The inhibitor of the redox activity of APE1/REF-1, APX2009, reduces the malignant phenotype of breast cancer cells.

Apurinic/apyrimidinic endonuclease 1/redox factor-1 (APE1/REF-1) is a multifunctional protein acting on cellular signaling pathways, including DNA repair and redox activities. APE1/REF-1 has emerged as a target for cancer therapy, and its role in breast cancer models would reveal new strategies for cancer therapy. APX2009 is a specific APE1/REF-1 redox inhibitor whose anticancer properties have not been described in breast cancer cells. Here, we investigated the effect of the APX2009 treatment in the breast cancer cell lines MDA-MB-231 and MCF-7. Breast cancer cell lines were cultured, and WST1 and colony formation assays were performed to evaluate cell proliferation. Annexin V-FITC/7-AAD and LDH-Glo™ assays were performed to evaluate cell death. The wound healing assay and Matrigel transwell assay were performed after APX2009 treatment to evaluate the cellular migration and invasion processes, respectively. Our findings demonstrated that APX2009 treatment decreased breast cancer cell proliferative, migratory, and invasive properties. Furthermore, it induced apoptosis in both cell lines. Our study is the first to show the effects of APX2009 treatment on apoptosis in a breast cancer cell. Therefore, this study suggested that APX2009 treatment is a promising anticancer molecule for breast cancer.

The inhibitor of the redox activity of APE1/REF-1, APX2009, reduces the malignant phenotype of breast cancer cells

Apurinic/apyrimidinic endonuclease 1/redox factor-1 (APE1/REF-1) is a multifunctional protein acting on cellular signaling pathways, including DNA repair and redox activities. APE1/REF-1 has emerged as a target for cancer therapy, and its role in breast cancer models would reveal new strategies for cancer therapy. APX2009 is a specific APE1/REF-1 redox inhibitor whose anticancer properties have not been described in breast cancer cells. Here, we investigated the effect of the APX2009 treatment in the breast cancer cell lines MDA-MB-231 and MCF-7. Breast cancer cell lines were cultured, and WST1 and colony formation assays were performed to evaluate cell proliferation. Annexin V-FITC/7-AAD and LDH-Glo™ assays were performed to evaluate cell death. The wound healing assay and Matrigel transwell assay were performed after APX2009 treatment to evaluate the cellular migration and invasion processes, respectively. Our findings demonstrated that APX2009 treatment decreased breast cancer cell proliferative, migratory, and invasive properties. Furthermore, it induced apoptosis in both cell lines. Our study is the first to show the effects of APX2009 treatment on apoptosis in a breast cancer cell. Therefore, this study suggested that APX2009 treatment is a promising anticancer molecule for breast cancer.

Prevalence and distribution of dental anomalies in a paediatric population based on panoramic radiographs analysis.

AIM: To evaluate the frequency and distribution of dental anomalies (DA) in a paediatric population. MATERIALS AND METHODS: Panoramic digital radiographs of children between 6 and 12 years old performed at a reference centre for radiographic exams were accessed. Two calibrated examiners evaluated the radiographs. The association between variables and outcomes was assessed using non-parametric tests. The significance level was set at 5%. RESULTS: Five hundred and twelve individuals were included and 61.3% presented at least one DA. The mean age of patients with DA (9.35±1.60) was significantly higher compared to patients with no anomalies (7.90±1.58). Patients with supernumerary tooth, however, were significantly younger. The most frequent DA were permanent tooth radicular dilacerations (38.1%), permanent tooth agenesis (29.3%), supernumerary tooth (6.4%) and impacted tooth (6.4%). Anterior teeth and female patients were more affected by radicular dilacerations. Dental agenesis was more frequent in the third molars followed by the upper lateral incisor. CONCLUSION: DA are quite frequent in the paediatric population and the most common DA observed herein could only be identified through imaging exams. The most appropriate timing to perform an investigation for the detection of dental anomalies appears to be between 9 and 10 years old.

Determination of malondialdehyde, acrolein and four other products of lipid peroxidation in edible oils by Gas-Diffusion Microextraction combined with Dispersive Liquid-Liquid Microextraction.

A new and sensitive analytical method for the simultaneous determination of secondary lipid peroxidation aldehydes has been successfully developed and validated. Malondialdehyde, acrolein, formaldehyde, acetaldehyde, propanal, and pentanal were extracted and derivatized using 2,4-dinitrophenylhydrazine (DNPH) by gas-diffusion microextraction (GDME) combined with dispersive liquid-liquid microextraction (DLLME) for gas chromatography-mass spectrometry (GC-MS) analysis. The experimental conditions have been optimized by experimental designs. The analytical method validation, in accordance to the Food and Drug Administration (FDA) guidance, provided good results in terms of linearity with r2≥0.9974, in the range from 0.15 or 0.3 µg·g-1 to 3 µg·g-1. Limits of detection and limits of quantification were 0.05 or 0.10 and 0.15 or 0.3 µg·g-1, respectively. Precision was tested as a relative standard deviation (RSD≤ 9.5%) and recoveries were between 95% and 110%. The method was applied in the characterization of aldehydes in forty-eight edible oil samples; with the highest concentration found in pomace olive oil for malondialdehyde at 6.64 µg·g-1.

