RESUMO
Sustained enhancement of axonal signaling and increased neurotransmitter release by the activation of pre-synaptic nicotinic acetylcholine receptors (nAChRs) is an important mechanism for neuromodulation by acetylcholine (ACh). The difficulty with access to probing the signaling mechanisms within intact axons and at nerve terminals both in vitro and in vivo has limited progress in the study of the pre-synaptic components of synaptic plasticity. Here we introduce a gene-chimeric preparation of ventral hippocampal (vHipp)-accumbens (nAcc) circuit in vitro that allows direct live imaging to analyze both the pre- and post-synaptic components of transmission while selectively varying the genetic profile of the pre- vs post-synaptic neurons. We demonstrate that projections from vHipp microslices, as pre-synaptic axonal input, form multiple, reliable glutamatergic synapses with post-synaptic targets, the dispersed neurons from nAcc. The pre-synaptic localization of various subtypes of nAChRs are detected and the pre-synaptic nicotinic signaling mediated synaptic transmission are monitored by concurrent electrophysiological recording and live cell imaging. This preparation also provides an informative approach to study the pre- and post-synaptic mechanisms of glutamatergic synaptic plasticity in vitro.
Assuntos
Axônios/metabolismo , Região CA1 Hipocampal/metabolismo , Sinalização do Cálcio/fisiologia , Nicotina/metabolismo , Animais , Região CA1 Hipocampal/citologia , Técnicas de Cocultura , Camundongos , Camundongos Transgênicos , Plasticidade Neuronal , Neurônios/metabolismo , Neurotransmissores/metabolismo , Núcleo Accumbens/citologia , Núcleo Accumbens/metabolismo , Receptores Nicotínicos/metabolismo , Sinapses/metabolismo , Transmissão SinápticaRESUMO
Presynaptic nicotinic acetylcholine receptors (nAChRs) have long been implicated in the modulation of CNS circuits. We previously reported that brief exposure to low concentrations of nicotine induced sustained potentiation of glutamatergic transmission at ventral hippocampal (vHipp)-striatal synapses. Here, we exploited nAChR subtype-selective antagonists and agonists and α7*nAChR knockout mutant mice (α7-/-) to elucidate the signaling mechanisms underlying nAChR-mediated modulation of synaptic transmission. Using a combination of micro-slices culture from WT and α7-/-mice, calcium imaging, and immuno-histochemical techniques, we found that nicotine elicits localized and oscillatory increases in intracellular Ca(2+) along vHipp axons that persists for up to 30 minutes. The sustained phase of the nicotine-induced Ca(2+) response was blocked by α-BgTx but not by DHßE and was mimicked by α7*nAChR agonists but not by non-α7*nAChR agonists. In vHipp slices from α7-/- mice, nicotine elicited only transient increases of axonal Ca(2+) signals and did not activate CaMKII. The sustained phase of the nicotine-induced Ca(2+) response required localized activation of CaMKII, phospholipase C, and IP3 receptor mediated Ca(2+)-induced Ca(2+) release (CICR). In conclusion, activation of presynaptic nAChRs by nicotine elicits Ca(2+) influx into the presynaptic axons, the sustained phase of the nicotine-induced Ca(2+) response requires that axonal α7*nAChR activate a downstream signaling network in the vHipp axons.
Assuntos
Axônios/metabolismo , Sinalização do Cálcio , Hipocampo/efeitos dos fármacos , Hipocampo/metabolismo , Nicotina/farmacologia , Animais , Cálcio/metabolismo , Proteína Quinase Tipo 2 Dependente de Cálcio-Calmodulina/metabolismo , Técnicas de Cocultura , Receptores de Inositol 1,4,5-Trifosfato/metabolismo , Espaço Intracelular , Camundongos , Camundongos Knockout , Plasticidade Neuronal , Sinapses/metabolismo , Receptor Nicotínico de Acetilcolina alfa7/genética , Receptor Nicotínico de Acetilcolina alfa7/metabolismoRESUMO
The dorsomedial nucleus of the hypothalamus (DMH) contributes to the regulation of overall energy homeostasis by modulating energy intake as well as energy expenditure. Despite the importance of the DMH in the control of energy balance, DMH-specific genetic markers or neuronal subtypes are poorly defined. Here we demonstrate the presence of cholinergic neurons in the DMH using genetically modified mice that express enhanced green florescent protein (eGFP) selectively in choline acetyltransferase (Chat)-neurons. Overnight food deprivation increases the activity of DMH cholinergic neurons, as shown by induction of fos protein and a significant shift in the baseline resting membrane potential. DMH cholinergic neurons receive both glutamatergic and GABAergic synaptic input, but the activation of these neurons by an overnight fast is due entirely to decreased inhibitory tone. The decreased inhibition is associated with decreased frequency and amplitude of GABAergic synaptic currents in the cholinergic DMH neurons, while glutamatergic synaptic transmission is not altered. As neither the frequency nor amplitude of miniature GABAergic or glutamatergic postsynaptic currents is affected by overnight food deprivation, the fasting-induced decrease in inhibitory tone to cholinergic neurons is dependent on superthreshold activity of GABAergic inputs. This study reveals that cholinergic neurons in the DMH readily sense the availability of nutrients and respond to overnight fasting via decreased GABAergic inhibitory tone. As such, altered synaptic as well as neuronal activity of DMH cholinergic neurons may play a critical role in the regulation of overall energy homeostasis.
