RESUMO
OBJECTIVE: Glutathione S-transferases (GSTs) are important enzymes that are involved in detoxification of environmental carcinogens. Molecular epidemiological studies have been conducted to investigate the association between GSTM1 and GSTT1 homozygous deletion polymorphisms and brain tumours but results have been conflicting. The aim of this study was to clarify this problem using a meta-analysis. METHODS: A total of 9 records were identified by searching the PubMed and Embase databases. Fixed- and random-effects models were performed to estimate the pooled odds ratios. RESULTS: No significant association was found between the GSTM1 and GSTT1 homozygous deletion polymorphisms and risk of brain tumours, including glioma and meningioma. Similar negative results were also observed in both population-based and hospital-based studies. CONCLUSION: These findings indicate that the GSTM1 and GSTT1 polymorphisms may not be related to the development of brain tumours.
Assuntos
Neoplasias Encefálicas/genética , Glioma/genética , Glutationa Transferase/genética , Neoplasias Meníngeas/genética , Polimorfismo Genético/genética , Neoplasias Encefálicas/epidemiologia , Estudos de Casos e Controles , China/epidemiologia , Predisposição Genética para Doença , Glioma/epidemiologia , Homozigoto , Humanos , Neoplasias Meníngeas/epidemiologia , Metanálise como Assunto , Prognóstico , Fatores de RiscoRESUMO
OBJECTIVE: To establish the craniopharyngioma cell line with primary culture which might provide experiment background and evidence for future eternal tumor cell line establishment. METHODS: Thirty six surgical specimens were collected from patients with craniopharyngiomas definited by iconography and pathology examinations in West China Hospital, Sichuan University, including twenty one adamantine epitheliomas and fifteen squamous papillary tumors. The tumor cell was treated through primary cukture, purification, passage, freezing, resuscitation, and identified by keratin 7 staining through SP method. The growth curve and double time were detected through trypan blue dye cell count and MTT assay. The growth of the tumor cells treated with growth hormone (GH) and Tamoxifen was also observed. RESULTS: Thirty six primary cultures were done, 29 of which were successful and subculture was achieved in 80.6% of all primary cultures. These cell lines were from squamous epithelium by keratin 7 antibody identification, with three days of double time. Proloferative effect of GH was most prevalent at 100 ng/mL, while tamoxifen suppressed cell growth. CONCLUSION: The finite craniopharyngioma cell lines were obtained through primary culture.