Estresse percebido e sofrimento psíquico em gestoresde saúde na pandemia da Covid-19 / Perceived stress and psychic suffering in healthmanagers in the Covid-19 pandemic

Objetivo: analisar o nível de estresse percebido e sofrimento psíquico em gestores de saúde na pandemia da Covid-19. Método: estudo descritivo, transversal com abordagem quantitativa. A coleta ocorreu de abril a setembro de 2021, com 40 gestores de serviço de saúde. Utilizou-se para a coleta de dados um instrumento para caracterização sociodemográfica e ocupacional e as escalas "Perceived Stress Scale-14" e "Self Reporting Questionnaire" para avaliação do estresse percebido e sofrimento psíquico. Os dados coletados foram analisados no Statistical Package for the Social Sciences versão 22.0. O presente estudo faz parte de um projeto intitulado "Trabalhadores dos Serviços de Saúde Frente à Pandemia de Covid-19", aprovado pelo Comitê de Ética em Pesquisa sob CAAE número 35260620.9.0000.5231. Resultados: a maioria dos profissionais eram do sexo feminino (90%, N=36), casados (70%, N=28), com filhos (80%, N=32), com média de idade de 45 anos e com pós--graduação (47,5%, N=19). A média dos escores relacionados ao estresse percebido foi 31,13 pontos (DP=3,77) sendo o mínimo 24 e máximo de 42 pontos. Com relação ao sofrimento psíquico, (40%, N=16) os gestores apresentaram prováveis casos de transtornos. A prática de atividades físicas e de lazer (p<0,05) tem papel importante na diminuição do estresse percebido e do sofrimento psíquico. Conclusão: os gestores em saúde apresentaram, durante a pandemia, estresse e sofrimento psíquico, resultados esses que devem ser considerados para promoção de autocuidado aos gestores de saúde, enfatizando a necessidade da realização de atividades físicas e de lazer.

Objective: to analyze the level of perceived stress and psychological suffering in health managers during the Covid-19 pandemic. Method: descriptive, cross-sectional study with a quantitative approach. The collection took place from April to September 2021, with 40 health service managers. An instrument for socio-demographic and occupational characterization and the "Perceived Stress Scale-14" and "Self Reporting Questionnaire" scales were used for the assessment of perceived stress and psychic suffering. The collected data were analyzed using the Statistical Package for the Social Sciences version 22.0. The present study is part of a project entitled "Health Service Workers in the Face of the Covid-19 Pandemic", approved by the Research Ethics Committee under CAAE number 35260620.9.0000.5231. Results: most professionals were female (90%, N=36), married (70%, N=28), with children (80%, N=32), with a mean age of 45 years and with a postgraduate degree. -graduation (47.5%, N=19). The average score related to perceived stress was 31.13 points (SD=3.77), with a minimum of 24 and a maximum of 42 points. With regard to psychic suffering, (40%, N=16) the managers presented probable cases of disorders. The practice of physical and leisure activities (p<0.05) plays an important role in reducing perceived stress and psychological distress. Conclusion: health managers presented, during the pandemic, stress and psychic suffering, results that should be considered for promoting self-care to health managers, emphasizing the need to carry out physical and leisure activities.Keywords: Health manager; Occupational stress; Covid-19; Coronavirus infections.

Bioguided Fractionation of Phyllanthus spp.: Unveiling Anticancer Potential through Metabolomic Correlation and ADMETox Insights.

Cancer remains a significant global health concern, with mortality rates steadily rising and prompting an urgent search for effective treatments. This study focuses on the medicinal properties of plants from the Phyllanthus genus, specifically Phyllanthus amarus and Phyllanthus niruri, which have shown promise in traditional medicine. Through bioguided fractionation using preparative high-performance liquid chromatography (HPLC), bioactive compounds were isolated and identified using ultra-performance liquid chromatography coupled to time-of-flight mass spectrometry (UHPLC-QTOF-MSE) and nuclear magnetic resonance (NMR) spectroscopy. Chemometric analyses such as principal component analysis (PCA) aided in understanding metabolite distribution. Biological assays demonstrated cytotoxic activities of specific fractions against cancer cell lines, notably the PhyN 4n fraction from P. niruri, which induced S-phase cell cycle arrest and apoptosis in HL60 cells. These findings underscore the anticancer potential of Phyllanthus species and lay the groundwork for future drug development efforts. The study's integration of advanced analytical techniques, chemometrics, and biological assays provides valuable insights for harnessing natural products in the fight against cancer.

Prevalence of oral manifestations of leprosy: a systematic review and meta-analysis.

OBJECTIVE: To evaluate the prevalence of oral manifestations of leprosy. STUDY DESIGN: This systematic review with meta-analysis was reported according to the Preferred Reporting Items for Systematic Reviews and Meta-Analyses guidelines and included cross-sectional studies by 2 independent reviewers in 2 phases, who reported bacilloscopic-confirmed oral manifestations of leprosy. Studies were selected based on predetermined eligibility criteria. Searches in 6 main databases were performed, such as PubMed/MEDLINE, Embase, Scopus, Web of Science, LILACS/BVS, and LIVIVO, in addition to the gray literature. The risk of bias was assessed using the JBI Checklist for Analytical Cross-Sectional Studies, and the quantitative synthesis of the data was performed using the Jamovi 2.3 application. RESULTS: Ten studies were included, and the meta-analysis was performed with 917 patients with different types of leprosy. The overall prevalence of oral alterations was 6.0% (95% IC, 0.02-0.11; I2 = 97.01%; Q = 75.56), with plaques (27.2%), infiltrations (18.1%), and macules (15.1%) being the most prevalent fundamental lesions, especially in multibacillary patients. CONCLUSIONS: Even though there is no oral pathognomonic lesion of leprosy, this infectious disease can manifest in oral tissues in different ways, depending mainly on the leprosy type and stage of treatment.

Bovine serum albumin nanoparticles containing Poly (I:C) can enhance the neutralizing antibody response induced by envelope protein of Orthoflavivirus zikaense.

