Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 3 de 3
Filtrar
Mais filtros











Base de dados
Intervalo de ano de publicação
1.
Res Vet Sci ; 159: 57-65, 2023 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-37084523

RESUMO

This study evaluated the influence of a temporary nutritional protein restriction (NPR) performed, under commercial conditions, in prepubertal female lambs on first lactation milk production traits and the inflammatory response triggered by an inflammatory challenge of the. From 40 Assaf female lambs, we defined a control group (Cn = 20), which received a standard diet for replacement lambs and the NPR group (n = 20), which received the same diet but without soybean meal between 3 and 5 months of age. About 150 days after lambing, 24 of these ewes (13 NPR, 11C) were subjected to an intramammary infusion of E. coli lipopolysaccharide (LPS). Our dynamic study identified indicator traits of local (SCC) and systemic (rectal Ta, IL-6, CXCL8, IL-10, IL-36RA, VEGF-A) response to the LPS challenge. The NPR did not show significant effects on milk production traits and did not affect the SCC and rectal Ta after the LPS challenge. However, the NPR had a significant influence on 8 of the 14 plasma biomarkers analysed, in all the cases with higher relative values in the C group. The effects observed on VEGF-A (involved in vasculogenesis during mammary gland development and vascular permeability) and IL-10 (a regulatory cytokine classically known by its anti-inflammatory action) are the most remarkable to explain the differences found between groups. Whereas further studies should be undertaken to confirm these results, our findings are of interest considering the current concern about the future world's demand for protein and the need for animal production systems to evolve toward sustainability.


Assuntos
Interleucina-10 , Leite , Animais , Ovinos , Feminino , Leite/metabolismo , Interleucina-10/metabolismo , Lipopolissacarídeos/farmacologia , Lipopolissacarídeos/metabolismo , Escherichia coli , Fator A de Crescimento do Endotélio Vascular/metabolismo , Lactação/fisiologia , Carneiro Doméstico , Proteínas Alimentares/metabolismo
2.
Genes (Basel) ; 11(5)2020 05 12.
Artigo em Inglês | MEDLINE | ID: mdl-32408659

RESUMO

Improved reproductive efficiency could lead to economic benefits for the beef industry, once the intensive selection pressure has led to a decreased fertility. However, several factors limit our understanding of fertility traits, including genetic differences between populations and statistical limitations. In the present study, the RNA-sequencing data from uterine samples of high-fertile (HF) and sub-fertile (SF) animals was integrated using co-expression network meta-analysis, weighted gene correlation network analysis, identification of upstream regulators, variant calling, and network topology approaches. Using this pipeline, top hub-genes harboring fixed variants (HF × SF) were identified in differentially co-expressed gene modules (DcoExp). The functional prioritization analysis identified the genes with highest potential to be key-regulators of the DcoExp modules between HF and SF animals. Consequently, 32 functional candidate genes (10 upstream regulators and 22 top hub-genes of DcoExp modules) were identified. These genes were associated with the regulation of relevant biological processes for fertility, such as embryonic development, germ cell proliferation, and ovarian hormone regulation. Additionally, 100 candidate variants (single nucleotide polymorphisms (SNPs) and insertions and deletions (INDELs)) were identified within those genes. In the long-term, the results obtained here may help to reduce the frequency of subfertility in beef herds, reducing the associated economic losses caused by this condition.


Assuntos
Doenças dos Bovinos/genética , Bovinos/genética , Fertilidade/genética , Redes Reguladoras de Genes , Estudos de Associação Genética/métodos , Infertilidade Feminina/veterinária , Metanálise em Rede , Animais , Conjuntos de Dados como Assunto , Endométrio/química , Estro , Feminino , Regulação da Expressão Gênica , Masculino , Gravidez , RNA Mensageiro/genética , RNA Mensageiro/isolamento & purificação , RNA-Seq
3.
Sci Rep ; 7: 45905, 2017 04 05.
Artigo em Inglês | MEDLINE | ID: mdl-28378756

RESUMO

In this study, RNA sequencing was used to obtain a comprehensive profile of the transcriptomic changes occurring in the mammary gland of lactating sheep suffering from fish oil-induced milk fat depression (FO-MFD). The milk somatic cell transcriptome analysis of four control and four FO-MFD ewes generated an average of 42 million paired-end reads per sample. In both conditions, less than 220 genes constitute approximately 89% of the total counts. These genes, which are considered as core genes, were mainly involved in cytoplasmic ribosomal proteins and electron transport chain pathways. In total, 117 genes were upregulated, and 96 genes were downregulated in FO-MFD samples. Functional analysis of the latter indicated a downregulation of genes involved in the SREBP signaling pathway (e.g., ACACA, ACSL, and ACSS) and Gene Ontology terms related to lipid metabolism and lipid biosynthetic processes. Integrated interpretation of upregulated genes indicated enrichment in genes encoding plasma membrane proteins and proteins regulating protein kinase activity. Overall, our results indicate that FO-MFD is associated with the downregulation of key genes involved in the mammary lipogenesis process. In addition, the results also suggest that this syndrome may be related to upregulation of other genes implicated in signal transduction and codification of transcription factors.


Assuntos
Depressão/genética , Metabolismo dos Lipídeos/genética , Leite/metabolismo , Ovinos/genética , Transcriptoma/genética , Ração Animal , Animais , Depressão/induzido quimicamente , Suplementos Nutricionais/efeitos adversos , Feminino , Óleos de Peixe/efeitos adversos , Perfilação da Expressão Gênica , Humanos , Lactação/genética , Lactação/metabolismo , Lipogênese/efeitos dos fármacos , Lipogênese/genética , Glândulas Mamárias Humanas/metabolismo , Glândulas Mamárias Humanas/patologia , Análise de Sequência de RNA , Ovinos/metabolismo
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA