Polyphenolic glycosides with unusual four-membered ring possessing anti-Parkinson's disease potential from black wolfberry.

This work reports the isolation of seven undescribed polyphenolic glycosides (1-7) together with fourteen known compounds (8-21) from the fruit of Lycium ruthenicum Murray. The structures of the undescribed compounds were identified based on comprehensive spectroscopic methods including IR, HRESIMS, NMR and ECD, and chemical hydrolysis. Compounds 1-3 possess an unusual four-membered ring, while 11-15 were firstly isolated from this fruit. Interestingly, compounds 1-3 inhibited monoamine oxidase B with IC50 of 25.36 ± 0.44, 35.36 ± 0.54, and 25.12 ± 1.59 µM, respectively, and showed significant neuroprotective effect on PC12 cells injured by 6-OHDA. Moreover, compound 1 improved the lifespan, dopamine level, climbing behavior, and olfactory ability of the PINK1B9 flies, a Drosophila model of Parkinson's disease. This work presents the first in vivo neuroprotective evidence of the small molecular compounds in L. ruthenicum Murray fruit, indicating its good potential as neuroprotectant.

Association of cerebrospinal fluid advanced oxidation protein products levels with early brain injury and prognosis of aneurysmal subarachnoid hemorrhage: A preliminary prospective study.

OBJECTIVES: In this study, we investigated the time course in the cerebrospinal fluid (CSF) advanced oxidation protein products (AOPPs) levels in patients with aneurysmal subarachnoid hemorrhage (aSAH), and ascertained the relationship between the levels of AOPPs and early brain injury (EBI), hydrocephalus and prognosis of patients with aSAH. METHODS: We measured the CSF AOPPs levels in 50 patients with aSAH at 1-3 d, 4-6 d, 7-9 d, and 10-12 d after hemorrhage. The modified Fisher grades, Hunt-Hess grades, CSF IL-6 levels, peripheral blood count of white blood cells, cerebral edema scores and hydrocephalus were used to assess the severity of brain injury. Modified Rankin Scale (mRS) scores were used to assess the prognosis. Patients with mRS scores greater than 2 were considered to have a poor outcome. RESULTS: CSF AOPPs levels were significantly higher in patients with aSAH with poor prognosis, compared to patients with good prognosis and peaked in the early stage. Among patients with aSAH, the levels of CSF AOPPs on days 1-3 were significantly correlated with modified Fisher grades, Hunt-Hess grades, CSF IL-6 levels, peripheral blood count of white blood cells, and cerebral edema scores. Also, in patients with hydrocephalus, early CSF AOPPs levels were significantly elevated. Levels of CSF AOPPs in aSAH patients on days 1-3, 4-6, and 7-9 were independently associated with poor prognosis at the 90-day follow-up, and the optimal area under the curve (AUC) values for CSF AOPPs levels were found on days 1-3. CONCLUSIONS: AOPPs may serve as the potential biomarker to assess the severity of EBI and prognosis in patients with aSAH.

Modification-bioremediation of copper, lead, and cadmium-contaminated soil by combined ryegrass (Lolium multiflorum Lam.) and Pseudomonas aeruginosa treatment.

The principal objective of this study was to investigate the strengthened remediation effect and relevant mechanism of P. aeruginosa on ryegrass (Lolium multiflorum Lam.) for soil contaminated by Cu-Pb-Cd compound heavy metals. The results showed that the complex heavy metals' contamination had remarkable inhibiting effect on the growth of plants (P < 0.01), and the biomass of ryegrass's stem and leaves declined by 28.2%, while that of roots decreased by 34.7% after 45 days. The inoculation of P. aeruginosa promoted the growth of ryegrass in polluted soil, in which the biomass recovered to the same level of that in normal plant; the activity of both catalase and urease in the soil also increased strikingly (by 29.3% and 75.7%, respectively); the ratio of residual heavy metals in the soil decreased, while the acid extractable heavy metals increased notably. Therefore, the absorption and accumulation of ryegrass to the heavy metals in soil were improved to some extent; the bioconcentration factor of Cu, Pb, and Cd in ryegrass increased by 35.9%, 55.6%, and 283.5%, respectively. The exterior microorganism allowed the accumulation of Cu, Pb, and Cd in shoots of ryegrass increasing remarkably, while in roots, only the accumulation of Pb increased by 16.3%, and that of both Cu and Cd decreased. Besides, in the P. aeruginosa-inoculated system, the transfer factor of Cu and Cd in plants increased strikingly, while that of Pb decreased.

