico; 3) Género y 4) Cultura; cada una de ellas con subcategorías. Conclusiones: Las zonas rurales reportan mayor consumo de frutas, verduras, cereales, vitaminas y menor consumo calórico; en zonas urbanas se consume mayor cantidad de grasa, alimentos procesados, azúcares refinadas y menos vegetales, frutas y cereales. Un mayor estrato socioeconómico se asocia con alimentación menos calórica y comidas más naturales. Los hombres consumen más carne, grasa y alcohol, las mujeres consumen más hidratos de carbono y azúcares refinadas. En cuestiones culturales reportan distracciones a la hora de comer, como uso de celular, bajo consumo de agua y alto consumo de gaseosas (70%); refieren barreras para alimentarse sanamente, como el trabajo pagado y doméstico, realizando comidas a prisa, sin considerar la calidad de los alimentos que se consumen. Estos hallazgos sugieren considerar factores socioeconómicos y culturales en las acciones preventivas que los profesionales de enfermería realizan en la práctica clínica y comunitaria.

ABSTRACT Objective: To describe published findings on eating habits and their relationship with overweight and obesity in Mexican adults. Materials and Methods: Integrative review based on 5 steps suggested by Crossetti, using the keywords "feeding habits", "overweight", "obesity" and "Mexican adults" along with Boolean operators "AND" and "OR" in the Scielo, PubMed, Conricyt, Scopus and WOS databases, with publications in Spanish and English, between the years 2005 and 2020. Results: 64 articles were identified and 52 were discarded, leaving 12 that complied with the inclusion criteria. The articles were extensively analyzed, identifying 4 categories: 1) Housing area; 2) Socioeconomic stratum; 3) Gender and 4) Culture; each of them divided into subcategories. Conclusions: Rural areas report higher consumption of fruits, vegetables, cereals, vitamins and lower caloric consumption, while in urban areas more fat, processed food, refined sugars and fewer vegetables, fruits and cereals are consumed. A higher socioeconomic stratum is associated with a less caloric diet and more natural food. Men consume more meat, fat and alcohol, while women consume more carbohydrates and refined sugars. Regarding cultural issues, people report distractions at mealtime, such as cell phone use, low water consumption and high consumption of soft drinks (70%); they also refer to barriers to healthy eating, such as work, eating meals quickly without considering the quality of food. These findings suggest considering socioeconomic and cultural factors in the preventive actions that nursing professionals carry out in clinical and community practice.

RESUMO Objetivo: Descrever os resultados publicados sobre hábitos alimentares e sua relação com sobrepeso e obesidade em adultos mexicanos. Material e Método: Revisão integrativa baseada em 5 etapas sugeridas por Crossetti, utilizando as palavras-chave "hábitos alimentares", "sobrepeso", "obesidade" e "adultos mexicanos", com operadores booleanos "AND" e "OR", a busca foi realizada em Scielo, PubMed, Conricyt, Scopus e WOS, entre os anos de 2005 e 2020, publicações em espanhol e inglês. Resultados: Foram identificados 64 artigos, destes, 52 foram descartados, restando 12, de acordo com os critérios de inclusão; os artigos foram amplamente analisados, identificando 4 categorias: 1) Área de habitação; 2) Estrato socioeconómico; 3) Gênero e, 4) Cultura; cada um deles com subcategorias. Conclusões: As áreas rurais relatam maior consumo de frutas, hortaliças, cereais, vitaminas e menor consumo calórico; nas áreas urbanas, o consumo maior é de gordura, alimentos processados, açúcares refinados e menor consumo de vegetais, frutas e cereais. Um estrato socioeconómico mais elevado está associado a uma dieta menos calórica e alimentos mais naturais. Os homens consomem mais carne, gordura e álcool, as mulheres consomem mais carboidratos e açúcares refinados. Em questões culturais, relatam distrações na hora das refeições, como uso do celular, baixo consumo de água e alto consumo de refrigerantes (70%), referem-se a barreiras para uma alimentação saudável, como trabalho remunerado e doméstico, alimentação rápida, sem considerar a qualidade dos alimentos consumidos. Os resultados sugerem considerar fatores socioeconómicos e culturais nas ações preventivas que os profissionais de enfermagem realizam na prática clínica e comunitária.

