[Biodistribution of the recombinant heat shock protein rhHsp70 in intracranial C6 glioma models in Wistar rats and subcutaneos B16/F10 melanoma in C57BL/6 mice].

For the first time, the biodistribution of recombinant heat shock protein in rhHsp70 rats with grafted intracranial C6 glioma was evaluated. It was assessed using the fluorescent antibody accumulation chaperone rhHsp70 conjugated with fluorochrome Alexa Fluor 555 in tumor cells by intratumoral or intravenous administration. Assessment of the distribution and accumulation of labeled protein was carried out on the model of subcutaneous B16/F10 melanoma in C57BL/6 mice with the use of single-photon emission computer tomography. After 60 minutes after intravenous administration rhHsp70-I123 (20 MBq, 5 mg chaperone) accumulation of the drug mainly in the liver and tumor tissue was showed. The coefficient of the differential accumulation of the labeled protein KDN(tumor/background) was 3.14. It was turned out that comparing the level of fixation of rhHsp70-I123 in the liver and the tumor KDN(tumor/ liver) = 0.76. After 24 hours from the time of injection of rhHsp70-I123 it was observed increase the level of fixation of the labeled protein in the liver and melanoma: KDN(tumor/background) = 3.43; KDN(tumor/liver = 0.78.

[Role of 18F-FDG PET in tumor grading and evaluating effectiveness of therapy in malignant lymphomas].

The report compares the results of (multi-layer spiral computed tomography) (MSCT), 67 Ga citrate lymph scintigraphy and 18FDG PET in patients with Hodgkin's disease. The predictive significance of the latter method exceeded that of MSCT and ultrasound in diagnosing lymph node neoplasia below the diaphragm. As far as peripheral and mediastinal lymph nodes are concerned, the MSCT efficiency of the three procedures was comparable. Advantage was offered by PET in early evaluation of therapeutic effectiveness. Moreover, its practical significance for assessing tumor sensitivity to therapy as well as differentiation between viable tumor tissue and fibrosis was demonstrated.

[Role of 123-I-metaiodobenzylguanidine imaging as a component in the diagnosis and treatment of neuroblastoma].

Tomoscintigraphy with 123I-metaiodobenzylguanidine reveals function of tumor tissue. It may be used for both primary diagnosis of neuroblastoma, monitoring treatment efficiency and prognosis. Before single photon emission tomography using 123I-metaiodobenzylguanidine is undertaken, MRI by means of computed tomography should be carried out in abdominal area to ensure perfect blending of multimodal images.

[Clinical experience in using 18F-FDG PET in the staging and restaging of malignant lymphomas].

The present study was undertaken to determine the diagnostic accuracy of positron emission tomography (PET) in the staging and restaging of malignant lymphomas. For determination of the extent of a neoplastic process, complex radiation examination was conducted in 67 patients with malignant lymphomas. In 12 (17.9%) cases, the stage of the disease was changed, as evidenced by PET. There is evidence that the technique is of high diagnostic accuracy in detecting malignancies of l ymph nodes, skeletal system, and parenchymatous organs in lymphoproliferative diseases.

[Changes in secretion of sex steroid hormones during stress in rats with various brain excitability]. / Izmenenie sekretsii polovykh steroidnykh gormonov pri stresse u krys s raznoi vozbudimost'iu mozga.

A stress procedure decreased the plasma progesterone faster in highly excitable rats than in the less excitable those. The decrease in gonadal and adrenal progesterone was still obvious in 24 hours in both groups of rats but more obvious in the former group in respect to the progesterone level. The data obtained may help to understand individual ability to respond to a stress in a different way depending on functional condition of the hypothalamo-pituitary-adrenal axis.

[The effect of local chemical sympathectomy of the rat uterus on its sensitivity to sex hormones]. / Vliianie mestnoi khimicheskoi desimpatizatsii matki krys na ee chuvstvitel'nost' k polovym gormonam.

