Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 2 de 2
Filtrar
Mais filtros











Base de dados
Intervalo de ano de publicação
1.
Protein Pept Lett ; 18(9): 858-62, 2011 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-21443499

RESUMO

CTX-M-15, an extended-spectrum ß-lactamase emerging worldwide, hydrolyzes lactam ring of ß-lactam antibiotics, and thus causes therapeutic failure and a lack of eradication of pathogenic bacteria by third-generation ß-lactams. Therefore, the enzyme is a potential target for developing agents against pathogens isolated from patients suffering from nosocomial infections. The CTX-M-15 protein was purified and crystallized at 298 K. X-ray diffraction data from CTX-M-15 crystal have been collected to 1.46 Å resolution using synchrotron radiation. The crystal of CTX-M-15 belongs to space group P2(1)2(1)2(1), with unit-cell parameters a = 45.50, b = 44.23, and c = 116.92 Å. Analysis of the packing density shows that the asymmetric unit probably contains two molecules with a solvent content of 41.26%.


Assuntos
Infecções por Escherichia coli/enzimologia , Escherichia coli/enzimologia , beta-Lactamases/química , Cristalização , Cristalografia por Raios X , Escherichia coli/química , Escherichia coli/isolamento & purificação , Infecções por Escherichia coli/tratamento farmacológico , Humanos , Resistência beta-Lactâmica , beta-Lactamases/isolamento & purificação
2.
Antimicrob Agents Chemother ; 49(4): 1600-3, 2005 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-15793150

RESUMO

The outcome of Helicobacter pylori infection was analyzed in 114 dyspeptic patients treated with triple-drug therapy including clarithromycin. Clarithromycin resistance (in 20.2% of our isolates) was mainly caused by an A2142G mutation in the 23S rRNA gene of H. pylori. H. pylori eradication was obtained in all patients with clarithromycin-susceptible isolates but not in any patients with clarithromycin-resistant isolates (P = 0.0001). Therefore, it would be useful to conduct H. pylori antimicrobial susceptibility testing of the first gastric biopsy culture before choosing the first three drugs for therapy of infected patients.


Assuntos
Antibacterianos/farmacologia , Claritromicina/farmacologia , Farmacorresistência Bacteriana , Infecções por Helicobacter/tratamento farmacológico , Helicobacter pylori/efeitos dos fármacos , Adulto , Farmacorresistência Bacteriana/genética , Feminino , Genes de RNAr , Infecções por Helicobacter/microbiologia , Helicobacter pylori/genética , Humanos , Masculino , Testes de Sensibilidade Microbiana , Pessoa de Meia-Idade , Mutação , Úlcera Péptica/microbiologia , RNA Ribossômico 23S/genética , Resultado do Tratamento
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA