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Background: A significant difference exists between the reported optimal timing of indocyanine green (ICG) injection during fluorescence cholangiography and ICG dissipation time from the serum. There are no reports on alterations in ICG concentration in biliary fluid over time. Herein, we measured the concentration of ICG and the fluorescence intensity ratio between the common bile duct (CBD) and liver, which was recognized as a parameter of the visibility of the CBD. Materials and methods: ICG (0.05 mg/kg) was injected intravenously into female pigs (n = 7). Afterwards, the fluorescence of the CBD and liver was detected at 30 min, 2 h, and 4 h. Biliary fluid was collected from cannulated CBD tubes. The fluorescence intensity was measured using captured images and calculated using the ImageJ image-processing program. ICG concentration was measured using spectrophotometry and compared using an analysis of variance test. Results: Biliary ICG concentrations at 30 min, 2 h, and 4 h were 92.07 ± 27.72 µg/mL, 37.14 ± 9.76 µg/mL (p < 0.05 vs. 30 min), and 13.91 ± 5.71 µg/mL (p < 0.05 vs. 30 min), respectively; p < 0.01. The CBD/liver fluorescence intensity ratios at 30 min, 2 h, and 4 h were 1.25 ± 0.72, 2.39 ± 1.28 (p < 0.05 vs. 30 min and 4 h), and 3.38 ± 1.73 (p < 0.05 vs. 30 min and 2 h), respectively. Conclusions: The ICG biliary concentration was highest at 30 min, whereas the CBD/liver fluorescence intensity ratio was highest at 4 h. Decreasing the fluorescence intensity of the liver may be an important approach for improving the visualization of the CBD during fluorescence cholangiography. Institutional protocol number: PE/EA/491-5/2020.
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The European Union is earmarking more and more resources each cycle to support research, development and innovation, and the projects set up by the working groups are helping to develop innovative solutions to reflecting societal issues of today. Since 2014, the Department of Surgical Research and Techniques of the Cardiovascular Center at Semmelweis University has been participating in international consortia supported by the Erasmus+ programme, which aim to modernise the teaching methodology of surgical training and to integrate IT technology into the training of surgical residents at pan-European level. This paper briefly summarises the international projects in which the Department has been involved over the last 7 years.
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With the continuous development of information technology, robotics and data science will certainly have a similar impact on invasive medicine over the next 20 years as it has had on manufacturing technology in the recent decades. Early image-guided systems and surgical robots were employed in the operating room primarily for their accuracy and reliability, as they allowed for faster and safer interventions with minimal tissue damage, targeting especially orthopedics and neurosurgery. On the other hand, a real global breakthrough came with the teleoperated da Vinci Surgical System, ideal for soft tissue procedures. The success and dominance of the da Vinci has dimmed the dozens of other surgical robots already on the market. It partially originated from the teleoperation concept of Robot-Assisted Minimally Invasive Surgery, where the full control of the robotic tools is always maintained by the human operator via the console. Nevertheless, the availability of data at large brings new possibilities, e.g., the in-view integration of preoperative data, data fusion based on surgical navigation, and error compensation have become increasingly available in prototypes. Surgical decision support and the elimination/eviction of potential errors also became increasingly important in telesurgical applications. Appropriate algorithms for handling distortions, delays, and other, even malicious, interference attempts during communication are essential. The concept of robotic telesurgery originates from NASA, and even as of today they are actively exploring the additional possibilities offered by cutting-edge technology to improve surgical systems using data science methods. In the not so distant future, even soft tissue interventions will be performed by autonomous robots. The aim of this article is to present the reader the basic concepts of this modern interdisciplinary field named Computer-Integrated Surgery, and to introduce the most important robots and robotic systems. We provide an overview of the different forms of telesurgery and describe the idea and the complexity of data-driven interventions.
