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1.
Nat Commun ; 13(1): 3729, 2022 06 28.
Artigo em Inglês | MEDLINE | ID: mdl-35764640

RESUMO

The repeated, rapid and often pronounced patterns of evolutionary divergence observed in insular plants, or the 'plant island syndrome', include changes in leaf phenotypes, growth, as well as the acquisition of a perennial lifestyle. Here, we sequence and describe the genome of the critically endangered, Galápagos-endemic species Scalesia atractyloides Arnot., obtaining a chromosome-resolved, 3.2-Gbp assembly containing 43,093 candidate gene models. Using a combination of fossil transposable elements, k-mer spectra analyses and orthologue assignment, we identify the two ancestral genomes, and date their divergence and the polyploidization event, concluding that the ancestor of all extant Scalesia species was an allotetraploid. There are a comparable number of genes and transposable elements across the two subgenomes, and while their synteny has been mostly conserved, we find multiple inversions that may have facilitated adaptation. We identify clear signatures of selection across genes associated with vascular development, growth, adaptation to salinity and flowering time, thus finding compelling evidence for a genomic basis of the island syndrome in one of Darwin's giant daisies.


Assuntos
Elementos de DNA Transponíveis , Genômica , Evolução Biológica , Elementos de DNA Transponíveis/genética , Sintenia/genética
2.
Eur J Pharmacol ; 891: 173685, 2021 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-33127363

RESUMO

α7 nicotinic acetylcholine receptor (α7 nAChR) is an extensively validated target for several neurological and psychiatric conditions namely, dementia and schizophrenia, owing to its vital roles in cognition and sensorimotor gating. Positive allosteric modulation (PAM) of α7 nAChR represents an innovative approach to amplify endogenous cholinergic signaling in a temporally restricted manner in learning and memory centers of brain. α7 nAChR PAMs are anticipated to side-step burgeoning issues observed with several clinical-stage orthosteric α7 nAChR agonists, related to selectivity, tolerance/tachyphylaxis, thus providing a novel dimension in therapeutic strategy and pharmacology of α7 nAChR ion-channel. Here we describe a novel α7 nAChR PAM, LL-00066471, which potently amplified agonist-induced Ca2+ fluxes in neuronal IMR-32 neuroblastoma cells in a α-bungarotoxin (α-BTX) sensitive manner. LL-00066471 showed excellent oral bioavailability across species (mouse, rat and dog), low clearance and good brain penetration (B/P ratio > 1). In vivo, LL-00066471 robustly attenuated cognitive deficits in both procognitive and antiamnesic paradigms of short-term episodic and recognition memory in novel object recognition task (NORT) and social recognition task (SRT), respectively. Additionally, LL-00066471 mitigated apomorphine-induced sensorimotor gating deficits in acoustic startle reflex (ASR) and enhanced antipsychotic efficacy of olanzapine in conditioned avoidance response (CAR) task. Further, LL-00066471 corrected redox-imbalances and reduced cortico-striatal infarcts in stroke model. These finding together suggest that LL-00066471 has potential to symptomatically alleviate cognitive deficits associated with dementias, attenuate sensorimotor gating deficits in schizophrenia and correct redox-imbalances in cerebrovascular disorders. Therefore, LL-00066471 presents potential for management of cognitive impairments associated with neurological and psychiatric conditions.


Assuntos
Comportamento Animal/efeitos dos fármacos , Encéfalo/efeitos dos fármacos , Colinérgicos/farmacologia , Cognição/efeitos dos fármacos , Disfunção Cognitiva/prevenção & controle , Transtornos Neurológicos da Marcha/prevenção & controle , Filtro Sensorial/efeitos dos fármacos , Receptor Nicotínico de Acetilcolina alfa7/efeitos dos fármacos , Animais , Encéfalo/metabolismo , Encéfalo/fisiopatologia , Linhagem Celular Tumoral , Colinérgicos/farmacocinética , Disfunção Cognitiva/metabolismo , Disfunção Cognitiva/fisiopatologia , Disfunção Cognitiva/psicologia , Modelos Animais de Doenças , Cães , Comportamento Exploratório/efeitos dos fármacos , Transtornos Neurológicos da Marcha/metabolismo , Transtornos Neurológicos da Marcha/fisiopatologia , Transtornos Neurológicos da Marcha/psicologia , AVC Isquêmico/tratamento farmacológico , AVC Isquêmico/metabolismo , AVC Isquêmico/fisiopatologia , Masculino , Camundongos Endogâmicos BALB C , Teste de Campo Aberto/efeitos dos fármacos , Estresse Oxidativo/efeitos dos fármacos , Ratos Sprague-Dawley , Ratos Wistar , Reflexo de Sobressalto/efeitos dos fármacos , Transdução de Sinais , Comportamento Social , Receptor Nicotínico de Acetilcolina alfa7/metabolismo
3.
Bioorg Med Chem ; 28(24): 115819, 2020 12 15.
Artigo em Inglês | MEDLINE | ID: mdl-33120078

RESUMO

The exploitation of GLU988 and LYS903 residues in PARP1 as targets to design isoquinolinone (I & II) and naphthyridinone (III) analogues is described. Compounds of structure I have good biochemical and cellular potency but suffered from inferior PK. Constraining the linear propylene linker of structure I into a cyclopentene ring (II) offered improved PK parameters, while maintaining potency for PARP1. Finally, to avoid potential issues that may arise from the presence of an anilinic moiety, the nitrogen substituent on the isoquinolinone ring was incorporated as part of the bicyclic ring. This afforded a naphthyridinone scaffold, as shown in structure III. Further optimization of naphthyridinone series led to identification of a novel and highly potent PARP1 inhibitor 34, which was further characterized as preclinical candidate molecule. Compound 34 is orally bioavailable and displayed favorable pharmacokinetic (PK) properties. Compound 34 demonstrated remarkable antitumor efficacy both as a single-agent as well as in combination with chemotherapeutic agents in the BRCA1 mutant MDA-MB-436 breast cancer xenograft model. Additionally, compound 34 also potentiated the effect of agents such as temozolomide in breast cancer, pancreatic cancer and Ewing's sarcoma models.


Assuntos
Antineoplásicos/química , Naftiridinas/química , Poli(ADP-Ribose) Polimerase-1/antagonistas & inibidores , Inibidores de Poli(ADP-Ribose) Polimerases/química , Quinolonas/química , Animais , Antineoplásicos/metabolismo , Antineoplásicos/farmacologia , Antineoplásicos/uso terapêutico , Sítios de Ligação , Linhagem Celular Tumoral , Sobrevivência Celular/efeitos dos fármacos , Meia-Vida , Humanos , Camundongos , Camundongos Endogâmicos BALB C , Simulação de Acoplamento Molecular , Naftiridinas/metabolismo , Neoplasias/tratamento farmacológico , Neoplasias/patologia , Poli(ADP-Ribose) Polimerase-1/metabolismo , Inibidores de Poli(ADP-Ribose) Polimerases/metabolismo , Inibidores de Poli(ADP-Ribose) Polimerases/farmacologia , Inibidores de Poli(ADP-Ribose) Polimerases/uso terapêutico , Quinolonas/metabolismo , Relação Estrutura-Atividade , Transplante Heterólogo
4.
Plant Physiol ; 166(3): 1186-99, 2014 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-24399359

RESUMO

Parasitic flowering plants are one of the most destructive agricultural pests and have major impact on crop yields throughout the world. Being dependent on finding a host plant for growth, parasitic plants penetrate their host using specialized organs called haustoria. Haustoria establish vascular connections with the host, which enable the parasite to steal nutrients and water. The underlying molecular and developmental basis of parasitism by plants is largely unknown. In order to investigate the process of parasitism, RNAs from different stages (i.e. seed, seedling, vegetative strand, prehaustoria, haustoria, and flower) were used to de novo assemble and annotate the transcriptome of the obligate plant stem parasite dodder (Cuscuta pentagona). The assembled transcriptome was used to dissect transcriptional dynamics during dodder development and parasitism and identified key gene categories involved in the process of plant parasitism. Host plant infection is accompanied by increased expression of parasite genes underlying transport and transporter categories, response to stress and stimuli, as well as genes encoding enzymes involved in cell wall modifications. By contrast, expression of photosynthetic genes is decreased in the dodder infective stages compared with normal stem. In addition, genes relating to biosynthesis, transport, and response of phytohormones, such as auxin, gibberellins, and strigolactone, were differentially expressed in the dodder infective stages compared with stems and seedlings. This analysis sheds light on the transcriptional changes that accompany plant parasitism and will aid in identifying potential gene targets for use in controlling the infestation of crops by parasitic weeds.


