RESUMO
Given its unusual lymphatic drainage system, the tonsil is a rare site of metastasis, with few reports in the human and veterinary literature. Prognosis in cases of tonsillar metastasis is reportedly poor. We describe here a unique case of urinary bladder urothelial carcinoma (UC) with metastasis to the tonsil in an 11-y-old, spayed female, mixed-breed dog. At presentation, the patient had a history of a growing neck mass and increasing lethargy, hyporexia, weight loss, drooling, and diarrhea for 2 wk. A carcinoma was diagnosed by cytology. Given the poor prognosis, the patient was euthanized. Postmortem examination revealed masses in the inguinal region, cranioventral neck region including tonsil, and urinary bladder. Histologically, the masses were composed of large polyhedral cells arranged in dense sheets and nests with occasional large, clear, intracytoplasmic vacuoles. Neoplastic cells were multifocally positive for uroplakin III and cytokeratin 8/18 by immunohistochemistry. UC with metastasis to tonsil and lymph nodes was diagnosed.
Assuntos
Carcinoma de Células de Transição , Doenças do Cão , Neoplasias de Cabeça e Pescoço , Neoplasias do Sistema Respiratório , Neoplasias da Bexiga Urinária , Animais , Carcinoma de Células de Transição/diagnóstico , Carcinoma de Células de Transição/patologia , Carcinoma de Células de Transição/veterinária , Doenças do Cão/diagnóstico , Doenças do Cão/patologia , Cães , Feminino , Neoplasias de Cabeça e Pescoço/patologia , Neoplasias de Cabeça e Pescoço/veterinária , Humanos , Linfonodos/patologia , Masculino , Prognóstico , Neoplasias do Sistema Respiratório/veterinária , Neoplasias da Bexiga Urinária/patologia , Neoplasias da Bexiga Urinária/veterináriaRESUMO
Histoplasmosis is one of the most common systemic fungal diseases in cats from the United States. It commonly causes respiratory or disseminated disease and is often associated with one or more cytopenias. Here, we describe 32 cats in which a Histoplasma-like fungal infection was associated with concurrent hemophagia in at least one sample site, commonly spleen, bone marrow, liver, and/or lymph node. The degree of hemophagia was characterized as moderate or marked in the majority of cases, and in all cases, there was a predominance of phagocytized mature erythrocytes. A few cases also had macrophages with phagocytized erythroid precursors, platelets, and/or neutrophils. Complete blood count results were available for 25 cats, and cytopenias were common (20/25), including solitary anemia (10), anemia and thrombocytopenia (5), solitary neutropenia (2), pancytopenia (2), and anemia and neutropenia (1). Bone marrow samples were only available in a small subset of cases, preventing the further assessment of the causes of the cytopenias. Hemophagocytosis has been previously reported in cats with neoplastic diseases and a cat with calicivirus infection, and likely occurs with other conditions as well, such as hemorrhage or hemolysis. Results of this report suggest that systemic fungal disease is an additional differential to consider when there is hemophagia in a feline cytology sample.
Assuntos
Doenças do Gato/diagnóstico , Histoplasmose/veterinária , Micoses/veterinária , Animais , Biópsia por Agulha Fina/veterinária , Contagem de Células Sanguíneas/veterinária , Medula Óssea/patologia , Doenças do Gato/microbiologia , Doenças do Gato/patologia , Gatos , Eritrócitos/patologia , Feminino , Histoplasmose/diagnóstico , Histoplasmose/microbiologia , Histoplasmose/patologia , Fígado/patologia , Linfonodos/patologia , Macrófagos/patologia , Masculino , Micoses/diagnóstico , Micoses/microbiologia , Micoses/patologia , Fagocitose , Baço/patologiaAssuntos