Assuntos
Neurônios Colinérgicos/fisiologia , Núcleo Hipotalâmico Dorsomedial/fisiologia , Metabolismo Energético/fisiologia , Potenciais da Membrana/fisiologia , Animais , Colina O-Acetiltransferase/metabolismo , Neurônios Colinérgicos/citologia , Núcleo Hipotalâmico Dorsomedial/citologia , Jejum , Genes Reporter , Proteínas de Fluorescência Verde , Camundongos , Camundongos Transgênicos , Proteínas Proto-Oncogênicas c-fos/genética , Receptores de GABA/fisiologia , Receptores de Glutamato/fisiologia , Transmissão SinápticaRESUMO
Neuregulin 1 (Nrg1), a schizophrenia susceptibility gene, is involved in fundamental aspects of neurodevelopment. Mice lacking any one of the several isoforms of Nrg1 have a variety of schizophrenia-related phenotypes, including deficits in working memory and sensorimotor gating, loss of spines in pyramidal neurons in the ventral subiculum, loss of dendrites in cortical pyramidal cells, loss of parvalbumin-positive interneurons in the prefrontal cortex, and altered plasticity in corticolimbic synapses. Mice heterozygous for a disruption in exon 7 of the Nrg1 gene lack Type III (cysteine-rich-domain-containing) isoforms and have sensorimotor gating deficits that may involve changes in the activity of a circuit involving projections from the ventral hippocampus (vHPC) to medium spiny neurons in the nucleus accumbens (nACC). To explore the neural basis of these deficits, we examined electrophysiological activity in the nACC and vHPC of these mice. Under urethane anesthesia, bursts of spontaneous activity propagated from the vHPC to the nACC in both wild-type and mutant mice. However, these bursts were weaker in mutant nACC, with reduced local field potential amplitude and spiking activity. Single units in mutant nACC fired less frequently within the bursts, and more frequently outside of the bursts. Moreover, within-burst nACC spiking was less modulated by vHPC activity, as determined by phase-locking to the low-frequency oscillatory components of the bursts. These data suggest that the efficacy of vHPC input to the nACC is reduced in the Type III Nrg1 heterozygotes, supporting a role for Nrg1 in the functional profile of hippocampal-accumbens synapses.
Assuntos
Hipocampo/metabolismo , Vias Neurais/metabolismo , Neuregulina-1/deficiência , Neuregulina-1/genética , Núcleo Accumbens/metabolismo , Potenciais de Ação/genética , Animais , Cisteína/genética , Predisposição Genética para Doença/genética , Hipocampo/patologia , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Knockout , Camundongos Transgênicos , Vias Neurais/patologia , Neurônios/metabolismo , Neurônios/patologia , Núcleo Accumbens/patologia , Isoformas de Proteínas/genética , Estrutura Terciária de Proteína/genéticaRESUMO
Neuregulin 1 (NRG1) signaling is critical to various aspects of neuronal development and function. Among different NRG1 isoforms, the type III isoforms of NRG1 are unique in their ability to signal via the intracellular domain after gamma-secretase-dependent intramembranous processing. However, the functional consequences of type III NRG1 signaling via its intracellular domain are mostly unknown. In this study, we have identified mutations within type III NRG1 that disrupt intramembranous proteolytic processing and abolish intracellular domain signaling. In particular, substitutions at valine 321, previously linked to schizophrenia risks, result in NRG1 proteins that fail to undergo gamma-secretase-mediated nuclear localization and transcriptional activation. Using processing-defective mutants of type III NRG1, we demonstrate that the intracellular domain signaling is specifically required for NRG1 regulation of the growth and branching of cortical dendrites but not axons. Consistent with the role of type III NRG1 signaling via the intracellular domain in the initial patterning of cortical dendrites, our findings from pharmacological and genetic studies indicate that type III NRG1 functions in dendritic development independent of ERBB kinase activity. Together, these results support the proposal that aberrant intramembranous processing and defective signaling via the intracellular domain of type III NRG1 impair a subset of NRG1 functions in cortical development and contribute to abnormal neuroconnectivity implicated in schizophrenia.