Assuntos
Cuscuta/genética , Regulação da Expressão Gênica de Plantas , Nicotiana/parasitologia , Solanum lycopersicum/parasitologia , Transcriptoma , Sequência de Bases , Análise por Conglomerados , Cuscuta/fisiologia , Flores/parasitologia , Biblioteca Gênica , Sequenciamento de Nucleotídeos em Larga Escala , Interações Hospedeiro-Parasita , Anotação de Sequência Molecular , Dados de Sequência Molecular , Caules de Planta/parasitologia , Plantas Daninhas/genética , Plantas Daninhas/fisiologia , RNA de Plantas/química , RNA de Plantas/genética , Plântula/parasitologia , Sementes/parasitologia , Análise de Sequência de RNA
5.
Front Plant Sci ; 4: 121, 2013.
Artigo em Inglês | MEDLINE | ID: mdl-23653631

RESUMO

Plant secondary cell walls are deposited mostly in vascular tissues such as xylem vessels, tracheids, and fibers. These cell walls are composed of a complex matrix of compounds including cellulose, hemicellulose, and lignin. Lignin functions primarily to maintain the structural and mechanical integrity of both the transport vessel and the entire plant itself. Since lignin has been identified as a major source of biomass for biofuels, regulation of secondary cell wall biosynthesis has been a topic of much recent investigation. Biosynthesis and patterning of lignin involves many developmental and environmental cues including evolutionarily conserved transcriptional regulatory modules and hormonal signals. Here, we investigate the role of the class I Knotted1-like-homeobox (KNOX) genes and gibberellic acid in the lignin biosynthetic pathway in a representative monocot and a representative eudicot. Knotted1 overexpressing mutant plants showed a reduction in lignin content in both maize and tobacco. Expression of four key lignin biosynthesis genes was analyzed and revealed that KNOX1 genes regulate at least two steps in the lignin biosynthesis pathway. The negative regulation of lignin both in a monocot and a eudicot by the maize Kn1 gene suggests that lignin biosynthesis may be preserved across large phylogenetic distances. The evolutionary implications of regulation of lignification across divergent species are discussed.

6.
Proc Natl Acad Sci U S A ; 110(6): 2401-6, 2013 Feb 05.
Artigo em Inglês | MEDLINE | ID: mdl-23341595

RESUMO

Leaf morphology and the pattern of shoot branching determine to a large extent the growth habit of seed plants. Until recently, the developmental processes that led to the establishment of these morphological structures seemed unrelated. Here, we show that the tomato Trifoliate (Tf) gene plays a crucial role in both processes, affecting the formation of leaflets in the compound tomato leaf and the initiation of axillary meristems in the leaf axil. Tf encodes a myeloblastosis oncoprotein (MYB)-like transcription factor related to the Arabidopsis thaliana LATERAL ORGAN FUSION1 (LOF1) and LOF2 proteins. Tf is expressed in the leaf margin, where leaflets are formed, and in the leaf axil, where axillary meristems initiate. During tomato ontogeny, expression of Tf in young leaf primordia increases, correlating with a rise in leaf dissection (heteroblasty). Formation of leaflets and initiation of axillary meristems can be traced back to groups of pluripotent cells. Tf function is required to inhibit differentiation of these cells and thereby to maintain their morphogenetic competence, a fundamental process in plant development. KNOTTED1-LIKE proteins, which are known regulators in tomato leaf dissection, require Tf activity to exert their function in the basal part of the leaf. Similarly, the plant hormone auxin needs Tf activity to initiate the formation of lateral leaflets. Thus, leaf dissection and shoot branching rely on a conserved mechanism that regulates the morphogenetic competence of cells at the leaf margin and in the leaf axil.


Assuntos
Proteínas de Plantas/genética , Solanum lycopersicum/crescimento & desenvolvimento , Solanum lycopersicum/genética , Fatores de Transcrição/genética , Sequência de Aminoácidos , Sequência de Bases , DNA de Plantas/genética , Regulação da Expressão Gênica no Desenvolvimento , Regulação da Expressão Gênica de Plantas , Genes de Plantas , Proteínas de Homeodomínio/genética , Proteínas de Homeodomínio/metabolismo , Ácidos Indolacéticos/metabolismo , Solanum lycopersicum/anatomia & histologia , Solanum lycopersicum/metabolismo , Meristema/crescimento & desenvolvimento , Microscopia Eletrônica de Varredura , Dados de Sequência Molecular , Mutação , Filogenia , Reguladores de Crescimento de Plantas/metabolismo , Folhas de Planta/crescimento & desenvolvimento , Folhas de Planta/metabolismo , Proteínas de Plantas/metabolismo , Brotos de Planta/crescimento & desenvolvimento , Brotos de Planta/metabolismo , Plantas Geneticamente Modificadas , Homologia de Sequência de Aminoácidos , Fatores de Transcrição/metabolismo
7.
Plant Cell ; 24(7): 3153-66, 2012 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-22822208