Doenças do Cão/patologia , Granuloma de Corpo Estranho/veterinária , Suturas/veterinária , Animais , Biópsia por Agulha/veterinária , Cães , Células Epitelioides/patologia , Células Gigantes/patologia , Granuloma de Corpo Estranho/patologia , Macrófagos/patologia , Ombro/patologia , Ombro/cirurgiaAssuntos
Doenças do Cão/diagnóstico , Neoplasias Renais/veterinária , Neoplasias Hepáticas/veterinária , Sarcoma/veterinária , Animais , Biópsia por Agulha/veterinária , Doenças do Cão/patologia , Cães , Rim/patologia , Neoplasias Renais/diagnóstico , Neoplasias Renais/patologia , Fígado/patologia , Neoplasias Hepáticas/diagnóstico , Neoplasias Hepáticas/patologia , Masculino , Sarcoma/diagnóstico , Sarcoma/patologiaAssuntos
Doenças do Cão/diagnóstico , Mastócitos , Neoplasias Nasais/veterinária , Neoplasias Faríngeas/veterinária , Animais , Doenças do Cão/patologia , Cães , Epistaxe/complicações , Epistaxe/veterinária , Feminino , Neoplasias Nasais/diagnóstico , Neoplasias Nasais/patologia , Neoplasias Nasais/fisiopatologia , Neoplasias Faríngeas/diagnóstico , Neoplasias Faríngeas/patologia , Neoplasias Faríngeas/fisiopatologiaRESUMO
A 21-year-old American Saddlebred mare died with a history of weight loss and breathing difficulties of 1 month duration. Post-mortem examination revealed a copious pleural effusion with multifocal to coalescing numerous white to grey nodular masses on the serosal surface of the pericardium, lungs and thoracic cavity. In addition, the left thyroid gland was markedly enlarged. A thoracic mesothelioma and C-cell adenoma with amyloid deposits of the left thyroid gland were diagnosed by histopathology and confirmed by immunohistochemistry employing antibodies against cytokeratin (CK), vimentin and calcitonin. Amyloid deposits in the thyroid tumour were confirmed by Congo red staining with apple-green birefringence under polarized light. Mesothelioma remains an uncommon neoplasm encountered in aged horses. Discussion includes the diagnostic challenge of differentiating carcinomatosis from mesothelioma by histology and differentiating reactive and neoplastic mesothelial cells by cytology.
RESUMO
OBJECTIVE To compare percentages of mast cells in lymph node (LN) aspirate samples from clinically normal dogs, dogs with allergic dermatologic disease (ADD), and dogs with cutaneous mast cell tumors (MCTs). DESIGN Prospective cross-sectional study. ANIMALS 20 healthy dogs (group 1), 20 dogs with ADD (group 2), and 20 dogs with an MCT on the head or limbs (group 3). PROCEDURES LN aspirate samples were obtained from easily accessible LNs in group 1, affected skin regions in group 2, and the likely draining LN or LNs of the MCT in group 3; the percentage of mast cells was manually determined for each LN. For group 3, LNs were cytologically categorized with a modified version of a published metastasis categorization scheme. RESULTS Median (range) percentage of mast cells in aspirate samples was 0% (0% to 0.1%) for group 1, 0.05% (0% to 0.55%) for group 2, and 0.4% (0% to 77.4%) for group 3. In group 3, 16 LNs (13 dogs) were palpably normal in size; 6 of these had evidence of possible or certain metastasis. Seven LNs (7 dogs) in group 3 were palpably enlarged, and 5 of these had evidence of certain metastasis. CONCLUSIONS AND CLINICAL RELEVANCE This study provided evidence to support the use of a uniform cytologic grading system to further define nodal metastasis in dogs with MCTs as well as estimates of the percentage of mast cells in LN aspirate samples for healthy dogs and dogs with ADD. Palpably normal LNs in dogs with cutaneous MCT may contain metastasis.
