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1.
Pathogens ; 10(10)2021 Oct 13.
Artigo em Inglês | MEDLINE | ID: mdl-34684262

RESUMO

We assessed the association between Toxoplasma gondii (T. gondii) infection of the central nervous system and suicide correlates in suicide decedents. Eighty-seven decedents who died by suicide received in a forensic setting for medico-legal autopsies in Mexico City were studied. Two samples of brain (amygdala and prefrontal cortex) from each decedent were examined for detection of T. gondii using immunohistochemistry. Correlates of suicide including a history of previous suicide attempts, co-morbid mental disorder, consumption of alcohol or tobacco, irritability and aggression, economic problems, presence of drugs or alcohol in blood and suicide method were obtained and analyzed for their association with T. gondii infection. T. gondii immunohistochemistry was positive in prefrontal cortex sections in 6 decedents and in an amygdala section in one decedent. Thus, the prevalence of T. gondii infection in brain in suicide victims was 8.0% (7/87). Bivariate and logistic regression analysis of suicide correlates showed that only a history of depression was associated with T. gondii infection of the brain in suicide victims (OR: 12.00; 95% CI: 2.26-63.46; p = 0.003). Our results provide evidence that T. gondii infection in brain is associated with a history of depression in suicide decedents.

2.
J Clin Med Res ; 11(3): 196-201, 2019 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-30834042

RESUMO

BACKGROUND: To determine the association of infection with human papillomavirus (HPV) and the occupation of female sex worker; and the correlation of infection with HPV with sociodemographic, clinical and behavioral characteristics of female sex workers. METHODS: We performed a case-control study of 217 female sex workers and 354 women without sex work in Durango City, Mexico. We determined the prevalence of infection with HPV in cervical samples of women using polymerase chain reaction, and HPV genotypes were determined using line probe assay. Bivariate and multivariate analyses were used to assess the association between the characteristics of women and infection. RESULTS: Twelve (5.5%) of the 217 sex workers, and 10 (2.8%) of the 354 control women were positive for HPV DNA (age-adjusted OR = 1.51; 95% CI: 0.62 - 3.68; P = 0.36). Six (50.0%) of the 12 HPV DNA positive sex workers had infections with high-risk genotypes (16, 31, 33, 35, 51, 58). Seven (70%) of the 10 HPV DNA positive control women had infections with high-risk genotypes (16, 18, 56, 58, and 66). The frequency of high risk genotypes in the control women was equal with that found in the female sex workers (P = 0.41). Logistic regression analysis showed that the variable alcohol consumption was associated with HPV infection (OR = 4.0; 95% CI: 1.0 - 16.0; P = 0.04). CONCLUSIONS: No association between HPV infection and female sex work was found in our setting. High risk HPV genotypes were prevalent among the women studied. Results can be used for the design of preventive measures against HPV infection.