RESUMO

Infection of crop species by parasitic plants is a major agricultural hindrance resulting in substantial crop losses worldwide. Parasitic plants establish vascular connections with the host plant via structures termed haustoria, which allow acquisition of water and nutrients, often to the detriment of the infected host. Despite the agricultural impact of parasitic plants, the molecular and developmental processes by which host/parasitic interactions are established are not well understood. Here, we examine the development and subsequent establishment of haustorial connections by the parasite dodder (Cuscuta pentagona) on tobacco (Nicotiana tabacum) plants. Formation of haustoria in dodder is accompanied by upregulation of dodder KNOTTED-like homeobox transcription factors, including SHOOT MERISTEMLESS-like (STM). We demonstrate interspecific silencing of a STM gene in dodder driven by a vascular-specific promoter in transgenic host plants and find that this silencing disrupts dodder growth. The reduced efficacy of dodder infection on STM RNA interference transgenics results from defects in haustorial connection, development, and establishment. Identification of transgene-specific small RNAs in the parasite, coupled with reduced parasite fecundity and increased growth of the infected host, demonstrates the efficacy of interspecific small RNA-mediated silencing of parasite genes. This technology has the potential to be an effective method of biological control of plant parasite infection.


Assuntos
Cuscuta/fisiologia , Nicotiana/parasitologia , Doenças das Plantas/parasitologia , Proteínas de Plantas/genética , Interferência de RNA/fisiologia , RNA Interferente Pequeno/fisiologia , Arabidopsis/genética , Cuscuta/citologia , Cuscuta/genética , Cuscuta/crescimento & desenvolvimento , Regulação para Baixo , Regulação da Expressão Gênica de Plantas , Sequenciamento de Nucleotídeos em Larga Escala , Proteínas de Homeodomínio/genética , Interações Hospedeiro-Parasita , Brotos de Planta/citologia , Brotos de Planta/genética , Brotos de Planta/parasitologia , Brotos de Planta/fisiologia , Feixe Vascular de Plantas/citologia , Feixe Vascular de Plantas/genética , Feixe Vascular de Plantas/parasitologia , Feixe Vascular de Plantas/fisiologia , Plantas Geneticamente Modificadas , Transporte de RNA , RNA Interferente Pequeno/genética , Nicotiana/citologia , Nicotiana/genética , Nicotiana/fisiologia
8.
Eur J Pharmacol ; 607(1-3): 213-9, 2009 Apr 01.
Artigo em Inglês | MEDLINE | ID: mdl-19239913

RESUMO

The present study, investigates the effect of RBx 6198, 2-{3-[4-(2-Isopropoxy-phenyl)-piperazin-1-yl]-propyl}-3a, 4, 7, 7a-tetrahydro-isoindole-1, 3,-dione, a novel alpha(1)-adrenoceptor antagonist, in both in vitro and in vivo test systems. RBx 6198 is a potent (nanomolar affinity) alpha(1A)-adrenoceptor antagonist with demonstrable uroselectivity in anaesthesized dog model. In radioligand binding studies using human recombinant receptors, RBx 6198 exhibited high selectivity (approximately 50 fold) for the alpha(1A)-adrenoceptor subtype as compared to alpha(1B)-adrenoceptor subtype. In order to assess tissue selectivity, the antagonistic effect of RBx 6198 on the phenylephrine induced contractile response of isolated rat prostate, spleen and aorta was characterized. RBx 6198 was 8 fold more potent in inhibiting phenylephrine-evoked contractions of isolated tissues compared to tamsulosin. However, the compound was non-selective for alpha(1A) vs. alpha(1D)-adrenoceptor like tamsulosin. In anaesthetized beagle dogs RBx 6198 suppressed the intraurethral pressure response to phenylephrine to a greater extent than the mean arterial pressure response thereby demonstrating uroselectivity consistent with in vitro binding and functional data. RBx 6198 was 6.4 fold more uroselective as compared to tamsulosin after i.v. route dose administration. Taken together all results from preclinical studies, it is suggested that RBx 6198 is a novel alpha(1)-adrenoceptor antagonist that exhibited improved pharmacological profile over tamsulosin in both in vitro and in vivo.