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Robótica , Humanos , Reprodutibilidade dos TestesRESUMO
Introduction: The diverticulosis of the sigmoid colon may be congenital or acquired. The Giant Colonic Diverticulum (GCD), defined as a diverticulum larger than 4 cm, is a rare, but clinically important entity. The McNutt (1988) classification is used differentiate the three subgroups of giant diverticula. Type 1 diverticula are pulsion pseudo-diverticula, which enlarge gradually. Type 2 are inflammatory diverticula due to a previous subserosal perforation, where the abscess cavity is communicating with the bowel lumen and its wall would be gradually composed of fibrous scar tissue, lacking the intestinal histological layers. Type 3 are the real, congenital diverticula of the colon, containing all layers of the intestinal wall. We present a case of a 59-year-old male patient, who was admitted to our Dept. of Surgery in emergency with the diagnosis of strangulated left inguinal hernia. Abdominal CT demonstrated the herniation of the sigmoid colon into the hernia sac without the signs of bowel obstruction. The patient was operated on urgently; subsequent to a left inguinal incision, a Type 3 giant diverticulum of the sigmoid colon was found in the hernia sac. Laparotomy was performed, and the unusually large, 7-8 cm long strangulated diverticulum was liberated. There were no other pathological findings, the sigmoid colon was not damaged. The diverticulum was resected with a TA stapler, and the staple line was inverted with a layer of seromuscular sutures. The inguinal hernia orifices were reconstructed both intraabdominally and externally. The postoperative course was uneventful, the patient was discharged on the 7th day. The recommendations concerning the diagnosis and treatment GCD are briefly reviewed. Due to the McNutt Type 3 characteristics, we opted for the less invasive, simple diverticulectomy instead of sigmoid resection (Hartmann's procedure). Our case was an extremely rare complication of the already uncommon GCD, resolved by an unusual surgical intervention leading to a successful cure.
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Divertículo , Hérnia Inguinal , Divertículo/diagnóstico , Divertículo/cirurgia , Humanos , Laparotomia , Masculino , Pessoa de Meia-IdadeRESUMO
Introduction: Not only atraumatic surgical technique, precise bleeding control, removal foreign materials from the abdomen, but also avoiding desiccation or mechanical damage of peritoneal surface at abdominal surgery mean today evidence based expectation. Peritoneum with its extensive surface and special histological structure represents an important factor in normal physiological processes, furthermore as "Guard of abdomen" it has an important role to localise inflammatory reactions, useful as dialysing surface and provides also possibility for hyperthermic abdominal chemotherapy in tumour treatment. Largest part of peritoneal sac covers small intestine and colon. To prevent postoperative complications it is necessary to avoid desiccation of intestinal tract at laparoscopic and at open procedures as well consequently "rehyration" is a routine recommendation today. Desiccation of intestinal tract results postoperative adhesions, furthermore damage of serosa will increase permeability of intestine wall and can result perforation. All the surgical recommendations suggest keeping intestine moist, whereas there are only a few real studies in surgical literature to support or to deny this theory. Our study reviews the pathophysiological and surgical respects of this situation and summarizes the results of latest researches of combined functions of peritoneum.
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Laparoscopia , Peritônio , Humanos , Complicações Pós-OperatóriasRESUMO
Due to the fast spread of laparoscopic cholecystectomy, surgical procedures have been changed essentially. The new techniques applied for both abdominal and thoracic procedures provided the possibility for minimally invasive access with all its advantages. Robots - originally developed for industrial applications - were retrofitted for laparoscopic procedures. The currently prevailing robot-assisted surgery is ergonomically more advantageous for the surgeon, as well as for the patient through the more precise preparative activity thanks to the regained 3D vision. The gradual decrease of costs of robotic surgical systems and development of new generations of minimally invasive devices may lead to substantial changes in routine surgical procedures.
