RESUMO
The uterine endometrial surface of bovines is in constant exposureconstantly exposed with to a multitude ofmany microbial populations that changes throughout the post-partum phase in terms of complexity and dynamics. These microbes contribute to the host pathology, leading to severe economic losses along withnd reproductive capabilities. The basic primary interface that occurs between the internal tissues of the body of the hostbetween the host body's internal tissues and the microbes is the endometrial surface of the uterus. As a result of the infinite pathogenic population, there is always a danger for the opportunistic organisms to attack. Therefore, it is paramount that any interactions, especially microbial microbes with the endometrial surface, are regulated by the host cells. However, the inflammatory response as the defense mechanism contributes a pivotal roleis pivotal in host immunity and pathology. The inflammatory cascade and pathways are important essential to eliminate this clinical problem. In this review, we will discuss and explain how the inflammation and the various components of the immune system play their role in host pathology and therapeutic strategies, taking into account the interface between the host and the microbes on the surface of the endometrium. This review is also instrumental in further explanation of inflammatory uterine disease by discussing the response of inflammation to external insult.
Assuntos
Endometrite , Feminino , Animais , Bovinos , Humanos , Endometrite/tratamento farmacológico , Endometrite/veterinária , Inflamação/patologia , Útero/patologia , Endométrio , ReproduçãoRESUMO
This present study was designed to develop a novel microbiological inhibition-based method for the rapid screening and identification of antibiotic residues in milk, chicken egg and honey. Geobacillus stearothermophilus C953 was used as test bacterium in the detection system of this study. The optimization of nutrients and other supplements were performed to promote the growth of test bacterium and thus shorten the detection time. Furthermore, the synergetic agents were added to improve the sensitivity of test bacterium to more antibiotics. Additionally, confirmatory solutions such as ß-lactamase, p-aminobenzoic acid, MgSO4 and cysteine were added to classify and identify different kinds of antibiotics. We observed that the LOD of this detection system was at or close to maximum residue limits established by EU for ß-lactams, aminoglycosides, tetracyclines, sulfonamides, macrolides and quinolones in milk. The LOD of different kinds of antibiotics in chicken egg was less than or similar to the MRL and the LOD of Premi®test (except sulfonamides). For honey, there are no MRL, the LOD was less than or similar to the recommended concentration and the LOD of Premi®test. Noteworthy, the detection system also can identify these six kinds of antibiotics in milk, chicken egg and honey, and there were satisfactory results of specificity experiments and confirmation experiments by LC-MS/MS. Accordingly, the present study provides a reliable preliminary characterization of antibiotic residues in animal foods and improves the detection efficiency for the following chemical confirmation experiments by HPLC, LC-MS/MS, immunological and receptor-based tests.
Assuntos
Resíduos de Drogas , Mel , Animais , Antibacterianos/análise , Galinhas , Cromatografia Líquida , Resíduos de Drogas/análise , Contaminação de Alimentos/análise , Mel/análise , Leite/química , Espectrometria de Massas em TandemRESUMO
Newcastle disease (ND), caused by virulent Avian avulavirus 1 (AAvV 1), affects variety of avian species around the globe. Several AAvV 1 viruses of different genotypes have recently emerged with varying clinical impacts on their susceptible hosts. Although experimental infection with velogenic and mesogenic strains in chickens and pigeons is well-studied, nevertheless, there exists a paucity of data for comparative variations in serum biochemistry profile of susceptible hosts upon challenge with isolates of varying pathogenicities. With this background, a comparative assessment of a range of serum biochemical parameters was made following challenge with duck-originated velogenic strain (sub-genotype VIIi; MF437287) and pigeon-originated mesogenic strain (sub-genotype VIm; KU885949) in chickens and pigeons. For each of the isolate, commercial broiler chickens and wild pigeons were challenged (10-6.51 EID50/0.1 mL for sub-genotype VIIi and 10-6.87 EID50/0.1 mL sub-genotype Vim) separately via intranasal and intraocular route. Sera were collected on 0, 3rd, 5th, 7th, and 9th day post-infection (dpi), and processed for quantitative analysis of different biochemical parameters. By day 3 post-infection (pi), a substantial decrease (p < 0.0001) in serum alkaline phosphatase (ALP) was observed in chickens and pigeons challenged with velogenic isolate. On the other hand, from day 5 pi and onward, a significant increase (p < 0.001) in serum ALP and total protein concentration was observed exclusively in pigeons challenged with mesogenic isolate. For serum aspartate aminotransferase (AST), a significant increase (p < 0.05) in concentration was observed on day 3 pi which decreased from day 5 pi and onward in pigeons and chickens challenged with mesogenic isolate. Also, to reveal antigenic differences among homologous and heterologous vaccine and field-prevalent strains, cross-hemagglutination inhibition assay demonstrated antigenically diverse nature (R-value < 0.5) of both strains from vaccine strain (LaSota, genotype II). The study concludes antigenic differences among prevalent genotypes than vaccine strain and, although requires further studies to ascertain study outcomes, the serum biochemical profile may facilitate presumptive diagnosis of disease in their susceptible hosts.
Assuntos
Doenças das Aves/virologia , Galinhas , Columbidae , Doença de Newcastle/virologia , Vírus da Doença de Newcastle/fisiologia , Animais , Doenças das Aves/sangue , Doenças das Aves/imunologia , Análise Química do Sangue/veterinária , Testes de Inibição da Hemaglutinação/veterinária , Doença de Newcastle/sangue , Vírus da Doença de Newcastle/genética , Doenças das Aves Domésticas/sangue , Doenças das Aves Domésticas/imunologia , Doenças das Aves Domésticas/virologiaRESUMO
BACKGROUND: Newcastle disease (ND) is one of the most deadly diseases of poultry around the globe. The disease is endemic in Pakistan and recurrent outbreaks are being reported regularly in wild captive, rural and commercial poultry flocks. Though, efforts have been made to characterize the causative agent in some of parts of the country, the genetic nature of strains circulating throughout Pakistan is currently lacking. MATERIAL AND METHODS: To ascertain the genetics of NDV, 452 blood samples were collected from 113 flocks, originating from all the provinces of Pakistan, showing high mortality (30-80%). The samples represented domesticated poultry (broiler, layer and rural) as well as wild captive birds (pigeons, turkeys, pheasants and peacock). Samples were screened with real-time PCR for both matrix and fusion genes (1792 bp), positive samples were subjected to amplification of full fusion gene and subsequent sequencing and phylogenetic analysis. RESULTS: The deduced amino acid sequence of the fusion protein cleavage site indicated the presence of motif (112RK/RQRR↓F117) typical for velogenic strains of NDV. Phylogenetic analysis of hypervariable region of the fusion gene indicated that all the isolates belong to lineage 5 of NDV except isolates collected from Khyber Pakhtunkhwa (KPK) province. A higher resolution of the phylogenetic analysis of lineage 5 showed the distribution of Pakistani NDV strains to 5b. However, the isolates from KPK belonged to lineage 4c; the first report of such lineage from this province. CONCLUSIONS: Taken together, data indicated the prevalence of multiple lineages of NDV in different poultry population including wild captive birds. Such understanding is crucial to underpin the nature of circulating strains of NDV, their potential for interspecies transmission and disease diagnosis and control strategies.