Assuntos
Córtex Cerebral/citologia , Neuregulina-1/metabolismo , Neurônios/metabolismo , Valina/metabolismo , Alanina/genética , Animais , Axônios/metabolismo , Células Cultivadas , Córtex Cerebral/embriologia , Proteínas de Ligação a DNA/metabolismo , Embrião de Mamíferos , Receptores ErbB/deficiência , Feminino , Proteínas de Fluorescência Verde/genética , Humanos , Indóis , Leucina/genética , Proteínas Luminescentes/genética , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Transgênicos , Proteínas Associadas aos Microtúbulos/metabolismo , Mutação/genética , Neuregulina-1/deficiência , Neurônios/citologia , Receptor ErbB-4 , Estatísticas não Paramétricas , Transfecção/métodos , Valina/genética , Proteína Vesicular 1 de Transporte de Glutamato/metabolismoRESUMO
Nicotine is the principle addictive agent delivered via cigarette smoking. The addictive activity of nicotine is due to potent interactions with nicotinic acetylcholine receptors (nAChRs) on neurons in the reinforcement and reward circuits of the brain. Beyond its addictive actions, nicotine is thought to have positive effects on performance in working memory and short-term attention-related tasks. The brain areas involved in such behaviors are part of an extensive cortico-limbic network that includes relays between prefrontal cortex (PFC) and cingulate cortex (CC), hippocampus, amygdala, ventral tegmental area (VTA) and the nucleus accumbens (nAcc). Nicotine activates a broad array of nAChRs subtypes that can be targeted to pre- as well as peri- and post-synaptic locations in these areas. Thereby, nicotine not only excites different types of neurons, but it also perturbs baseline neuronal communication, alters synaptic properties and modulates synaptic plasticity. In this review we focus on recent findings on nicotinic modulation of cortical circuits and their targets fields, which show that acute and transient activation of nicotinic receptors in cortico-limbic circuits triggers a series of events that affects cognitive performance in a long lasting manner. Understanding how nicotine induces long-term changes in synapses and alters plasticity in the cortico-limbic circuits is essential to determining how these areas interact in decoding fundamental aspects of cognition and reward.
Assuntos
Plasticidade Neuronal/efeitos dos fármacos , Nicotina/farmacologia , Transmissão Sináptica/efeitos dos fármacos , Animais , Humanos , Tabagismo/fisiopatologiaRESUMO
Both the neuregulin 1 (Nrg1) and alpha7 nicotinic acetylcholine receptor (alpha7*nAChRs) genes have been linked to schizophrenia and associated sensory-motor gating deficits. The prominence of nicotine addiction in schizophrenic patients is reflected in the normalization of gating deficits by nicotine self-administration. To assess the role of presynaptic type III Nrg1 at hippocampal-accumbens synapses, an important relay in sensory-motor gating, we developed a specialized preparation of chimeric circuits in vitro. Synaptic relays from Nrg1(tm1Lwr) heterozygote ventral hippocampal slices to wild-type (WT) nucleus accumbens neurons (1) lack a sustained, alpha7*nAChRs-mediated phase of synaptic potentiation seen in comparable WT/WT circuits and (2) are deficient in targeting alpha7*nAChRs to presynaptic sites. Thus, selective alteration of the level of presynaptic type III Nrg1 dramatically affects the modulation of glutamatergic transmission at ventral hippocampal to nucleus accumbens synapses.