Assuntos
Antagonistas de Receptores Adrenérgicos alfa 1 , Antagonistas Adrenérgicos alfa/farmacologia , Ftalimidas/farmacologia , Piperazinas/farmacologia , Hiperplasia Prostática/tratamento farmacológico , Antagonistas Adrenérgicos alfa/administração & dosagem , Animais , Aorta/efeitos dos fármacos , Aorta/metabolismo , Células CHO , Cricetinae , Cricetulus , Cães , Humanos , Injeções Intravenosas , Masculino , Contração Muscular/efeitos dos fármacos , Fenilefrina , Ftalimidas/administração & dosagem , Piperazinas/administração & dosagem , Próstata/efeitos dos fármacos , Próstata/metabolismo , Hiperplasia Prostática/fisiopatologia , Ligação Proteica , Ensaio Radioligante , Ratos , Ratos Wistar , Baço/efeitos dos fármacos , Baço/metabolismo , Sulfonamidas/farmacologia , Tansulosina
9.
Cold Spring Harb Protoc ; 2009(10): pdb.emo133, 2009 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-20147034

RESUMO

The genus Kalanchoë plays an important role in the investigation of biochemical, physiological and phylogenetic aspects of Crassulacean acid metabolism (CAM) in plants, which is an important evolutionary adaptation of the photosynthetic carbon assimilation pathway to arid environments. In addition, natural compounds extracted from tissues of Kalanchoë have potential applicability in treating tumors and inflammatory and allergic diseases, and have been shown to have insecticidal properties. Kalanchoë daigremontiana (Hamet & Perrier) originated in Madagascar and reproduces asexually by spontaneously forming whole plantlets on leaves. Plantlets develop symmetrically along the leaf margins on leaf notches, closely resembling zygotic embryos in development, and once the root system is formed, they detach from the mother-leaf, fall to the ground, and grow into new plants. This phenomenon is also found in other species from this same genus; however, the formation of leaf-plantlets is variable among species. Nevertheless, all species illustrate the remarkable ability of plant somatic cells to regenerate an entire organism, which has fascinated the scientific community for many years. It was only recently that the morphogenic process involved in the origin of K. daigremontiana plantlets was determined using molecular and genetic tools: K. daigremontiana forms plantlets by co-opting both organogenesis and embryogenesis programs into leaves. The ability of K. daigremontiana species to form somatic embryos outside of a seed environment provides an attractive model system to study somatic embryogenesis in nature, particularly the molecular mechanism involved in the acquisition of competence by vegetative cells to make embryos without fertilization.


Assuntos
Kalanchoe/genética , Kalanchoe/fisiologia , Reprodução Assexuada , Ácidos/metabolismo , Botânica/métodos , Crassulaceae/metabolismo , Flores/metabolismo , Genes de Plantas , Modelos Biológicos , Fotossíntese/fisiologia , Folhas de Planta/metabolismo , Fenômenos Fisiológicos Vegetais , Proteínas de Plantas/genética , Sementes , Especificidade da Espécie
10.
CSH Protoc ; 2008: pdb.emo105, 2008 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-21356708

RESUMO

INTRODUCTIONTomato (Solanum lycopersicum) is one of the most important vegetable plants in the world. It originated in western South America, and domestication is thought to have occurred in Central America. Because of its importance as food, tomato has been bred to improve productivity, fruit quality, and resistance to biotic and abiotic stresses. Tomato has been widely used not only as food, but also as research material. The tomato plant has many interesting features such as fleshy fruit, a sympodial shoot, and compound leaves, which other model plants (e.g., rice and Arabidopsis) do not have. Most of these traits are agronomically important and cannot be studied using other model plant systems. There are 13 recognized wild tomato species that display a great variety of phenotypes and can be crossed with the cultivated tomato. These wild tomatoes are important for breeding, as sources of desirable traits, and for evolutionary studies. Current progress on the tomato genome sequencing project has generated useful information to help in the study of tomato. In addition, the tomato belongs to the extremely large family Solanaceae and is closely related to many commercially important plants such as potato, eggplant, peppers, tobacco, and petunias. Knowledge obtained from studies conducted on tomato can be easily applied to these plants, which makes tomato important research material. Because of these facts, tomato serves as a model organism for the family Solanaceae and, specifically, for fleshy-fruited plants.