Chemical sympathectomy of the rat uterus did not alter the main metabolic processes maintaining the uterus weight even though it changed its response to sex steroids. Sensitivity of endo- and myometrium to progesterone significantly decreased thus inducing a relative increase in the estradiol action.

[The effect of postnatal sympathectomy on the production and receptor binding of sex steroids in female rats during puberty]. / Vliianie postnatal'noi desimpatizatsii na produktsiiu i retseptsiiu polovykh steroidov u samok krys v period pubertata.

The total chemical desympathization caused by injection of 6-hydroxydopamine in the first day of life increased the progesterone secretion, decreased the estradiol secretion and abolished translocation of estradiol receptors to the cell nuclei in the uterus of 1-2 days old rats, which correlated with the absence of uterus weight increase after exogenous estradiol injections. The peripheral desympathization caused by injection of neurotoxin to two weeks old rats induced only estradiol secretion decrease, but to the less extent than after total desympathization.

[The content of sex steroid receptors and the activity of marker enzymes of estrogenic action in the uterus of androgen-sterile rats]. / Soderzhanie retseptorov polovykh steroidov i aktivnost' nekotorykh fermentov-markerov éstrogennogo deistviia v matke u androgensteril'nykh krys.

In rats with preserved ovaries, both in normal and in neonatally androgenized, the activity of enzymes kreatinphosphokinase and peroxidase cannot serve as the marker of the estradiol effect upon the uterus. In ovariectomised animals, the dynamics of activity of these enzymes corresponds to uterotropic responses to estradiol. but not to the dynamics of hormone receptor complexes. The data obtained makes doubtful the participation of the hormone receptor complexes in realisation of the estrogens effect upon the uterus.

[Feto-placental system in diabetes mellitus and hydramnios]. / Feto-platsentarnaia sistema pri sakharnom diabete i mnogovodii.

The fetoplacental system of 132 women with diabetes mellitus and 85 healthy women was studied during the third pregnancy trimester. Blood estradiol and urinary estriol levels were found-reduced in the diabetics, particularly in those with insulin-dependent diabetes. Blood progesterone and oxytocinase levels were found unchanged. These parameters lowered in diabetic glomerulosclerosis. If pregnant diabetics develop hydramnion, their blood estradiol, progesterone, prolactin, and oxytocinase levels increased.

[The effect of aminostimil on the regulation of sexual function in rats]. / Vliianie aminostimila na reguliatsiiu polovoi funktsii u krys.

The effect of aminostimil: a synthetic drug of the acylhydrazide group, was studied in white rats. Aminostimil decreased the uterus weight but did not interfere with estrogen positive action in ovariectomized (OVX) rats, although it increased the FSH level, abolished the inhibitory effect of estradiol on the FSH secretion and did not change the secretion of the LH. Aminostimil induced accumulation of GnRH in the preoptic area and median eminence area in intact female and male rats, LH concentration in the blood plasma but decreased. The data obtained suggest that the antiestrogenic effect of aminostimil is due to inhibition of the GnRH secretion from the hypothalamus into the portal blood.

[Hormonal regulation of reproductive function in rats in response to cold stress in a context of chronic toluene poisoning]. / Gormonal'naia reguliatsiia reproduktivnoi funktsii u krys pri kholodovom stresse na fone khronicheskoi intoksikatsii toluolom.

Chronic toluene inhalation at a maximum permissible concentration (MPC) and 10-fold higher as well as cold stress result in a significant rise of blood prolactin in female rats. Cold stress in control animals causes a decrease in the level of gonad-releasing hormone (Gn-RH) in the hypothalamus. When exposed to toluene this reaction disappears. In 4 hours after estradiol administration control animals demonstrate an increase in the level of Gn-RH in the vascular organ of an end plate. Rats, exposed to toluene (in MPC concentration) and cold stress show a decrease in the level of hypothalamic Gn-RH. At a higher concentration of toluene the level of Gn-RH in the hypothalamus remains unchanged either after estradiol administration or in cold stress. Thus chronic toluene inhalation even at small concentrations changes hypothalamic reactivity in response to stress and hormonal stimuli.