Assuntos
Hipocampo/fisiologia , Peptídeos e Proteínas de Sinalização Intracelular/fisiologia , Nicotina/farmacologia , Agonistas Nicotínicos/farmacologia , Terminações Pré-Sinápticas/metabolismo , Receptores Nicotínicos/fisiologia , Transmissão Sináptica/efeitos dos fármacos , Animais , Bungarotoxinas/farmacologia , Cicloeximida/farmacologia , Antagonistas de Aminoácidos Excitatórios/farmacologia , Potenciais Pós-Sinápticos Excitadores/efeitos dos fármacos , Potenciais Pós-Sinápticos Excitadores/fisiologia , Potenciais Pós-Sinápticos Excitadores/efeitos da radiação , Regulação da Expressão Gênica/efeitos dos fármacos , Regulação da Expressão Gênica/genética , Glutamato Descarboxilase/metabolismo , Ácido Glutâmico/metabolismo , Peptídeos e Proteínas de Sinalização Intracelular/deficiência , Camundongos , Camundongos Knockout , Neurregulinas , Núcleo Accumbens/fisiologia , Técnicas de Cultura de Órgãos , Técnicas de Patch-Clamp/métodos , Inibidores da Síntese de Proteínas/farmacologia , Transmissão Sináptica/fisiologia , Fatores de Tempo , Proteína Vesicular 1 de Transporte de Glutamato/metabolismo , Receptor Nicotínico de Acetilcolina alfa7 , Ácido gama-Aminobutírico/metabolismoRESUMO
Type III Neuregulin1 (Nrg1) isoforms are membrane-tethered proteins capable of participating in bidirectional juxtacrine signaling. Neuronal nicotinic acetylcholine receptors (nAChRs), which can modulate the release of a rich array of neurotransmitters, are differentially targeted to presynaptic sites. We demonstrate that Type III Nrg1 back signaling regulates the surface expression of alpha7 nAChRs along axons of sensory neurons. Stimulation of Type III Nrg1 back signaling induces an increase in axonal surface alpha7 nAChRs, which results from a redistribution of preexisting intracellular pools of alpha7 rather than from increased protein synthesis. We also demonstrate that Type III Nrg1 back signaling activates a phosphatidylinositol 3-kinase signaling pathway and that activation of this pathway is required for the insertion of preexisting alpha7 nAChRs into the axonal plasma membrane. These findings, in conjunction with prior results establishing that Type III Nrg1 back signaling controls gene transcription, demonstrate that Type III Nrg1 back signaling can regulate both short-and long-term changes in neuronal function.
Assuntos
Axônios/metabolismo , Neuregulina-1/metabolismo , Proteínas Oncogênicas v-erbB/metabolismo , Isoformas de Proteínas/metabolismo , Receptores Nicotínicos/metabolismo , Receptores Pré-Sinápticos/metabolismo , Transdução de Sinais/fisiologia , Animais , Axônios/ultraestrutura , Células Cultivadas , Embrião de Mamíferos/anatomia & histologia , Endocitose/fisiologia , Gânglios Espinais/citologia , Regulação da Expressão Gênica , Camundongos , Camundongos Knockout , Neuregulina-1/genética , Proteínas Oncogênicas v-erbB/genética , Fosfatidilinositol 3-Quinases/genética , Fosfatidilinositol 3-Quinases/metabolismo , Isoformas de Proteínas/genética , Proteínas Proto-Oncogênicas c-akt/genética , Proteínas Proto-Oncogênicas c-akt/metabolismo , Receptores Nicotínicos/genética , Receptores Pré-Sinápticos/genética , Receptor Nicotínico de Acetilcolina alfa7RESUMO
The basolateral nucleus of the amygdala (BLA) receives cholinergic innervation from the basal forebrain and nicotine, via activation of neuronal nicotinic acetylcholine receptors (nAChRs), can improve performance in amygdala-based learning tasks. We tested the hypothesis that acute and prenatal nicotine exposure modulates cortico-amygdala synaptic transmission. We found that low-dose, single-trial exposures to nicotine can elicit lasting facilitation, the extent of which is dependent on the level of stimulation of the cortical inputs to the BLA. In addition, sustained facilitation is ablated by prenatal exposure to nicotine. This study examined synaptic transmission in 238 patch-clamp recordings from BLA neurons in acute slice from mouse brain. Pharmacological studies in wild-type and nAChR subunit knock-out mice reveal that activation of presynaptic alpha 7, containing (alpha 7*) and non-alpha 7* nAChRs, facilitates glutamatergic transmission in an activity-dependent manner. Without prior stimulation, application of nicotine elicits modest and transient facilitation of glutamatergic postsynaptic currents (PSCs) in about 40% of BLA neurons. With low-frequency stimulation of cortical inputs nicotine elicits robust facilitation of transmission at about 60% of cortico-BLA synapses and synaptic strength remains elevated at about 40% of these connections for >15 min after nicotine washout. Following paired-pulse stimulation nicotine elicits long-lasting facilitation of glutamatergic transmission at about 70% of cortico-BLA connections. Nicotine reduces the threshold for activation of long-term potentiation of cortico-BLA synapses evoked by patterned stimulation. Prenatal exposure to nicotine reduced subsequent modulatory responses to acute nicotine application.