Increased Expression of Autophagy Protein LC3 in Two Patients With Progressing Chronic Lymphocytic Leukemia.

Chronic lymphocytic leukemia (CLL) is the most common type of adult leukemia in the western hemisphere. It is characterized by a clonal proliferation of a population of CD5+ B lymphocytes that accumulate in the secondary lymphoid tissues, bone marrow, and blood. Some CLL patients remain free of symptoms for decades, whereas others rapidly become symptomatic or develop high-risk disease. Studying autophagy, which may modulate key protein expression and cell survival, may be important to the search for novel prognostic factors and molecules. Here, we applied flow cytometry technology to simultaneously detect autophagy protein LC3B with classical phenotypical markers used for the identification of tumoral CLL B cell clones. We found that two patients with progressing CLL showed increased expression of the autophagy protein LC3B, in addition to positive expression of CD38 and ZAP70 and unmutated status of IGHV. Our data suggest that activation of autophagy flux may correlate with CLL progression even before Ibrutinib treatment.

Efecto de la Socialización Organizacional en el Engagement de Trabajadores Mexicanos: Claridad y Futuro / Effect of Organizational Socialization on Mexican workers' engagement: Clarity and Future

Resumen Introducción: En el estudio del comportamiento organizacional se destaca con más frecuencia la importancia del proceso de socialización organizacional, relacionado con la adaptación al trabajo y el desarrollo de las competencias para el rol laboral; así como un interés creciente por los estados positivos en el trabajo como el work engagement (caracterizado por el vigor, dedicación y absorción) y que puede evidenciar la identificación del trabajador con la cultura de la organización. Objetivo: Examinar el efecto de los dominios de la socialización organizacional sobre el work engagement que reportan tener los trabajadores mexicanos de la muestra. Método: Estudio transversal-correlacional analítico, mediante análisis de regresión con los datos del Inventario de Socialización Organizacional de Taormina (1994), y el Utrecht Work Engagement Scale, en su versión al español por Schaufeli, Bakker y Salanova (2006), en 187 trabajadores mexicanos. Resultados: Los análisis de regresión mostraron que la combinación de la comprensión funcional y las perspectivas de futuro se combinan para la aparición del vigor (Beta .29, .21), la absorción (Beta .23, .23) y la dedicación al trabajo (Beta .34, .25) en los trabajadores de la muestra. Se encontró que los cuatro factores de la socialización organizacional correlacionan con el vigor (.35), la absorción (.34) y la dedicación (.47). Conclusiones: En los trabajadores de la muestra la comprensión y las perspectivas de beneficios, generan efectos en el vigor, la absorción y la dedicación al trabajo. Se señala la importancia de la socialización en la aparición de engagement como antecedente y componente de la productividad y el bienestar en el trabajo.

Abstract Introduction: not only the importance of the process of organizational socialization related to work adaptability and the development of competencies for the job role, has recently been highlighted more frequently in organizational behavior studies, but also a growing interest in positive states of work such as work engagement (characterized by vigor, dedication and absorption) and which can demonstrate the identification of the worker with the culture of the organization. Objective: to examine the effect of the domains of organizational socialization on the work engagement reported by the Mexican workers in the sample. Method: A transversal-correlational analytical study through a regression analysis with the data of the Inventory of Organizational Socialization of Taormina (1994), and the Utrecht Work Engagement Scale, in its Spanish version by Wilmar Schaufeli, Arnold Bakker and Salanova (2006), in 187 Mexican workers, was conducted. Results: Regression analysis showed that the combination of understanding and future prospects were integrated for the display of vigor (Beta .29, .21), absorption (Beta .23, .23) and dedication to work (Beta .34, .25) in the sample workers. It was found that the four factors of organizational socialization correlate with vigor (.35), absorption (.34) and dedication (.47).dedication. Conclusions: In the workers of the sample the understanding and the perspectives of benefits, generate effects in the vigor, the absorption and the dedication to the work. The importance of socialization in the appearance of engagement as a psychological state related to productivity and well-being at work is pointed out.