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Endoscopia/instrumentação , Robótica/instrumentação , Robótica/métodos , Cirurgia Assistida por Computador/instrumentação , Cirurgia Assistida por Computador/métodos , Equipamentos Cirúrgicos/tendências , Interface Usuário-Computador , Colecistectomia Laparoscópica/instrumentação , Custos Diretos de Serviços/tendências , Endoscopia/métodos , Humanos , Laparoscopia/instrumentação , Cirurgia Endoscópica por Orifício Natural/instrumentação , Robótica/economia , Robótica/tendências , Cirurgia Assistida por Computador/economia , Cirurgia Assistida por Computador/tendências , Instrumentos Cirúrgicos/estatística & dados numéricos , VozRESUMO
INTRODUCTION: The small intestine is one of the most sensitive organs to ischemia-reperfusion injury during transplantation. Cytoprotective effect of pituitary adenylate cyclase-activating polypeptide (PACAP) is well known. The aim of our study was to measure changes of PACAP-38-like immunoreactivities and cytokine levels in intestinal grafts stored PACAP-38 containing preservation solution. MATERIAL AND METHODS: Small-bowel autotransplantation was performed on male Wistar rats (n = 56). Grafts were stored in University of Wisconsin (UW) solution at 4 °C for 1 (GI), 3 (GII), and 6 hours (GIII); and in PACAP-38 containing UW solution for 1 (GIV), 3 (GV), and 6 hours (GVI). Reperfusion lasted 3 hours in each group. Intestinal PACAP-38 immunoreactivities were measured by radioimmunoassay. To measure cytokine from tissue homogenates we used rat cytokine array and Luminex Multiplex Immunoassay. RESULTS: Levels of PACAP-38-like and PACAP-27-like immunoreactivities decreased by preservation time compared to control. This decrease was significant following 6 hours cold storage (p < 0.05). Values remained significantly higher in grafts stored in PACAP-38 containing UW. Expressions of sICAM-1, L-selectin, tissue inhibitor of metalloproteinase-1 were increased in GIII and were decreased in GVI. CONCLUSION: PACAP-38 increased tissue levels of PACAP-38 and PACAP-27, and decreased cytokine expression. This indicates that PACAP-38 has anti-inflammatory and cytoprotective effects in intestinal autotransplantation model.
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Anti-Inflamatórios/farmacologia , Antioxidantes/farmacologia , Citocinas/metabolismo , Citoproteção/efeitos dos fármacos , Sobrevivência de Enxerto/efeitos dos fármacos , Substâncias de Crescimento/farmacologia , Intestino Delgado/transplante , Polipeptídeo Hipofisário Ativador de Adenilato Ciclase/metabolismo , Polipeptídeo Hipofisário Ativador de Adenilato Ciclase/farmacologia , Traumatismo por Reperfusão/prevenção & controle , Adenosina , Alopurinol , Animais , Regulação para Baixo , Glutationa , Insulina , Molécula 1 de Adesão Intercelular/metabolismo , Intestino Delgado/metabolismo , Selectina L/metabolismo , Masculino , Preservação de Órgãos/métodos , Soluções para Preservação de Órgãos , Radioimunoensaio , Rafinose , Ratos , Ratos Wistar , Traumatismo por Reperfusão/etiologia , Fatores de Tempo , Inibidor Tecidual de Metaloproteinase-1/metabolismo , Transplante AutólogoRESUMO
The fast spread of laparoscopic surgery in the surgical community also required introduction of new methods of surgical education of these techniques. Training boxes applied for this reason meant a considerable help. The technique of the virtual reality introduced simulation, which is a new possibility in education. For the first time in the history of surgery we can measure medical students' or residents' dexterity and one can get acquainted with a surgical procedure in the form of "serious games". By application of the up-to-date imaging methods we can plan the movements of the surgeon's hand even before the planned operation, practice and repeating can contribute to the safety of the real procedure. Open surgical procedures can be practiced on plastic phantoms mimicking human anatomy and the use of interactive touch devices and e-learning can also contribute to practical education of surgery.