3.
SAGE Open Med ; 6: 2050312118767767, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-29662676

RESUMO

OBJECTIVES: This study aimed to determine the association between infection with Toxoplasma gondii and epilepsy in patients attended to in a public hospital in the northern Mexican city of Durango. METHODS: We performed an age- and gender-matched case-control study of 99 patients suffering from epilepsy and 99 without epilepsy. Sera of participants were analyzed for anti-T. gondii IgG and IgM antibodies using commercially available enzyme-linked immunoassays. Seropositive samples to T. gondii were further analyzed for detection of T. gondii DNA by polymerase chain reaction. RESULTS: Anti-T. gondii IgG antibodies were found in 10 (10.1%) of the 99 cases and in 6 (6.1%) of the 99 controls (odds ratio = 1.74; 95% confidence interval: 0.60-4.99; p = 0.43). High (> 150 IU/mL) levels of anti-T. gondii IgG antibodies were found in 6 of the 99 cases and in 4 of the 99 controls (odds ratio = 1.53; 95% confidence interval: 0.41-5.60; p = 0.74). Anti-T. gondii IgM antibodies were found in 2 of the 10 IgG seropositive cases, and in 2 of the 6 IgG seropositive controls (odds ratio = 0.50; 95% confidence interval: 0.05-4.97; p = 0.60). T. gondii DNA was not found in any of the 10 anti-T. gondii IgG positive patients. Bivariate analysis of IgG seropositivity to T. gondii and International Statistical Classification of Diseases and related Health Problems, 10th Edition codes of epilepsy showed an association between seropositivity and G40.1 code (odds ratio = 22.0; 95% confidence interval: 2.59-186.5; p = 0.008). Logistic regression analysis showed an association between T. gondii infection and consumption of goat meat (odds ratio = 6.5; 95% confidence interval: 1.22-34.64; p = 0.02), unwashed raw vegetables (odds ratio = 26.3; 95% confidence interval: 2.61-265.23; p = 0.006), and tobacco use (odds ratio = 6.2; 95% confidence interval: 1.06-36.66; p = 0.04). CONCLUSIONS: Results suggest that T. gondii infection does not increase the risk of epilepsy in our setting; however, infection might be linked to specific types of epilepsy. Factors associated with T. gondii infection found in this study may aid in the design of preventive measures against toxoplasmosis.

4.
Eur J Microbiol Immunol (Bp) ; 8(4): 155-158, 2018 Dec 23.
Artigo em Inglês | MEDLINE | ID: mdl-30719333

RESUMO

We determined the association between having a history of surgery and the seroreactivity to T. gondii. An age- and gender-matched case-control study of 391 subjects with a history of surgery and 391 subjects without this history was performed. Sera of subjects were analyzed for detection of anti-T. gondii immunoglobulin G (IgG) and M (IgM) antibodies using enzyme-linked immunoassays. Anti-T. gondii IgG antibodies were found in 25 (6.4%) of the 391 cases and in 21 (5.4%) of the 391 controls (odds ratio [OR] = 1.29; 95% confidence interval [CI]: 0.66-2.18; P = 0.54). The frequency of cases with high IgG antibody levels (10/25: 40.0%) was equal to that found in controls (8/21: 38.1%) (OR = 1.08; 95% CI: 0.32-3.56; P = 0.89). Of the 25 anti-T. gondii IgG antibody seropositive cases, 5 (16.0%) were also positive for anti-T. gondii IgM antibodies. Meanwhile, of the 21 anti-T. gondii IgG antibody seropositive controls, 4 (19.0%) were also positive for anti-T gondii IgM antibodies (OR = 0.81; 95% CI: 0.17-3.72; P = 0.80). Logistic regression showed that only the variable "hysterectomy" was associated with T. gondii seropositivity (OR = 4.6; 95% CI: 1.6-13.4; P = 0.005). Results suggest that having a history of surgery is not an important risk factor for infection with T. gondii. However, the link between T. gondii infection and hysterectomy should be further investigated.

5.
Eur J Microbiol Immunol (Bp) ; 6(2): 90-8, 2016 Jun 24.
Artigo em Inglês | MEDLINE | ID: mdl-27429791

RESUMO

Medical certificates are documents that state the health status of a person. This study aimed to determine the seroprevalence and risk factors for Toxoplasma gondii infection in applicants of medical certificates and to investigate seroprevalence association with characteristics of these individuals. We examined 404 applicants in a public health center in Durango City, Mexico for the presence of anti-Toxoplasma IgG and IgM antibodies using enzyme-linked immunoassays. Of the 404 subjects (mean age of 35.94 ± 13.01) studied, 29 (7.2%) were positive for anti-T. gondii IgG antibodies and 9 (31.0%) of them were also positive for anti-T. gondii IgM antibodies. IgG and IgM seropositivities were associated with vision impairment (P = 0.04) and a history of surgery (P = 0.03), respectively. Prevalence of high (>150 IU/ml) IgG antibody levels was associated with hearing impairment (P = 0.03), and histories of lymphadenopathy (P = 0.04) and miscarriages (P = 0.03). Multivariate analysis showed that T. gondii seropositivity was associated with being born out of Durango State (odds ratio [OR] = 4.65; 95% confidence interval [CI]: 1.25-17. 29; P = 0.02) and soil contact (OR = 4.27; 95% CI: 1.71-10.67; P = 0.002) and negatively associated with consumption of sheep meat (OR = 0.12; 95% CI: 0.02-0.65; P = 0.01). These results could be used for the design of optimal preventive measures against toxoplasmosis and its sequelae.