Occurrence and exposure of 3-monochloropropanediol diesters in edible oils and oil-based foodstuffs from the Spanish market.

During the industrial refining process of edible oils and the manufacture of oil-based foodstuff, contaminants such as 3-monochloropropanediol (3-MCPD) fatty acid diesters can be produced. One hundred samples of different edible oils and related fatty food purchased from local Spanish markets were analyzed to evaluate the occurrence of these contaminants. Data of seven 3-MCPD diesters together with corresponding total 3-MCPD equivalents are presented. The procedure is based on a modified QuEChERS protocol followed by LC-MS/MS analysis. Extra virgin olive oil (EVOO) and unrefined oils did not contain detectable levels of the target analytes. The highest levels of 3-MCPD diesters were found in palm oils, for 1,2-Dilinoleoyl-3-chloropropanediol (LILI) and 1-2-Bispalmitoyl-3-chloropropanediol (PAPA) with concentrations close to 10 mg kg-1 and in the lipid fraction of margarines (8.09, 3.77 and 3.72 mg kg-1 for LILI, PAPA and 1-Oleoyl-2-linoleoyl-3-chloropropanediol (OLLI), respectively).

Development of a partitioned liquid-liquid extraction- dispersive solid phase extraction procedure followed by liquid chromatography-tandem mass spectrometry for analysis of 3-monochloropropane-1,2-diol diesters in edible oils.

A fast and effective method using a modified QuEChERS (Quick, Easy, Cheap, Effective, Rugged and Safe) approach which includes partitioned liquid-liquid extraction (PLLE) and dispersive solid phase extraction (dSPE) clean-up step for the determination of seven 3-monochloropropane-1,2-diol (3-MCPD) fatty acid diesters in vegetable oils is developed and validated according to the Food and Drug Administration (FDA) guidelines. Due to the complexity of the matrices, combination of silica based sorbents (Silica Strong Anion Exchange (Si-SAX), Supel™ QuE Z-Sep+ (Z-Sep+) and Primary Secondary Amine (PSA) were tested for lipid removal. The effect of several experimental factors on the efficiency of the extraction procedure was studied by a screening design 3422//16 and a response surface Doehlert design. The separation and determination was carried out by liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS). The method provided suitable linearity (r2 > 0.9960), precision (relative standard deviation (RSD) lower than 10%) and accuracy, in terms of recovery. The limits of detection (LOD) and limits of quantification (LOQ) ranged from 10 to 20 µg kg -1 and from 25 to 50 µg kg-1, respectively. The recoveries at three spiking levels of 100, 250, and 500 µg kg-1 were over the range of 71.4-122.9% with RSD lower than 13%. The method was successfully applied in edible oils and fatty food samples. The results provide valuable information to assess the risk of exposure to these foodborne contaminants.

Energy partitioning and methane emission by sheep fed sorghum silages at different maturation stages / Partição de energia e emissão de metano por ovinos alimentados com silagem de sorgo em diferentes estádios de maturação

Energy partitioning and methane production by sheep fed silages of three commercially available sorghum hybrids (BRS 610, BR 700 and BRS 655) harvested at three maturation stages (milk, soft dough and floury) were evaluated in open circuit respiration chambers. A complete randomized design was used in a 3 × 3 (hybrids × maturity stages) factorial arrangement, and the means were compared by the Student-Newman-Keuls (SNK) test (P<0.05). The intake of dry matter, digestible dry matter, gross energy, digestible energy and metabolizable energy were not affected by maturation stage, but were influenced by hybrid. The net energy intake was influenced by maturity and sorghum genetics. The fecal output represented the main source of energy loss, as percentage of gross energy intake (48% to 52%), followed by heat increment (10% to 19%), methane emissions (4% to 6%) and urine (1% to 2%). There were no differences (P>0.10) among the treatments for the apparent digestibility of gross energy and metabolizability (qm). An interaction (P<0.05) between sorghum hybrid and maturation stages was observed for the efficiency of metabolizable energy utilization for maintenance (km), which ranged between 0.53 and 0.78. No differences (P>0.10) among treatments occurred in the daily methane production. There is substantial genetic diversity within sorghum species, determining different nutritional values. Sorghum genetics and maturity at harvest should not be an opportunity to reduce the contribution of agriculture to methane emissions.