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Competência Clínica , Simulação por Computador , Instrução por Computador/tendências , Educação de Pós-Graduação em Medicina/métodos , Educação de Pós-Graduação em Medicina/tendências , Aprendizagem , Procedimentos Cirúrgicos Operatórios/educação , Interface Usuário-Computador , Competência Clínica/normas , Instrução por Computador/instrumentação , Instrução por Computador/métodos , Endoscopia/educação , Humanos , Desempenho Psicomotor , Procedimentos Cirúrgicos Operatórios/métodos , Livros de Texto como Assunto , Jogos de VídeoRESUMO
A huge number of factors play a significant role in the process of adhesion formation, like bleeding, the presence of foreign bodies, tissue injury, tissue destruction, ischemia and hypoxia. Adhesions are present in 95% of the cases following abdominal surgery. As a result of adhesions a large number of postoperative complications can occur, such as abdominal pain, bowel motility disturbances and infertility. Hence, it is important to know the precise mechanism of adhesion formation process and establish a suitable animal model to investigate the underlying mechanisms. Molecules which play a part in the process of adhesion formation were collected from the international literature. Male Wistar rats were used to create the adhesion model. Bleeding, implantation of foreign bodies, creation of ischemic areas and tissue destructions were carried out. Within this experiment the tiny bleeding and ischemic areas did not result in adhesion formation. The adhesion formation due to foreign body implantation depends on the type of the materials. Due to the inhibitory mechanism of adhesion formation there was no adhesion detectable due to tiny peritoneal destruction. The most reliable model was the one when gross tissue destruction of the abdominal wall was applied and the resulting bleeding initiated the adhesion formation process. It is also extremely important to know the key participants in the complex process of adhesion formation. This reliable model can help to work out the proper method of prevention.
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Parede Abdominal/fisiopatologia , Doenças Peritoneais/fisiopatologia , Peritônio/fisiopatologia , Aderências Teciduais/fisiopatologia , Animais , Modelos Animais de Doenças , Corpos Estranhos/complicações , Hemorragia/etiologia , Masculino , Doenças Peritoneais/etiologia , Peritônio/cirurgia , Complicações Pós-Operatórias/fisiopatologia , Ratos , Ratos WistarRESUMO
INTRODUCTION: In 1968 R. E. Fear first reported a trocar site hernia (TSH) in his large series on laparoscopy. Currently, the incidence of TSH is estimated to be 0.65-2.80%. Ports ≥10-mm are usually closed, but ports of the 5-mm trocars are always left open, which may lead to herniation. MATERIAL AND METHODS: Authors guided teaching courses for hands-on animal laparoscopic cholecystectomy (LC) operations, where trainees performed LC-s on 60 animals. Two and four weeks following the operations the animals underwent second look laparoscopy to detect adhesion formation. RESULTS: Trocar site herniation was observed, and in 20% of the animals herniation was found. 70% of the hernias were situated in the 5-mm ports and 30% in the 10-mm ports. CONCLUSION: Port sites should be closed to prevent the formation of TSH. Attention should be payed on the closure of 5-mm trocar sites as well.
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Colecistectomia Laparoscópica/efeitos adversos , Colecistectomia Laparoscópica/instrumentação , Hérnia Ventral/etiologia , Hérnia Ventral/prevenção & controle , Suturas , Cicatrização , Animais , Hérnia Ventral/fisiopatologia , Hungria , Incidência , Instrumentos Cirúrgicos/efeitos adversosRESUMO
CASE REPORT: Invasive aspergillosis is a life threatening complication in immune-compromised patients causing lung tissue destruction. Aspergillus empyema requires aggressive multimodality treatment. MATERIAL AND METHOD: We present a case of Aspergillus empyema treated by thoracic and plastic surgery preserving the lung function in an 18 year-old male patient suffering dermatomyositis and treated with steroids for a long time. After open window thoracostomy (OWT) we used pedicled musculus latissimus dorsi (MLD) flap and mobilised the anterior serratus muscle to close the pleural cavity. CONCLUSION: The intrathoracic use of muscle flaps after OWT in case of chronic Aspergillus empyema can preserve the underlying lung tissue. Cooperation of thoracic and plastic surgeons - as in the cases presented - provides an excellent opportunity to treat successfully of otherwise hopeless patients.
