Plant diversity decreases greenhouse gas emissions by increasing soil and plant carbon storage in terrestrial ecosystems.

The decline in global plant diversity has raised concerns about its implications for carbon fixation and global greenhouse gas emissions (GGE), including carbon dioxide (CO2), nitrous oxide (N2O) and methane (CH4). Therefore, we conducted a comprehensive meta-analysis of 2103 paired observations, examining GGE, soil organic carbon (SOC) and plant carbon in plant mixtures and monocultures. Our findings indicate that plant mixtures decrease soil N2O emissions by 21.4% compared to monocultures. No significant differences occurred between mixtures and monocultures for soil CO2 emissions, CH4 emissions or CH4 uptake. Plant mixtures exhibit higher SOC and plant carbon storage than monocultures. After 10 years of vegetation development, a 40% reduction in species richness decreases SOC content and plant carbon storage by 12.3% and 58.7% respectively. These findings offer insights into the intricate connections between plant diversity, soil and plant carbon storage and GGE-a critical but previously unexamined aspect of biodiversity-ecosystem functioning.

Overexpression of BnaA10.WRKY75 Decreases Cadmium and Salt Tolerance via Increasing ROS Accumulation in Arabidopsis and Brassica napus L.

Soil is indispensable for agricultural production but has been seriously polluted by cadmium and salt in recent years. Many crops are suffering from this, including rapeseed, the third largest global oilseed crop. However, genes simultaneously related to both cadmium and salt stress have not been extensively reported yet. In this study, BnaA10.WRKY75 was screened from previous RNA-seq data related to cadmium and salt stress and further analyses including sequence comparison, GUS staining, transformation and qRT-PCR were conducted to confirm its function. GUS staining and qRT-PCR results indicated BnaA10.WRKY75 was induced by CdCl2 and NaCl treatment. Sequence analysis suggested BnaA10.WRKY75 belongs to Group IIc of the WRKY gene family and transient expression assay showed it was a nuclear localized transcription factor. BnaA10.WRKY75-overexpressing Arabidopsis and rapeseed plants accumulated more H2O2 and O2- and were more sensitive to CdCl2 and NaCl treatment compared with untransformed plants, which may be caused by the downregulation of BnaC03.CAT2. Our study reported that BnaA10.WRKY75 increases sensitivity to cadmium and salt stress by disrupting the balance of reactive oxygen species both in Arabidopsis and rapeseed. The results support the further understanding of the mechanisms underlying cadmium and salt tolerance and provide BnaA10.WRKY75 as a valuable gene for rapeseed abiotic stress breeding.

Enhancing zinc biofortification and mitigating cadmium toxicity in soil-earthworm-spinach systems using different zinc sources.

Cadmium (Cd) pollution poses significant threats to soil organisms and human health by contaminating the food chain. This study aimed to assess the impact of various concentrations (50, 250, and 500 mg·kg-1) of zinc oxide nanoparticles (ZnO NPs), bulk ZnO, and ZnSO4 on morphological changes and toxic effects of Cd in the presence of earthworms and spinach. The results showed that Zn application markedly improved spinach growth parameters (such as fresh weight, plant height, root length, and root-specific surface area) and root morphology while significantly reducing Cd concentration and Cd bioconcentration factors (BCF-Cd) in spinach and earthworms, with ZnO NPs exhibiting the most pronounced effects. Earthworm, spinach root, and shoot Cd concentration decreased by 82.3 %, 77.0 %, and 75.6 %, respectively, compared to CK. Sequential-step extraction (BCR) analysis revealed a shift in soil Cd from stable to available forms, consistent with the available Cd (DTPA-Cd) results. All Zn treatments significantly reduced Cd accumulation, alleviated Cd-induced stress, and promoted spinach growth, with ZnO NPs demonstrating the highest Cd reduction and Zn bioaugmentation efficiencies compared to bulk ZnO and ZnSO4 at equivalent concentrations. Therefore, ZnO NPs offer a safer and more effective option for agricultural production and soil heavy metal pollution management than other Zn fertilizers.

Bromine contamination and risk management in terrestrial and aquatic ecosystems.