A partição de energia e a produção de metano por ovinos alimentados com silagens de três híbridos de sorgo comerciais (BRS 610, BR 700 e BRS 655), colhidos em três estádios de maturação (leitoso, pastoso e farináceo), foram avaliadas em câmaras respirométricas de circuito aberto. Utilizou-se delineamento inteiramente ao acaso, em arranjo fatorial 3 x 3 (híbridos x estádios de maturação), e as médias foram comparadas pelo teste Student-Newman-Keuls (SNK) (P<0,05). Os consumos de matéria seca, de matéria seca digestível, de energia bruta, de energia digestível e de energia metabolizável não foram afetados pelo estádio de maturação, mas foram influenciados pelo híbrido de sorgo. O consumo de energia líquida foi influenciado pela maturidade e pelo híbrido. As perdas de energia nas fezes representaram a principal fonte de perda energética como porcentagem do consumo de energia bruta (48% a 52%), seguida pelo incremento calórico (10% a 19%), pela emissão de metano (4% a 6%) e pela urina (1 a 2%). Não houve diferenças (P>0,10) entre os tratamentos para a digestibilidade aparente da energia bruta e para a metabolizabilidade (qm). Foi observada interação (P<0,05) entre híbrido de sorgo e estádio de maturação para eficiência de uso da energia metabolizável para mantença (Km), que variou entre 0,53 e 0,78. Não ocorreram diferenças (P>0,10) entre tratamentos na produção diária de metano. A emissão de metano em gramas por kg de matéria seca digestível e por kg de fibra em detergente neutro digestível foi inferior no estádio pastoso comparado ao estádio farináceo.

Cinética de fermentação ruminal das silagens de três híbridos de milho comerciais em diferentes estádios de maturação / Ruminal fermentation kinetics of corn silages from three commercial hybrids at different maturation stages

Foi avaliada a cinética de fermentação ruminal in vitro de três híbridos comerciais de milho (BRS 1035, BRS 1031 e BRS 1001) colhidos em três estádios de maturidade (pastoso, farináceo e farináceo-duro). Após 96 horas de fermentação, não houve diferença na PCG entre as silagens dos híbridos colhidos nos diferentes estádios de maturidade (P>0,05). O potencial máximo de produção de gases das silagens de todos os híbridos reduziu com o avançar da maturidade da planta, sendo a variação encontrada de 259,3 a 280,0mL/g de matéria seca (MS). Os valores de tempo de colonização variaram de 0h06min a 1h54min. Para as silagens dos híbridos BRS 1035 e BRS 1001, houve aumento no tempo de colonização com o avançar da maturidade da planta. Para as silagens do híbrido BRS 1031, houve aumento dos valores de tempo de colonização quando a planta avançou do estádio pastoso para farináceo, e reduziu quando a maturidade alcançou o estádio farináceo-duro. A taxa fracional de produção de gases "µ" variou entre 0,03mL/h a 0,05mL/h. Com o avançar da maturidade fisiológica, as silagens dos híbridos BRS 1035 e BRS 1001 apresentaram aumento nos valores de µ; já para as silagens do híbrido BRS 1031, os valores de µ aumentaram quando o estádio de maturidade avançou de pastoso para farináceo, e reduziu para farináceo-duro. Os valores das degradabildades efetivas foram superiores para as silagens dos diferentes híbridos colhidas no estádio pastoso. As degradabilidades da MS com o tempo de fermentação de 96 horas variaram de 70,1 a 76,4%. A silagem do híbrido BRS 1035 apresentou melhor valor nutricional quando colhida no estádio pastoso de maturidade. Já a silagem do híbrido BRS 1031 apresentou valor nutricional superior no estádio farináceo, enquanto a silagem do híbrido BRS 1001 apresentou melhor valor nutricional no estádio farináceo-duro.

The in vitro ruminal fermentation kinetics of three commercial corn hybrids (BRS 1035, BRS 1031 and BRS 1001) harvested at three maturity stages (soft dough, floury and hard-floury) were evaluated. After 96 hours of fermentation there was no change in the cumulative gas production (GPC) among silages harvested for all hybrids (P>0.05). The silages maximal degradation potential for all hybrids decreased with advanced maturity stages, and ranged from 259.32 to 279.96mL/g of dry matter. The lag values ranged from 0h 06min to 1h 54min. For silages from the BRS 1035 and BRS 1001 hybrids, there was an increase in lag with advancing plant maturity. For silage from the BRS 1031 hybrid, the lag values increase when the plant moved of soft dough stage to floury, and was reduced to hard-floury stage. The fractional rate of gas production µ ranged from 0.0292mL/h to 0.0447mL/h. With advancing physiological maturity stages, the hybrids BRS 1035 and BRS 1001 showed an increase in the µ values, since for silage from the BRS 1031 hybrid, the value of µ increased when the maturity stage progressed from soft dough to floury, and reduced to hard-floury. The values of effective degradability were higher for silages from different hybrids harvested at soft dough. The dry matter degradability (DMD) in the fermentation time of 96 hours ranged from 70.14 to 76.40%. The silage from the BRS 1035 hybrid showed better nutritional value when harvested in the soft dough stage of maturity. The silage from the BRS 1031 hybrid showed highest nutritional value at the floury stage, while the silage from the BRS 1001 hybrid showed better nutritional value in the hard-floury stage.

Degradabilidade ruminal in situ do sorgo grão em diferentes formas de reconstituição / In situ ruminal degradability of sorghum grain in different forms of reconstruction

Foram utilizadas três vacas não lactantes fistuladas no rúmen com o objetivo de determinar os parâmetros de degradação ruminal in situ da matéria seca (MS) e do amido de grãos de sorgo sob diferentes formas de processamento: 1) Seco e moído (2mm); 2) Seco, moído (3mm), reidratado com aspersão de água sobre o material, e imediatamente ensilado por 30 dias; 3) Seco, inteiro, reidratado por três dias em tubo PVC e, em seguida, moído a 5mm; 4) Seco, inteiro, reidratado por três dias em tubo PVC com aplicação de ar comprimido e, em seguida, moído a 5mm; 5) Seco, inteiro, reidratado por três dias em tubo PVC e, em seguida, moído a 5mm, e ensilado por 30 dias; 6) Seco, inteiro, reidratado por três dias em tubo PVC com aplicação de ar comprimido, moído a 5mm e ensilado por 30 dias. Os teores de proteína bruta, amido, e fibra em detergente neutro (% da MS) variaram de 9,8 a 10,8%; 59,5 a 67,5%; e de 16,1 a 19,3%, respectivamente. Os teores de umidade nos grãos que foram reidratados variaram de 35,8 a 43,9% da MS. A taxa e a extensão de degradação do amido e a degradabilidade potencial da MS foram semelhantes (P>0,05) entre tratamentos. Os grãos de sorgo reidratados com aplicação de ar comprimido, moídos e ensilados (tratamento 6) apresentaram degradabilidade efetiva (DE) da MS e DE do amido sob taxa de passagem ruminal de 2%/h, semelhantes (P>0,05) às observadas para o tratamento 5 (sem aplicação de ar comprimido), e superiores (P<0,05) às dos demais processamentos. Sob taxas de passagem no rúmen de 5 e 8%/h, as DE do amido estimadas para os grãos processados no tratamento 6 foram superiores (P<0,05) às obtidas para os demais tratamentos. O processamento de reidratação por três dias com aplicação de ar comprimido e ensilagem por trinta dias aumentou a degradabilidade efetiva da matéria seca e do amido dos grãos de sorgo, e sua utilização pode ser recomendada para animais em níveis intermediário e elevado de desempenho...

Used three non-lactating cows fistulated in the rumen in order to determine the parameters of in situ ruminal degradation of dry matter (DM) and starch grain sorghum under different forms of processing: 1) Dried and ground (2mm); 2) Dry, crushed (3mm), rehydrated with water spray on the material and immediately ensiled for 30 days, 3) Dry, round, rehydrated for three days in PVC pipe and then crushed to 5mm, 4 ) Dry, full-rehydrated for three days in PVC tube with application of compressed air and then ground to 5mm; 5) Dry, full-rehydrated for three days in PVC pipe and then ground to 5mm, and ensiled for 30 days; 6) Dry, full-rehydrated for three days in PVC tube with application of compressed air, ground to 5mm and ensiled for 30 days. The crude protein, starch, and neutral detergent fiber (% of DM) ranged from 9.8 to 10.8%, 59.5 to 67.5%, and 16.1 to 19.3%, respectively. The moisture in the grains that were rehydrated ranged from 35.8 to 43.9% DM. The rate and extent of starch degradation and the degradability of MS were similar (P>0.05) between treatments. The grain sorghum rehydrated with application of compressed air, ground and ensiled (treatment 6), showed effective degradability (ED) of DM, and DE starch under ruminal passage rate of 2%/h, similar (P>0.05 ) those observed for treating 5 (without application of compressed air) and higher (P<0.05) to the other processing. Under rumen passage rates of 5 and 8%/h, the estimated NDs starch for processed grains in treatment 6 were higher (P<0.05) than those obtained for the other treatments. Processing rehydration for three days with compressed air application and silage for thirty days increased the effective degradability of dry matter and starch grain sorghum, and its use can be recommended for animals in intermediate and high performance...

