RESUMO
A diagnostic kit for the determination of IgG antibodies to diphtheria toxin ("Abion", Germany) the use of analytical columns "Abicap" was studied in the specialized laboratory of the Tarasevich State Control Institute with a view to determine the possibility of its official registration in the Russian Federation. The kit was evaluated by the following criteria: the correspondence of the specified properties of the preparation to those actually observed; meeting the norms stated in the documents supplied with the kit and WHO requirements; the presence of advantages in comparison with the enzyme immunoassay. In the course of the trial positive results were obtained on all these points.
Assuntos
Toxina Diftérica/imunologia , Técnicas Imunoenzimáticas/normas , Imunoglobulina G/análise , Testes Sorológicos/normas , Humanos , Kit de Reagentes para Diagnóstico , Padrões de Referência , Reprodutibilidade dos Testes , Federação Russa , Estatística como Assunto , Organização Mundial da SaúdeRESUMO
Investigation of 108 P. aeruginosa strains isolated from various clinical material revealed that 54.63% of them belonged to serogroups 2, 5, 16, 18, 20 and 12.96% to serotype 4. Five strains were polyagglutinated. Monocultures of P. aeruginosa were isolated from 55% of patients, in the majority of cases (31 out of 36) these strains were isolated from the urine. Bacterial associations were more frequently detected in discharge from wounds (11 out of 21), in the material obtained by bronchoscopy (9 out of 15), and in the throat (5 out of 6). Assessment of antibioticograms of the isolated P. aeruginosa strains permitted us characterize polymixin, forte ceftazidime, and biseptol as effective agents.
Assuntos
Pseudomonas aeruginosa/isolamento & purificação , Antibacterianos/farmacologia , Broncoscopia , Humanos , Testes de Sensibilidade Microbiana , Pseudomonas aeruginosa/classificação , Pseudomonas aeruginosa/efeitos dos fármacos , Urina/microbiologia , Ferimentos e Lesões/microbiologiaRESUMO
The data on the approbation of the diagnostic value of the enzyme immunoassay (EIA) system for the determination of diphtheria toxin in the blood sera of diphtheria patients and persons suspected for diphtheria are presented. The EIA system was prepared on the basis of F(ab)2 fractions of purified antidiphtheria antibodies. 240 serum samples from diphtheria and tonsillitis patients and from healthy persons were studied. Diphtheria toxin was determined in all patients with the toxic form of diphtheria and in 41.3% of patients with its localized forms. Blood was taken mainly of the first week of the disease. In healthy persons the results of EIA were negative. Thus, the trial of the assay system in a clinical laboratory showed its good diagnostic effectiveness. The use of this EIA system in medical practice is believed to be quite promising.
Assuntos
Antígenos de Bactérias/sangue , Toxina Diftérica/imunologia , Difteria/diagnóstico , Kit de Reagentes para Diagnóstico , Adolescente , Adulto , Doadores de Sangue , Portador Sadio/diagnóstico , Estudos de Avaliação como Assunto , Feminino , Humanos , Técnicas Imunoenzimáticas/instrumentação , Masculino , Pessoa de Meia-Idade , U.R.S.S.RESUMO
Obtaining antibodies to individual components of Salmonella antigenic complex is highly important for investigations aimed at the study of the antigenic structure of bacteria, their serological identification and the development of diagnostic preparations. The method of obtaining antibodies by the oxidation of Salmonella antigens with sodium periodate and creating immunosorbents based on these antibodies with subsequent affinity chromatography has been developed. Monospecific antibodies thus obtained (O2, O4, O9) have been studied and used as monospecific preparations in the agglutination test, the immunofluorescence test and the immunosorbent assay. The development of methods for stabilizing these preparations, thus ensuring their wide practical use, may be of interest.
Assuntos
Anticorpos Antibacterianos/isolamento & purificação , Anticorpos Monoclonais/isolamento & purificação , Especificidade de Anticorpos , Cromatografia de Afinidade/métodos , Salmonella/imunologia , Testes de Aglutinação , Animais , Anticorpos Antibacterianos/análise , Anticorpos Monoclonais/análise , Antígenos de Bactérias/imunologia , Imunofluorescência , Técnicas Imunoenzimáticas , Antígenos O , Coelhos , Salmonella paratyphi A/imunologiaRESUMO
A variant of the immunoenzyme assay, reducing the duration of the assay procedure by 3 hours in comparison with the standard technique, is presented. This rapid variant is sufficiently sensitive and reproducible.