Bromine (Br) is widely distributed through the lithosphere and hydrosphere, and its chemistry in the environment is affected by natural processes and anthropogenic activities. While the chemistry of Br in the atmosphere has been comprehensively explored, there has never been an overview of the chemistry of Br in soil and aquatic systems. This review synthesizes current knowledge on the sources, geochemistry, health and environmental threats, remediation approaches, and regulatory guidelines pertaining to Br pollution in terrestrial and aquatic environments. Volcanic eruptions, geothermal streams, and seawater are the major natural sources of Br. In soils and sediments, Br undergoes natural cycling between organic and inorganic forms, with bromination reactions occurring both abiotically and through microbial activity. For organisms, Br is a non-essential element; it is passively taken up by plant roots in the form of the Br- anion. Elevated Br- levels can limit plant growth on coastal soils of arid and semi-arid environments. Br is used in the chemical industry to manufacture pesticides, flame retardants, pharmaceuticals, and other products. Anthropogenic sources of organobromine contaminants in the environment are primarily wastewater treatment, fumigants, and flame retardants. When aqueous Br- reacts with oxidants in water treatment plants, it can generate brominated disinfection by-products (DBPs), and exposure to DBPs is linked to adverse human health effects including increased cancer risk. Br- can be removed from aquatic systems using adsorbents, and amelioration of soils containing excess Br- can be achieved by leaching, adding various amendments, or phytoremediation. Developing cost-effective methods for Br- removal from wastewater would help address the problem of toxic brominated DBPs. Other anthropogenic organobromines, such as polybrominated diphenyl ether (PBDE) flame retardants, are persistent, toxic, and bioaccumulative, posing a challenge in environmental remediation. Future research directives for managing Br pollution sustainably in various environmental settings are suggested here.

Diversifying crop rotation increases food production, reduces net greenhouse gas emissions and improves soil health.

Global food production faces challenges in balancing the need for increased yields with environmental sustainability. This study presents a six-year field experiment in the North China Plain, demonstrating the benefits of diversifying traditional cereal monoculture (wheat-maize) with cash crops (sweet potato) and legumes (peanut and soybean). The diversified rotations increase equivalent yield by up to 38%, reduce N2O emissions by 39%, and improve the system's greenhouse gas balance by 88%. Furthermore, including legumes in crop rotations stimulates soil microbial activities, increases soil organic carbon stocks by 8%, and enhances soil health (indexed with the selected soil physiochemical and biological properties) by 45%. The large-scale adoption of diversified cropping systems in the North China Plain could increase cereal production by 32% when wheat-maize follows alternative crops in rotation and farmer income by 20% while benefiting the environment. This study provides an example of sustainable food production practices, emphasizing the significance of crop diversification for long-term agricultural resilience and soil health.

Uncovering the transcriptional responses of tobacco (Nicotiana tabacum L.) roots to Ralstonia solanacearum infection: a comparative study of resistant and susceptible cultivars.

BACKGROUND: Tobacco bacterial wilt (TBW) caused by Ralstonia solanacearum is the most serious soil-borne disease of tobacco that significantly reduces crop yield. However, the limited availability of resistance in tobacco hinders breeding efforts for this disease. RESULTS: In this study, we conducted hydroponic experiments for the root expression profiles of D101 (resistant) and Honghuadajinyuan (susceptible) cultivars in response to BW infection at 0 h, 6 h, 1 d, 3 d, and 7d to explore the defense mechanisms of BW resistance in tobacco. As a result, 20,711 and 16,663 (total: 23,568) differentially expressed genes (DEGs) were identified in the resistant and susceptible cultivars, respectively. In brief, at 6 h, 1 d, 3 d, and 7 d, the resistant cultivar showed upregulation of 1553, 1124, 2583, and 7512 genes, while the susceptible cultivar showed downregulation of 1213, 1295, 813, and 7735 genes. Similarly, across these time points, the resistant cultivar had downregulation of 1034, 749, 1686, and 11,086 genes, whereas the susceptible cultivar had upregulation of 1953, 1790, 2334, and 6380 genes. The resistant cultivar had more up-regulated genes at 3 d and 7 d than the susceptible cultivar, indicating that the resistant cultivar has a more robust defense response against the pathogen. The GO and KEGG enrichment analysis showed that these genes are involved in responses to oxidative stress, plant-pathogen interactions, cell walls, glutathione and phenylalanine metabolism, and plant hormone signal transduction. Among the DEGs, 239 potential candidate genes were detected, including 49 phenylpropane/flavonoids pathway-associated, 45 glutathione metabolic pathway-associated, 47 WRKY, 48 ERFs, eight ARFs, 26 pathogenesis-related genes (PRs), and 14 short-chain dehydrogenase/reductase genes. In addition, two highly expressed novel genes (MSTRG.61386-R1B-17 and MSTRG.61568) encoding nucleotide-binding site leucine-rich repeat (NBS-LRR) proteins were identified in both cultivars at 7 d. CONCLUSIONS: This study revealed significant enrichment of DEGs in GO and KEGG terms linked to glutathione, flavonoids, and phenylpropane pathways, indicating the potential role of glutathione and flavonoids in early BW resistance in tobacco roots. These findings offer fundamental insight for further exploration of the genetic architecture and molecular mechanisms of BW resistance in tobacco and solanaceous plants at the molecular level.

