RESUMO
Johne's disease is chronic granulomatous infectious enteritis of animals caused by Mycobacterium avium subspecies paratuberculosis. A total of 153 animals from 19 dairy farms, 2 gaushalas (unproductive-animal rehabilitation centers), 2 goat and 2 sheep farms from different districts of the Punjab region were selected on the basis of clinical signs of disease. All samples from cattle (n=86), buffalo (n=34), goat (n=25) and sheep (n=26) were subjected to Ziehl-Neelsen staining and DNA extraction by a freeze and thaw method. Ziehl-Neelsen staining detected 71% samples positive for acid-fast bacilli whereas IS900 PCR detected 55% positive for Map DNA. IS1311 PCR-REA analysis of IS900 positive samples revealed 'Bison' type as the most prevalent (82%) genotype of Map, infecting all domestic ruminants. 'Cattle' type was present in a minority of cases (15%) from cattle, buffaloes and goats. This is the first report of 'Cattle' type Map from buffalo and goat species in India.
Assuntos
Mycobacterium avium subsp. paratuberculosis/genética , Paratuberculose/diagnóstico , Paratuberculose/epidemiologia , Reação em Cadeia da Polimerase , Polimorfismo Genético , Ruminantes/microbiologia , Animais , Técnicas de Tipagem Bacteriana , Bovinos , Índia/epidemiologia , Epidemiologia Molecular , Paratuberculose/microbiologia , Mapeamento por RestriçãoRESUMO
The superiority of flexible to rigid sigmoidoscopy is resulting in its increasing utilization by primary care physicians. The procedure is relatively risk-free, but complications including perforations may occur. An unusual case of cecal perforation as a result of flexible sigmoidoscopy is presented in order to draw attention to this complication.
Assuntos
Ceco/lesões , Perfuração Intestinal/etiologia , Sigmoidoscopia/efeitos adversos , Tecnologia de Fibra Óptica/instrumentação , Humanos , Perfuração Intestinal/patologia , Masculino , Pessoa de Meia-Idade , SigmoidoscópiosRESUMO
An unusual patient with blue rubber bleb nevus syndrome is presented. At birth a single abdominal wall hemangioma was noted, and over a 21-year follow-up the full syndrome developed, including many cutaneous and intestinal lesions and anemia. Our long-term follow-up of this patient allows an understanding of the natural history of this condition, especially regarding recurrence of lesions after excision.