[Influence of Different Type of Surfactant on Bacteriolytic Activity of Lysozyme].

The influence ofvarious surfactants (anionic sodium dodecyl sulfate, SDS, cationic dodecyltrimethylarnmonium bromide, DTAB, and zwitterionic cocoamidopropylbetaine, CAPB) on the activity of the chicken egg lysozyme is investigated. Lysis of Gram-positive bacteria by the enzyme was carried out at pH 7.2 and ionic strength of 0.15 M. It was found that at low SDS and DTAB concentrations (less than 1 x 10(-5) M) the bacteriolytic activity increases by 30-140%. At higher concentrations (1 x 10(-5) - 1 x 10(4) M) the activity returns to the level observed in the absence of the surfactants. The elevated activity correlated with the formation of hydrophobic lysozyme-surfactant complexes. Introduction of CAPB at concentrations above 1 x 10(-5) M sig, nificantly diminished the bacteriolytic activity due to CAPB induced aggregation of lysozyme.

[Ways of improving of intensive care for victims with severe concomitant injury].

In terms of prognosis of the outcome of traumatic disease as a disease of adaptation particularly significant is a functional component. Updating of options of the course of traumatic disease allow to combine two principles of intensive care such as individual focus and standardization. Innovations of recent years, embedded into the battlefield surgery clinic of Military Medical Academy n. a. S.M.Kirov in tactics and content of intensive care, improved the current situation on the results of treatment of patients with polytrauma.