Mitigating greenhouse gas emissions by replacing inorganic fertilizer with organic fertilizer in wheat-maize rotation systems in China.

Combining organic and inorganic fertilizer applications can help reduce inorganic fertilizer use and increase soil fertility. However, the most suitable proportion of organic fertilizer is unknown, and the effect of combining organic and inorganic fertilizers on greenhouse gas (GHG) emissions is inconclusive. This study aimed to identify the optimum ratio of inorganic fertilizer to organic fertilizer in a winter wheat-summer maize cropping system in northern China to achieve high grain yields and low GHG intensities. The study compared six fertilizer treatments: no fertilization (CK), conventional inorganic fertilization (NP), and constant total nitrogen input with 25% (25%OF), 50% (50%OF), 75% (75%OF), or 100% (100%OF) organic fertilizer. The results showed that the 75%OF treatment increased the winter wheat and summer maize yields the most, by 7.2-25.1% and 15.3-16.7%, respectively, compared to NP. The 75%OF and 100%OF treatments had the lowest nitrous oxide (N2O) emissions, 187.3% and 200.2% lower than the NP treatment, while all fertilizer treatments decreased methane (CH4) absorption (by 33.1-82.0%) compared to CK. Carbon dioxide flux increased in the summer maize growing season (by 7.7-30.5%) compared to CK but did not significantly differ between fertilizer treatments. The average global warming potential (GWP) rankings across two wheat-maize rotations were NP > 50%OF > 25%OF > 100%OF > 75%OF > CK, and greenhouse gas intensity (GHGI) rankings were NP > 25%OF > 50%OF > 100%OF > 75%OF > CK. We recommend using 75% organic fertilizer/25% inorganic fertilizer to reduce GHG emissions and ensure high crop yields in wheat-maize rotation systems in northern China.

Sustainable management of hazardous asbestos-containing materials: Containment, stabilization and inertization.

Asbestos is a group of six major silicate minerals that belong to the serpentine and amphibole families, and include chrysotile, amosite, crocidolite, anthophyllite, tremolite and actinolite. Weathering and human disturbance of asbestos-containing materials (ACMs) can lead to the emission of asbestos dust, and the inhalation of respirable asbestos fibrous dust can lead to 'mesothelioma' cancer and other diseases, including the progressive lung disease called 'asbestosis'. There is a considerable legacy of in-situ ACMs in the built environment, and it is not practically or economically possible to safely remove ACMs from the built environment. The aim of the review is to examine the three approaches used for the sustainable management of hazardous ACMs in the built environment: containment, stabilization, and inertization or destruction. Most of the asbestos remaining in the built environment can be contained in a physically secured form so that it does not present a significant health risk of emitting toxic airborne fibres. In settings where safe removal is not practically feasible, stabilization and encapsulation can provide a promising solution, especially in areas where ACMs are exposed to weathering or disturbance. Complete destruction and inertization of asbestos can be achieved by thermal decomposition using plasma and microwave radiation. Bioremediation and chemical treatment (e.g., ultrasound with oxalic acid) have been found to be effective in the inertization of ACMs. Technologies that achieve complete destruction of ACMs are found to be attractive because the treated products can be recycled or safely disposed of in landfills.

Enhanced nodulation and phosphorus acquisition from sparingly-soluble iron phosphate upon treatment with arbuscular mycorrhizal fungi in chickpea.

