RESUMO
During the 1918 influenza pandemic, children experienced substantially lower mortality than adults, a striking but unexplained finding. Whether this was due to enhanced resistance (reduced virus load) or better tolerance (reduced impact of infection) has not been defined. We found that prepubertal mice infected with H1N1 influenza virus also showed greater survival than infected pubertal mice, despite similar virus loads. Transcriptome profiling of infected lungs identified estrogen as a regulator of susceptibility in both sexes and also linked better survival to late expression of IL-1ß. Blocking puberty with gonadectomy or a gonadotropin-releasing hormone antagonist improved survival. Estrogen or testosterone (which can be converted to estrogen) restored susceptibility of gonadectomized pubertal mice to influenza mortality, but dihydrotestosterone (which cannot be converted to estrogen) did not. Estrogen receptor blockade with fulvestrant in both male and female pubertal mice resulted in improved survival, even when given 3 days after infection. Moreover, late, but not early, IL-1ß neutralization after infection was also protective. These findings indicate that pubertal increases in estrogen in both sexes are associated with increased mortality during influenza. This helps explain the reduced mortality of children seen with influenza in 1918 and might also be relevant to childhood tolerance to many other infectious diseases.
Assuntos
Vírus da Influenza A Subtipo H1N1/imunologia , Infecções por Orthomyxoviridae/imunologia , Maturidade Sexual , Adolescente , Animais , Criança , Pré-Escolar , Estradiol/análogos & derivados , Estradiol/farmacologia , Feminino , Fulvestranto , Humanos , Lactente , Recém-Nascido , Influenza Pandêmica, 1918-1919/mortalidade , Interleucina-1beta/genética , Interleucina-1beta/imunologia , Masculino , Camundongos , Infecções por Orthomyxoviridae/patologiaRESUMO
A second-degree epidermal scald burn in mice elicits an inflammatory response mediated by natural IgM directed to nonmuscle myosin with complement activation that results in ulceration and scarring. We find that such burn injury is associated with early mast cell (MC) degranulation and is absent in WBB6F1-Kit(W)/Kit(Wv) mice, which lack MCs in a context of other defects due to a mutation of the Kit receptor. To address further an MC role, we used transgenic strains with normal lineage development and a deficiency in a specific secretory granule component. Mouse strains lacking the MC-restricted chymase, mouse MC protease (mMCP)-4, or elastase, mMCP-5, show decreased injury after a second-degree scald burn, whereas mice lacking the MC-restricted tryptases, mMCP-6 and mMCP-7, or MC-specific carboxypeptidase A3 activity are not protected. Histologic sections showed some disruption of the epidermis at the scald site in the protected strains suggesting the possibility of topical reconstitution of full injury. Topical application of recombinant mMCP-5 or human neutrophil elastase to the scalded area increases epidermal injury with subsequent ulceration and scarring, both clinically and morphologically, in mMCP-5-deficient mice. Restoration of injury requires that topical administration of recombinant mMCP-5 occurs within the first hour postburn. Importantly, topical application of human MC chymase restores burn injury to scalded mMCP-4-deficient mice but not to mMCP-5-deficient mice revealing nonredundant actions for these two MC proteases in a model of innate inflammatory injury with remodeling.
Assuntos
Queimaduras/imunologia , Quimases/imunologia , Cicatriz/imunologia , Epiderme/imunologia , Mastócitos/imunologia , Modelos Imunológicos , Serina Endopeptidases/imunologia , Animais , Queimaduras/enzimologia , Queimaduras/genética , Queimaduras/patologia , Carboxipeptidases A/genética , Carboxipeptidases A/imunologia , Carboxipeptidases A/metabolismo , Degranulação Celular/genética , Degranulação Celular/imunologia , Quimases/genética , Quimases/metabolismo , Quimases/farmacologia , Cicatriz/enzimologia , Cicatriz/genética , Cicatriz/patologia , Epiderme/enzimologia , Epiderme/patologia , Humanos , Imunoglobulina M/genética , Imunoglobulina M/imunologia , Imunoglobulina M/metabolismo , Inflamação , Elastase de Leucócito/genética , Elastase de Leucócito/imunologia , Elastase de Leucócito/metabolismo , Elastase de Leucócito/farmacologia , Mastócitos/enzimologia , Mastócitos/patologia , Camundongos , Camundongos Endogâmicos BALB C , Camundongos Mutantes , Miosinas/genética , Miosinas/imunologia , Miosinas/metabolismo , Proteínas Proto-Oncogênicas c-kit , Serina Endopeptidases/genética , Serina Endopeptidases/metabolismo , Serina Endopeptidases/farmacologia , Triptases/genética , Triptases/imunologia , Triptases/metabolismo , Triptases/farmacologiaRESUMO
A major component of burn injury is caused by additional local damage from acute inflammation. Using a scald burn model in mice, we find that this part of the injury is dependent on recognition of self-antigen by specific natural IgM, leading to activation of the complement system. We propose that the depth of a burn wound is a sum of the thermal energy applied and of the degree of host inflammatory response.