Assuntos
Tonsila do Cerebelo/efeitos dos fármacos , Córtex Cerebral/efeitos dos fármacos , Ácido Glutâmico/fisiologia , Nicotina/farmacologia , Agonistas Nicotínicos/farmacologia , Sinapses/efeitos dos fármacos , Transmissão Sináptica/efeitos dos fármacos , Tonsila do Cerebelo/citologia , Animais , Animais Geneticamente Modificados , Córtex Cerebral/citologia , Potenciais Pós-Sinápticos Excitadores/efeitos dos fármacos , Feminino , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Knockout , Gravidez , Efeitos Tardios da Exposição Pré-Natal , Células Piramidais/fisiologia , Receptores Nicotínicos/genética , Receptores Nicotínicos/fisiologia , Receptor Nicotínico de Acetilcolina alfa7RESUMO
Neuronal migration and axon guidance constitute fundamental processes in brain development that are generally studied independently. Although both share common mechanisms of cell biology and biochemistry, little is known about their coordinated integration in the formation of neural circuits. Here we show that the development of the thalamocortical projection, one of the most prominent tracts in the mammalian brain, depends on the early tangential migration of a population of neurons derived from the ventral telencephalon. This tangential migration contributes to the establishment of a permissive corridor that is essential for thalamocortical axon pathfinding. Our results also demonstrate that in this process two different products of the Neuregulin-1 gene, CRD-NRG1 and Ig-NRG1, mediate the guidance of thalamocortical axons. These results show that neuronal tangential migration constitutes a novel mechanism to control the timely arrangement of guidance cues required for axonal tract formation in the mammalian brain.
Assuntos
Axônios/fisiologia , Movimento Celular , Córtex Cerebral/citologia , Neuregulina-1/metabolismo , Tálamo/citologia , Animais , Transporte Biológico , Células COS , Córtex Cerebral/metabolismo , Chlorocebus aethiops , Receptores ErbB/deficiência , Cistos Glanglionares/metabolismo , Camundongos , Camundongos Transgênicos , Neuregulina-1/deficiência , Neuregulina-1/genética , Isoformas de Proteínas , RNA Mensageiro/genética , RNA Mensageiro/metabolismo , Receptor ErbB-4 , Telencéfalo/citologia , Tálamo/metabolismoRESUMO
Recently developed therapeutics for obesity, targeted against cannabinoid receptors, result in decreased appetite and sustained weight loss. Prior studies have demonstrated CB1 receptors (CB1Rs) and leptin modulation of cannabinoid synthesis in hypothalamic neurons. Here, we show that depolarization of perifornical lateral hypothalamus (LH) neurons elicits a CB1R-mediated suppression of inhibition in local circuits thought to be involved in appetite and "natural reward." The depolarization-induced decrease in inhibitory tone to LH neurons is blocked by leptin. Leptin inhibits voltage-gated calcium channels in LH neurons via the activation of janus kinase 2 (JAK2) and of mitogen-activated protein kinase (MAPK). Leptin-deficient mice are characterized by both an increase in steady-state voltage-gated calcium currents in LH neurons and a CB1R-mediated depolarization-induced suppression of inhibition that is 6-fold longer than that in littermate controls. Our data provide direct electrophysiological support for the involvement of endocannabinoids and leptin as modulators of hypothalamic circuits underlying motivational aspects of feeding behavior.