6.
Biomed Res Int ; 2016: 9597276, 2016.
Artigo em Inglês | MEDLINE | ID: mdl-27446960

RESUMO

Toxoplasma gondii (T. gondii) can be transmitted by blood transfusion. We determined the prevalence of T. gondii infection in healthy blood donors in Hermosillo city, Mexico, and the association of infection with T. gondii with the sociodemographic, clinical, and behavioral characteristics of blood donors. Four hundred and eight blood donors who attended two public blood banks in Hermosillo city were examined for anti-T. gondii IgG and IgM antibodies by using enzyme-linked immunoassays. Of the 408 blood donors (mean age 31.77 ± 9.52; range 18-60 years old) studied, 55 (13.5%) were positive for anti-T. gondii IgG antibodies, and 12 (21.8%) of them were also positive for anti-T. gondii IgM antibodies. Multivariate analysis showed that seropositivity to T. gondii was associated with age (OR = 1.74; 95% CI: 1.03-2.94; P = 0.03) and tobacco use (OR = 2.09; 95% CI: 1.02-4.29; P = 0.04). Seropositivity to T. gondii was correlated with the number of pregnancies, deliveries, and cesarean sections. The seroprevalence of T. gondii infection in blood donors in Sonora is the highest reported in blood donors in northern Mexico so far. This is the first report of an association of T. gondii exposure and tobacco use. Further research to confirm this association is needed.


Assuntos
Doadores de Sangue/estatística & dados numéricos , Toxoplasma/imunologia , Toxoplasmose/sangue , Toxoplasmose/epidemiologia , Adolescente , Adulto , Distribuição por Idade , Anticorpos Antiprotozoários/sangue , Anticorpos Antiprotozoários/imunologia , Estudos Transversais , Feminino , Humanos , Incidência , Masculino , México/epidemiologia , Pessoa de Meia-Idade , Fatores de Risco , Estudos Soroepidemiológicos , Distribuição por Sexo , Toxoplasmose/imunologia , Adulto Jovem
7.
BMJ Open ; 6(5): e010218, 2016 05 12.
Artigo em Inglês | MEDLINE | ID: mdl-27173808

RESUMO

OBJECTIVES: We sought to determine the prevalence of anti-Toxoplasma gondii antibodies in Yoremes and to identify associations of T. gondii exposure with sociodemographic, clinical and behavioural characteristics of Yoremes. DESIGN: A cross-sectional survey. SETTING: Yoremes were enrolled in the locality of Tierra Blanca in the municipality of Navojoa in Sonora State, Mexico. PARTICIPANTS: We studied 200 Yoremes (Mayos); they are an indigenous ethnic group living in a coastal region in northwestern Mexico. PRIMARY AND SECONDARY OUTCOME MEASURES: We assessed the prevalence of anti-Toxoplasma IgG and IgM antibodies in participants using enzyme-linked immunoassays. We used a standardised questionnaire to obtain the characteristics of Yoremes. The association of T. gondii exposure and Yoremes' characteristics was assessed by bivariate and multivariate analyses. RESULTS: Of the 200 Yoremes studied (mean age: 31.50±18.43 years), 26 (13.0%) were positive for anti-T. gondii IgG antibodies and 19 (73.1%) of them were also positive for anti-T. gondii IgM antibodies. Seroprevalence of T. gondii infection did not vary with sex, educational level, occupation or socioeconomic status. In contrast, multivariate analysis of sociodemographic and behavioural characteristics showed that T. gondii exposure was associated with increasing age (OR=1.02; 95% CI 1.00 to 1.04; p=0.03) and consumption of squirrel meat (OR=4.99; 95% CI 1.07 to 23.31; p=0.04). Furthermore, seroprevalence of T. gondii infection was significantly higher in Yoremes with a history of lymphadenopathy (p=0.03) and those suffering from frequent abdominal pain (p=0.03). In women, T. gondii exposure was associated with a history of caesarean sections (p=0.03) and miscarriages (p=0.02). CONCLUSIONS: We demonstrate, for the first time, serological evidence of T. gondii exposure among Yoremes in Mexico. Results suggest that infection with T. gondii might be affecting the health of Yoremes. Results may be useful for an optimal design of preventive measures against T. gondii infection.