The coordination/trade-off among below-ground strategies for phosphorus (P) acquisition, including root morphology, carboxylate exudation and colonisation by arbuscular mycorrhizal fungi (AMF), is not well understood. This is the first study investigating the relationships between root nodulation, morphology, carboxylates and colonisation by an indigenous community of AMF under varying P levels and source. Two chickpea genotypes with contrasting amounts of rhizosheath carboxylates were grown in pots at six P levels (from 0 to 160 µg g-1 ) as KH2 PO4 (KP, highly soluble) or FePO4 (FeP, sparingly soluble), with or without AMF (±AMF) treatment. Under both FeP and KP, the presence of AMF inhibited shoot growth and shoot branching, decreased total root length and specific root length, increased mean root diameter and root tissue density and reduced carboxylates. However, the role of AMF in acquiring P differed between the two P sources, with the enhanced P acquisition under FeP while not under KP. Co-inoculation of AMF and rhizobia enhanced nodulation under FeP, but not under KP. Our results suggest that the effects of AMF on shoot branching were mediated by cytokinins as the reduced shoot branching in FeP40 and KP40 under +AMF relative to -AMF coincided with a decreased concentration of cytokinins in xylem sap for both genotypes.

Beryllium contamination and its risk management in terrestrial and aquatic environmental settings.

Beryllium (Be) is a relatively rare element and occurs naturally in the Earth's crust, in coal, and in various minerals. Beryllium is used as an alloy with other metals in aerospace, electronics and mechanical industries. The major emission sources to the atmosphere are the combustion of coal and fossil fuels and the incineration of municipal solid waste. In soils and natural waters, the majority of Be is sorbed to soil particles and sediments. The majority of contamination occurs through atmospheric deposition of Be on aboveground plant parts. Beryllium and its compounds are toxic to humans and are grouped as carcinogens. The general public is exposed to Be through inhalation of air and the consumption of Be-contaminated food and drinking water. Immobilization of Be in soil and groundwater using organic and inorganic amendments reduces the bioavailability and mobility of Be, thereby limiting the transfer into the food chain. Mobilization of Be in soil using chelating agents facilitates their removal through soil washing and plant uptake. This review provides an overview of the current understanding of the sources, geochemistry, health hazards, remediation practices, and current regulatory mandates of Be contamination in complex environmental settings, including soil and aquatic ecosystems.

Adsorption and immobilization performance of pine-cone pristine and engineered biochars for antimony in aqueous solution and military shooting range soil: An integrated novel approach.

Antimony (Sb-V), a carcinogenic metalloid, is becoming prevalent in water and soil due to anthropogenic activities. Biochar could be an effective remedy for Sb(V)-contaminated water and soil. In this study, we used pristine and engineered pinecone-derived biochar as an innovative approach for treating Sb(V)-contaminated water and shooting range soil. Biochar was produced from pine-cone waste (pristine biochar) and enriched with Fe and Al salts via saturation (engineered biochar). Adsorption tests in water revealed that iron-modified biochar showed higher adsorption capacity (8.68 mg g-1) than that of the pristine biochar (2.49 mg g-1) and aluminum-modified biochar (3.40 mg g-1). Isotherm and kinetic modeling of the adsorption data suggested that the adsorption process varied from monolayer to multilayer, with chemisorption as the dominant interaction mechanism between Sb(V) and the biochars. The post-adsorption study of iron-modified biochar by Fourier Transform Infrared (FTIR) and X-ray diffraction (XRD) further supported the chemical bonding and outer-sphere complexation of Sb(V) with Fe, N-H, O-H, C-O and CC components. The pristine and iron-modified biochars also successfully immobilized Sb(V) in a shooting range soil, more so in the latter. Subsequent sequential extractions and post-analysis by scanning electron microscopy (SEM), energy dispersive X-ray analysis (EDX), and elemental dot mapping revealed that Sb in the treated soil transformed to a more stable form. It was concluded that iron-modified biochar could act as an efficient material for the adsorption and immobilization of Sb(V) in water and soil, respectively.

Transcriptional and biochemical profiling of defense enzymes in Citrus sinensis during salicylic acid and cinnamon mediated suppression of green and blue mold.

