The use of morphological study technique for investigation of labial and palatine glands.

INTRODUCTION: Due to the deterioration of environmental conditions that promotes the onset of inflammatory and autoimmune diseases, and the progress in the diagnosis, the frequency of registration of intercurrent pathology of salivary glands has markedly risen in recent years, demonstrating the increased scientific interest in the research of the common and distinctive features of their structure. THE AIM: The paper was aimed at the development of the method of morphological study of human minor salivary (labial and palatine) glands by the use of plastic wax reconstruction to obtain the plastic model of the acini and ducts of human minor salivary glands. MATERIALS AND METHODS: Specimens of the glandular area of the hard palate mucosa and labial mucosa in its middle third have been studied. To gain the objective of the investigation the technique for morphological study of the human minor salivary (labial and palatine) glands is to be developed, encompassing the analysis of the spatial organization of the glandular epithelium of the labial and palatine glands together with blood microcirculatory flow by fixing the obtained specimens of the minor salivary glands in 4% glutaraldehyde solution and osmium tetroxide with subsequent embedding into the Epon-812, staining the serial semi-thin sections with phosphate buffered 0,1% toluidine blue solution, photomacrography of the distinguished boundaries of the investigated structures and obtaining of photoreconstructions. RESULTS AND CONCLUSIONS: Thus, the use of suggested technique enables to obtain the megascopic reconstruction of the acini and ducts of the labial and palatine glands, which can be studied from different sides, getting the full visualization of the shape and size, as well as to explore the glands' inner configuration, the geometry of the lumen of the epithelial excretory ducts, to determine changes in the thickness of the wall, to get a visual representation of the microtopographic interactions between the different parts of blood microcirculatory flow and excretory ducts of the minor salivary glands.

Report on the study of spatial organization of the human prostate glands.

OBJECTIVE: Introduction: In terms of the spatial organization human prostate is the complex organ due to the fact that it consists of several types of glands, localized in several histotopographic areas, characterized by the heterogeneous structure. On the other hand, most of the prostate glands are characterized by the high degree of adjacency to each other and quite complicated architectonics of both the external and internal contours of their acini and terminal ducts. The aim: The paper was aimed at the study of steromorphological features of the tubuloalveolar secretory elements in the peripheral area of the human prostate. PATIENTS AND METHODS: Material and Methods: 10 isolated postmortem specimens of the prostate gland, urinary bladder, seminal vesicles, fragments of the seminal ducts and urinary tracts which were taken from adult patients, died for the reasons not associated with the pathology of the urogenital system, have been analyzed to study the features of spatial organization of the human prostate glands in its peripheral area. To analyze the secretory components of the prostate stereological and decomposition methods have been used, which allow visualization of its structural and functional elements in all three inter-perpendicular planes. RESULTS: Results and Conclusions: The use of the suggested method enables to get the megascopic reconstruction of the acini and terminal ducts of the prostate gland which can be studied from all sides, getting a comprehensive idea about the shape and size, as well as allows to explore the inner topography of the organ's structure, the geometry of the lumen of the epithelial excretory ducts, to determine the changes in the thickness of the wall, to get a visual representation of microtopographic correlation between the different parts of blood microcirculatory flow with the acini and terminal ducts of the prostate gland.