Assuntos
Indígena Americano ou Nativo do Alasca , Anticorpos Antiprotozoários/sangue , Carne , Toxoplasma/imunologia , Toxoplasmose/sangue , Toxoplasmose/etnologia , Dor Abdominal/epidemiologia , Dor Abdominal/etnologia , Aborto Espontâneo/epidemiologia , Adolescente , Adulto , Fatores Etários , Animais , Cesárea/estatística & dados numéricos , Estudos Transversais , Feminino , Humanos , Imunoglobulina G/sangue , Imunoglobulina M/sangue , Linfadenopatia/epidemiologia , Masculino , México/epidemiologia , Pessoa de Meia-Idade , Prevalência , Fatores de Risco , Sciuridae , Estudos Soroepidemiológicos , Toxoplasmose/epidemiologia , Adulto Jovem
8.
Eur J Microbiol Immunol (Bp) ; 5(2): 143-9, 2015 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-26185682

RESUMO

The presence of tissue cysts of Toxoplasma gondii has only poorly been investigated in autopsy series. We determined the presence of T. gondii cysts in a series of 51 autopsies in a public hospital using immunohistochemistry of brain and heart tissues. The association of tissue cysts with the general characteristics of the autopsy cases was also investigated. Of the 51 cases studied, five (9.8%) were positive by immunohistochemistry for T. gondii cysts in the brain. None of the heart specimens was positive for T. gondii cysts. The presence of T. gondii cysts in brains did not vary with age, sex, birthplace, residence, education, occupation, or the presence of pathology in the brain. In contrast, multivariate analysis showed that the presence of T. gondii cysts was associated with undernourishment (OR = 33.90; 95% CI: 2.82-406.32; P = 0.005). We demonstrated cerebral T. gondii cysts in an autopsy series in Durango City, Mexico. Results suggest that T. gondii can be more readily found in brain than in heart of infected individuals. This is the first report of an association between the presence of T. gondii in brains and undernourishment.

9.
BMC Infect Dis ; 15: 172, 2015 Apr 03.
Artigo em Inglês | MEDLINE | ID: mdl-25886154

RESUMO

BACKGROUND: Toxoplasma gondii infection has been associated with psychiatric diseases. However, there is no information about the link between this infection and patients with mental and behavioral disorders due to psychoactive substance use. METHODS: We performed a case-control study with 149 psychiatric patients suffering from mental and behavioral disorders due to psychoactive substance use and 149 age- and gender-matched control subjects of the general population. We searched for anti-T. gondii IgG and IgM antibodies in the sera of participants by means of commercially available enzyme-linked immunoassays. Seroprevalence association with socio-demographic, clinical and behavioral characteristics in psychiatric patients was also investigated. RESULTS: Anti-T. gondii IgG antibodies were present in 15 (10.1%) of 149 cases and in 14 (9.4%) of 149 controls (P=1.0). Anti-T. gondii IgM antibodies were found in 11 (7.4%) of the 149 cases and in 16 (10.7%) of the 149 controls (P=0.31). No association of T. gondii exposure with socio-demographic characteristics of patients was found. Multivariate analysis of clinical and behavioral characteristics of cases showed that T. gondii seropositivity was positively associated with consumption of opossum meat (OR=10.78; 95% CI: 2.16-53.81; P=0.003) and soil flooring at home (OR=11.15; 95% CI: 1.58-78.92; P=0.01), and negatively associated with suicidal ideation (OR=0.17; 95% CI: 0.05-0.64; P=0.008). CONCLUSIONS: Mental and behavioral disorders due to psychoactive substance use do not appear to represent an increased risk for T. gondii exposure. This is the first report of a positive association of T. gondii exposure with consumption of opossum meat. Further studies to elucidate the role of T. gondii infection in suicidal ideation and behavior are needed to develop optimal strategies for the prevention of infection with T. gondii.


