Biosynthesized Cerium Oxide Nanoparticles CeO2NPs: Recent Progress and Medical Applications.

Currently, nanobiotechnology represents a leading research area that primarily focuses on the safe, eco-friendly synthesis of biocompatible metal oxide nanoparticles. Among these, biosynthesized cerium oxide nanoparticles have particularly received attention in medical science as their unique surface chemistry and dual oxidation state make them excellent antioxidants and freeradical scavengers. Currently, plant extracts are widely explored and employed for the biosynthesis of CeO2NPs. Other biological sources such as marine oyster shell extract, egg-white, biopolymers, e.g., chitosan, agarose, alginate, and others, have also been successfully used for the fabrication of CeO2NPs. This review highlights the recent progress in the biosynthesis of CeO2NPs and the investigation of their medical use as biocompatible anticancer, antibacterial, antifungal, antioxidant, antidiabetic, and wound healing agents. Furthermore, prospects associated with the use of biogenic CeO2NPs in developing novel products in the medical sector are also highlighted.

GC-MS Metabolomics profiling and HR-APCI-MS characterization of potential anticancer compounds and antimicrobial activities of extracts from Picrorhiza kurroa roots.

The present study explores pharmacological potential and phytochemicals profiling of Picrorhiza kurroa extracts against mammalian cancer cell lines and pathogenic microbes. Bioactive extracts from roots of Picrorhiza kurroa were recovered in the methanol, 50% aqueous dichloromethane (50 : 50 v/v) and n-hexane. Antimicrobial activity of the bioactive extracts was assessed against selected strains of bacteria and pathogenic fungi. Aqueous dichloromethane extract showed highest zone of growth inhibition (39.06 ± 1.0 mm) towards Staphylococcus aureus bacteria while methanolic extract showed the lowest inhibition (6.3 ± 4.1 mm) to Escherichia coli bacteria. The tested extracts such as methanol and aqueous dichloromethane exhibited higher inhibition antifungal activity against Aspergillus flavus compared to Fusarium oxysporum. As far as cytotoxicity (MTT assay) of the tested extracts is concerned, n-hexane and aqueous dichloromethane extracts were found to be very active against all cancer cell lines (breast cancer MCF7, MDA-MB-231, SKBR3 and ovarian cancer SKOV3). A preliminary phytochemicals profiling was performed in extracts using GC-MS. Several fractions of active extract were separated with HPLC and analyzed using High Resolution Atmospheric Pressure Chemical Ionization Mass Spectrometry (HR-APCI-MS). Two purified compounds (Dihydromikanolide and 1,3-Dicyclohexyl-4-(cyclohexylimino)-2-(cyclohexylethylamino)-3,4-dihydro-1,3-diazetium) were further evaluated for their anticancer activity against ovarian cancer cell line. Our findings depict that all the tested extracts showed considerable anticancer potential through cell viability assays. The purified compound 1 - Dihydromikanolide from methanolic extract was found to be active against ovarian cancer cells and can be explored as a promising nutra-pharmaceutical candidate against ovarian cancer. However, further studies exploring the molecular pathways and in vivo testing are required.

Phytochemical investigation and effective therapeutic potential of plants extracts against breast and ovarian cancer cell lines: compounds from zizyphus mauritiana and triticum aestivum.

ancer is the leading cause of death, accounting for approximately one out of six people dying with this disease worldwide. Among all, the breast and ovarian cancers are top-ranked causes of women mortalities compared to other disorders. Although, there is advancement in technologies, but still, there are unresolved concerns to overcome the global disease burden. Currently, plants are being explored as a natural remedy to cure disorders. This research was planned to explore phytochemicals in methanolic extracts of Zizyphus mauritiana and Triticum aestivum, and their pharmacological activities were studied through Agrobacterium tumefaciens bacteria, in vitro breast cancer cell line and ovarian cancer cell line to find out novel candidates in disease control and prevention. Eleven different types of bioactive compounds were analysed in the tested extracts. The highest crude extracts percentage (75±0.02) was observed with Z. mauritiana. The extracts showed promising cell growth inhibition and tumor initiation inhibition in potato disc assay. MTT assay and Incucytes imaging analysis revealed that Z. mauritiana extract had a higher anticancer potential with 40 ± 0.92 cell viability against breast cancer cells (SKBR3) and 45 ±0.29 against ovarian cancer cells (SKOV3). In conclusion, these extracts could be used as chemotherapeutics owing to their cheapness, and easy availability. While detailed study is required for further purification and characterization of bioactives/target compounds and in-vivo activity confirmations.

Assessment of α-amylase and α-glucosidase inhibitory potential of Citrus reticulata peel extracts in hyperglycemic/hypoglycemic rats.

Diabetes mellitus is a metabolic disorder of carbohydrate metabolism. The management of Diabetes mellitus with phytochemicals is hallmark of this research. Citrus species are known for their health benefits and are used as traditional food in South East Asia. The total phenolic content of peels was analyzed using different solvents, while Gallic acid was used as standard. Both ethanolic, aqueous extracts of Citrus reticulata peel showed good inhibitory activity against amylase (90.67%, 15.33%) and moderate against glucosidase (70.8%, 14.8%), respectively. Sixteen rats were randomly divided into four groups (G1, G2, G3, and G4); G1 is a negative control (water), G4 is a positive control (Acarbose), while other two are experimental groups like G2 (fed with 100 mL and 20 mg/mL in hypoglycemic and hyperglycemic trials) and G3 fed with 200 mL and 40 mg/mL in hypoglycemic and hyperglycemic trials. A significant effect of treatments and value of time was found in hyperglycemic rats. Ethanolic extract showed a significant reduction in blood glucose levels in hypoglycemic (overnight fasting) rats which was comparable to the positive control. These results suggest that C. reticulata peels can contribute as a useful food ingredient as a potential antihyperglycemic agent in managing type 2 diabetes mellitus. In future, C. reticulata peel will be a good candidate for pharmaceutical industry.