Green and blue mold of citrus are threatening diseases that continuously inflict economic post-harvest loss. The suppressive effect of salicylic (SA) and Cinnamomum verum (CV) on green and blue mold of sweet oranges was investigated in this study. Among five tested plant extracts methanolic extract of Cinnamon caused the highest colony growth inhibition of P. digitatum and P. italicum in an in vitro antifungal assay. The methanolic extract of Cinnamon in combination with SA showed the lowest disease incidence and severity of green and blue mold on citrus fruit without affecting the fruit quality. Transcriptional profiling of defense enzymes revealed that the polyphenol oxidase (PPO), phenylalanine ammonia-lyase (PAL), and peroxidase (POD) genes were upregulated in fruit treated with CV, SA, and their combination compared to the control. The treatment SA+CV caused the highest upsurge in PPO, POD, and PAL gene expression than the control. Furthermore, the biochemical quantification of PPO, POD and PAL also revealed a similar pattern of activity. The present findings unravel the fact that the escalation in the activity of tested defense enzymes is possibly associated with the reduced incidence of blue and green molds. In conclusion, the study unveils the promising suppressive potential of SA+CV against green and blue mold by regulating the expression of PPO, POD, and PAL genes. Therefore, these treatments can find a role as safer alternatives to chemicals in the management of post-harvest green and blue mold.

Seed Endophyte bacteria enhance drought stress tolerance in Hordeum vulgare by regulating, physiological characteristics, antioxidants and minerals uptake.

Growth stimulating bacteria help remediate dry arid soil and plant stress. Here, Pseudomonas sp. and Pantoea sp. we used to study the stress ecology of Hordeum vulgare and the environmental impact of water deficit on soil characteristics, growth, photosynthesis apparatus, mineral acquisition and antioxidiant defense. Plants inoculated with Pseudomonas or Pantoea had significantly higher (about 2 folds) soil carbon flux (soil respiration), chlorophyll levels (18%), net photosynthetic rate (33% in Pantoea and 54% in Pseudomonas), (44%) stomatal conductance than uninoculated plants in stressed conditions. Both bacterial strains improved leaf growth (23-29%) and root development under well-watered conditions but reduced around (25%) root biomass under drought. Plants inoculated with Pseudomonas or Pantoea under drought also increased of about 27% leaf respiration and transpiration (48%) but decreased water use efficiency, photoinhibition (91%), and the risk of oxidative stress (ETR/A) (49%). Drought stress increased most of the studied antioxidant enzymatic activities in the plants inoculated with Pseudomonas or Pantoea, which reduce the membrane damage and protect plants form oxidative defenses. Drought stress increased K+ acquisition around 50% in both shoots inoculated with Pseudomonas or Pantoea relative to non-stressed plants. Plants inoculated with Pseudomonas or Pantoea increased shoot Na+ while root Na+ only increased in plants inoculated with Pseudomonas in stressed conditions. Drought stress increased shoot Mg2+ in plants inoculated with Pseudomonas or Pantoea but did not affect Ca2+ relative to non-stressed plants. Drought stress increased about 70% K+/Na+ ratio only in plants inoculated with Pseudomonas relative to non-stressed plants. Our results indicate that inoculating barley with the studied bacterial strains increases plant biomass and can therefore play a role in the environmental remediation of drylands for food production.

Salicylic Acid Priming Regulates Stomatal Conductance, Trichome Density and Improves Cadmium Stress Tolerance in Mentha arvensis L.

The application of phytohormones through seed priming could enhance quality of important medicinal and aromatic plants (MAPs) under heavy metal stress. We evaluated the potential of salicylic acid (SA) priming for overcoming the adverse effects of cadmium stress in Mentha arvensis L. plants. Suckers of plants were primed with SA before transplanting them into soil. At 30 days after transplanting, two doses (50 and 100 µm) of CdCl2 were applied to the soil. Both Cd treatments altered plant growth, photosynthetic pigments, leaf gas exchange attributes, and mineral nutrient contents. The 50 and 100 µm Cd treatments increased endogenous Cd content by 97.95 and 98.03%, electrolyte leakage (EL) by 34.21 and 44.38%, hydrogen peroxide (H2O2) by 34.71 and 55.80%, malondialdehyde (MDA) by 53.08 and 63.15%, and superoxide content (O2 -•) by 24.07 and 38.43%, respectively. Cd triggered the up-regulation of antioxidant enzyme activities (superoxide dismutase, SOD; catalase, CAT; ascorbate peroxidase, APX; and glutathione reductase GR) and increased osmolyte biosynthesis and, interestingly, secondary metabolite (SM) accumulation. The presence of SA and Cd had an additive effect on these parameters. Nevertheless, plants primed with SA regulated stomatal conductance under Cd stress. SA priming to menthol mint plants under Cd stress overcome the effects of Cd stress while increasing SMs.