Assuntos
Dermatite/veterinária , Doenças do Cão/patologia , Linfonodos/citologia , Mastócitos/citologia , Mastocitoma/veterinária , Neoplasias Cutâneas/veterinária , Animais , Estudos Transversais , Dermatite/patologia , Cães , Feminino , Masculino , Mastocitoma/patologia , Estudos Prospectivos , Neoplasias Cutâneas/patologiaAssuntos
Doenças do Gato/diagnóstico , Pancreatopatias/veterinária , Infecções por Trematódeos/veterinária , Animais , Biópsia por Agulha Fina/veterinária , Doenças do Gato/parasitologia , Doenças do Gato/patologia , Gatos , Feminino , Pâncreas/parasitologia , Pancreatopatias/parasitologia , Infecções por Trematódeos/diagnóstico , Infecções por Trematódeos/patologiaAssuntos
Antibacterianos/uso terapêutico , Infecções por Bordetella/veterinária , Bordetella bronchiseptica/isolamento & purificação , Cloranfenicol/uso terapêutico , Doenças dos Cavalos/diagnóstico , Pneumonia Bacteriana/veterinária , Animais , Antibacterianos/farmacologia , Infecções por Bordetella/diagnóstico , Infecções por Bordetella/tratamento farmacológico , Bordetella bronchiseptica/efeitos dos fármacos , Cloranfenicol/farmacologia , Cílios/microbiologia , Células Epiteliais/microbiologia , Doenças dos Cavalos/tratamento farmacológico , Doenças dos Cavalos/microbiologia , Cavalos , Masculino , Pneumonia Bacteriana/diagnóstico , Pneumonia Bacteriana/tratamento farmacológicoAssuntos
Biópsia por Agulha Fina/veterinária , Doenças do Cão/diagnóstico , Tecido Adiposo/patologia , Animais , Diagnóstico Diferencial , Doenças do Cão/patologia , Cães , Lipoma/diagnóstico , Lipoma/patologia , Lipoma/veterinária , Linfonodos/patologia , Neoplasias Cutâneas/diagnóstico , Neoplasias Cutâneas/patologia , Neoplasias Cutâneas/veterináriaRESUMO
The Han:SRPD-cy rat is a well-recognized model of human autosomal-dominant polycystic kidney disease. The disease is characterized by the development of progressive renal cysts, leading to declining renal function. Disease progression typically is monitored by measurement of plasma urea concentration. Although plasma urea may be an adequate measure of overall renal function, urinary biomarkers capable of accurately monitoring disease progression may be equally useful. The goal of this study was to assess several urinary biomarkers as potential markers of disease progression in male and female Han:SPRD-cy rats. These biomarkers were compared with changes in plasma urea concentration and morphometric changes as the disease progressed. Urinary activity of N-acetyl-ß-D-glucosaminidase and concentration of α-glutathione S-transferase were measured as markers of proximal tubular dysfunction, glutathione S-transferase Yb1 as a distal tubular marker, and collagen IV as a biomarker for glomerular lesions. Urinary albumin was used as biomarker of glomerular or proximal tubular lesions. Albuminuria increased in male rats as the disease progressed, correlating with increasing plasma urea and morphologic changes. Urine concentrations of α-glutathione S-transferase decreased significantly in the male heterozygotic compared with wildtype rats in the later stages of the disease. Urinary concentrations of glutathione S-transferase Yb1 and collagen IV and activity of N-acetyl-ß-D-glucosaminidase did not change during disease progression. Measurement of urinary albumin and concentrations of α-glutathione S-transferase may be useful for monitoring disease progression in the male Han:SPRD-cy rat model in future experiments.
Assuntos
Biomarcadores/urina , Progressão da Doença , Doenças Renais Policísticas/diagnóstico , Acetilglucosaminidase/urina , Albuminúria/diagnóstico , Animais , Colágeno Tipo IV/urina , Creatinina/urina , Feminino , Taxa de Filtração Glomerular , Glutationa Transferase/urina , Isoenzimas/urina , Rim/metabolismo , Rim/patologia , Masculino , Doenças Renais Policísticas/genética , Doenças Renais Policísticas/patologia , Proteinúria/diagnóstico , Ratos , Ratos Endogâmicos , Ureia/sangueRESUMO
OBJECTIVE: To describe the cytologic and histopathologic appearance of degenerate disk material in dogs with Hansen type I intervertebral disk disease (IVDD). STUDY DESIGN: Case series. ANIMALS: Dogs (n=45) that had surgical intervention for Hansen type I IVDD (January-November 2007). METHODS: Impression smears and histopathologic sections were prepared from surgically removed degenerate disk material. All slides were evaluated for overall cellularity, quantity and attributes of extracellular matrix, types of cells present, and their cytomorphology. Histopathologic sections were also examined for presence of neovascularization and hemorrhage. RESULTS: Cytologically, 11 of 45 samples consisted of only extracellular matrix, 30 had evidence of inflammation, and 20 contained dysplastic spindloid cells. Histologically, hyaline cartilage predominated in 35 of 45 samples, fibrocartilage in 4, and spindloid cells in 6; 37 of 45 were inflamed, 37 were hemorrhagic, and 13 had neovascularization. CONCLUSIONS: The cytologic and histopathologic appearance of extruded degenerate disk material in dogs is variable and can include dysplastic spindloid cells. CLINICAL RELEVANCE: The variability in cytologic findings and frequent presence of dysplastic spindloid cells suggest that cytology alone may not be a reliable tool to differentiate degenerate canine disk material from a mesenchymal neoplasm.