Since the Orthoflavivirus zikaense (ZIKV) has been considered a risk for Zika congenital syndrome development, developing a safe and effective vaccine has become a high priority. Numerous research groups have developed strategies to prevent ZIKV infection and have identified the domain III of the ZIKV envelope protein (zEDIII) as a promising target. Subunit antigens are often poorly immunogenic, necessitating the use of adjuvants and/or delivery systems to induce optimal immune responses. The subject of nanotechnology has substantial expansion in recent years in terms of research and applications. Nanoparticles could be used as drug delivery systems and to increase the immunogenicity and stability of a given antigen. This work aims to characterize and validate the potential of a vaccine formulation composed of domain zEDIII and bovine serum albumin nanoparticles containing polyinosinic-polycytidylic acid (NPPI). NPPI were uptake in vitro by immature bone marrow dendritic cells and histological analysis of the skin of mice treated with NPPI showed an increase in cellularity. Immunization assay showed that mice immunized with zEDIII in the presence of NPPI produced neutralizing antibodies. Through the passive transfer of sera from immunized mice to ZIKV-infected neonatal mice, it was demonstrated that these antibodies provide protection, mitigating weight loss, clinical or neurological signs induced by infection, and significantly increased survival rates. Protection was further substantiated by the reduction in the number of viable infectious ZIKV, as well as a decrease in inflammatory cytokines and tissue alterations in the brains of infected mice. Taken together, data presented in this study shows that NPPI + zEDIII is a promising vaccine candidate for ZIKV.

Gravidade dos sintomas da covid-19 e qualidade de vida na covid longa: qual a relação em adultos e idosos? / Severity of covid-19 symptoms and quality of life in long covid: what is the relationship between adults and the elderly? / Gravedad de los síntomas de covid-19 y calidad de vida en long covid: ¿cuál es la relación entre adultos y ancianos?

Avaliar a relação entre gravidade dos sintomas da Covid-19 e qualidade de vida na Covid Longa em adultos e idosos. Trata-se de estudo transversal, prospectivo e multicêntrico, realizado com indivíduos com idade superior á 18 anos com Covid Longa, de ambos os sexos, acompanhados nas Clínicas Escolas da Universidade Paulista ­ UNIP. Foi aplicado uma ficha de avaliação, no qual foram coletadas informações como, dados pessoais, história clínica da Covid-19, e manejos da doença em relação a necessidade de oxigenoterapia, ventilação mecânica não invasiva (VNI) e invasiva (VM) e traqueostomia (TQT), bem como o questionário SF36 versão Brasileira. A pesquisa foi submetida e aprovada pelo CEP da Universidade Paulista ­ UNIP, parecer 4.894.652. Os adultos e idosos que foram internados tiveram pior qualidade de vida nos domínios capacidade funcional, vitalidade e saúde mental. Aqueles que utilizaram oxigenoterapia, VM e TQT, tiveram maior acometimento no domínio saúde mental e os indivíduos que utilizaram a VNI, tiveram pior pontuação no domínio capacidade funcional. Conclui-se que adultos e idosos com Covid Longa apresentaram pior qualidade de vida à longo prazo, sobretudo naqueles com maior gravidade dos sintomas da Covid-19, ou seja, que necessitaram de internação, oxigenoterapia, VNI, VM e traqueostomia.

To evaluate the relationship between the severity of Covid-19 symptoms and quality of life adults and elderly with Long Covid in. This is a cross-sectional, prospective and multicenter study, carried out with individuals aged over 18 years with Long Covid, of both gender, followed at the Clinic School of Universidade Paulista - UNIP. Infor-mations such as personal data, clinical history of Covid-19, and disease management in relation to used of oxygen therapy, non-invasive (NIV) and invasive mechanical ventila-tion and tracheostomy was collected. SF36 questionnaire version Brazilian was aplied to assess quality of life . Adults and elderly people who were hospitalized had a worse quality of life in functional capacity, vitality and mental health domains. Furthermore, those who used oxygen therapy, intubation and tracheostomy, had worse mental health. The individuals who used NIV had worse scores in domain capacity works. It is con-cluded that adults and elderly people with Long Covid had a worse quality of life in the long term, especially in those with more severe symptoms of Covid-19, that is, those who required hospitalization, oxygen therapy, invasive and non-invasive mechanical ventilation and tracheostomy.

Relacionar la gravedad de los síntomas de Covid-19 y la calidad de vida en adultos y ancianos con Largo Covid. Métodos: Se trata de un estudio transversal, prospectivo y multicéntrico, realizado con individuos mayores de 18 años con Largo Covid, de ambos sexos, seguidos en las Clínicas Escolas de la Universidade Paulista - UNIP. Se aplicó un formulario de evaluación, en el cual se recolectó información como datos personales, antecedentes clínicos de Covid-19 y manejo de la enfermedad en relación a la necesidad de oxigenoterapia, ventilación mecánica no invasiva e invasiva y traqueotomía, así como la Cuestionario SF36 versión brasileña. Resultados: Los adultos y ancianos hospitalizados tenían peor calidad de vida en los dominios capacidad funcional, vitalidad y salud mental. De los cuales usaban oxigenoterapia, intubación y traqueotomía, tenían peor salud mental. Los individuos que usaban ventilación mecánica no invasiva tenían peores puntuaciones en el domínio del la capacidad funcional. Conclusión: Se concluye que los adultos y ancianos con Covid Largo tuvieron una peor calidad de vida a largo plazo, especialmente en aquellos con síntomas más severos de Covid-19, es decir, aquellos que requirieron hospitalización, oxigenoterapia, invasiva y no invasiva. ventilación mecánica y traqueotomía.

Exclusive breastfeeding intention among pregnant women and associated variables: a cross-sectional study in a Brazilian community / Intenção de amamentar entre gestantes e variáveis associadas: estudo transversal em uma comunidade brasileira

ABSTRACT Objective: To investigate exclusive breastfeeding (EBF) intention and associated variables among women in the third trimester of pregnancy. Methods: The data were collected with a questionnaire for the pregnant women (n=653), from December/2018 to November/2019. They answered the Infant Feeding Intentions (IFI) scale, translated and adapted to Brazilian Portuguese, and a questionnaire on sociodemographic, biological, family, pregnancy, breastfeeding, health care, and habits variables. Descriptive statistics and multiple logistic regression analyses were performed with a 5% significance level, following a multilevel hierarchical model that estimated the association between the dependent and independent variables. The outcome EBF intention measured by the IFI score was dichotomized by the median (<16 or =16). Results: Mean±standard deviation score for the IFI scale was 14.4±2.6 (score 0: very strong intention to not breastfeed at all; score 16: very strong EBF intention up to six months). The results from the regression analysis showed that pregnant women who had no intention to bottle feed (OR=4.33; 95%CI 2.79-6.72) or did not know (OR=1.85; 95%CI 1.21-2.82), those who planned the pregnancy (OR=1.52; 95%CI 1.09-2.12), those who believed they would have help to care for the baby (OR=3.60; 95%CI 1.51-8.56) or did not know (OR=3.97; 95%CI 1.26-12.51), and those who reported knowing the World Health Organization recommendations on breastfeeding (OR=1.73; 95%CI 1.13-2.64) were more likely to show a very strong EBF intention. Conclusions: Pregnant women in the third trimester of pregnancy presented a strong EBF intention. The higher EBF intention score was significantly associated with the structural, setting, and individual determinants.