Assuntos
Anticorpos Antiprotozoários/imunologia , Transtornos Mentais/epidemiologia , Psicotrópicos/efeitos adversos , Toxoplasma/imunologia , Toxoplasmose/epidemiologia , Adolescente , Adulto , Idoso , Animais , Estudos de Casos e Controles , Ensaio de Imunoadsorção Enzimática , Feminino , Humanos , Técnicas Imunoenzimáticas , Imunoglobulina G/imunologia , Imunoglobulina M/imunologia , Masculino , Carne/parasitologia , Transtornos Mentais/induzido quimicamente , Transtornos Mentais/parasitologia , México/epidemiologia , Pessoa de Meia-Idade , Análise Multivariada , Gambás/parasitologia , Estudos Soroepidemiológicos , Toxoplasmose/imunologia , Adulto Jovem
10.
Parasit Vectors ; 8: 77, 2015 Feb 05.
Artigo em Inglês | MEDLINE | ID: mdl-25651797

RESUMO

BACKGROUND: Infection with Toxoplasma gondii can be acquired via the ingestion of undercooked or raw meat containing tissue cysts, or via ingestion of water contaminated with oocysts. Professional long distance truck driving may have epidemiological importance for food-borne infections since drivers eat out of home and in places where hygiene and cooking practices are uncertain. We aimed to determine whether interstate truck drivers in Durango, Mexico have an increased risk of infection with T. gondii as indicated by seropositivity; and to determine the socio-demographic, work, clinical, and behavioral characteristics associated with T. gondii seropositivity in interstate truck drivers. METHODS: Through a case-control study design, 192 truck drivers and 192 controls from the general population of the same region matched by gender and age were examined with enzyme-linked immunoassays for the presence of anti-Toxoplasma IgG and IgM antibodies. Socio-demographic, work, clinical and behavioral characteristics from the truck drivers were obtained. RESULTS: Anti-T. gondii IgG antibodies were found in 23 (12.0%) of 192 truck drivers and in 13 (6.8%) of 192 controls (OR = 21.0; 95% CI: 1.23-358.38; P = 0.002). Anti-T. gondii IgM antibodies were found in 7 (3.6%) cases and in 7 (3.6%) controls (P = 1.00). The seroprevalence of T. gondii infection was higher in drivers with reflex impairment than in those without this impairment (4/13, 30.8% vs 19/179, 10.6%, respectively; P = 0.05), and in drivers with hearing impairment than in those without this impairment (3/7, 42.9% vs 20/185, 10.8%, respectively; P = 0.03). Multivariate analysis of work and behavioral characteristics of truck drives showed positive associations of T. gondii exposure with trips to the south of Mexico (OR = 3.11; 95% CI: 1.02-9.44; P = 0.04) and consumption of horse meat (OR = 5.18; 95% CI: 1.62-16.55; P = 0.005). CONCLUSIONS: Results suggest that interstate truck drivers may have an increased risk for T. gondii infection, and that T. gondii exposure may be impacting neurological functions in truck drivers. Contributing factors for T. gondii exposure should be taken into account for the design of optimal prevention measures against T. gondii infection.


