RESUMO
Using the method of dominant lethal mutations, we assessed the frequency of the death of Drosophila melanogaster embryos under combined exposure to ionizing γ-radiation and non-ionizing pulsed magnetic field at various doses and modes of exposure. Mutagenic effect of combined exposure is antagonistic in nature. The antagonism is more pronounced when the following mode of exposure was used: exposure to non-ionizing pulsed magnetic field for 5 h followed by exposure to γ-radiation at doses of 3, 10, and 60 Gy. In case of reverse sequence of exposures, the antagonistic effect was statistically significant after exposure to γ-radiation at doses of 3 and 10 Gy, whereas at a dose of 20 Gy, a synergistic interaction was noted.
Assuntos
Drosophila melanogaster , Raios gama , Animais , Drosophila melanogaster/efeitos da radiação , Drosophila melanogaster/genética , Raios gama/efeitos adversos , Radiação Eletromagnética , Relação Dose-Resposta à Radiação , Campos Eletromagnéticos/efeitos adversos , Embrião não Mamífero/efeitos da radiação , Radiação Ionizante , Mutação/efeitos da radiação , Mutagênese/efeitos da radiaçãoRESUMO
NOD SCID mice were humanized by transplanting human hematopoietic cells isolated from umbilical cord blood. A dose-dependent death of hematopoietic cells and their subsequent recovery were shown after acute external γ-irradiation in the model of humanized mice. The proposed approach can be used for preclinical studies of radioprotective agents and for assessment of the impact of adverse factors on the survival rate and functional properties of human hematopoietic stem cells in vivo.
Assuntos
Transplante de Células-Tronco Hematopoéticas , Humanos , Animais , Camundongos , Camundongos SCID , Células-Tronco Hematopoéticas , Camundongos Endogâmicos NOD , Irradiação Corporal Total , Sangue Fetal , Transplante Heterólogo , Antígenos CD34RESUMO
The frequency of D. melanogaster embryonic death was estimated using the method of dominant lethal mutations after exposure to ionizing γ-radiation and non-ionizing pulsed magnetic field. γ-Radiation had a dose-dependent mutational effect on D. melanogaster. A pronounced increase in embryonic death was observed starting from a dose of 3 Gy and reaches a plateau at 60 Gy due to the maximum death of eggs. When D. melanogaster was exposed to pulsed magnetic field, the effect did not depend on the exposure time; a statistically significant genotoxic effect was detected after 5-h exposure.