[Physiological Responses of Ryegrass in Cadmium-Nonylphenol Co-contaminated Water and the Phytoremediation Effects].

Physiological responses of ryegrass in cadmium-nonylphenol (Cd-NP) co-contaminated water, as well as the phytoremediation effect were studied under laboratory conditions. The experiment revealed that the biomass and chlorophyll content of ryegrass significantly declined at high concentrations of Cd2+(10 mg·L-1), while POD and PPO activities significantly increased. No significant changes to the biomass, chlorophyll content and MDA of ryegrass were observed in the presence of NP. POD activities significantly increased at high concentrations of NP (5 mg·L-1). The addition of high NP concentrate reduced the inhibition of Cd under co-contaminated stress, the MDA content and PPO activities both reduced. The experiment also revealed that the removal rate of Cd2+ by ryegrass reached 55.3% after 12 h. The absorption efficiency was improved by high NP concentration. Absorption efficiency of Cd2+ by the subterranean part of the plant significantly increased in presence of NP, and the absorption efficiency increased with the increase of NP concentration. The absorption rate of NP was 44.6% after 24 h at NP concentration of 5 mg·L-1. The removal rate was slightly influenced at low concentrations of Cd2+, but significant inhibition of absorption and degradation was observed at high concentrations of Cd2+.

[Effect of Cadmium on Biodegradation of Nonylphenol by Pseudomonas aeruginosa].

The influence of Cd on the degradation of nonylphenol (NP) by P.aeruginosa SH1 was investigated in this study.The investigation revealed that biomass of the strain was significantly declined with the increase of Cd2+ concentration.The biomass was declined by 27.1% in the presence of 10 mg·L-1Cd2+ after 24 h.The addition of Cd2+ had a great influence on adsorption of NP by the strain.As for the effect of living stain,adsorption by P.aeruginosa SH1 cells was stimulated at low concentrations of Cd2+(0.5 mg·L-1),while inhibited at higher levels (≥5 mg·L-1).As for inactivation of microbes,adsorption by cells was stimulated at higher concentrations,but was only slightly influenced at low levels.The results showed that the intracellular enzymes had much greater degradation rate than the living cells.Different concentrations of Cd2+ had different effects on bacteria and intracellular enzyme degradation of NP separately.The degradation efficiency when using intracellular enzymes and bacteria was inhibited at higher levels of Cd2+ and the intracellular enzyme inhibition was more significant.Degradation by cells was stimulated at low concentrations of Cd2+,but no significant impact was found on that by intracellular enzymes.The degradation process of NP by intracellular enzymes of the strain conformed to the first-order kinetic model.The highest reaction rate was achieved when the concentrations of Cd2+ was 0.5 mg·L-1 and the half-life of this substrate was 5.5 h.However,the degradation process of NP by the strain did not conform to the first-order kinetic model.

[Biodegradation of decabromodiphenyl ether by intracellular enzyme obtained from Pseudomonas aeruginosa].