Assuntos
Doenças Profissionais/epidemiologia , Toxoplasma/imunologia , Toxoplasmose/epidemiologia , Adulto , Idoso , Anticorpos Antiprotozoários/sangue , Condução de Veículo , Estudos de Casos e Controles , Feminino , Humanos , Higiene , Imunoglobulina G/sangue , Masculino , México , Pessoa de Meia-Idade , Doenças Profissionais/sangue , Doenças Profissionais/parasitologia , Ocupações/estatística & dados numéricos , Fatores de Risco , Estudos Soroepidemiológicos , Toxoplasma/isolamento & purificação , Toxoplasmose/sangue , Toxoplasmose/parasitologia , Adulto Jovem
11.
Eur J Microbiol Immunol (Bp) ; 4(4): 184-92, 2014 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-25544890

RESUMO

The association of infection with Toxoplasma gondii and occupational exposure to animals has been scantly determined. We performed a case-control study with 200 subjects from Durango Province, Mexico, occupationally exposed to animals and 200 age- and gender-matched subjects without this occupation. Sera from all participants were analyzed for anti-T. gondii IgG and IgM antibodies using enzyme-linked immunoassays. The association of seroprevalence with sociodemographic, work, clinical, and behavioral characteristics in cases was determined. Cases and controls had similar frequencies of anti-T. gondii IgG antibodies (12/200: 6.0% and 11/200: 5.5%, respectively) (OR = 3.0; 95% CI: 0.12-73.64; P = 1.0). The frequency of sera with high (>150 IU/ml) levels of anti-T. gondii IgG antibodies was comparable among cases and controls (P = 0.61). Seroprevalence of anti-T. gondii IgM antibodies was similar in cases (4, 2.0%) than in controls (4, 2.0%) (P = 1.0). Multivariate analysis showed that seropositivity was associated with eating while working (OR = 7.14; 95% CI: 1.91-26.72; P = 0.003) and consumption of duck meat (OR = 5.43; 95% CI: 1.43-20.54; P = 0.01). No association between seropositivity to T. gondii and occupational exposure to animals was found. However, risk factors for infection found should be taken into account to reduce the exposure to T. gondii.

12.
Ginecol Obstet Mex ; 81(2): 77-85, 2013 Feb.
Artigo em Espanhol | MEDLINE | ID: mdl-23596729

RESUMO

BACKGROUND: Human papillomavirus (HPV) causes one of the most common sexually transmitted infections. The causal relationship between HPV and cervical cancer is one of the most important advances in the field of the prevention of this disease. The Food and Drug Administration recently approved two vaccines to prevent HPV infection. OBJECTIVE: To assess the degree of knowledge about HPV and the acceptance of the vaccine against these viruses in mothers of 9 to 15 years old female students from the city of Durango, Mexico. MATERIAL AND METHOD: A prospective, observational, cross-sectional and descriptive study of 470 mothers. The selection was made through random sampling stratified by age and socio-economic status of students from public and private schools in the city of Durango. We applied a questionnaire of 60 questions, 12 open and 48 closed. Groups of acceptance of the vaccine were compared by Student's t and chi2, depending on the type of variable. Reasons for prevalence of non-acceptance and its 95% of risk confidence interval were estimated. RESULTS: The 94% of the women surveyed knew about the vaccine. The 89% would accept vaccination of their daughters if it was free of charge, but only 40% would be willing to buy it; 88% considered that was important to their daughters to receive extensive information about the vaccine from nine years of age. The risk factors identified in this study to not allow vaccination were: not knowing the existence of the vaccine, which increased this risk 3.5 times; not having comprehensive information of the sexually transmitted diseases, which increased it by 2.49 times; and the fear to initiate their sexual life at younger age, which raised it 4.58 times. No difference was found in acceptance to the vaccine for religious or socio-economic reasons; 9% of mothers did not accept the vaccine despite of knowing its existence and the role of HPV in the genesis of cervical cancer. CONCLUSION: The vast majority of surveyed mothers accepted vaccination. The degree of knowledge about the vaccine is an important factor for its acceptance.