RESUMO Objetivo: Investigar a intenção materna de amamentar exclusivamente (IMA) e variáveis associadas entre as mulheres no terceiro trimestre de gravidez. Métodos: A coleta de dados foi realizada por meio de entrevista e questionário semiestruturado e autoadministrado, entre dezembro/2018 e novembro/2019. As gestantes (n=653) responderam à escala Infant Feeding Intentions (IFI) testada, traduzida e adaptada para o português do Brasil e ao questionário contendo perguntas sobre variáveis sociodemográficas, biológicas e familiares, bem como relacionadas à gestação, ao aleitamento materno, à assistência à saúde e a hábitos. Foram realizadas análises estatísticas descritivas e de regressão logística múltipla hierarquizada, com nível de significância de 5%, para estimar a associação entre as variáveis independentes e o desfecho. A IMA, medida pela escala IFI, foi dicotomizada pela mediana (<16 ou =16). Resultados: A pontuação média±desvio padrão para a escala IFI foi de 14,4±2,6. Os resultados da análise de regressão mostraram que as gestantes que não tinham intenção de oferecer mamadeira (OR=4,33; IC95% 2,79-6,72) ou não sabiam (OR=1,85; IC95% 1,21-2,82), que planejaram a gestação (OR=1,52; IC95% 1,09-2,12), aquelas que acreditavam que teriam ajuda nos cuidados com o bebê (OR=3,60; IC95% 1,51-8,56) ou que não sabiam (OR=3,97; IC95% 1,26-12,51), bem como aquelas que relataram conhecer as recomendações da Organização Mundial de Saúde sobre amamentação (OR=1,73; IC95% 1.13-2.64) tinham mais chances de mostrar uma IMA muito forte. Conclusões: As gestantes no terceiro trimestre de gestação apresentaram forte IMA. A maior IMA esteve significativamente associada aos determinantes estruturais, contextuais e individuais.

Third stage of the kangaroo method: exclusive breastfeeding and growth of preterm and/or low birth weight newborns / Terceira etapa do método canguru: aleitamento materno exclusivo e crescimento de recém-nascidos pré-termo e/ou de baixo peso ao nascer

Objective: To evaluate the rates of exclusive breastfeeding (EBF) and growth of preterm and/or low birth weight newborns during the third stage of the Kangaroo Method (TSKM), at discharge. Methods: Retrospective study in a reference public maternity hospital between Jan/2014 and Dec/2017, including the preterm (less than 37 weeks) and/or low birth weight (less than 2500 g) newborn infants. Information was collected from medical records. Statistics analysis was done in SPSS software. Results: 482 infants were included and followed up at the TSKM ambulatory. The average gestational age was 33 weeks (variation: 24-39 weeks) and birth weight, 1715g (variation: 455-2830 g). EBF occurred in 336 (70.1%) infants at hospital discharge, and in 291 (60.4%) at TSKM discharge. Each additional day of hospital stay increased the chance of infant formula (IF) use by 9.3% at hospital discharge and by 10.3% at TSKM discharge. Staying in the Kangaroo Neonatal Intermediate Care Unit (KNICU) favored EBF at hospital discharge and TSKM discharge (p<0.001). Not performing the kangaroo position increased the chance formula administration to the newborn infant at hospital discharge by 11%. Weight gain and head circumference growth were higher in infants using formula (p<0.001). Conclusions: The length of hospital stay and not performing the kangaroo position favored the use of infant formula at hospital and TSKM discharge. Staying in the KNICU favored exclusive breastfeeding at hospital and TSKM discharge. Weight gain and HC growth were higher in newborns receiving infant formula.

RESUMO Objetivo: Avaliar as taxas de aleitamento materno exclusivo e o crescimento dos recém-nascidos (RN) pré-termo e/ou de baixo peso ao nascer à alta da terceira etapa do Método Canguru (TEMC). Métodos: Estudo retrospectivo realizado em maternidade pública de referência entre janeiro/2014 e dezembro/2017, que incluiu os RN pré-termo (<37 semanas) e/ou de baixo peso (<2.500 g). As informações foram coletadas de prontuários médicos e analisadas pelo programa Statistical Package for the Social Sciences (SPSS). Resultados: Foram seguidos 482 RN, com mediana da idade gestacional de 33 semanas (variação: 24-39 sem) e peso ao nascer 1.715 g (variação: 455-2.830 g). O aleitamento materno exclusivo (AME) ocorreu em 336 (70,1%) RN à alta hospitalar e em 291 (60,4%) à alta da TEMC. Cada dia a mais de permanência hospitalar aumentou a chance do uso de fórmula infantil em 9,3% à alta hospitalar e em 10,3% à alta da TEMC. Permanecer na Unidade de Cuidados Intermediários Canguru (UCINCA) favoreceu o AME à alta hospitalar e da TEMC (p<0,001). Não realizar a Posição Canguru aumentou em 11% a chance de o RN utilizar fórmula infantil à alta hospitalar. O ganho de peso e crescimento do perímetro cefálico foram maiores nos RN em uso de fórmula infantil (p<0,001). Conclusões: O tempo de permanência hospitalar e a não realização da Posição Canguru favoreceram o uso de fórmula infantil à alta hospitalar e da TEMC. Permanecer na UCINCA favoreceu o AME à alta hospitalar e da TEMC. O ganho de peso e crescimento do PC foram maiores nos RN em uso de fórmula infantil.

Cytotoxic effect of crotoxin on cancer cells and its antitumoral effects correlated to tumor microenvironment: A review.

Cancer is the second leading cause of death worldwide, and despite the effort of standard treatments, the search for new tools against this disease is necessary. Importantly, it is known that the tumor microenvironment plays a crucial role in tumor initiation, progression, and response to therapies. Therefore, studies of potential drugs that act on these components are as critical as studies regarding antiproliferative substances. Through the years, studies of several natural products, including animal toxins, have been conducted to guide the development of medical compounds. In this review, we present the remarkable antitumor activities of crotoxin, a toxin from the rattlesnake Crotalus durissus terrificus, highlighting its effects on cancer cells and in the modulation of relevant elements in the tumor microenvironment as well as the clinical trials conducted with this compound. In summary, crotoxin acts through several mechanisms of action, such as activation of apoptosis, induction of cell cycle arrest, inhibition of metastasis, and decrease of tumor growth, in different tumor types. Crotoxin also modulates tumor-associated fibroblasts, endothelial cells, and immune cells, which contribute to its antitumoral effects. In addition, preliminary clinical studies confirm the promising results of crotoxin and support its potential future use as an anticancer drug.

