Anti-Inflammatory Extract from Soil Algae Chromochloris zofingiensis Targeting TNFR/NF-κB Signaling at Different Levels.

Inflammatory skin diseases, including atopic dermatitis (AD) and psoriasis, are increasing in populations worldwide. The treatment of patients with AD and other forms of skin inflammation is mainly based on the use of topical corticosteroids or calcineurin inhibitors, which can cause significant side effects with long-term use. Therefore, there is a great need for the development of more effective and less toxic anti-inflammatory agents suitable for the treatment of chronic skin lesions. Here, we screened a number of strains from the ASIB 505 terrestrial algae collection and identified a green algae Chromochloris zofingiensis with pronounced anti-inflammatory properties. We found that a crude nonpolar extract of C. zofingiensis (ID name NAE_2022C), grown upon nitrogen deprivation, acts as a bioactive substance by inhibiting TNFR/NF-κB responses in human skin keratinocyte HaCaT cells. We also found that NAE_2022C suppressed the secretion of pro-inflammatory cytokine tumor necrosis factor α (TNFα) and several Th1- and Th2-related chemokines in a reconstituted human epidermis. The TNFR/NF-κB pathway analysis showed multiple inhibitory effects at different levels and disclosed a direct targeting of IKKß by the extract. Bioassay-guided fractionation followed by high-resolution mass spectrometry detected diacylglyceryl-trimethylhomoserine (DGTS), Lyso-DGTS (LDGTS), 5-phenylvaleric acid, theophylline and oleamide as leading metabolites in the active fraction of NAE_2022C. Further analysis identified betaine lipid DGTS (32:0) as one of the active compounds responsible for the NAE_2022C-mediated NF-κB suppression. Overall, this study presents an approach for the isolation, screening, and identification of anti-inflammatory secondary metabolites produced by soil algae.

Comparative study of substituted poly(4-vinylbenzyl chloride/ethylene glycol dimethacrylate) sorbents for enrichment of selected pharmaceuticals and estrogens from aqueous samples.

This study reports the syntheses of four polymeric sorbents based on nucleophilic substitution of Poly(4-vinylbenzylchloride/ethylene glycol dimethacrylate). Polymerization was executed by a simple thermal initiated bulk polymerization procedure. Ground polymer particles were functionalized through reaction with the nucleophiles triethylamine, imidazole, piperidine and pyrrolidine. Mixed-mode phases were characterized by infrared spectroscopy, nitrogen sorption porosimetry and potentiometric titration for determination of chloride content. Furthermore, materials were tested and evaluated for enrichment of seven pharmaceutical and endocrine-disrupting compounds at low ng mL-1 levels. Results demonstrate that the imidazole modified sorbent led to high and constant recovery rates for nearly all tested compounds. Therefore, this polymer was further tested for applicability with two environmental samples. Spiked tap and river water showed similar results as in evaluation experiments. Moreover, the developed method was validated regarding linearity, repeatability, instrumental limits and stability of analytes according to international guidelines.