Assuntos
Conhecimentos, Atitudes e Prática em Saúde , Mães , Infecções por Papillomavirus/prevenção & controle , Vacinas contra Papillomavirus , Aceitação pelo Paciente de Cuidados de Saúde , Adolescente , Adulto , Criança , Estudos Transversais , Feminino , Humanos , México , Estudos Prospectivos , Saúde da População Urbana
13.
Ginecol Obstet Mex ; 78(5): 261-7, 2010 May.
Artigo em Espanhol | MEDLINE | ID: mdl-20939236

RESUMO

BACKGROUND: In Mexico, cervical cancer is the second leading cause of death in women after breast cancer. The human papillomavirus is associated with intraepithelial lesions, detected up to 99.7% of cervical carcinomas. Despite being easy to detect is a condition that many women suffer. OBJECTIVE: To determine the diagnostic utility of the visual inspection with acetic acid of the uterine cervix compared with the cervical cytology. PATIENTS AND METHOD: Study of diagnostic tests. The study was realized in the Centro de Atención Materno Infantil y Planificación Familiar of the Instituto de Investigación Científica, Durango, Mexico, research of the Juárez University of the State of Durango, from August 23, 2005 to November 13, 2006. 1,521 participants were examined who went consecutively to opportune detection of cervical cancer. One doctor practiced the test of acetic acid and cervical cytology to them, and one digital photograph, which was evaluated by three inter-observers triple blind. Those that was positive to anyone of these tests, were remitted to colposcopy and/or biopsy; also to 10% of selected negative population randomly was realized this procedure. Sensitivity, specificity, positive and negative predictive values and exactitude were determined. For the agreement inter-observer index of Kappa was used. RESULTS: Sensitivity, specificity, values predictive positive, negative and exactitude for the visual inspection with acetic acid were 20, 97, 5 and 99%, respectively. For the cervical cytology were of 80, 99, 57 and 99%, respectively. The force of agreement between the interobservant was poor. CONCLUSIONS: In this study cervical cytology was more useful than visual inspection with acetic acid to detect dysplasias or cervical cancer opportunely, due to detect all the positive true cases confirmed by biopsy.


Assuntos
Ácido Acético , Exame Físico , Neoplasias do Colo do Útero/diagnóstico , Esfregaço Vaginal , Adolescente , Adulto , Biópsia , Colposcopia , Método Duplo-Cego , Feminino , Humanos , Pessoa de Meia-Idade , Variações Dependentes do Observador , Fotografação , Valor Preditivo dos Testes , Sensibilidade e Especificidade , Neoplasias do Colo do Útero/patologia , Adulto Jovem
14.
Ginecol Obstet Mex ; 75(9): 501-8, 2007 Sep.
Artigo em Espanhol | MEDLINE | ID: mdl-18293624

RESUMO

BACKGROUND: The endometrium is a very dynamic organ that experience several half-full processes by the ovarian secretion of estradiol and progesterone. The effect of hormones steroids, in the epithelial cells, endoteliales and estromales of the endometrium, is influenced by receptors of estrogens and progesterone. OBJECTIVE: determine if there are any differences in the density of the estrogen receptors (ER) between the normal endometrial, the simple and complex hyperplasia, the atypical hyperplasia and the. MATERIAL AND METHODS: A retrospective study comparative was made. We included 143 samples that were knit together in 5 categories of the following way: Normal endometrial (n = 38), Simple hyperplasia (n = 58), Complex hyperplasia (n = 22), Atypical hyperplasia (n = 9), Adenocarcinoma (n = 16). Immunohistochemistry method was used through estreptavidin biotin peroxidase diaminobenzidine. The samples were analyzed for computer image. Mendez modified technique for cell count was used. The statistical analysis included descriptive statistical. The differences between groups were analyzed using ANOVA-MANOVA and t of Student for independent samples (alpha = 0.05) and other statistical test. RESULTS: there were no statistical significant differences on the cell density with ER between the normal endometrial and the simple and complex hyperplasia. The estrogen receptors are decreased in the atypical hyperplasia and the endometrial adenocarcinoma. DISCUSSION: The gravity of the injury increases proportionally with the age; the most important factor in the reduction of the cells with estrogénicos receivers is the neoplastic transformation.