Association between Pacifier Use, Bottle-Feeding, and Pre-Postnatal Variables: A Cohort Study

ABSTRACT Objective: To determine the prevalence and analyze the variables associated with the use of pacifiers and/or bottles by infants up to 6 months of age. Material and Methods: Data on sociodemographic characteristics, intention to offer pacifier and bottle-feeding, pregnancy and breastfeeding (BF) variables were collected at baseline by interviews and a self-administered questionnaire among pregnant women in the 3rd trimester. After delivery, mother-baby binomials were followed by phone calls at the 1st, 3rd, and 6th months of the baby's life (n=467) to gather information on the type of delivery, baby's gender, BF in the first hour of the newborn's life, baby's weight, mother's return to work, and oral habits. Association analyses were performed using logistic regression models with a 5% significance level with the pacifier/bottle-feeding use as the outcome. Results: Most mothers (52.5%) reported their babies used bottle-feeding, 48.2% used pacifiers and 33.4% used both of them throughout the 6 months. Intention to offer pacifier and bottle-feeding was reported by 45.0% and 54.8% of the mothers at the 3rd trimester of the pregnancy, respectively. Not living in one's own residence (OR=1.53; 95%CI: 1.05-2.24) and having the prenatal intention of offering a pacifier (OR=2.50; 95%CI: 1.63-3.83) to the baby were significantly associated with pacifier use. Variables significantly associated with bottle-feeding were mother's return to work (OR=2.48; 95%CI: 1.54-3.97), baby's lower birth weight (OR=1.58; IC95%: 1.07-2.33), and prenatal intention to offer bottle-feeding (OR=2.51; 95%CI 1.56-4.04). Conclusion: About half of the babies used pacifiers or were bottle-fed, which were associated with the mother's prenatal intention to offer them to their babies and socioeconomic factors.

Cross-cultural adaptation of the Breastfeeding Self-Efficacy Scale Short Form (BSES-SF) modified for preterm mothers in Brazil / Adaptação transcultural da Breastfeeding Self-Efficacy Scale Short Form (BSES-SF) modificada para mães de prematuros no Brasil / Adaptación transcultural de la Breastfeeding Self-Efficacy Scale Short Form (BSES-SF) modificada para madres de prematuras en Brasil

ABSTRACT Objectives: to conduct a cross-cultural adaptation of the Breastfeeding Self-Efficacy Scale-Short Form (BSES-SF) for mothers of ill and/or preterm infants among Portuguese-speaking mothers in Brazil. Methods: a methodological study was completed, including the translation of the tool, synthesis of translations, review by experts, synthesis, reassessment of experts, back-translation, pre-test, and validation of the content. The study involved 19 participants, including a translator and experts. In addition, 18 mothers from the target population were included in the pre-test. Results: the equivalences of the opinion obtained by the committee of experts were semantic (85%), idiom (89%), cultural (86%), and conceptual (94%). The content validation coefficient (CVC) on the scale was 0.93 for clarity and understanding; 0.89 for practical relevance; 0.92 for relevance; and the average overall CVC was 0.91. Conclusions: the scale was translated and adapted to the Brazilian Portuguese language, which maintained the equivalences and confirmed the content validity.

RESUMO Objetivos: realizar adaptação transcultural da Breastfeeding Self-Efficacy Scale-Short Form (BSES-SF) para mães de bebês doentes e/ou prematuros para a língua portuguesa no contexto brasileiro. Métodos: foi realizado um estudo metodológico, incluindo a tradução do instrumento, síntese das traduções, revisão por especialistas, síntese, reavaliação de especialistas, retrotradução, pré-teste e validação do conteúdo. O estudo envolveu 19 participantes, incluindo os tradutores e especialistas. Além disso, 18 mães da população-alvo foram incluídas no pré-teste. Resultados: as equivalências da opinião obtida pelo comitê de especialistas foram: semântica (85%), idiomática (89%), cultural (86%) e conceitual (94%). O coeficiente de validação de conteúdo (CVC) da escala foi de 0,93 para clareza e compreensão; 0,89 para relevância prática; 0,92 para relevância; e a média geral do CVC foi de 0,91. Conclusões: a escala foi traduzida e adaptada para a língua portuguesa do Brasil, o que manteve as equivalências e confirmou a validade de conteúdo.

RESUMEN Objetivos: realizar la adaptación transcultural de la Breastfeeding Self-Efficacy Scale-Short Form (BSES-SF) para madres de niños enfermos y/o prematuros al portugués en el contexto brasileño. Métodos: se realizó un estudio metodológico que incluyó la traducción del instrumento, síntesis de traducciones, revisión por especialistas, síntesis, reevaluación por especialistas, retrotraducción, preprueba y validación de contenido. El estudio involucró a 19 participantes, incluidos traductores y expertos. Además, 18 madres de la población objetivo fueron incluidas en la preprueba. Resultados: las equivalencias de opinión obtenidas por el comité de expertos fueron: semántica (85%), idiomática (89%), cultural (86%) y conceptual (94%). El Coeficiente de Validación de Contenido (CVC) de la escala fue de 0,93 para claridad y comprensión; 0,89 para relevancia práctica; 0,92 para relevancia. El promedio general del CVC fue de 0,91. Conclusiones: la escala fue traducida y adaptada al portugués brasileño, lo que mantuvo las equivalencias y confirmó la validez de contenido.