Tumor-stroma interaction increases CD147 expression in neoplastic B lymphocytes in chronic lymphocytic leukemia.

BACKGROUND: Chronic lymphocytic leukemia (CLL) microenvironment plays a critical role in disease pathogenesis. Matrix metalloproteinases (MMPs) are involved in CLL-B cell migration and survival. CD147 is associated with MMPs production by tumor and stromal cells. AIM: To analyze CD147, MMP2 and MMP9 expression in CLL-B cells and its modulation by fibroblasts (Fb)-CLL-B cell interaction. METHODS: CLL-B cells were co-cultured with Fb, as a simulation of CLL microenvironment. CD147 was evaluated in healthy donor (HD)-B cells and CLL-B cells by flow cytometry. MMP2 and MMP9 activity in CLL-plasma samples and conditioned media (CMs) was studied by zymography. RESULTS: MMP9/MMP2 plasma levels were significantly higher in CLL patients than in HD. CD147 expression (median fluorescence intensity) in CLL patients characterized 3 groups: low- (19.1 ± 3.2; n=3), middle- (42.7 ± 12.8; n=18) and high- (76.5 ± 9.6; n=5) related to CD147 expression in HD-B cells. CD147 expression significantly increased in CLL-B cells after Fb-CLL-B cell co-culture. A significant increase in proMMP2 activity was observed in CMs obtained from Fb-CLL-B cell co-cultures in comparison with isolated CLL-B cells. CONCLUSIONS: CD147 expression in CLL-B cells and MMPs secretion was induced by Fb-CLL-B cell contact, suggesting CD147 participation in the CLL pathophysiology.

External validation of the PUMA COPD diagnostic questionnaire in a general practice sample and the PLATINO study population.

Background: A seven-item prescreening questionnaire (gender, age, pack-years smoking, dyspnea, sputum, cough, previous spirometry data) was developed for COPD detection in the primary care setting (PUMA Study) of four Latin America countries. Objectives: To validate the PUMA prescreening COPD questionnaire externally in two different populations (primary care and general). Methods: The PUMA prescreening COPD questionnaire score was applied to subjects from the Hospital Maciel, Montevideo (primary care), case-finding program and the PLATINO population (general) using PUMA study inclusion criteria. Post-bronchodilator FEV1/FVC <0.70 and lower limit of normal (LLN) criteria were used to define COPD. Area under the received operator curve (ROCAUC), sensitivity, specificity, predictive positive and negative values (PNV), number needed to treat (NNT), and best cut-points of the score were calculated. Results: 974 individuals from Hospital Maciel and 2512 from the PLATINO population were eligible, using post-bronchodilator FEV1/FVC <0.70, 45.1% and 18.7% had COPD, respectively, and using LLN 38.4% and 15.4% had COPD, respectively. From Hospital Maciel (post-bronchodilator FEV1/FVC <0.70), the best cut-point of ≥6 had moderate discriminatory power (ROCAUC 0.70), sensitivity 69.9%, specificity 62.1%, PNV 70.9%, and NNT of 3. The discriminatory power was 0.73 (ROCAUC) in the PLATINO population with three potential cut-points (Youden's index): ≥3 (sensitivity 85.4%, specificity 46.9%), ≥4 (sensitivity 66.7%, specificity 66.5%), and ≥5 (sensitivity 51.5%, specificity 81.6%); the PNV at each cut-point was 93.3%, 89.9%, and 88.0%, respectively. The NNT was 5 for scores ≥3 and ≥4, and 4 for ≥5. The mean accuracy using the LLN for Hospital Maciel and PLATINO was 0.67 and 0.70, respectively. Conclusion: External validation of the PUMA prescreening questionnaire in two Latin American populations (general and primary care) suggests moderate accuracy, similar to the original study in which the questionnaire was developed.