Assuntos
Adenocarcinoma/química , Neoplasias do Endométrio/química , Receptores de Estrogênio/análise , Hiperplasia Endometrial , Feminino , Humanos , Imuno-Histoquímica , Pessoa de Meia-Idade , Estudos Retrospectivos
15.
BMC Infect Dis ; 6: 27, 2006 Feb 20.
Artigo em Inglês | MEDLINE | ID: mdl-16504014

RESUMO

BACKGROUND: HPV infection in women from developing countries is an important public health problem. Therefore, we sought to determine the prevalences of HPV infection and HPV genotypes in a female population of Durango City, Mexico. Also to determine whether any socio-demographic characteristic from the women associated with HPV infection exists. METHODS: Four hundred and ninety eight women seeking cervical Papanicolaou examination in three public Health Centers were examined for HPV infection. All women were tested for HPV DNA PCR by using HPV universal primers. In addition, all positive HPV DNA PCR samples were further analyzed for genotyping of HPV genotype 16, 18 and 33. Socio-demographic characteristics from each participant were also obtained. RESULTS: Twenty-four out of four hundred and ninety-eight (4.8%) women were found infected by HPV. HPV genotype 16 was found in 18 out of the 24 (75%) infected women. Two of them were also coinfected by HPV genotype 18 (8.3%). In the rest 6 PCR positive women, genotyping for HPV genotypes 16, 18 and 33 were negative. CONCLUSION: The prevalence of HPV in women of Durango City is low; however, most infected women have high risk HPV genotype. The women who were studied showed low frequency of risk factors for HPV infection and this may explain the low prevalence of HPV infection. The high frequency of high risk HPV genotypes observed might explain the high rate of mortality for cervical cancer in our region.


Assuntos
Teste de Papanicolaou , Papillomaviridae/genética , Papillomaviridae/isolamento & purificação , Infecções por Papillomavirus/epidemiologia , Infecções por Papillomavirus/virologia , Esfregaço Vaginal , Adulto , Idoso , Feminino , Genótipo , Humanos , México/epidemiologia , Pessoa de Meia-Idade , Prevalência
16.
Ginecol. obstet. Méx ; 65(6): 235-8, jun. 1997. tab
Artigo em Espanhol | LILACS | ID: lil-210772

RESUMO

El presente estudio se realizó con el propósito de establecer la sensibilidad y especificidad del Papanicolaou y para valorar si la colposcopía de rutina mejora los resultados obtenidos con el Papanicolaou. Para ello se seleccionaron aleatoriamente 150 pacientes que acudieron para citología de rutina al Instituto de Investigación Científica de la U.J.E.D. Todas fueron sometidas a colposcopía y biopsia cervical. Con el objetivo de no sesgar los resultados, el colposcopista no conocía el diagnóstico citológico y el patólogo desconocía el reporte de ambos estudios. El significado estadístico se determinó mediante la chi cuadrada (diferencia significativa de P<0.05). Veintitrés pacientes (15.3 por ciento) tenían patología cervical de acuerdo al resultado de la biopsia. El Papanicolaou sólo detectó 4/23 (17 por ciento) de las mujeres con enfermedad del cérvix mientras que la colposcopía reconoció 20/23 (87 por ciento) de las pacientes, (P<0.001). Quince de 27 pacientes (55.5 por ciento) con Papanicolaou negativo tenían colposcopía anormal y biopsia compatible con neoplasia intraepitelial cervical (NIC). Se concluye que es necesario incrementar la sensibilidad y que la colposcopía anormal y biopsia compatible con neoplasia intraepitelial cervical (NIC). Se concluye que es necesario incrementar la sensibilidad y que la colposcopía puede mejorar los resultados obtenidos con la citología al detectar patología cervical no diagnosticada por el Papanicolaou


Assuntos
Adulto , Pessoa de Meia-Idade , Humanos , Feminino , Displasia do Colo do Útero/diagnóstico , Colposcopia , Diagnóstico Diferencial , Doenças do Colo do Útero/diagnóstico , Neoplasias do Colo do Útero/diagnóstico , Esfregaço Vaginal
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