11.
CSH Protoc ; 2008: pdb.prot5083, 2008 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-21356723

RESUMO

INTRODUCTIONGrafting is agronomically important because one can combine desirable aboveground characteristics (such as fruit size) and underground characteristics (such as resistance to soil-borne diseases). This protocol describes the simplest way of grafting tomato plants using "top wedge grafting" or "cleft grafting." Potatoes, eggplants, and tobacco plants are closely related to tomatoes, and they can be grafted onto each other as well. Although the grafting of vegetable crops is still rare, this technique has been useful in reducing infections caused by pathogens, increasing resistance to drought, and enhancing nutrient uptake.

12.
Development ; 130(18): 4405-15, 2003 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-12900456

RESUMO

Recent work on species with simple leaves suggests that the juxtaposition of abaxial (lower) and adaxial (upper) cell fates (dorsiventrality) in leaf primordia is necessary for lamina outgrowth. However, how leaf dorsiventral symmetry affects leaflet formation in species with compound leaves is largely unknown. In four non-allelic dorsiventrality-defective mutants in tomato, wiry, wiry3, wiry4 and wiry6, partial or complete loss of ab-adaxiality was observed in leaves as well as in lateral organs in the flower, and the number of leaflets in leaves was reduced significantly. Morphological analyses and expression patterns of molecular markers for ab-adaxiality [LePHANTASTICA (LePHAN) and LeYABBY B (LeYAB B)] indicated that ab-adaxial cell fates were altered in mutant leaves. Reduction in expression of both LeT6 (a tomato KNOX gene) and LePHAN during post-primordial leaf development was correlated with a reduction in leaflet formation in the wiry mutants. LePHAN expression in LeT6 overexpression mutants suggests that LeT6 is a negative regulator of LePHAN. KNOX expression is known to be correlated with leaflet formation and we show that LeT6 requires LePHAN activity to form leaflets. These phenotypes and gene expression patterns suggest that the abaxial and adaxial domains of leaf primordia are important for leaflet primordia formation, and thus also important for compound leaf development. Furthermore, the regulatory relationship between LePHAN and KNOX genes is different from that proposed for simple-leafed species. We propose that this change in the regulatory relationship between KNOX genes and LePHAN plays a role in compound leaf development and is an important feature that distinguishes simple leaves from compound leaves.


Assuntos
Proteínas de Homeodomínio/genética , Folhas de Planta/anatomia & histologia , Proteínas de Plantas/genética , Proteínas Proto-Oncogênicas c-myb/genética , Solanum lycopersicum/anatomia & histologia , Regulação da Expressão Gênica de Plantas , Genes de Plantas , Proteínas de Homeodomínio/metabolismo , Hibridização In Situ , Solanum lycopersicum/genética , Morfogênese/fisiologia , Mutação , Fenótipo , Epiderme Vegetal/citologia , Epiderme Vegetal/metabolismo , Folhas de Planta/fisiologia , Proteínas de Plantas/metabolismo , Proteínas Proto-Oncogênicas c-myb/metabolismo
13.
Nature ; 424(6947): 438-43, 2003 Jul 24.
Artigo em Inglês | MEDLINE | ID: mdl-12879073

RESUMO

Diverse leaf forms in nature can be categorized as simple or compound. Simple leaves, such as those of petunia, have a single unit of blade, whereas compound leaves, such as those of tomato, have several units of blades called leaflets. Compound leaves can be pinnate, with leaflets arranged in succession on a rachis, or palmate, with leaflets clustered together at the leaf tip. The mechanisms that generate these various leaf forms are largely unknown. The upper (adaxial) surface is usually different from the bottom (abaxial) surface in both simple and compound leaves. In species with simple leaves, the specification of adaxial and abaxial cells is important for formation of the leaf blade, and the MYB transcription factor gene PHANTASTICA (PHAN) is involved in maintaining the leaf adaxial (upper) domain. Here we show that downregulation of PHAN is sufficient to reduce the adaxial domain of leaf primordia and to change pinnate compound leaves into palmate compound leaves. Furthermore, this mechanism seems to be shared among compound leaves that arose independently.


Assuntos
Perfilação da Expressão Gênica , Regulação da Expressão Gênica de Plantas , Folhas de Planta/crescimento & desenvolvimento , Folhas de Planta/metabolismo , Proteínas de Plantas/genética , Proteínas de Plantas/metabolismo , Proteínas Proto-Oncogênicas c-myb/genética , Proteínas Proto-Oncogênicas c-myb/metabolismo , Regulação para Baixo , Genes de Plantas/genética , Dados de Sequência Molecular , Morfogênese , Especificidade de Órgãos , Filogenia , Caules de Planta/crescimento & desenvolvimento , Caules de Planta/metabolismo
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