Descripción de actitudes sobre ética profesional en estudiantes de dos escuelas profesionales en el área de salud, 2016 / Description of attitudes about professional ethics in students of two professional schools of health, 2016

Introducción: La universidad tiene la responsabilidad de formar profesionales integrales comprometidos éticamente en su actuar profesional. Objetivo: Comparar las actitudes sobre ética profesional que tienen los estudiantes de dos escuelas del área de salud de la Universidad Nacional Mayor de San Marcos. Método: Estudio transversal. Participaron del estudio 284 estudiantes a los que se les aplicó la Escala de Actitudes sobre Ética Profesional de Hirsch, adecuado y validado para estudiantes de pregrado, en cuatro competencias (cognitivas y técnicas, sociales, éticas, afectivo-emocionales). Resultados: La valoración de las respuestas a las cuatro competencias fue similar en ambas escuelas, siendo las positivas mayores a 80% en tres competencias. Conclusión: En ambos grupos se encontró mayor porcentaje de actitud positiva ante las cuatro competencias, valorando en primer lugar las afectivo-emocionales, segundo lugar las cognitivas y técnicas, tercer lugar las éticas y por último las sociales.

Introduction: The university has the responsibility to train integral professionals, who are ethically committed in their professional actions. Objective: To compare the attitudes about professional ethics in students of two schools of health area from the Universidad Nacional Mayor de San Marcos. Methods: A transversal study. 284 undergraduate students participated in the study and a validate version of Hirsch Professional Ethics Attitude Scale was applied to them. Four competences from Hirch’s scale were evaluated: cognitive and technical, social, ethical and affective-emotional. Results: The assessment of the answers was similar in both schools, with positive ones greater than 80% in three competitions. Conclusion: A higher percentage of positive attitude was found in the four competences. Both groups valuing in first place, the affective-emotional component; in second place, cognitive and technical component; thirdly, ethics and finally, the social component.

Validación del perfil de estrés de nowack en estudiantes universitarios mexicanos / Validation of nowack's stress profile in mexican university students

RESUMEN Objetivos a. Adaptar y validar el "Perfil de Estrés" para la población mexicana; b. Determinar la proporción de varianza que el instrumento es capaz de explicar de los datos originales a partir de un modelo factorial; c. Agrupar las variables en los factores en donde saturan con mayor claridad; d. Evaluar la validez de contenido del instrumento, y; e. Evaluar la consistencia interna del instrumento. Método La muestra de validación estuvo compuesta por 883 participantes, 58.3% mujeres y 41.7% hombres, con edades entre 15 y 76 años (M=21.40, DE=10.02). Para verificar la validez psicométrica de la escala se llevaron a cabo el procedimiento propuesto por Reyes-Lagunes y García-Barragán (2008), el cual consta de los siguientes pasos: a. Frecuencia incluyendo sesgo para la selección de reactivos; b. Discriminación de reactivos para grupos extremos con base en el cual se eliminarán los que no cumplen con el requisito; c. Confiabilidad interna, y; d. Validez. Resultados El "Perfil de Estrés" presenta características psicométricas adecuadas para la población mexicana (alfa de Cronbach de 0.65 a 0.93). Conclusión El "Perfil de estrés" fue adaptado y validado para la cultura mexicana. Se considera que se trata de un instrumento útil para estudios que se propongan evaluar los factores asociados con el estrés en general, incluyendo los hábitos de salud, los estilos de afrontamiento y bienestar psicológico.(AU)