Assuntos
Regulação do Apetite/fisiologia , Moduladores de Receptores de Canabinoides/metabolismo , Endocanabinoides , Região Hipotalâmica Lateral/metabolismo , Leptina/metabolismo , Vias Neurais/metabolismo , Transdução de Sinais/fisiologia , Animais , Regulação do Apetite/efeitos dos fármacos , Canais de Cálcio/efeitos dos fármacos , Canais de Cálcio/metabolismo , Sinalização do Cálcio/efeitos dos fármacos , Sinalização do Cálcio/genética , Moduladores de Receptores de Canabinoides/farmacologia , Região Hipotalâmica Lateral/efeitos dos fármacos , Hipotálamo/efeitos dos fármacos , Hipotálamo/fisiologia , Janus Quinase 2 , Leptina/genética , Leptina/farmacologia , Sistema de Sinalização das MAP Quinases/efeitos dos fármacos , Sistema de Sinalização das MAP Quinases/fisiologia , Potenciais da Membrana/efeitos dos fármacos , Potenciais da Membrana/fisiologia , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Transgênicos , Motivação , Inibição Neural/efeitos dos fármacos , Inibição Neural/fisiologia , Vias Neurais/efeitos dos fármacos , Neurônios/efeitos dos fármacos , Neurônios/metabolismo , Técnicas de Cultura de Órgãos , Técnicas de Patch-Clamp , Proteínas Tirosina Quinases/metabolismo , Proteínas Proto-Oncogênicas/metabolismo , Receptor CB1 de Canabinoide/antagonistas & inibidores , Receptor CB1 de Canabinoide/metabolismo , Receptores para Leptina , Recompensa , Transdução de Sinais/efeitos dos fármacos , Fatores de TempoRESUMO
Nicotine administration reduces appetite and alters feeding patterns; a major deterrent to smoking cessation is hyperphagia and resultant weight gain. We demonstrate here that lateral hypothalamic (LH) circuits involving melanin-concentrating hormone (MCH) neurons are subject to cholinergic modulation that may be related to the effects of nicotine on appetite control. Cholinergic input to the perifornical LH area of the mouse is confirmed by examination of immunostaining for vesicular acetylcholine (ACh) transporter (VAT) in conjunction with antibodies to MCH and the vesicular GABA transporter (vGABAT). vAChT-positive neurons border the LH, and VAT-positive projections are detected throughout the perifornical area. MCH-positive dendrites appear studded with vGABAT-positive contacts, consistent with recordings of GABAergic inputs to LH/MCH neurons identified by their location, morphology, electrophysiological profile, and MCH expression. Activation of presynaptic nicotinic ACh receptors (nAChRs) enhances GABAergic transmission. GABAergic transmission is potentiated by (1) direct nicotine application, (2) increasing local ACh concentration, and (3) stimulation of cholinergic projections. Based on pharmacological studies and comparisons of wild-type versus alpha7 nAChR subunit mutant mice, we propose that alpha7*-nAChRs are required for the modulation of GABAergic inputs in LH. Prenatal exposure to nicotine elicits a persistent elevation of GABAergic transmission in the LH of postnatal pups. Furthermore, GABAergic inputs to LH of prenatal nicotine-exposed pups are insensitive to subsequent nicotine challenge. Our studies support the hypothesis that nicotine administration or elevated cholinergic tone enhance inhibition of perifonical LH/MCH neurons via activation of presynaptic alpha7*-nAChRs.
Assuntos
Apetite/fisiologia , Fibras Colinérgicas/fisiologia , Região Hipotalâmica Lateral/fisiologia , Hormônios Hipotalâmicos/metabolismo , Melaninas/metabolismo , Hormônios Hipofisários/metabolismo , Sinapses/fisiologia , Acetilcolina/metabolismo , Acetilcolina/fisiologia , Animais , Animais Recém-Nascidos , Feminino , Região Hipotalâmica Lateral/citologia , Região Hipotalâmica Lateral/metabolismo , Técnicas In Vitro , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Knockout , Inibição Neural/efeitos dos fármacos , Neurônios/metabolismo , Neurônios/fisiologia , Nicotina/farmacologia , Gravidez , Efeitos Tardios da Exposição Pré-Natal , Receptores Colinérgicos/fisiologia , Receptores Nicotínicos/metabolismo , Transmissão Sináptica/fisiologia , Receptor Nicotínico de Acetilcolina alfa7 , Ácido gama-Aminobutírico/metabolismoRESUMO
In the nervous system of vertebrates, myelination is essential for rapid and accurate impulse conduction. Myelin thickness depends on axon fiber size. We use mutant and transgenic mouse lines to show that axonal Neuregulin-1 (Nrg1) signals information about axon size to Schwann cells. Reduced Nrg1 expression causes hypomyelination and reduced nerve conduction velocity. Neuronal overexpression of Nrg1 induces hypermyelination and demonstrates that Nrg1 type III is the responsible isoform. We suggest a model by which myelin-forming Schwann cells integrate axonal Nrg1 signals as a biochemical measure of axon size.