Genome-wide identification and development of InDel markers in tobacco (Nicotiana tabacum L.) using RAD-seq.

Insertions and deletions (InDels) can be used as molecular markers in genetic studies and marker-assisted selection breeding. However, genetic improvement in tobacco has been hindered by limited genetic diversity information and relatedness within available germplasm. A Chinese tobacco variety, Yueyan-98, was resequenced using restriction-site associated DNA (RAD-seq) approach to develop InDel markers. In total, 32,884 InDel loci were detected between Yueyan-98 and the K326 reference sequence [18,598 (56.55%) deletions and 14,288 (43.45%) insertions], ranging from 1 to 62 bp in length. Of the 6,733 InDels (> 4 bp) that were suitable for polyacrylamide gel electrophoresis, 150 were randomly selected. These 150 InDels were unevenly distributed on 23 chromosomes, and the highest numbers of InDels were observed on chromosomes Nt05, Nt13, and Nt23. The average density of adjacent InDels was 19.36 Mb. Thirty-seven InDels were located in genic regions. Polymerase chain reaction (PCR)-based markers were developed to validate polymorphism; 113 (79.80%) of the 150 InDel markers showed polymorphism and were further used for genetic diversity analysis of 50 tobacco accessions (13 from China, 1 from Mexico, and 36 from the USA). The average expected heterozygosity (He) and polymorphism information content (PIC) values were 0.28 ± 0.16 and 0.38 ± 0.10, respectively. The average Shannon diversity index (I) was 0.34 ± 0.18, with genetic diversity ranging from 0.13-0.57. The 50 accessions were classified into two groups with a genetic similarity coefficient of 0.68. Principal coordinate analysis (PCoA) and population structure analysis showed similar results and divided the population into two groups unrelated to their geographical origins. AMOVA showed 4% variance among the population and the remaining 96% within the population, suggesting low genetic differentiation between two subpopulations. Furthermore, 10 InDels (19 alleles) were significantly identified for tobacco plant height using GLM+Q model at P < 0.005. Among these, three markers (Nt-I-26, Nt-I-41, and Nt-I-44) were detected in at least two environments, with phenotypic variance explained (PVE) ranging from 14.03 to 32.68%. The polymorphic InDel markers developed can be used for hybrid identification, genetic diversity, genetic linkage map construction, gene mapping, and MAS breeding programs of tobacco. Supplementary Information: The online version contains supplementary material available at 10.1007/s12298-022-01187-3.

Application of humic acid and biofertilizers changes oil and phenolic compounds of fennel and fenugreek in intercropping systems.

The study investigated the effect of organic/biofertilizers in intercropping patterns on seed yield and yield components and essential oil, fatty acid, and phenolic compounds of fennel (Foeniculum vulgare L.) and fenugreek (Trigonella foenum-graecum L.). Experimental treatments included the application of humic acid (HA), biofertilizers (BFS), and the unfertilized control in five planting patterns [1 row fennel + 2 rows fenugreek intercropping (1F:2FG), 2 rows fennel + 2 rows fenugreek intercropping (2F:2FG), 2 rows fennel + 4 rows fenugreek intercropping (2F:4FG), and sole cropping of each species]. Sole cropping with BFS produced the highest seed yields for fennel (2233 kg ha-1) and fenugreek (1240 kg ha-1). In contrast, the 2F:2FG intercropping ratio with BFS yielded the maximum fixed oil content for fennel (17.4%) and fenugreek (8.3%). Application of HA and BFS enhanced oil yields by 66% and 75% in fennel and 40% and 57% in fenugreek, respectively. The 2F:2FG intercropping ratio with BFS produced the maximum essential oil constituents [(E)-anethole, estragole, and fenchone] in fennel. In addition, 2F:4FG with BFS and 1F:1FG with HA produced the highest unsaturated fatty acid (oleic and linoleic acids) concentration in both species. The 2F:2FG intercropping ratio with BFS and HA produced the highest chlorogenic acid and quercetin contents, respectively, in fennel. In contrast, the 2F:4FG intercropping ratio with HA produced the highest chlorogenic acid and caffeic acid contents in fenugreek. Intercropping fennel/fenugreek with BFS or HA improved the essential oil content (fennel only), fixed oil quality and quantity, and phenolic compounds and created a more sustainable cultivation system than sole cropping systems for both species under low-input conditions.