Inovação em cânula de lipoaspiração para coleta de enxerto de gordura para uso em reconstrução mamária / Innovation in liposuction cannula to harvest fat graft to use in breast reconstruction

Introdução: A evolução da técnica de lipoaspiração e da enxertia de gordura associada a segurança oncológica permitiram utilizar a gordura como preenchedor autólogo em pacientes com neoplasia de mama que eram submetidas a reconstrução mamária. O objetivo é apresentar uma inovação no instrumento de coleta de gordura que será utilizada como enxerto, para uso em reconstrução mamária. Métodos: Foi realizada a busca de anterioridade nos bancos de dados internacionais e nacional e a confecção de um protótipo de cânula de coleta de gordura de 35cm de comprimento, com diâmetro do tubo de 4mm, com 10 furos redondos na sua extremidade distal com 2mm de diâmetro. Foi realizado um experimento de aspiração de substância viscosa comparando o protótipo com dois modelos de cânulas com desenho padrão da indústria, de três furos tipo Mercedes e de cinco furos tipo Pitanguy. Resultados: Foram encontrados quatro patentes de alta relevância e uma patente de média relevância, que diferem do modelo de utilidade proposto quando se comparam o tipo de desenho da extremidade distal das cânulas estudadas e a função dos instrumentos identificados. Quando comparou-se a eficiência do protótipo, a cânula de cinco furos foi a mais eficiente na aspiração da substância viscosa, e não houve diferença estatística na velocidade de aspiração entre o protótipo e a cânula de três furos tipo Mercedes. Conclusão: A inovação apresentada para a coleta de enxerto de gordura para uso em reconstrução mamária apresentou a mesma eficiência que a cânula Mercedes de três furos neste modelo experimental.

Introduction: The evolution of liposuction and fat graft technique with the oncologic safety allowed to use the fat as autologous filler in patients with breast cancer and submitted to breast reconstruction. The objective is to introduce an innovation in the instrument used to harvest fat grafts for breast reconstruction. Methods: Search of anteriority and trademarks were performed at international and national databases, and a prototype was built as a fat harvest instrument with 35cm in length, 4cm in diameter, with 10 holes at the distal extremity, with 2mm diameter each. It was experimented with a harvest of a viscous substance, comparing the prototype with two regular industry cannulas types: three holes Mercedes' type and five roles Pitanguy's type. Results: Four high-relevance patents and one medium-relevance patent were found, which differ from the proposed utility model when comparing the type of design of the distal end of the studied cannulas and the function of the instruments identified. When the prototype's efficiency was compared with the other cannulas, the cannula with five holes in Pitanguy's type was the most efficient, and it was no statistical difference between the prototype and the cannula with three roles in Mercedes' type. Conclusion: The innovation presented to harvest fat graft for breast reconstruction had the same efficiency in harvesting the viscose substance as the cannula Mercedes type with three holes in this experimental model.

Análise dos efeitos da marcha suspensa na atividade eletromiográfica dos membros inferiores e irradiação motora aos membros superiores em lesados medulares / Analysis of the effects of body weight-supported gait training on the electromyographic activity of the lower limbs and motor irradiation to the upper limbs in spinal cord injured patients

A reabilitação na esteira com suporte parcial de peso (ESPP) nos indivíduos lesados medulares pode reproduzir de modo passivo a marcha, proporcionando-lhes a descarga de peso em membros inferiores (MMII) e a manutenção da postura ortostática. O objetivo do estudo foi analisar se, durante a marcha passiva na ESPP, existe irradiação motora de MMII aos membros superiores. Foram selecionados três indivíduos do gênero masculino, com diagnóstico de Traumatismo Raquimedular baixo (T10 e L1), classificados como ASIA A com preservação parcial de raízes nervosas. Os indivíduos foram submetidos a uma avaliação passiva da marcha na ESSP, utilizando a avaliação eletromiográfica dos músculos: bíceps braquial (BB), reto femoral (RF) e gastrocnêmio lateral (GL) bilateralmente e a uma análise estatística, incluindo testes de Shapiro-Wilk e de Levene, One way ANOVA, post-hoc Tukey HSD. Na condição clínica de repouso, observou-se menor ativação dos músculos BB bilateralmente, quando comparado aos músculos RF e GL, fato justificado pelos comprometimentos secundários advindos da lesão dos indivíduos. As condições clínicas do ciclo da marcha (CM) repetiram-se três vezes; no CM1 observou-se uma maior ativação eletromiográfica do músculo GL direito e uma menor ativação do músculo BB direito, e estatisticamente, GL direito e BB esquerdo demonstraram maior atividade média (resultados estatisticamente significantes). No CM2 o mesmo padrão de ativação do CM1 foi observado, no entanto o músculo RF direito reduziu seu limiar de ativação. No CM3 os níveis de ativação das condições anteriores mantiveram-se, no entanto, o músculo RF esquerdo apresentou aumento nos limiares de ativação eletromiográfica, quando comparado aos demais músculos. Com base nestes resultados, o músculo BB apresentou variações quantitativas na ativação eletromiográfica, determinando a presença de irradiação motora de MMII para superiores durante o CM. Em uma análise qualitativa, foi observado que durante as fases de apoio da marcha houve "picos" de ativação deste músculo. [au]

Rehabilitation on the treadmill with partial body weight support (PBWS) in individuals with spinal cord injuries can passively reproduce gait, helping them with weight bearing on the lower limbs (LL) and orthostatic posture. The objective of the study was to assess motor irradiation from the lower limbs to the upper limbs during gait training with PBWS. The participants were three male individuals diagnosed with low spinal cord injury (T10 and L1), classified as ASIA A, with partial preservation of nerve roots. Subjects were submitted to gait training with PBWS and electromyographic assessment of the muscles Biceps brachii (BB), Rectus femoris (RF) and Lateral Gastrocnemius (LG) on both sides. Statistical analysis included Shapiro-Wilk and de Levene tests, One way ANOVA test and Tukey's HSD post-hoc test. In the resting state, there was less activity of BB muscles on both sides when compared to the RF and LG, which can be explained by the secondary impairments arising from the individuals' injury. The gait cycle (GC) was repeated three times; in GC1 there was greater electromyographic activity in the right LG muscle and lower activity of the right BB muscle and, statistically, right LG and left BB showed higher mean activity (statistically significant results). In GC2, the same pattern of activity of GC1 was observed, but the right RF muscle reduced its activation threshold. In GC3, the activity levels of the previous cycles were maintained, but the left RF muscle showed an increase in the thresholds of electromyographic activation when compared to the other muscles. Based on these results, the BB muscle presented quantitative variations in electromyographic activation, demonstrating the presence of motor irradiation from LL to upper limbs during the GC. The qualitative analysis showed "peaks" of activity in this muscle mainly during the support phases of the gait training. [au]

Effects of topiramate, bupropion and naltrexone isolated or combined on subcutaneous adipose tissue in obese rats.

