Assuntos
Doença de Alzheimer/genética , Carcinoma de Células Escamosas/genética , Caspase 3/genética , Caspase 3/metabolismo , Proteínas Nucleares/genética , Proteínas Nucleares/metabolismo , Couro Cabeludo , Neoplasias Cutâneas/genética , Proteínas Supressoras de Tumor/genética , Proteínas Supressoras de Tumor/metabolismo , Idoso de 80 Anos ou mais , Doença de Alzheimer/patologia , Apoptose/genética , Carcinoma de Células Escamosas/patologia , Expressão Gênica , Regulação Neoplásica da Expressão Gênica , Humanos , Leucócitos/metabolismo , Masculino , RNA Mensageiro/metabolismo , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Neoplasias Cutâneas/patologiaAssuntos
Carcinoma de Células Escamosas/genética , Caspase 3/fisiologia , Expressão Gênica/fisiologia , Neoplasias de Cabeça e Pescoço/genética , Doença de Parkinson/genética , Poli(ADP-Ribose) Polimerases/fisiologia , Apoptose/genética , Carcinoma de Células Escamosas/complicações , Neoplasias de Cabeça e Pescoço/complicações , Humanos , Doença de Parkinson/complicações , Poli(ADP-Ribose) Polimerase-1 , Reação em Cadeia da Polimerase em Tempo RealRESUMO
INTRODUCTION: Head and neck squamous cell carcinoma (HNSCC) is one of the most common cancers in the world. Risk factors for this cancer include tobacco and alcohol use, ultraviolet light exposure, and viral infection. Parkinson's disease is one of the most common neurodegenerative disorders, with a prevalence of 3% in persons over the age of 65 years. Apoptosis is a programmed cell death machinery pivotal for normal development, the establishment of highly organized neuronal circuitry, and the elimination of cancer cells. It has been suggested that increased expression of proapoptotic genes is associated with head tumors. One of these genes is the leucine zipper, down-regulated in cancer 1 (LDOC1) gene. CASE REPORT: We report two interesting cases of a 79-year-old man and a 98-year-old woman, both with Parkinson's disease and well-differentiated multiple HNSCC, in whom we evaluated the possible differential expression of LDOC1. RESULTS: We found that LDOC1 gene expression was increased in both patients compared with three male and three female controls. Conclusions. These findings suggest that apoptosis may play a pathogenetic role in HNSCC.
Assuntos
Apoptose , Carcinoma Basocelular/química , Carcinoma de Células Escamosas/química , Neoplasias de Cabeça e Pescoço/química , Leucócitos/química , Proteínas Nucleares/análise , Doença de Parkinson/complicações , Proteínas Supressoras de Tumor/análise , Idoso , Idoso de 80 Anos ou mais , Antiparkinsonianos/uso terapêutico , Carcinoma Basocelular/sangue , Carcinoma Basocelular/complicações , Carcinoma Basocelular/patologia , Carcinoma de Células Escamosas/sangue , Carcinoma de Células Escamosas/complicações , Carcinoma de Células Escamosas/patologia , Eletroforese em Gel de Ágar , Neoplasias Faciais/química , Feminino , Regulação Neoplásica da Expressão Gênica , Gliceraldeído-3-Fosfato Desidrogenase (Fosforiladora)/metabolismo , Neoplasias de Cabeça e Pescoço/sangue , Neoplasias de Cabeça e Pescoço/complicações , Neoplasias de Cabeça e Pescoço/patologia , Humanos , Zíper de Leucina , Levodopa/uso terapêutico , Masculino , Proteínas Nucleares/genética , Doença de Parkinson/tratamento farmacológico , RNA Mensageiro/análise , Reação em Cadeia da Polimerase em Tempo Real , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Espectrofotometria , Neoplasias da Língua/química , Neoplasias da Língua/metabolismo , Proteínas Supressoras de Tumor/genéticaRESUMO
We report the case of a 74-year-old man with Alzheimer's disease (AD) and an extensive ulcerative lesion on the right ear. AD is a neurodegenerative disease with progressive loss of memory and cognitive deterioration. It has been suggested that apoptotic cell injury and eventually cell death is a major contributor to the AD neurodegenerative process. The ulcerative lesion was surgically excised and the histological analysis reported a well-differentiated squamous cell carcinoma. Caspase-3 (CASP3) plays an important role in neuronal death during nervous system development and under certain pathological conditions. Furthermore, in vitro and in vivo studies reported elevated expression and activation of CASP3 in models of AD. Molecular epidemiological studies suggest that CASP3 may contribute to head and neck squamous cell carcinoma susceptibility and disease progression and that increased CASP3 expression is associated with tumors of the head. Also poly (ADP-ribose) polymerase 1 (PARP1) and the leucine zipper downregulated in cancer 1 (LDOC1) genes play a proapoptotic role. We therefore evaluated the differential expression of LDOC1, PARP1, and CASP3 mRNA in peripheral blood leukocytes of our patient. We found increased expression of all these genes compared with the expression in control subjects.
Assuntos
Doença de Alzheimer/genética , Carcinoma de Células Escamosas/genética , Caspase 3/genética , Proteínas Nucleares/genética , Poli(ADP-Ribose) Polimerases/genética , Neoplasias Cutâneas/genética , Proteínas Supressoras de Tumor/genética , Idoso , Doença de Alzheimer/complicações , Apoptose/genética , Carcinoma de Células Escamosas/complicações , Caspase 3/biossíntese , Regulação Neoplásica da Expressão Gênica , Humanos , Leucócitos/metabolismo , Masculino , Proteínas Nucleares/biossíntese , Poli(ADP-Ribose) Polimerase-1 , Poli(ADP-Ribose) Polimerases/biossíntese , RNA Mensageiro/biossíntese , Neoplasias Cutâneas/complicações , Proteínas Supressoras de Tumor/biossínteseAssuntos
Apoptose/genética , Melanoma/genética , Melanoma/secundário , Proteínas Nucleares/genética , Poli(ADP-Ribose) Polimerases/genética , Neoplasias Cutâneas/genética , Neoplasias Cutâneas/patologia , Proteínas Supressoras de Tumor/genética , Regulação para Baixo , Regulação Neoplásica da Expressão Gênica , Humanos , Metástase Linfática , Masculino , Pessoa de Meia-Idade , Poli(ADP-Ribose) Polimerase-1 , RNA Mensageiro/análiseRESUMO
The incidence of melanoma has dramatically increased in many countries (it is 4.5 cases every 100 000 inhabitants in Sicily) and Xq27 region contains genes important in cancer like the SPANX (sperm protein associated with the nucleus in the X chromosome) gene family. These genes, made up of two exons separated by an intron of about 650 base pair, are expressed in sperm cells and in many tumours, including melanoma. These observations suggested that SPANX genes, or some of them, may be involved in melanoma development. The aim of this study was to investigate the genetic variability of SPANX-B and SPANX-C in a sample of Sicilian male population including patients with melanoma of the skin and controls. A total of 99 patients were enrolled in this study. They included: 17 male patients with cutaneous melanoma and 82 normal males. Semiquantitative fluorescent multiplex PCR dosage analysis was carried out to identify the variety of classes of SPANX-B and SPANX-C genes. Sixteen and 13 genetic classes were detected for SPANX-B and SPANX-C genes, respectively. A statistical significant difference for a particular class of SPANX-C gene was found comparing patients with melanoma and controls (P=0.011). Further investigations should be conducted to confirm these observations and to evaluate the possible implication of other genes of the region Xq27-28 in melanoma.