Beijerinckia fluminensis BFC-33, a novel multi-stress-tolerant soil bacterium: Deciphering the stress amelioration, phytopathogenic inhibition and growth promotion in Triticum aestivum (L.).

Environmental challenges like drought, salinity, heavy metals and pesticides directly/indirectly influence the environment and decreased the agricultural output. During its long developmental stages, cereal crops including wheat is exposed to a variety of abiotic/biotic stressors. Certain beneficial soil bacteria that can ameliorate environmental stresses can be exploited as crop growth promoters/enhancers under adverse situations. In this study, Beijerinckia fluminensis BFC-33 (accession no. MT672580) isolated from potato rhizosphere tolerated variousabiotic (drought, salinity, temperature, heavy metals, and fungicides) stresses. Strain BFC-33 demonstrated multifarious plant-growth-promoting (PGP) characteristics, such as the production of indole-3-acetic acid, P-solubilization, ACC deaminase, ammonia, siderophore, HCN, EPS, and extracellular enzymes. The antagonistic potential of BFC-33 against major fungal pathogens was ranked: Alternaria alternata (79.2%)>Rhizoctonia solani (69%)>Fusarium oxysporum (23.5%)>Ustilaginoidea virens (17%). Furthermore, bacterization of wheat seeds witha multi-stress-tolerant strain revealed B. fluminensis as a plant growth enhancer and biocontrol agent. For instance, increase in root length (cm) in BFC-33 inoculated wheat exposed to abiotic and biotic stresses at the seedling stage was ranked: B. fluminensis (24.2)>B. fluminensis + 100µgTBZLmL-1 (21.3) = B. fluminensis + 2%PEG (21.3)>B. fluminensis + 100 mM NaCl (19.7)>B. fluminensis + 100µgPbmL-1 (19) = B. fluminensis 100µgMNZBmL-1 (19)>B. fluminensis + A. alternata (17.4)>B. fluminensis + 100µgCdmL-1 (17)>B. fluminensis + F. oxysporum (13.4). In addition, increase in carotenoid accumulation (mg g-1FW) in the foliage of BFC-33 inoculated wheat exposed to fungal infection was ranked: BFC-33 (3.88)>BFC-33+ A. alternata (3.0)>BFC-33+ R. solani (2.78)>BFC-33+ F. oxysporum (2.44). Moreover, BFC-33 inoculation significantly (p ≤ 0.05) reduced stress-induced stressor molecules (proline and TBARS) and electrolyte leakage. Furthermore, B. fluminensis BFC-33 potentially enhanced the defense responses in wheat seedlings by increasing phenylalanine ammonia lyase (PAL), ß-1,3 glucanase, and polyphenol oxidase (PPO), which play a significant role in protecting plants from phytopathogens. Even so, by successfully establishing a product with the requisite effects under field settings, selecting multi-stress-tolerant and antagonistic plant growth promoting rhizobacteria (PGPRs) would be helpful to end-users. Future use of native multi-stress-tolerant bacteria as biocontrol agents in conjunction with existing drought, salinity, heavy metal, and pesticide tolerance might contribute to global food security.

Differential Physio-Biochemical and Metabolic Responses of Peanut (Arachis hypogaea L.) under Multiple Abiotic Stress Conditions.