Use of F57 bags by the semi-automatic in vitro technique of gas production / Uso de sacos F57 pela técnica in vitro semi-automática de produção de gases

Os cadinhos filtrantes (porosidade 1) originalmente usados na técnica in vitro semi-automática de produção de gases estabelecida no Laboratório de Produção de Gases da Escola de Veterinária da UFMG podem ocasionar interferências nos estudos de quantificação da degradação de alimentos, principalmente os ricos em amido, devido à obstrução dos poros da placa filtrante pelo material residual após fermentação. Este estudo objetivou avaliar a substituição dos cadinhos filtrantes por sacos F57 (Ankom(r)) visando a realização de experimentos in vitro para descrição da cinética de fermentação e degradação ruminal. Esta substituição causou a redução do peso/volume da amostra incubada e desta forma, interferiu na relação pressão (P) / volume (V) e consequentemente inviabilizou a utilização de equação previamente estabelecida por Mauricio et al. (2003). Desta forma, objetivou-se neste estudo estabelecer equação para estimar o volume de gases produzidos a partir de dados de P e V obtidos pela fermentação de sorgo forrageiro, incluindo partes da planta e planta inteira, incubados em sacos F57 (Ankom(r)). Foram quantificados 474 dados simultâneos de pressão e volume os quais variaram de 0 a 4.670 psi e os de volume entre 0 e 38mL. A equação relacionando P e V obtida foi: V (mL) = 0.747 (s.e. 0.0733) + 7.23 P (s.e. 0.1165) + 0.101 P2 (s.e. 0.0372), (R2 = 0.99). O desenvolvimento desta equação viabilizou a utilização dos sacos F57 (Ankom(r)) pela na técnica in vitro semi-automática de produção de gases e, portanto permitindo a quantificação dos resíduos da fermentação favorecendo a acurácia dos resultados...

Valor nutricional de híbridos de sorgo em diferentes estádios de maturação / Nutritional value of hybrids of sorghum in different maturation stages

Avaliaram-se a composição bromatológica e a digestibilidade in vitro das plantas, folhas, colmos e panículas de três híbridos de sorgo (BRS 610, BR 700 e BRS 655) colhidos em três estádios de maturação (leitoso, pastoso e farináceo). O delineamento utilizado foi o inteiramente casualizado, em arranjo fatorial 3x3 (híbridos x estádios de maturação), sendo as médias comparadas pelo teste SNK (P<0,05). Os teores de matéria seca (MS) das plantas variaram de 25,73% a 43,96% e aumentaram com a maturidade. A concentração de proteína bruta (PB) das plantas manteve-se inalterada (P>0,05) entre as idades de corte para todos os híbridos. Os teores de fibra insolúvel em detergente neutro (FDN) e fibra insolúvel em detergente ácido (FDA) das plantas não foram influenciados pelo estádio de maturação. Os coeficientes de digestibilidade in vitro da MS (DIVMS) das plantas do BRS 610 não variaram com a maturidade, mas para o BR 700 e BRS 655 apresentaram redução entre o estádio leitoso e o farináceo. As variações observadas nas porcentagens de MS, PB, FDN, FDA e DIVMS das frações folha, colmo e panícula ocorreram de forma diferente entre os híbridos com o avanço da maturidade. Os híbridos BR 700 e BRS 655 devem ser ensilados no estádio leitoso, enquanto o BRS 610 pode ser colhido nos três estádios de maturação avaliados.

The nutritional value of the plants, leaves, stems and panicles of three hybrids of sorghum (BRS 610, BR 700 and BRS 655) at three maturation stages (milk, soft dough and flour) were evaluated. A complete randomized design was used in a factorial arrangement 3x3 (hybrids x ages of cut), and the means were compared by SNK (P<0.05). Dry matter contents of the plants varied from 25.73% to 43.96% and increased with maturity. The percentage of crude protein (CP) of the plants remained constant (P>0.05) among cuts for all the hybrids. The values of neutral detergent fiber (NDF) and acid detergent fiber (ADF) of the plants were not affected by maturation stages. The in vitro dry matter digestibility (IVDMD) of the plants of BRS 610 didn't change with maturity, but decreased between milk and floury stages for BR 700 and BRS 655. Differences in the percentages of DM, CP, NDF, ADF and IVDMD of the plant fractions (leaves, steam and panicle) occurred differently among hybrids with the advance of maturity. The hybrids BR 700 and BRS 655 should be ensiled at milk stage, while the BRS 610 can be harvested at the three maturation stages evaluated.

[Peripheral precocious puberty: 46, XY complete gonadal dysgenesis]. / Pubertad precoz periférica: disgenesia gonadal completa 46 XY.