ABSTRACT Objectives This work sees to a) adapt and validate the "Stress profile" for the Mexican population, b) determine the proportion of variance that the instrument is able to explain based on the original data of a factorial model, c) group the variables into factors where they saturate most clearly, d) evaluate the validity of the content of the instrument, and e) evaluate the internal consistency of the instrument. Methods The validation sample consisted of 883 participants, 58.3% women and 41.7% men, aged between 15 and 76 years (M=21.40, SD=10.02). To verify the psychometric validity of the scale, the procedure proposed by Reyes-Lagunes and García-Barragán (2008) was used. It consists of the following steps: a) frequency, including bias for the selection of reagents; b) discrimination of reagents for extreme groups, having in mind that those that do not comply with the requirement will be eliminated; c) internal reliability; and d) validity. Results The "Stress Profile" has adequate psychometric characteristics for the Mexican population (Cronbach's alpha of 0.65 to 0.93). Conclusion The "Stress Profile" was adapted and validated for the Mexican population. This is a useful tool for studies that aim to evaluate the factors associated with stress in general, including health habits, coping styles and psychological well-being.(AU)

Impairment of functioning and substance use in a Latino population.

This study investigated the association of academic outcomes, romantic relationships, and substance use (tobacco, marijuana, cocaine) with alcohol dependence in a sample of Latino (N = 1,143) college students. Secondary data analysis was conducted on measures of grade point average in college, relationship satisfaction, drug use, and alcohol dependence. Latino college students who reported alcohol dependency had significant relational dissatisfaction and poor academic outcomes. Thus, lower grade point average and relationship dissatisfaction were associated with alcohol dependence. By focusing specifically on a Mexican American population, this study adds important information to current research regarding the commonality and differences across cultural groups regarding drug use and dependence and further clarifies the risk factors associated with substance use and dependency in a population that is vulnerable for at-risk behaviors. This study also offers insight into potential targets of treatment and intervention for this cultural group.

Usos y actitudes del personal de enfermería acerca de las terapias alternativas en un hospital pediátrico / Uses and attitudes of the nursing staff about alternative therapies in a pediatric hospital

Resumen

Introducción: en México el uso de las terapias alternativas es popular. El personal de enfermería es por excelencia el educador del paciente y la familia; por lo tanto, sus actitudes y opiniones son relevantes acerca del uso de las TA.

Objetivo: conocer el uso de las terapias alternativas que hace el personal de enfermería y su opinión y actitudes en torno a ese uso con los pacientes y la familia.

Metodología: estudio descriptivo transversal, mediante el cual se le aplicaron 268 cuestionarios al personal de enfermería. El cuestionario fue diseñado ex profeso y validado para su aplicación.

Resultados: el 68% utilizó terapias alternativas y la mitad consideró que son seguras, el 27% mencionó que las recomienda a los pacientes y el 5% tuvo conocimiento del tema. El uso personal se asoció con la recomendación.

Conclusiones: la mayoría no indaga el antecedente del uso de terapias alternativas que hacen los pacientes. La recomendación de su uso se basa probablemente en la experiencia personal.

Abstract

Introduction: In Mexico the use of alternative therapies is popular. Par excellence, the nursing staff educates the patient and the family members; therefore, nursing staffs attitudes and opinions are relevant about the use of these therapies.

Objective: To know the use of alternative therapies by the nursing staff and their opinion and attitudes with patients and family concerning this use.

Methods: Cross-sectional descriptive study. 268 questionnaires were administered to the nursing staff. The questionnaire was designed with a purpose and validated.

Results: 68% of the nurse staff used alternative therapies, half believed these therapies are safe, 27% recommended them to patients, and 5% had knowledge of the subject. Personal use was associated with the recommendation.

Conclusions: The majority of the nursing staff does not investigate the antecedent of the use of alternative therapies by patients. The recommendation of its use is probably based on personal experience.