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Empiema Pleural/cirurgia , Pulmão/fisiopatologia , Músculo Esquelético/cirurgia , Procedimentos de Cirurgia Plástica/métodos , Aspergilose Pulmonar/cirurgia , Toracostomia , Dermatomiosite/tratamento farmacológico , Empiema Pleural/fisiopatologia , Humanos , Masculino , Aspergilose Pulmonar/etiologia , Aspergilose Pulmonar/fisiopatologia , Testes de Função Respiratória , Esteroides/administração & dosagem , Esteroides/efeitos adversos , Retalhos Cirúrgicos , Toracotomia , Resultado do Tratamento , Adulto JovemRESUMO
Minimally invasive surgery is a popular alternative to open surgical procedures. Laparoscopic surgeries require highly skilled surgeons with solid theoretical background and significant amount of practice. Pelvitrainers or simulators provide a good opportunity for practicing and developing laparoscopic skills. Laparoscopic training of medical students of the Semmelweis University is performed at the Institute of Experimental Surgery and Surgical Techniques on Apollo pelvitrainers. The trainer, the performed exercises and the time limits have to be validated by several measurements. Statistical evaluation of the results provides a possibility for the numerical evaluation of surgical skills as well as validating the usability of the pelvitrainer. In our study we tested the peg transfer exercise in pelvitrainers on four groups with different surgical background and level of expertise, complete novices (50 persons), medical students (326 persons), surgical residents (15), and experienced surgeons (4), respectively. A time limit of 240 s was defined for novices and 100 s for professionals. During the evaluation of the results the average time and the number of errors were calculated. The mean completion time of amateurs was 365.7 ± 130 s (mean ± standard deviation), with 2.57 errors. The performance of medical students was characterized by 159.3 ± 61.1 s average time with 1.21 errors, the completion time of residents was 257.9 ± 75.7 s with 1.13 error points, and 117.2 ± 29.1 s for the surgeons. These data show significant differences between the group, except between the results of medical students and surgeons. We plan to extend this study with the inclusion of more, experienced surgeons.
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Competência Clínica , Simulação por Computador , Laparoscopia/educação , Médicos/estatística & dados numéricos , Estudantes de Medicina/estatística & dados numéricos , Análise e Desempenho de Tarefas , Adulto , Animais , Competência Clínica/normas , Feminino , Humanos , Internato e Residência , Masculino , Pessoa de Meia-Idade , Fatores de TempoAssuntos
Distinções e Prêmios , Endoscopia/história , Sociedades Médicas , Educação Médica/história , Endoscopia/educação , Endoscopia/instrumentação , Endoscopia/métodos , Endoscopia/tendências , Docentes de Medicina/história , História do Século XX , História do Século XXI , Humanos , Hungria , Laparoscopia/história , Faculdades de Medicina/história , Estados UnidosRESUMO
BACKGROUND: The origins of telemedicine date back to the early 1970s, and combined with the concept of minimally invasive surgery, the idea of surgical robotics was born in the late 1980s based on the principle of providing active telepresence to surgeons. Many research projects were initiated, creating a set of instruments for endoscopic telesurgery, while visionary surgeons built networks for telesurgical patient care, demonstrated transcontinental surgery, and performed procedures in weightlessness. Long-distance telesurgery became the testbed for new medical support concepts of space missions. METHODS: This article provides a complete review of the milestone experiments in the field, and describes a feasible concept to extend telemedicine beyond Earth orbit. With a possible foundation of an extraplanetary human outpost either on the Moon or on Mars, space agencies are carefully looking for effective and affordable solutions for life-support and medical care. The major challenges of surgery in weightlessness are also discussed. RESULTS: Teleoperated surgical robots have the potential to shape the future of extreme health care both in space and on Earth. Besides the apparent advantages, there are some serious challenges, primarily the difficulty of latency with teleoperation over long distances. Advanced virtualization and augmented-reality techniques should help human operators to adapt better to the special conditions. To meet safety standards and requirements in space, a three-layered architecture is recommended to provide the highest quality of telepresence technically achievable for provisional exploration missions. CONCLUSION: Surgical robotic technology is an emerging interdisciplinary field, with a great potential impact on many areas of health care, including telemedicine. With the proposed three-layered concept-relying only on currently available technology-effective support of long-distance telesurgery and human space missions are both feasible.