Assuntos
Constipação Intestinal/etiologia , Síndrome de Fanconi/diagnóstico , Vômito/etiologia , Redução de Peso , Calcitriol/uso terapêutico , Ácido Cítrico/uso terapêutico , Constipação Intestinal/tratamento farmacológico , Cisteamina/uso terapêutico , Cistinose/diagnóstico , Cistinose/tratamento farmacológico , Cistinose/etiologia , Diagnóstico Diferencial , Síndrome de Fanconi/complicações , Síndrome de Fanconi/tratamento farmacológico , Humanos , Lactente , Masculino , Fósforo/uso terapêutico , Potássio/uso terapêutico , Sódio/uso terapêutico , Citrato de Sódio/uso terapêutico , Vômito/tratamento farmacológicoRESUMO
In this study, amorphous carbonaceous nanoparticles were prepared by a simple hydrothermal process using glucose as precursor. The nearly perfect spherical particles (beads) with the dimensions in the range of 10-500 nm were obtained depending on the main process parameters (precursor concentration, temperature, and time). The particles size, surface morphology, structure, and composition have been examined by TEM, SEM, X-ray diffraction, XPS, FTIR and Raman spectroscopy. These amorphous carbonaceous nanobeads (a-CNBs) have been found nontoxic in vitro with a variety of cultured cell lines. The size-dependent effect of a-CNBs addition on cell function has been observed. For example, a-CNBs can, in some cases, substantially increase interleukin-12 (IL-12) production by bone marrow dendritic cells. It has been further demonstrated that a-CNBs can be modified with fluorescent dye molecules or loaded with anti-cancer drugs for bioimaging or therapeutic purposes, respectively. The results of these tests and the strategies for the particle preparation and functionalization for biomedical applications have been discussed.