PBAT/PLA food packaging film containing sodium dehydroacetate-loaded diatomite as an antibacterial agent: Fabrication, water-gas regulation and long-acting antimicrobial mechanism.

Biodegradable food packaging films with good antimicrobial properties are highly sought after for prolonging the shelf-life of fruits and vegetables whilst minimizing waste streams originating from the food sector. In this work, a series of PBAT/PLA food packaging films containing sodium dehydroacetate-loaded diatomite (SD/D) as an antimicrobial agent were fabricated. Structural analyses showed that the sodium dehydroacetate was incorporated into the pores of the diatomite. A uniform dispersion of SD/D in the composite films effectively enhanced water and gas permeability, whilst also giving the films good mechanical properties. The slow release of SD endowed the composite films with long-acting antibacterial ability (>90 % bacteriostasis rate for E. coli and >85 % bacteriostasis rate for S. aureus). The composite films were able to effectively maintain the quality of banana fruits during storage at room temperature, encouraging their use in food applications where non-biodegradable petrochemical-derived packaging films have traditionally been used.

Surface Plasmon Resonance Spectroscopy for Detection of S-Nitrosylated Proteins.

Protein S-nitrosylation, the NO-dependent regulatory mechanism, is a posttranslational modification of reactive cysteine thiols to form S-nitrosothiols. The biotin-switch technique (BST) has become a mainstay method for detection of S-nitrosylated proteins in biological samples. On the basis of BST, we describe a surface plasmon resonance (SPR) spectroscopy method for detecting S-nitrosylated proteins. This method can be applied for the indirect determination of S-nitrosylated proteins in biological samples.