Exploring copper (II) porphyrin complexes and their derivatives for electrochemical analysis and biological assessment in the study of breast cancer (MCF-7) cell lines.

In this study, several derivatives of tetraphenylporphyrin were synthesized, each with unique meso-substituent groups including phenyl, methoxyphenyl, butyloxyphenyl, octyloxyphenyl, and dectyloxyphenyl. Additionally, their corresponding copper complexes were prepared and thoroughly characterized. The structural confirmation of all compounds was established through CHN elemental analysis, mass spectrometry, and FT-IR spectroscopy. As the number of carbon atoms in the alkyl long-chain increased, a slight red shift in the electronic absorption band was observed, which was attributed to the electronic influence of the alkyl group. DFT analysis indicated that electron density predominantly localized on the porphyrin ring of both the metal free porphyrins and copper (II) porphyrin complexes, with relatively low electron density in the p orbital of the meso-aryl long-chain substituent group. EPR spectroscopy of the Copper (II) ion complexes revealed signals, indicating their paramagnetic properties. Additionally, the Copper (II) tetraphenylporphyrin (CuTPP) complexes displayed two reversible oxidation peaks at +0.97 V and +1.35 V, whereas other derivatives exhibited lower oxidation potentials. The cytotoxicity of these compounds against MCF-7 cell lines was assessed using MTT assay, revealing cytotoxic effects in all cases. Among them, Copper (II) tetrakis (4-methyloxyphenyl)porphyrin (CuTOMPP) demonstrated the highest potential, with an IC50 value of 32.07 µg/mL.

Synthesis and biocompatible role of hierarchical structured carbon nanoplates incorporated α-Fe2O3 nanocomposites for biomedical applications with respect to cancer treatment.

This study aimed to inspect the hierarchically structured spherical-like hematite (α-Fe2O3) nanoparticles synthesize by simple, low temperature solution combustion process. The uniformly distributed α-Fe2O3/carbon nanocomposite (α-Fe2O3/C nanocomposite) was prepared by incorporating carbon nanoplates into sphere-like α-Fe2O3 nanoparticles. The synthesized nanomaterials were characterized using various techniques such as XRD, FESEM, and EDS. The cytotoxicity of the material was evaluated by MTT assay and nuclear imaging based on the cell morphological changes on both human lung cancerous cell line A549 and chang liver as non cancerous cell line. The results demonstrated that the pure and composite material exhibited above 70% viability on non-cancerous cell line and around 60% inhibition on A549 lung cancer cell line indicates the α-Fe2O3/C nanocomposite is biocompatible and can be used for biological applications and anticancer therapy. Cell death induced by α-Fe2O3, carbon nanoplates and α-Fe2O3/C nanocomposites was further evidenced with DAPI.