The degradation characteristics of decabromodiphenyl ether (BDE-209) by crude enzyme from Pseudomonas aeruginosa were investigated. The results revealed that the degradation efficiency of the intracellular enzyme excreted from this bacterial strain reached 69.22% after incubation with 1 mg x L(-1) BDE-209 for 12 h. Temperature, pH, enzyme concentration and BDE-209 concentration all influenced the ability of crude enzyme to degrade BDE-209. When the BDE-209 concentration was 1 mg x L(-1), the optimal condition for enzymatic degradation was temperature 30 degrees C and pH 7.5, and the degradation rate increased with increasing enzyme concentration. The degradation process of BDE-209 by intracellular enzyme of the strain conformed to the first-order kinetic model. The highest reaction rate was achieved when the initial concentration of BDE-209 was 1 mg x L(-1) and the half-life of this substrate was 6.9 h. In addition, the biodegradation of BDE-209 can be well described by enzymatic reaction of high concentration substrate inhibition, with a maximum substrate utilization rate of 0.133 mg x (L x h)(-1), a Michaelis-Menten constant of 0.642 mg x L(-1), and an inhibitory constant of 1.558 mg x L(-1), respectively.

[Study of biomechanical properties of mucosa scars after cleft palate surgery].

OBJECTIVE: To explore biomechanical properties and stress-strain of mucosa scars after cleft palate surgery. METHODS: After the model of mucosa scars was made, the mucosa scars and normal mucosa were excised and examined immediately by tensionometry. RESULTS: The mucosa scars after cleft palate surgery were compared with normal mucosa. The Poisson's ratio of mucosa scars and normal mucosa was 0.5 and 0.49, respectively, showing no significant difference between the two groups. The ultimate Young's modulus of mucosa scars was about 24.22 MPa, however, it declined to 3.32 Mpa in normal mucosa. CONCLUSIONS: The mucosa scars after cleft palate surgery are biomechanically weaker than normal mucosa. It can be used for further research, such as maxillary orthognathic surgery, distraction osteogenesis, and orthodontic treatment.

[Three-dimensional finite element analysis for external midface distraction after different osteotomy in patients with cleft lip and palate].

OBJECTIVE: To study three-dimensional finite element analysis for external midface distraction after different osteotomy in patients with cleft lip and palate (CLP). METHODS: Three-dimensional FEM models of Le Fort I, II and III osteotomy in CLP patients were established. External midface distraction were simulated. An anteriorly and inferiorly directed 900 g force was applied to bilateral maxillary arch in directions 30 degrees to the occlusal plane. Biomechanical changes for the maxillary complex were investigated by means of finite element analysis. RESULTS: Maxillary complex was advanced after different osteotomy. Constriction of alveolar crest and palate occurred in Le Fort I osteotomy, but not in Le Fort II and III osteotomy. Clockwise rotation occurred in Le Fort I osteotomy complex. Counterclockwise rotation occurred in Le Fort II and III osteotomy complex. CONCLUSIONS: Three-dimensional finite element research on external midface distraction could provide reference for the preoperative design.

[Biomechanical study of internal midface distraction after different types of maxillary osteotomy in patients with cleft lip and palate].

OBJECTIVE: To investigate the biomechanical changes of internal midface distraction after different types of maxillary osteotomy in patients with cleft lip and palate (CLP). METHODS: 3-D finite element (FEM) analysis was used. 3-D models of Le Fort I, II, III osteotomy and soft tissue were established. Based on the new pattern of internal midface distractor, the distraction of maxillary complex was simulated to advance 10 mm anteriorly. The mechanical change was studied. RESULTS: The maxillary complex in CLP were advanced after distraction. Constriction of alveolar crest and palate occurred in Le Fort I osteotomy, but not in Le Fort II and III osteotomy. The maxillary complex was moved anteriorly en bloc after Le Fort III osteotomy, but some degree of rotation of maxillary complex was observed during the distraction after Le Fort I and II osteotomy. In vertical direction, the maxillary complex had more counterclockwise rotation after Le Fort II osteotomy. CONCLUSION: 3-D FEM analysis can be used for the study of internal distraction. It can reflect the maxillary movement and provide the theory basis for preoperative design.