Evaluation of Taenia solium and Taenia crassiceps cysticercal antigens for immunodiagnosis of neurocysticercosis using ELISA on cerebrospinal fluid samples.

The efficacy of whole parasite and vesicular fluid antigen extracts from Taenia solium and Taenia crassiceps cysticerci for immunodiagnosis of neurocysticercosis was evaluated using ELISA on cerebrospinal fluid samples. Anticysticercal IgG antibodies were assayed in cerebrospinal fluid samples from 23 patients with neurocysticercosis and 35 patients with other neurological disorders. The ELISA reaction for the whole Taenia solium cysticercal extract showed 91.3% sensitivity and 94.3% specificity, whereas the sensitivity and specificity of the ELISA for the whole Taenia crassiceps cysticercal extract were 87% and 94.3%, respectively. The ELISA reactions for vesicular fluid from Taenia solium or Taenia crassiceps showed 91.3% sensitivity and 97.1% specificity. Considering the results obtained from the four antigen preparations, vesicular fluid from Taenia solium and Taenia crassiceps cysticerci may be useful as a source of antigens for immunological reactions that are used for detecting specific antibodies in cerebrospinal fluid samples from patients with neurocysticercosis.

Evaluation of Taenia solium and Taenia crassiceps cysticercal antigens for immunodiagnosis of neurocysticercosis using ELISA on cerebrospinal fluid samples

The efficacy of whole parasite and vesicular fluid antigen extracts from Taenia solium and Taenia crassiceps cysticerci for immunodiagnosis of neurocysticercosis was evaluated using ELISA on cerebrospinal fluid samples. Anticysticercal IgG antibodies were assayed in cerebrospinal fluid samples from 23 patients with neurocysticercosis and 35 patients with other neurological disorders. The ELISA reaction for the whole Taenia solium cysticercal extract showed 91.3 percent sensitivity and 94.3 percent specificity, whereas the sensitivity and specificity of the ELISA for the whole Taenia crassiceps cysticercal extract were 87 percent and 94.3 percent, respectively. The ELISA reactions for vesicular fluid from Taenia solium or Taenia crassiceps showed 91.3 percent sensitivity and 97.1 percent specificity. Considering the results obtained from the four antigen preparations, vesicular fluid from Taenia solium and Taenia crassiceps cysticerci may be useful as a source of antigens for immunological reactions that are used for detecting specific antibodies in cerebrospinal fluid samples from patients with neurocysticercosis.

A eficácia de extratos antigênicos de parasitas totais e líquido vesicular de cisticercos de Taenia solium e Taenia crassiceps para o imunodiagnóstico da neurocisticercose foi avaliada por meio de reações de ELISA em amostras de líquido cefalorraquidiano. Anticorpos IgG anti-cisticercos foram pesquisados em amostras de líquido cefalorraquidiano de 23 pacientes com neurocisticercose e 35 pacientes com outras doenças neurológicas. A reação ELISA com o extrato bruto total de cisticercos de Taenia solium apresentou 91,3 por cento de sensibilidade e 94,3 por cento de especificidade, enquanto a sensibilidade e a especificidade da reação ELISA com o extrato total de cisticercos de Taenia crassiceps foram 87 por cento e 94,3 por cento, respectivamente. As reações ELISA com o líquido vesicular de Taenia solium ou Taenia crassiceps mostraram 91,3 por cento de sensibilidade e 97,1 por cento de especificidade. Considerando os resultados obtidos com as quatro preparações antigênicas, o liquido vesicular de cisticercos de Taenia solium e Taenia crassiceps pode ser útil como fonte de antígenos em reações imunológicas usadas para detectar anticorpos específicos em amostras de líquido cefalorraquidiano de pacientes com neurocisticercose.