Despite standard clinical definitions and availability of diagnostic tests for precocious puberty, an intensive and structured investigation is needed in order to diagnose the aetiology in particular cases. A 4-year-old, phenotypically female child was referred to paediatric endocrinology consultation for premature pubarche and thelarche. There was an acceleration of growth velocity with high levels of estradiol and testosterone, and prepubertal FSH and LH measurements. Investigation showed bilateral gonadoblastoma as the cause of the peripheral precocious puberty. Genetic studies revealed 46 XY karyotype with mutation c.89G> T (p.Arg30Ile) in exon 1 of the SRY gene, confirming the diagnosis of complete gonadal dysgenesis. Disorders of sexual differentiation must be considered in the approach and investigation of peripheral precocious puberty, especially in the presence of ovarian tumours, such as gonadoblastoma and dysgerminoma.

Qualidade da silagem de híbridos de sorgo em diferentes estádios de maturação / Silage quality of sorghum hybrids in different maturation stages

Avaliou-se a qualidade das silagens de três híbridos de sorgo, BRS 610, BR 700 e BRS 655, colhidos em três estádios de maturação da planta, grãos leitoso, pastoso e farináceo. O delineamento utilizado foi o inteiramente ao acaso, em esquema fatorial 3x3 (híbridos x estádios de maturação), com quatro repetições. Os teores de matéria seca apresentaram variação de 22,9% a 41,3%. O BR 700 apresentou maior porcentagem de matéria seca que os demais híbridos em todas as idades de corte. Os teores de proteína bruta e as frações fibrosas, fibra em detergente neutro e fibra em detergente ácido, apresentaram comportamento diferente entre estádios de maturação e entre híbridos. Os valores de lignina não foram alterados com o avanço da idade de corte. Os coeficientes de digestibilidade in vitro da matéria seca das silagens variaram de 50,7% a 55,6% e comportaram-se de forma diferente com a maturidade para cada hibrido. De acordo com os valores de pH, de nitrogênio amoniacal e de ácidos orgânicos encontrados, as silagens dos três híbridos são classificadas como de boa qualidade em todos os períodos de colheita.

The quality of silages of three sorghum hybrids BRS 610, BR 700 and BRS 655 harvested at three maturation stages, milk, soft dough and floury grains, was evaluated. A complete randomized design was used in a 3x3 factorial arrangement (hybrids x maturation stages), with four repetitions. Dry matter values varied from 22.9% to 41.3%. BR 700 had a higher percentage of dry matter than the other hybrids in all ages. The content of crude protein and fibrous fractions, neutral detergent fiber and acid detergent fiber presented different behavior between the maturation stages and between hybrids. The lignin values were not modified with the advancement of the cut age. The in vitro digestibility of silages varied from 50.7% to 55.6% and presented different behavior among each hybrid maturity. According to the pH, ammonia nitrogen, organic acids and dry matter values, the silages of the three hybrids were classified as good fermentation quality at all harvest periods.

Toxic effects of cadmium (Cd²âº) on anaerobic biomass: kinetic and metabolic implications.

Cadmium ion (Cd(2+)) toxicity on anaerobic systems, used for organic matter removal, was assessed by studying its effect on kinetic parameters and metabolic changes. This fundamental study was performed in a continuous fixed bed anaerobic bioreactor that treated synthetic wastewater simulating domestic sewage. The biomass was immobilized on a fixed bed made of polyurethane foam. Under influent cadmium concentrations of 0.0, 0.4, 4.4 and 6.2 mg Cd(2+) L(-1) the organic matter removal efficiencies were 84%, 82%, 72% and 52%, respectively. At influent concentration of 6.2 mg Cd(2+) L(-1) the reactor had reached its limit for cadmium toxicity. In the removal of dissolved organic matter, the first-order apparent kinetic coefficients (k(1)(app)) were 0.84, 0.67 and 0.10 h(-1) for the operations with 0.0, 0.4 and 4.4 mg Cd(2+) L(-1), respectively. The apparent inhibition coefficient for cadmium (k(i)(app)) was 1.69 mg L(-1). Despite the toxic effects of cadmium on anaerobic organic matter removal at large Cd(2+) concentrations, the results demonstrated that the anaerobic process was suitable for cadmium concentrations below 29.8 mg Cd(2+) L(-1), considering the bioavailable fraction of adsorbed cadmium in the support when the cadmium influent concentration was 6.2 mg Cd(2+) L(-1).

