RESUMO
Ethnopharmacological relevance of Saussurea species for anti-cancer compounds instigated us to develop chemotherapeutic herbal tablets. This study was an ongoing part of our previous research based on the scientific evaluation of Saussurea heteromalla (S. heteromalla) for anti-cancer lead compounds. In the current study, S. heteromalla herbal tablets (500 /800 mg) were designed and evaluated for anti-cancer activity. Arctigenin was found as a bioactive lead molecule with anti-cancer potential for cervical cancer. The in vitro results on the HeLa cell line supported the ethnopharmacological relevance and traditional utilization of S. heteromalla and provided the scientific basis for the management of cervical cancer as proclaimed by traditional practitioners in China. LD50 of the crude extract was established trough oral acute toxicity profiling in mice, wherein the minimum lethal dose was noticed as higher than 1000 mg/kg body weight orally. Chromatographic fingerprint analysis ensured the identity and consistency of S. heteromalla in herbal tablets in terms of standardization of the herbal drug. About 99.15% of the drug (S. heteromalla crude extract) was recovered in herbal tablets (RSD: 0.45%). In vitro drug release profile was found to be more than 87% within 1 h, which was also correlated with different mathematical kinetic models of drug release (r2 = 0.992), indicating that drug release from matrix tablets into the blood is constant throughout the delivery. The dosage form was found stable after an accelerated stability parameters study which may be used for anti-cervical cancer therapy in the future, if it qualifies successful preclinical investigation parameters.
Assuntos
Extratos Vegetais , Saussurea , Saussurea/química , Animais , Humanos , Camundongos , Células HeLa , Extratos Vegetais/química , Extratos Vegetais/toxicidade , Extratos Vegetais/farmacologia , Lignanas/farmacologia , Lignanas/química , Feminino , Furanos/toxicidade , Furanos/química , Furanos/farmacologia , Comprimidos , Antineoplásicos Fitogênicos/farmacologia , Antineoplásicos Fitogênicos/química , Masculino , Antineoplásicos/farmacologia , Antineoplásicos/química , Dose Letal Mediana , Testes de Toxicidade Aguda , Medicamentos de Ervas Chinesas/química , Medicamentos de Ervas Chinesas/toxicidade , Medicamentos de Ervas Chinesas/farmacologiaRESUMO
Vapor-triggered crystal-to-crystal transformation between a discrete trinuclear complex [Ni3(sih)2(py)8] and a two-dimensional (2D) coordination polymer [Ni3(sih)2(py)2]n·2DMF·2H2O was demonstrated. It provides an example of a solid-state coordination-induced spin state switch behavior attributed to the structural phase transition triggered by solvent signal. The reversible nature can be detected by both optical (spectral) and magnetic responses in cycles.