[The negative and positive phases of the reaction of the hypothalamo-hypophyseal-gonadal system in female rats to the administration of sex hormones]. / Otritsatel'naia i polozhitel'naia fazy reaktsii gipotalamo-gipofizarno-gonadnoi sistemy samok krys na vvedenie polovykh gormonov.

I.m. administration of estradiol benzoat in physiological doses decreased 3-fold the lutropin secretion and follitropin secretion by one third, in ovariectomised rats. The same dose in intact rats decreases the lutropin level in the blood and gonadoliberin contents in the medial eminence by the negative feedback mechanism. In 4-6 hrs after injection the positive effect of estradiol is at its highest level, the lutropin level in the blood increases by 80-100 times, the follitropin--by 2-3 times. Therefore, estradiol plays a decisive role in positive as well as negative effects on the lutropin secretion, whereas its effect upon follitropin secretion is less obvious. The gonadoliberin transport in negative phase of estradiol effect is mainly performed through the medial eminence whereas the positive effect of estradiol needs a participation of organum vasculosum of the lamina terminalis too.

[17 beta hydroxysteroid dehydrogenase and creatine phosphokinase activities and the levels of sex hormones and their receptors in the uterus of patients with fibromyoma or endocrine sterility]. / Aktivnost' 17beta-gidroksisteroiddegidrogenazy, kreatinfosfokinazy, soderzhanie polovykh steroidov i ikh retseptorov v matke u bol'nykh s fibromiomoi ili éndokrinnym besplodiem.

Examination of endometrial and myometrial activity of steroidogenic enzyme markers, 17 beta-hydroxy steroid dehydrogenase (17 beta-HOSD) and creatine phosphokinase (CP), in women with fibromyoma or endocrine infertility has demonstrated that endometrial 17 beta-HOSD activity corresponded to ovarian progesterone secretion rates but did not correlate with cytosolic progesterone secretion rates but did not correlate with cytosolic progesterone receptor levels. The magnitude of uterine effects of endogenous progesterone in the patients with endocrine infertility and fibromyoma may be inferred from (1) hormone ability to induce 17 beta-HOSD, and (2) ability to reduce cytosolic progesterone receptor levels. Neither endometrial nor myometrial CP represents a "pure" marker of estrogenic influence.

[The dependence of estradiol and progesterone reception in the endometrium on its histological structure in women with the syndrome of luteal phase failure]. / Zavisimost' retseptsii éstradiola i progesterona v éndometrii ot ego gistostruktury u zhenshchin s sindromom nedostatochnosti liuteinovoi fazy tsikla.

A study of the level of the sex steroid receptors in the endometrium of women with the syndrome of insufficiency of the luteal phase of the cycle (ILP-syndrome) has shown that disorders of receptor processes affect, first of all, progesterone receptors. An increase in a degree of a delay of endometrial development was accompanied by a rise of the level of progesterone in the endometrium which depended on the estradiol-progesterone ratio in the circulation, detectable on the 20th day of the menstrual cycle. The ILP-syndrome is characterized by the sufficient induction of progesterone receptors at the end of the proliferative--the beginning of the luteal phase of the cycle and by their insufficient inhibition with a low level of progesterone in the middle of the luteal phase. Insufficient synthesis of receptors as a result of sharp hypoestrogenemia is less frequent. The primary affection of the receptor apparatus as a cause of the ILP-syndrome against a normal hormonal background in the examinees was undetectable.

[The synthesis of estradiol and progesterone receptors in relation to the biological effects of sex hormones in ovariectomized rats]. / Sintez retseptorov éstradiola i progesterona v sviazi s biologicheskimi éffektami polovykh gormonov u ovariéktomirovannykh krys.