The frequency and severity of extreme climatic conditions such as drought, salinity, cold, and heat are increasing due to climate change. Moreover, in the field, plants are affected by multiple abiotic stresses simultaneously or sequentially. Thus, it is imperative to compare the effects of stress combinations on crop plants relative to individual stresses. This study investigated the differential regulation of physio-biochemical and metabolomics parameters in peanut (Arachis hypogaea L.) under individual (salt, drought, cold, and heat) and combined stress treatments using multivariate correlation analysis. The results showed that combined heat, salt, and drought stress compounds the stress effect of individual stresses. Combined stresses that included heat had the highest electrolyte leakage and lowest relative water content. Lipid peroxidation and chlorophyll contents did not significantly change under combined stresses. Biochemical parameters, such as free amino acids, polyphenol, starch, and sugars, significantly changed under combined stresses compared to individual stresses. Free amino acids increased under combined stresses that included heat; starch, sugars, and polyphenols increased under combined stresses that included drought; proline concentration increased under combined stresses that included salt. Metabolomics data that were obtained under different individual and combined stresses can be used to identify molecular phenotypes that are involved in the acclimation response of plants under changing abiotic stress conditions. Peanut metabolomics identified 160 metabolites, including amino acids, sugars, sugar alcohols, organic acids, fatty acids, sugar acids, and other organic compounds. Pathway enrichment analysis revealed that abiotic stresses significantly affected amino acid, amino sugar, and sugar metabolism. The stress treatments affected the metabolites that were associated with the tricarboxylic acid (TCA) and urea cycles and associated amino acid biosynthesis pathway intermediates. Principal component analysis (PCA), partial least squares-discriminant analysis (PLS-DA), and heatmap analysis identified potential marker metabolites (pinitol, malic acid, and xylopyranose) that were associated with abiotic stress combinations, which could be used in breeding efforts to develop peanut cultivars that are resilient to climate change. The study will also facilitate researchers to explore different stress indicators to identify resistant cultivars for future crop improvement programs.

Antimony contamination and its risk management in complex environmental settings: A review.

Antimony (Sb) is introduced into soils, sediments, and aquatic environments from various sources such as weathering of sulfide ores, leaching of mining wastes, and anthropogenic activities. High Sb concentrations are toxic to ecosystems and potentially to public health via the accumulation in food chain. Although Sb is poisonous and carcinogenic to humans, the exact mechanisms causing toxicity still remain unclear. Most studies concerning the remediation of soils and aquatic environments contaminated with Sb have evaluated various amendments that reduce Sb bioavailability and toxicity. However, there is no comprehensive review on the biogeochemistry and transformation of Sb related to its remediation. Therefore, the present review summarizes: (1) the sources of Sb and its geochemical distribution and speciation in soils and aquatic environments, (2) the biogeochemical processes that govern Sb mobilization, bioavailability, toxicity in soils and aquatic environments, and possible threats to human and ecosystem health, and (3) the approaches used to remediate Sb-contaminated soils and water and mitigate potential environmental and health risks. Knowledge gaps and future research needs also are discussed. The review presents up-to-date knowledge about the fate of Sb in soils and aquatic environments and contributes to an important insight into the environmental hazards of Sb. The findings from the review should help to develop innovative and appropriate technologies for controlling Sb bioavailability and toxicity and sustainably managing Sb-polluted soils and water, subsequently minimizing its environmental and human health risks.

Identification of Novel Quantitative Trait Nucleotides and Candidate Genes for Bacterial Wilt Resistance in Tobacco (Nicotiana tabacum L.) Using Genotyping-by-Sequencing and Multi-Locus Genome-Wide Association Studies.

Tobacco bacterial wilt (TBW) is a devastating soil-borne disease threatening the yield and quality of tobacco. However, its genetic foundations are not fully understood. In this study, we identified 126,602 high-quality single-nucleotide polymorphisms (SNPs) in 94 tobacco accessions using genotyping-by-sequencing (GBS) and a 94.56 KB linkage disequilibrium (LD) decay rate for candidate gene selection. The population structure analysis revealed two subpopulations with 37 and 57 tobacco accessions. Four multi-locus genome-wide association study (ML-GWAS) approaches identified 142 quantitative trait nucleotides (QTNs) in E1-E4 and the best linear unbiased prediction (BLUP), explaining 0.49-22.52% phenotypic variance. Of these, 38 novel stable QTNs were identified across at least two environments/methods, and their alleles showed significant TBW-DI differences. The number of superior alleles associated with TBW resistance for each accession ranged from 4 to 24; eight accessions had more than 18 superior alleles. Based on TBW-resistant alleles, the five best cross combinations were predicted, including MC133 × Ruyuan No. 1 and CO258 × ROX28. We identified 52 candidate genes around 38 QTNs related to TBW resistance based on homologous functional annotation and KEGG enrichment analysis, e.g., CYCD3;2, BSK1, Nitab4.5_0000641g0050, Nitab4.5_0000929g0030. To the best of our knowledge, this is the first comprehensive study to identify QTNs, superior alleles, and their candidate genes for breeding TBW-resistant tobacco varieties. The results provide further insight into the genetic architecture, marker-assisted selection, and functional genomics of TBW resistance, improving future breeding efforts to increase crop productivity.