Assuntos
Axônios/fisiologia , Axônios/ultraestrutura , Bainha de Mielina/fisiologia , Bainha de Mielina/ultraestrutura , Neuregulina-1/fisiologia , Animais , Receptores ErbB/análise , Receptores ErbB/fisiologia , Gânglios Espinais/química , Marcação de Genes , Genes erbB , Genes erbB-2 , Heterozigoto , Camundongos , Camundongos Knockout , Camundongos Transgênicos , Modelos Neurológicos , Condução Nervosa , Neuregulina-1/genética , Isoformas de Proteínas/fisiologia , Receptor ErbB-2/análise , Receptor ErbB-2/fisiologia , Receptor ErbB-3/análise , Receptor ErbB-3/fisiologia , Células de Schwann/fisiologia , Nervo Isquiático/química , Transdução de SinaisRESUMO
Transmembrane isoforms of neuregulin-1 (Nrg-1), ligands for erbB receptors, include an extracellular domain with an EGF-like sequence and a highly conserved intracellular domain (ICD) of unknown function. In this paper, we demonstrate that transmembrane isoforms of Nrg-1 are bidirectional signaling molecules in neurons. The stimuli for Nrg-1 back signaling include binding of erbB receptor dimers to the extracellular domain of Nrg-1 and neuronal depolarization. These stimuli elicit proteolytic release and translocation of the ICD of Nrg-1 to the nucleus. Once in the nucleus, the Nrg-1 ICD represses expression of several regulators of apoptosis, resulting in decreased neuronal cell death in vitro. Thus, regulated proteolytic processing of Nrg-1 results in retrograde signaling that appears to mediate contact and activity-dependent survival of Nrg-1-expressing neurons.
Assuntos
Membrana Celular/metabolismo , Sobrevivência Celular/fisiologia , Neuregulina-1/metabolismo , Neurônios Aferentes/metabolismo , Transdução de Sinais/fisiologia , Animais , Apoptose/fisiologia , Comunicação Celular/fisiologia , Membrana Celular/ultraestrutura , Células Cultivadas , Receptores ErbB/metabolismo , Potenciais da Membrana/fisiologia , Camundongos , Microscopia Eletrônica , Neurônios Aferentes/ultraestrutura , Peptídeo Hidrolases/metabolismo , Estrutura Terciária de Proteína/fisiologia , Transporte Proteico/fisiologia , Gânglio Espiral da Cóclea/metabolismo , Gânglio Espiral da Cóclea/ultraestruturaRESUMO
Neuronal nicotinic receptors (nAChRs) appear to function at both pre- and postsynaptic sites, to modulate the release of neurotransmitter, and to mediate synaptic transmission, respectively. Localization of functional nAChRs at presynaptic structures has only been possible under the best of circumstances where the presynaptic structure is very large allowing direct nAChR channel recording. We report here a novel and simple method that allows the visualization of stimulus-evoked changes in Fura-2 fluorescence in the presynaptic structures of essentially any neuron type in vitro. Following 'loading' of all neurons by incubation with the calcium-sensitive dye, Fura-2-AM, we selectively reduced the fluorescent signal in the postsynaptic neuron by injecting the Fura-2 quenching agent, Mn(2+), into the postsynaptic neuron. After quenching, nicotine treatment elicits calcium transients that can be observed in spatially distinct regions of neurite bundles contacting the Mn(2+)-infused neuron. Thus, the approach described allows one to readily map the distribution of activated nAChRs on presynaptic inputs in vitro.
Assuntos
Cálcio/metabolismo , Microscopia de Fluorescência/métodos , Neurônios Motores/citologia , Neurônios Motores/metabolismo , Receptores Nicotínicos/metabolismo , Receptores Pré-Sinápticos/metabolismo , Animais , Cálcio/análise , Sinalização do Cálcio/efeitos dos fármacos , Sinalização do Cálcio/fisiologia , Células Cultivadas , Embrião de Galinha , Fura-2 , Gânglios Simpáticos , Região Lombossacral , Nicotina/administração & dosagem , Receptores Nicotínicos/análise , Receptores Pré-Sinápticos/análise , Reprodutibilidade dos Testes , Sensibilidade e Especificidade , Sinapses/fisiologia , Transmissão Sináptica/efeitos dos fármacos , Transmissão Sináptica/fisiologiaRESUMO
It is well known, although not well understood, that smoking and eating just do not go together. Smoking is associated with decreased food intake and lower body weight. Nicotine, administered either by smoking or by smokeless routes, is considered the major appetite-suppressing component of tobacco. Perhaps the most renowned example of nicotine's influence on appetite and feeding behavior is the significant weight gain associated with smoking cessation. This article presents an overview of the literature at, or near, the interface of nicotinic receptors and appetite regulation. We first consider some of the possible sites of nicotine's action along the complex network of neural and non-neural regulators of feeding. We then present the hypothesis that the lateral hypothalamus is a particularly important locus of the anorectic effects of nicotine. Finally, we discuss the potential role of endogenous cholinergic systems in motivational feeding, focusing on cholinergic pathways in the lateral hypothalamus.