OBJECTIVE: To evaluate the effects of combining topiramate, bupropion and naltrexone in obesity-induced rats on their weight and subcutaneous adipose tissue. METHODS: A total of 40 male Wistar rats were induced to obesity for 8 weeks and the animals were divided into 8 groups: Ctr - control, G0 - Sham, G1 - oral saline solution (1.0mL/day), G2 - topiramate (20.0mg/kg) and bupropion (5.0mg/kg), G3 - naltrexone (20.0mg/kg), G4 - topiramate (20.0mg/kg), G5 - bupropion (5.0mg/kg) and G6 - topiramate (20.0mg/kg), bupropion (5.0mg/kg) and naltrexone (20.0mg/kg). During the experiment, all animals were weighed weekly. After 30 days of treatment animals were euthanized and their skin fragments were processed and stained with hematoxylin and eosin for morphological, morphometric and biochemical analyzes. RESULTS: The only group that presented a decrease in the volume of subcutaneous adipose tissue was G3, but this decrease was not significant when compared with the other groups. The G4, the G5 and the G6 presented increased adipose tissue volume. Data showed that until the eighth week all animals increased their weight by approximately 50%. After treatment animals of all groups, except G3, increased their weight from 4% to 9% approximately. The G3 was the only group that lost weight, but this decrease was not significant. CONCLUSION: The medicines studied were not efficient in reducing weight in obese rats. However, it should be considered that 30-day treatment period is not enough to observe the stronger effects of these drugs.

Espectro clínico da constipação intestinal funcional e características do hábito intestinal de escolares recrutados em duas escolas e em um ambulatório especializado / Clinical spectrum of functional constipation and bowel-habit patterns of schoolchildren recruited from two elementary schools and a specialized outpatient clinic

ABSTRACT Background: Early diagnosis of functional constipation is important for reducing its negative consequences on the health of children and adolescents. Objective: To describe the clinical spectrum of functional constipation and bowel habit patterns in schoolchildren recruited from two primary schools and patients from a pediatric gastroenterology outpatient clinic. Methods: This cross-sectional study included 452 students from two elementary schools in the city of Osasco and 81 patients with functional constipation seen in an outpatient clinic specializing in pediatric gastroenterology. All children were aged between 6 and 12 years. The Rome IV criteria (two features for more than 1 month) and the Bristol scale were used. Results: The prevalence of functional constipation among the elementary school students was 22.3% (n=101). Among the 351 students who did not have functional constipation, 182 (51.9%) had one of the clinical manifestations of the Rome IV criteria. Bristol stool scale types 1 and 2 were observed in 14 (8.3%) of the 169 students without any clinical manifestation of the Rome IV criteria and in 28 (15.4%) of the 182 students who presented one of the Rome IV criteria for functional constipation (P=0.060). A comparison of the clinical manifestations of children with functional constipation identified at school in relation to those seen at the specialized clinic showed the following differences: fewer than two bowel movements per week (21.8% and 54.3%; P<0.001, respectively), one or more episodes of fecal incontinence per week (14.8% and 53.1%; P<0.001), and retentive posturing (70.3% and 40.7%, P<0.001). Only 18 (17.8%) of the 101 students identified at the schools with functional constipation had received any treatment for this disease in the previous 2 months. Conclusion: As expected, the frequency of more severe clinical manifestations was higher in children seen at specialized clinics. Only a small proportion of the children identified with functional constipation at primary schools had undergone any form of treatment in the previous 2 months. More than half of the children without functional constipation in elementary schools reported one of the Rome IV clinical manifestations. Finally, functional constipation has a broad clinical spectrum and also requires attention for the prevention and the management of its early clinical manifestations.

RESUMO Contexto: Diagnóstico precoce da constipação intestinal funcional é importante para reduzir suas consequências negativas para a saúde da criança e do adolescente. Objetivo: Descrever o espectro clínico da constipação intestinal funcional e o hábito intestinal de crianças recrutadas em escola de primeiro grau e de pacientes atendidos em ambulatório especializado de gastroenterologia pediátrica. Métodos: Estudo observacional que avaliou 452 alunos de duas escolas públicas da cidade de Osasco e 81 pacientes atendidos em ambulatório especializado em gastroenterologia pediátrica com constipação intestinal funcional. Todas as crianças tinham idade entre 6 e 12 anos. Foram utilizados os critérios de Roma IV (duas manifestações clínicas por mais de 1 mês) e a escala de Bristol. Resultados: Na escola constatou-se que 22,3% (101) das crianças apresentavam constipação intestinal funcional. Dentre os 351 alunos que não apresentavam constipação intestinal funcional, verificou-se que 182 (51,9%) apresentavam uma das manifestações clínicas do critério de Roma IV. A comparação das características clínicas das crianças com constipação intestinal funcional identificadas na escola (n=101) em relação aos pacientes atendidos no ambulatório especializado (n=81) evidenciou, respectivamente, as seguintes diferenças: menos de duas evacuações por semana (21,8% e 54,3%; P<0,001); um ou mais episódios de incontinência fecal por semana (14,8% e 53,1%; P<0,001) e comportamento de retenção (70,3% e 40,7%, P<0,001). Apenas 18 (17,8%) dos 101 alunos identificados na escola com constipação intestinal funcional havia realizado algum tratamento para esta doença nos últimos dois meses. Conclusão Conforme esperado, a frequência de manifestações clínicas mais graves foi maior nas crianças atendidas no ambulatório de gastroenterologia pediátrica. Apenas uma pequena parcela das crianças com constipação intestinal funcional identificadas nas escolas recebeu algum tratamento nos últimos dois meses. Mais da metade das crianças sem constipação intestinal funcional da escola apresentava pelo menos uma das manifestações do critério de Roma IV. Para finalizar, constipação intestinal funcional apresenta um amplo espectro clínico que também requer atenção para a sua prevenção e controle de suas manifestações clínicas precoces.

"Cuida!": práticas de cuidado em saúde com mulheres trabalhadoras do sexo / "Take care!" "care!": health care practices with sex workers / "¡Cuida!": prácticas de atención médica con trabajadores sexuales

O objetivo deste trabalho é relatar a experiência do Projeto de extensão universitária, "Cuida!", desenvolvido pelo Núcleo de Estudos sobre Drogas do Ceará e parceiros. O "Cuida!" envolve ações de cuidado em saúde direcionadas a trabalhadoras sexuais, com foco na Redução de Danos e na integralidade da atenção em saúde. Dentre as ações desenvolvidas estão:1) Testagem rápida de HIV/IST; 2) Consultas com profissionais da saúde; 3) Orientações sobre o acesso a políticas públicas; 4) Rodas de conversas e oficinas com diferentes temáticas. O "Cuida!" teve como resultado a ampliação do vínculo e do diálogo das mulheres com as equipes dos equipamentos, além do fortalecimento das estratégias de Redução de danos que passaram a ser referência de cuidado no território. Apostando em conhecer a vida que re-existe, toda proposta de cuidado foi assentada em um projeto ético-político, potencializando assim os processos de experimentação e resistência produzido pelas e com as mulheres. (AU)