Consumo e digestibilidade aparente de silagens de sorgo em diferentes estádios de maturação / Intake and apparent digestibility of sorghum silages at different maturation stages

Avaliaram-se o consumo e a digestibilidade aparente, em ovinos, das silagens dos híbridos de sorgo BRS 610, BR 700 e BRS 655 colhidos em três estádios de maturação dos grãos: leitoso, pastoso e farináceo. Os valores de consumo da matéria seca, em gramas por quilograma de unidade de tamanho metabólico (CMS-UTM), variaram de 45,90g/UTM/dia a 59,99g/UTM/dia e não diferiram com o avanço do estádio de maturação para todos os híbridos (P>0,05). No estádio pastoso, o BRS 655 apresentou menor CMS-UTM em relação aos demais híbridos (P<0,05), que foram semelhantes entre si (P>0,05). Foi observado menor consumo de proteína digestível para os híbridos com tanino, BR 700 e BRS 655, em relação ao BRS 610 (P<0,05) nos estádios pastoso e farináceo. Todos os tratamentos apresentaram balanço de nitrogênio levemente positivo, com valores entre 0,02g/UTM/dia e 0,20g/UTM/dia. Não houve diferenças nos valores de digestibilidade da fibra entre os híbridos com tanino, BR 700 e BRS 655, e sem tanino, BRS 610. Recomenda-se a utilização da silagem do híbrido BRS 610, colhida no estádio pastoso. Os híbridos BRS 655 e BR 700 devem ser colhidos no estádio leitoso.

The intake and apparent digestibility of the silages of the sorghum hybrids BRS 610, BR 700 and BRS 655 harvested at three maturation stages of milk, soft dough and floury grains by sheep were evaluated. The dry matter intake, in grams per metabolic weight (DMI-MW), ranged from 45.90 to 59.99g/MW/day and didn't change with maturity (P>0.05) for all the hybrids. At the soft dough stage, BRS 655 presented lower DMI-MW (P<0.05) than the other hybrids, which were similar (P>0.05). The hybrids with tannin (BR 700 and BRS 655) had lower intakes of digestible protein than the BRS 610 at the soft dough and floury stages (P<0.05). All the treatments showed slight positive nitrogen balance which varied from 0.02g/MW/d to 0.20g/MW/d. No differences were observed in fiber digestibility among hybrids with tannins (BR 700 e BRS 655) and without tannins (BRS 610). The use of BRS 610 is recommended and should be ensilaged at soft dough stage. The hybrids BR 655 and BR 700 should be harvested at milk stage.

Effect of grain maturity stage on the quality of sorghum BRS-610 silages / Efeito do estádio de maturação dos grãos na qualidade das silagens do sorgo BRS-610

The quality of sorghum BRS-610 silages in seven stages of grain maturity, between the milky and dry stage, for determination of the ideal moment of ensilage was evaluated. The silos were open after 56 days of fermentation. The values for dry matter of silages increased from 199 to 473g/kg or from 19.9 to 47.3 percent, with the advance of stage maturity of grains. As for the quality of fermentation, the silages may be classified as excellent quality in every stage of maturity. The lower values of neutral detergent fiber, 539g/kg or 53.9 percent, and acid detergent fiber, 307g/kg or 30.7 percent, were observed between the milky/dough to dough/dent stages. Regression analysis indicated an increase in values of lignin - 0.3 unit por stage - and reduction in values of in vitro dry matter digestibility - -2.5 units por stage - with the advance of the grain's stage maturity. The ensilage of sorghum BRS-610 between the milky/dough and dough stage ensure very good fermentation and nutritive value of silage.

Avaliou-se a qualidade das silagens do sorgo BRS-610 em sete estádios de maturação dos grãos, entre o estádio leitoso a estádio seco, para determinar o momento ideal de ensilagem. Os silos foram abertos após 56 dias de fermentação. Os teores de matéria seca das silagens aumentaram de 199 para 473g/kg, ou de 19,9 para 47,3 por cento, com o avanço do estádio de maturação dos grãos. Quanto à qualidade de fermentação, as silagens foram classificadas como de excelente qualidade para todos os estádios de maturação. Os baixos valores de fibra detergente neutro, 539g/kg ou 53,9 por cento, e fibra detergente ácido, 307g/kg ou 30,7 por cento, foram observados entre os estádios leitoso/pastoso a pastoso/farináceo. A análise de regressão indicou aumento nos teores de lignina, 0,35 unidades por estádio, e redução nos valores de digestibilidade in vitro da matéria seca, -2,50 unidades por estádio, com o avanço do estádio de maturação dos grãos. A ensilagem do sorgo BRS-610 entre os estádios de grãos leitoso/pastoso a pastoso, garante excelente fermentação e bom valor nutricional da silagem.