The concentration of the estradiol and progesterone receptors in cytosol increased within a week after ovariectomy in rats, the nucleic concentration of receptors sharply diminishing. The uterus atrophy following ovariectomy in associated with a reduction of both nucleic and cytosol receptors in the organ. Administration of estradiol (1 mg) restored the organ mass and the character of hormonal reception within a week. Progesterone and clomyphen exerted some uterotropic effect. Ovariectomy reduced the creatinkinase activity by 2.9 times and the peroxydase activity by 13 times. Two types of synthesis of female sex hormones receptors seem to exist in the rat uterus: the constitutional type depending on no hormonal effects and the induced one depending on the sex steroids, estradiol in particular.

[Endometrial estradiol and progesterone receptors in women with various clinical forms of luteal insufficiency]. / Retseptsiia éstradiola i progesterona v éndometrii u zhenschin s razlichnymi klinicheskimi formami liuteinovoi nedostatochnosti.

Changes in uterine estradiol and progesterone receptor content in patients with luteal insufficiency (the insufficient luteal phase/ILP/ syndrome developing in the presence of tubal and uterine inflammations, hyperandrogenism or insignificantly increased prolactin level were described. The level of steroid receptors is correlated to blood levels of the respective hormones throughout the cycle. It is suggested that changes in sex steroid receptors are secondary to disorders of progesterone and estradiol secretion in cases of the ILP syndrome developing in the presence of uterine and tubal inflammations, and in patients with increased blood prolactin. In hyperandrogenic patients with the ILP syndrome, nuclear progesterone receptors night possibly be inadequate.

[Sexual dimorphism in the content of luliberin following deafferentation of the mediobasal hypothalamus]. / Polovoi dimorfizm v soderzhanii liuliberina posle deafferentatsii mediobazal'nogo gipotalamusa.

Luliberin (LH-RH) was assayed radioimmmunologically in the hypothalamic fragments taken from male and female rats, intact or with isolated mediobasal hypothalamus. An increased amount of LH-RH was found in the fragments containing organum vasculosum of the lamina terminals (OVLT) and taken from intact males. LH-RH amount was negligible in isolated parts of hypothalamic fragments containing arquate nucleus of median eminence. OVLT of females was mu;ch richer with LH-RH than that of the males. The females though were characterized by a lower blood lutropin. The inhibitory influences on the secretion of LH-RH seem to be more obvious in intact males than in females.

[Reception of progesterone and its biological action in the uterus of neonatally androgenized rats]. / Retseptsiia progesterona i ego biologicheskoe deistve v matke neonatal'no androgenizirovannykh krys.

The relationship of the level of uterine progesterone receptors and plasma concentration of female sex steroids with the biological effect of progesterone (its effect on collagen content and the number of uterine endometrial glands) was studied in neonatally androgenized female rats aged 90-120 days. In these animals the level of progesterone receptors in the uterine cytosol fraction was raised, in the nuclear fraction it was lowered. Plasma concentration values of estradiol and progesterone in such animals did not differ from those of intact female rats. The ability of estradiol to induce the synthesis of uterine progesterone receptors was less noticeable in androgen sterile animals. The sensitivity of the uterus to progesterone in such animals was lowered either. Progesterone doses causing a significant decrease in the concentration of progesterone receptors in cytosol and in uterine tissue collagen content, proved to be ineffective for androgen sterile female rats. Disturbance of progesterone reception and a related decrease in the sensitivity of the uterus of androgen sterile animals to this hormone can be probably determined by an irreversible androgen effect on the target cell genome in the early neonatal period, not depending on the peculiarities of hormonal regulation in adult rats.

[Interrelation of the hormonal indices and the cytologic characteristics of the germ cells]. / Vzaimosviaz' mezhdu gormonal'nymi pokazateliami i tsitologicheskoi kharakteristikoi polovykh kletok.

The analysis of the maturation of follicular oocytes as well as of the state of gametes after induction of ovulation with PMSG or with GnRH in intact and androgenized rats revealed the dependence of the heterogeneity extent (in morphologic and chromosomal damages) of population of germ cells maturing in vivo and in vitro upon the character of gonadotropin regulation disturbances of folliculogenesis. Stable disorder of reproductive function (androgenization) is followed by a significant increase in number of abnormal gametes under its hormonal correction.