Assuntos
Regulação do Apetite/efeitos dos fármacos , Regulação do Apetite/fisiologia , Ingestão de Alimentos/efeitos dos fármacos , Hipotálamo/efeitos dos fármacos , Nicotina/farmacologia , Animais , Ingestão de Alimentos/fisiologia , Humanos , Receptores Nicotínicos/metabolismo , FumarRESUMO
The lateral hypothalamus (LH) is an important center for the integration of autonomic and limbic information and is implicated in the modulation of visceral motor and sensory pathways, including those underlying feeding and arousal behaviors. LH neurons in vitro release both ATP and GABA. The control of ATP and GABA co-transmission in LH may underlie the participation of LH in basic aspects of arousal and reinforcement. LH neurons receive cholinergic input from the pedunculopontine and laterodorsal tegmental nuclei as well as from cholinergic interneurons within the LH per se. This study presents evidence for nicotinic acetylcholine receptor (nAChR)-mediated enhancement of GABAergic, but not of purinergic, transmission despite the co-transmission of ATP and GABA at LH synapses in vitro. Facilitation of GABAergic transmission by nicotine is inhibited by antagonists of (alphabeta)*-containing nAChRs, but is unaffected by an alpha7-selective antagonist, consistent with a nAChR-mediated enhancement of GABA release mediated by non-alpha7-containing nAChRs. Activation of muscarinic ACh receptors enhances the release of ATP while concomitantly depressing GABAergic transmission. The independent modulation of ATP/GABAergic transmission may provide a new level of synaptic flexibility in which individual neurons utilize more than one neurotransmitter but retain independent control over their synaptic activity.
Assuntos
Trifosfato de Adenosina/fisiologia , Hipotálamo/fisiologia , Sistema Nervoso Parassimpático/fisiologia , Receptores Purinérgicos/fisiologia , Sinapses/fisiologia , Transmissão Sináptica/fisiologia , Ácido gama-Aminobutírico/fisiologia , Animais , Embrião de Galinha , Colinérgicos/farmacologia , Potenciais Pós-Sinápticos Excitadores/efeitos dos fármacos , Hipotálamo/efeitos dos fármacos , Potenciais da Membrana/fisiologia , Muscarina/farmacologia , Agonistas Muscarínicos/farmacologia , Nicotina/farmacologia , Agonistas Nicotínicos/farmacologia , Sistema Nervoso Parassimpático/efeitos dos fármacos , Técnicas de Patch-Clamp , Receptores Muscarínicos/efeitos dos fármacos , Receptores Purinérgicos/efeitos dos fármacos , Sinapses/efeitos dos fármacos , Transmissão Sináptica/efeitos dos fármacosRESUMO
Autonomic and limbic information is integrated within the lateral hypothalamus (LH), and excitability of LH neurons is important in the control of feeding and behavioral arousal. Despite the prominent expression of P2X-type ATP receptors throughout the hypothalamus, the role of ATP in LH excitability is not known. Perforated-patch-clamp recordings of synaptically coupled neurons from both embryonic chick and postnatal mouse lateral hypothalamus in vitro reveal robust stimulus-evoked purinergic synaptic transmission. Suprathreshold activation elicits reliable and concurrent release of ATP with GABA. Tetrodotoxin-resistant P2X receptor-mediated events are readily observed at LH synapses from the embryonic chick, whereas GABA miniature postsynaptic currents (mPSCs) are recorded in innervated LH neurons from either embryonic chicks or postnatal mice. Two distinct mPSCs are recorded at ATP-GABA cosynapses; one has a monoexponential decay phase and is modulated by flunitrazepam, and the other has a decay phase that is best fit by a sum of two exponential functions (tau(fast) and tau(slow)), and only the tau(slow) component is affected by flunitrazepam. Bicuculline does not completely inhibit all mPSCs. The remaining bicuculline-resistant mPSCs are blocked by suramin, and their decay phase is briefer than that of GABAergic mPSCs. Furthermore, at a holding potential intermediate for the reversal potentials of GABA(A) and P2X receptors, little or no current is observed, consistent with concomitant release (and detection) of GABA and ATP. Together, our data suggest that a subset of spontaneous and evoked PSCs arise from the concurrent activation of both GABA(A) and P2X receptors.