RESUMO
A Gram-stain-negative, non-motile, ovoid or short rod shaped and aerobic marine bacterium, designated as strain LXJ103T, was isolated from a coastal phytoplankton bloom in Xiamen, PR China. Cells were oxidase- and catalase-positive. Strain LXJ103T grew at 4-40 °C (optimum, 28-37 °C), at pH 6-10 (optimum, pH 8.5) and with 1-15â% (w/v) NaCl (optimum, 3â%). The major cellular fatty acids (>10â%) were iso-C18â:â1 ω7c/iso-C18â:â1 ω6c (70.2â%) and C16â:â0 (10.3â%). The following polar lipids were found to be present: phosphatidylglycerol, phosphatidylethanolamine, two unidentified phospholipids and five unknown glycolipids. The predominant respiratory quinone was ubiquinone-10. Strain LXJ103T exhibited the highest 16S rRNA gene sequence similarity to Roseovarius litorisediminis D1-W8T (96.97â%). The phylogenetic trees based on 16S rRNA gene sequences showed that strain LXJ103T was a member of the genus Roseovarius. The draft genome size of strain LXJ103T is 3.05 Mb with a genomic G+C content of 61.22 mol%. The digital DNA-DNA genome hybridization value of strain LXJ103T compared with the most similar type strain R. litorisediminis CECT 8287T was 18.80â%. The average nucleotide identity value between strain LXJ103T and R. litorisediminis CECT 8287T was 72.60â%. On the basis of polyphasic data, strain LXJ103T represents a novel species of the genus Roseovarius, for which the name Roseovarius carneus sp. nov. is proposed. The type strain is LXJ103T (=CGMCC 1.19168T=MCCC 1K06527T=JCM 34778T).
Assuntos
Fosfatidiletanolaminas , Rhodobacteraceae , RNA Ribossômico 16S/genética , Catalase/genética , Filogenia , Ubiquinona/química , Fitoplâncton , Cloreto de Sódio , Composição de Bases , DNA Bacteriano/genética , Técnicas de Tipagem Bacteriana , Ácidos Graxos/química , Análise de Sequência de DNA , Fosfolipídeos/química , Glicolipídeos , Fosfatidilgliceróis , NucleotídeosRESUMO
A novel Gram-stain-negative, non-flagellated, non-motile, rod-shaped (0.4-0.6×1.8-2.5 µm), aerobic bacterial strain, designated JLT21T, was isolated from seawater of a shallow-sea hydrothermal system. Growth occurred with 0-4.0â% (w/v) NaCl (optimum, 2.0â% NaCl), at 8-45 °C (optimum, 25 °C) and at pH 3.0-10.0 (optimum, pH 7.0). Analysis of 16S rRNA gene sequences revealed that strain JLT21T showed the highest 16S rRNA gene sequence similarity to Gaetbulibacter aquiaggeris KEM-8T (97.2â%), Gaetbulibacter marinus IMCC1914T (96.9â%) and Yeosuana aromativorans GW1-1T (96.9â%). Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain JLT21T clustered with Y. aromativorans GW1-1T. The predominant respiratory quinone of strain JLT21T was menaquinone-6 (MK-6). Polar lipids were phosphatidylcholine, phosphatidylglycerol, phosphatidylethanolamine, three unidentified glycolipids and four unidentified lipids. The major fatty acids of strain JLT21T were iso-C15â:â0 (21.7â%), C16â:â1 ω6c/ω7c (11.5â%) and iso-C17â:â0 3-OH (10.9â%). The DNA G+C content of strain JLT21T was 32.6â%. On the basis of polyphasic analysis, strain JLT21T is considered to represent a novel species of the genus Yeosuana, for which the name Yeosuana marina sp. nov. is proposed. The type strain of Yeosuana marina is JLT21T (=CGMCC 1.15787T=JCM 31511T). The study helps us better understand the bacterial species in the shallow-sea hydrothermal system and their adaptations to the hydrothermal environment.