The objective ofthis work is to report the experience of the University Extension Project, "Cuida!", Developed by the Center for Drug Studies in Ceará and partners. "Cuida!" it involves health care actions directed at sex workers, with a focus on Harm Reduction and comprehensive health care. Among the actions developed are: 1) Rapid HIV /STI testing; 2) Consultations with health professionals; 3) Guidelines on access to public policies; 4) Conversation circles and workshops with different themes. "Cuida!" the result was the expansion of the bond and dialogue of women with the equipment teams, in addition to strengthening the harm reduction strategies that have become a reference for care in the territory. Betting on knowing the life that re-exists, every care proposal was based on an ethical-political project, thus enhancing the processes of experimentation and resistance produced by and with women. (AU)

El objetivo de este trabajoes dar a conocer la experiencia del Proyecto de Extensión Universitaria "¡Cuida!", Desarrollado por el Centro de Estudios sobre Drogas de Ceará y socios. "¡Cuídate!" se trata de acciones de atención de la salud dirigidas a las trabajadoras sexuales, con un enfoque en la Reducción de Daños y la atención integral de la salud. Entre las acciones desarrolladas se encuentran: 1) Prueba rápida de SIDA/IST; 2) Consultas con profesionales de la salud; 3) Lineamientos sobre acceso a políticas públicas; 4) Círculos de conversación y talleres con diferentes temáticas. "¡Cuídate!" el resultado fue la ampliación del vínculo y el diálogo de las mujeres con los equipos de equipos, además de fortalecer las estrategias de reducción de daños que se han convertido en un referente de atención en el territorio. Apostando por conocer la vida que reexiste, toda propuesta de cuidado se basó en un proyecto ético-político, potenciando así los procesos de experimentación y resistencia producidos por y con las mujeres. (AU)

Perfuração de luvas cirúrgicas: limpeza de produtos para saúde versus procedimentos cirúrgicos / Surgical glove perforation: cleaning of medical devices versus surgical procedures / Perforación de guantes quirúrgicos: limpieza de productos sanitarios versus procedimientos quirúrgicos

Objetivo: Comparar os índices de perfuração de luvas cirúrgicas utilizadas em cirurgias gerais e na limpeza manual de produtos para a saúde. Método: Estudo desenvolvido num hospital de ensino de grande porte em Goiânia, Goiás. Foram comparados os índices de perfuração de luvas cirúrgicas obtidos por meio de teste de vazamento em dois grupos de luvas: (I) as utilizadas durante a limpeza manual de produtos para a saúde e (II) as utilizadas em cirurgias gerais. Resultados: A frequência de perfuração foi superior nas luvas utilizadas para a limpeza de produtos para saúde (46,1%) do que naquelas empregadas em cirurgias gerais (12,5%) (p = 0,047), e o tempo de uso interferiu no aumento do risco de perfuração em ambos os grupos. Não houve diferença nas perfurações das mãos direita e esquerda nem no uso de luvas simples e sobrepostas. Conclusão: O índice de perfuração de luvas cirúrgicas durante a limpeza manual revela maior risco ao trabalhador que realiza essa atividade e reforça a contraindicação desse tipo de luva para tal finalidade.

Objective: To compare the surgical glove perforation rates in general surgeries and in the manual cleaning of medical devices. Method: This study was developed in a large teaching hospital in Goiânia, Goiás, Brazil. We compared the surgical glove perforation rates obtained from the leak test performed in two groups of gloves: (I) those used during manual cleaning of medical devices and (ii) those used in general surgeries. Results: The frequency of perforation was higher in the gloves used for cleaning medical devices (46.1%) than in those used for general surgeries (12.5%) (p=0.047), and the duration of use was associated with increased risk of perforation in both groups. No difference was found regarding perforations in the right and left hands or in the use of single or double gloving. Conclusion: The surgical glove perforation rate during manual cleaning reveals a higher risk to the worker who performs this activity and reinforces the contraindication of this type of glove for this purpose.

Objetivo: Comparar las tasas de perforación de los guantes quirúrgicos utilizados en cirugías generales y en la limpieza manual de productos sanitarios. Método: Estudio desarrollado en un gran hospital de enseñanza en Goiânia, Goiás. Se compararon las tasas de perforación de guantes quirúrgicos, obtenidas mediante prueba de fugas, en dos grupos: (I) utilizados durante la limpieza manual de productos sanitarios y (II) utilizados en cirugía general. Resultados: La frecuencia de perforación fue mayor en los guantes utilizados para limpieza de productos sanitarios (46,1%) que en los utilizados para cirugía general (12,5%) (p = 0,047) y el tiempo de uso interfirió en el aumento del riesgo de perforación en ambos grupos. No hubo diferencia en las perforaciones de la mano derecha e izquierda y el uso de guantes simples y superpuestos. Conclusión: La tasa de perforación de los guantes quirúrgicos durante la limpieza manual revela el mayor riesgo para el trabajador que realiza esta actividad y refuerza la contraindicación de este tipo de guantes para este fin.

Effects of topiramate, bupropion and naltrexone isolated or combined on subcutaneous adipose tissue in obese rats

ABSTRACT Objective: To evaluate the effects of combining topiramate, bupropion and naltrexone in obesity-induced rats on their weight and subcutaneous adipose tissue. Methods: A total of 40 male Wistar rats were induced to obesity for 8 weeks and the animals were divided into 8 groups: Ctr - control, G0 - Sham, G1 - oral saline solution (1.0mL/day), G2 - topiramate (20.0mg/kg) and bupropion (5.0mg/kg), G3 - naltrexone (20.0mg/kg), G4 - topiramate (20.0mg/kg), G5 - bupropion (5.0mg/kg) and G6 - topiramate (20.0mg/kg), bupropion (5.0mg/kg) and naltrexone (20.0mg/kg). During the experiment, all animals were weighed weekly. After 30 days of treatment animals were euthanized and their skin fragments were processed and stained with hematoxylin and eosin for morphological, morphometric and biochemical analyzes. Results: The only group that presented a decrease in the volume of subcutaneous adipose tissue was G3, but this decrease was not significant when compared with the other groups. The G4, the G5 and the G6 presented increased adipose tissue volume. Data showed that until the eighth week all animals increased their weight by approximately 50%. After treatment animals of all groups, except G3, increased their weight from 4% to 9% approximately. The G3 was the only group that lost weight, but this decrease was not significant. Conclusion: The medicines studied were not efficient in reducing weight in obese rats. However, it should be considered that 30-day treatment period is not enough to observe the stronger effects of these drugs.