9 Years after the first laparoscopic adjusted gastric banding (LAGB) in adolescents: the Portuguese experience.

INTRODUCTION: Children all over the world are increasingly becoming overweight and obese. The experience with adults has demonstrated that surgery is the only effective way of achieving sustained weight loss in obese patients. Simultaneously, it has been proposed that bariatric surgery in the adolescent period would be a more effective treatment for childhood-onset extreme obesity rather than delaying surgery for extremely obese youth until adulthood. Nevertheless, the optimal surgical option for both adults and adolescents has yet to be established. The aim of our study was to evaluate the results of 9 years of laparoscopic adjusted gastric banding (LAGB) performed in our hospital for obesity-associated comorbidities (OAC), weight loss and surgical morbidity. METHODS: A prospective study of all patients who underwent LAGB at our hospital between July 2001 and May 2010 was carried out. Patient selection was by a multidisciplinary team and based on the "IPEG Guideline for the Surgical Treatment of Extremely Obese Adolescents". Data were reviewed retrospectively. RESULTS: 14 patients underwent LAGB (10♀; 4♂; average age: 16 years). Preoperative BMI ranged between 41.4 and 54.6 kg/m (2). Prior to surgery, 8 different OACs were identified. 6 months later, 4 types of OAC had resolved completely and the other 4 had improved, and the improvement was maintained over time. The average excess weight (EW) loss increased from 25.7 to 48.2% at 6 and 36 months of follow-up, respectively (10 patients). At 60 and 106 months of follow-up it was 41.5% (5 patients) and 31.8% (1 patient), respectively. There were no complications arising from the surgery itself. Long-term complications were few and easily managed. CONCLUSIONS: LAGB seems to be a good option to treat obese adolescents, as it is a minimally invasive procedure that does not radically change the patient's anatomy and is associated with minimal morbidity. It leads to a sustained improvement/resolution of OAC, and although weight loss is not continuous, it is maintained over time.

Avaliação das silagens do sorgo BRS-610 em sete estádios de maturação pela técnica in vitro semiautomática de produção de gases / Evaluation of the silages of sorghum BRS-610 at seven stages of maturation by semi-automated in vitro gas production technique

Avaliou-se a qualidade da silagem do sorgo BRS-610 produzida em sete estádios de maturação dos grãos, pela técnica in vitro semiautomática de produção de gases. O delineamento foi inteiramente ao acaso, com quatro repetições por tratamento, e as médias foram comparadas pelo teste Scott-knott. Os parâmetros potencial máximo de produção de gases, tempo de colonização, taxa de produção de gases e degradabilidade efetiva da matéria seca para as taxas de passagem de 2, 5 e 8 por cento/h foram obtidos pelo modelo de France et al. O potencial máximo de produção de gases não diferiu entre as silagens e indicou média de 170,5mL/g de matéria seca. Entre leitoso/pastoso e pastoso houve menor tempo de colonização. As maiores taxas de fermentação foram observadas nas silagens produzida entre os estádios pastoso/farináceo a seco. As degradabilidades efetivas foram semelhantes entres as silagens, com média de 46,2 por cento. O sorgo BRS-610 produziu silagem de qualidade satisfatória, com destaque para a silagem produzida com a planta no estádio pastoso/farináceo, por associar melhor taxa de fermentação e menor tempo de colonização.

The quality of the silages of sorghum BRS-610 produced at seven stages of grain maturation was evaluated by the semi-automatic gas production technique. It was used a complete randomized design, with four repetitions per treatment, and the means were compared by the Scott-knott test (P<0.05). The potential of production of gases, lag phase, rates of gas production, and effective dry matter degradability for 2, 5, and 8 percent/h rates of passage were obtained by the model of France et al. The potential of gas production did not differ among the silages, showing mean of 170.5mL/dry matter. The stages between milky/soft dough to soft dough showed shorter lag phase. The highest rate of gas production was observed in the silages produced between soft dough/floury to dry stages. The effective degradabilities were similar among the silages, averaging 46.2 percent. The sorghum BRS-610 produced satisfactory quality silage, mainly for the silage produced with the plant at soft dough/floury stage, for associating better rate of gas production and shorter lag phase.

Produção e composição bromatológica de vinte e cinco genótipos de sorgo (sorghum bicolor (L. ) Moench) / Chemical composition and production of 25 hybrids of sorghum (sorghum bicolor (L. ) Moench)

Chemical composition and dry matter (DM) productivity of 25 genotypes of sorghum were obtained. The experiment was carried out in a randomized block design with 25 treatments and three replications each. The data were submitted to analysis of variance and the means grouped by Scott-Knot Test (5 percent). The level of DM productivity varied from 6.82 to 14.17ton/hectare, and the higher level was obtained by VOLUMAX genotype (16.09 ton/hectare). The levels of crude protein varied from 5.24 to 7.79 percent. No differences were detected between the genotypes to neutral detergent fiber levels, which varied from 59.03 to 73.04 percent. Considering the level of acid detergent fiber, the variation interval was 26.78 to 40.92 percent. The better genotypes for silage production were ATF54*9929036, CMSXS212*9929048, CMSXS217*9929012, and VOLUMAX.