Intersetorialidade e políticas sociais no contexto brasileiro: desafios para a proteção integral infantojuvenil

Resumo: O artigo em questão tem como objetivo discutir sobre como tem se efetivado a intersetorialidade no atendimento à crianças e adolescentes em situação de violência intrafamiliar no Brasil, tendo como recorte um município na região noroeste do Paraná. Por meio de uma pesquisa de caráter documental, analisamos as atas das reuniões da rede de serviços públicos do município em pauta ocorridas entre os anos de 2013 a 2017. As atas revelaram que apesar da roupagem intersetorial, as ações na rede se dão de modo fragmentado por meio da divisão de tarefas ou competências, responsabilizando ainda, alguns serviços específicos pelo êxito ou não dos encaminhamentos previamente dados nas reuniões. Por ora, vemos que as práticas intersetoriais tem se efetivado, via políticas sociais, distantes daquilo que é proposto e que apesar das contradições estruturais da lógica dos direitos sociais, o desenvolvimento de um trabalho que cumpra o previsto pelas diretrizes, princípios e objetivos dessas políticas deve ser o foco do Estado.

Abstract: The present article aim to discuss about how intersectoriality has been implemented in the care of children and adolescents in situations of interfamily violence in Brazil, focused in a municipality in the northwest of Parana. Through a documental research, we analyzed the minutes of the municipality's public service network meetings that occurred between 2013 and 2017. The minutes showed that despite the intersectoral outlook, the network actions are fragmented through the division of tasks or skills, also holding responsible some specific services for the success or not of the referrals previously given in the meetings. For now, we see that intersectoral practices has been effected, through social policies, distant from what is proposed by them, and, despite the structural contradictions of the social rights logic, the development of a work that achieves what is predicted by guidelines, principles and objectives of these policies must be the focus of the State.

Resumen: El articulo presentado tiene como objetivo plantear una discusión sobre como se ha efectivado la intersectorialidad hacia la asistencia a niños, niñas y adolescentes en situación de violencia intrafamiliar en Brasil, centrándose en una ciudad del noroeste del estado de Paraná. Bajo una investigación documental, analizamos las actas de las reuniones de la red de servicios públicos que se desarrollaron entre los años 2013 hasta 2017. El análisis de las actas ha demostrado que, aunque exista una perspectiva intersectorial, las acciones en la red se desarrollan de una manera fragmentada por medio de la división de tareas o competencias y se otorgan determinados servicios específicos según el éxito, o no, de las referencias dadas previamente en las reuniones. Mientras tanto, vemos que se han implementado prácticas intersectoriales, por medio de políticas sociales, lejanas de lo que se propone. Reconocemos que - aunque haya contradicciones estructurales en la lógica de los derechos sociales - el desarrollo de un trabajo que cumpla con los lineamientos, principios y objetivos de estas políticas debe ser el foco del Estado.

Retrospective study of poxviruses diagnosed in cattle from Goiás State, Brazil (2010-2018) / Estudo retrospectivo de poxviroses diagnosticadas em bovinos no estado de Goiás (2010-2018)

A retrospective study of poxvirus infections diagnosed in cattle from Goiás state (GO), Brazil, from 2010 to 2018, was performed. All cases have been investigated by the GO Official Veterinary Service (Agrodefesa), from which technical forms and protocols of veterinary diagnosis laboratories were reviewed. In most cases, samples of oral or cutaneous tissues and/or swabs were submitted for virological diagnosis by polymerase chain reaction (PCR) and/or virus isolation. Thirty seven outbreaks/cases of vesicular disease were notified in cattle of 25 counties; in 33 cases the animals presented lesions clinically compatible with poxviruses. The etiology of 25 out of 33 outbreaks/cases was confirmed as poxviruses by PCR and/or viral isolation: 13 as bovine vaccinia virus (VACV), six as pseudocowpox virus (PCPV), five as bovine papular stomatitis virus (BPSV) and one coinfection (VACV and an Orf virus-like parapoxvirus). The laboratory confirmed that cases occurred mainly in dairy cattle (19/25) and during the dry season (22/25). In adult cattle, gross changes were observed mainly in the teats and udder and included vesicles, ulcers, crusts, papules and scars and varied of type, severity and affected region, depending on the poxvirus species. In calves, the main lesions were ulcers in the mouth and muzzle. Zoonotic lesions compatible with poxvirus infections were observed for all diagnosed poxviruses, affecting especially the hands of milkers and other farm workers. Our data demonstrate the sanitary and economic relevance of these diseases and the wide circulation of different poxviruses in cattle from GO.(AU)

Foi realizado um estudo retrospectivo das infecções por poxvírus diagnosticadas em bovinos do estado de Goiás (GO), entre 2010 e 2018. Todos os casos foram investigados pela Agência Goiana de Defesa Agropecuária (Agrodefesa). Foram revisados formulários técnicos e protocolos de laboratórios de diagnóstico veterinário. Na maioria dos casos, amostras de tecidos orais ou cutâneos e/ou swabs foram encaminhadas para diagnóstico virológico. Foram notificados 37 surtos/casos de doença vesicular em bovinos em 25 municípios; em 33 casos os animais apresentavam lesões clinicamente compatíveis com poxvírus. A etiologia de 25 de 33 surtos/casos foi confirmada como poxvírus por PCR e/ou isolamento viral: 13 como vírus vaccínia (VACV), seis como vírus pseudocowpox (PCPV), cinco como vírus da estomatite papular bovina (BPSV) e um caso de coinfecção (VACV e um parapoxvírus semelhante ao Orf vírus). Os casos confirmados laboratorialmente ocorreram principalmente em bovinos leiteiros (19/25) e durante a estação seca (22/25). Em bovinos adultos, alterações macroscópicas foram observadas principalmente nas tetas e úbere e incluíram vesículas, úlceras, crostas, pápulas e cicatrizes e variaram quanto ao tipo, gravidade e região afetada, dependendo da espécie do poxvírus. Em bezerros, as principais lesões foram úlceras na boca e focinho. Lesões zoonóticas compatíveis com infecção por poxvírus foram observadas em todas as poxviroses diagnosticadas, afetando principalmente as mãos dos ordenhadores e outros trabalhadores rurais. Nossos dados demonstram a relevância sanitária e econômica dessas doenças e a ampla circulação de diferentes poxvírus em bovinos de GO.(AU)