[Action of red polarized light on the acupuncture point E-36 increases analgesic effect of corvitin].

In experiments on mice of lines C57BL/6J and CBA/CaLac, the possibility of strengthening of analgesic effect of corvitin by the action of red polarized light (PL) on the acupoint (AP) E-36 was studied. The pain behavioral response (licking of the painful area) was caused by injection of 5% formalin in hind limb (0.25 microl subcutaneously). The duration of pain response was studied before and after systemic introduction of corvitin (10 mg/kg, intraperitoneal) or joint use of corvitin and red PL (10 minute session). It is established, that after application of red PL on the antinociceptive AP E-36 in all animals an authentic strengthening of antinociceptive effect of corvitin takes place. In C57BL/6J mice, application of corvitin alone weakened the pain response by 29.7% and during combined use of red PL and corvitin, it grew up to 53.1%. Mice of line CBA/CaLac were less sensitive both to corvitin, and PL. In this line, corvitin used alone reduced the duration of pain response by 14%, and by 32.4% during combined use with red PL. Non-traumatic, without side effects, the method of influence by low-intensive PL can be recommended to patients accepting corvitin for strengthening its efficiency.

Antinociceptive effects of color polarized light in animal with formalin test.

Our recent results show the efficacy of pain suppression by exposure of antinociceptive acupuncture points (APs) to white polarized (P) light. But it is known that white light contains electromagnetic waves of different length (colors) and, possibly, not all of them produce a similar effect. There are no comparative data about analgesic affects of the different colors of P light now. The purpose of this study was to clear up a question if analgesic effects of low-intensive P light depend on the color of light/wavelength. Formalin-induced pain behavior (licking of the painful area) was tested in control mice and mice exposed to one of the color of P light (red, orange, yellow, green, blue, violet) on the painful area or AP E-36. Exposure of the painful area or AP E-36 to color P light evoked a statistically significant decrease of the licking time in mice to 31.5-64.1% and 36.1-54.4% respectively. The red light was the most effective for pain behavior depression, analgesia averaged 64.1% and 54.4% accordingly. The analgesic effects of red light in compare to three "cold" colors (blue, green, and violet) and white light was more pronounced in case of its application on the painful area than on AP E-36. In conclusion, the intensity of analgesic effects of P lights strongly depends on its color (wavelength).

[Suppression of visceral pain by action of the low intensity polarized light on acupuncture antinociceptive points]. / Pryhnichennia vistseral'noho boliu diieiu nyz'kointensyvnoho poliaryzovanoho svitla na protybol'ovi tochky akupunktury.

In experiments on mice, statistically authentic weakening of visceral pain has been shown after an action of low intensity polarized light from a device Bioptron on antinociceptive acupuncture points (AP). Pain was caused by an intraperitoneal injection of 2% acetic acid (0.1 ml/10 g). The intensity of pain was judged on duration and frequency of painful behavioral reactions (writhing, licking of abdomen), as well as on duration of sleep, eating and motor activity. In animals which immediately after injections of acetic acid were exposed to polarized light of low intensity for 10 min, applied on any of antinociceptive APs (E-36, E-43, VC-8, RP-6), the duration of painful behavioral reaction was determined to be reduced, while that of non-painful one increased. The comparison of the total duration of the writhing at control and experimental mice showed that an activation of AP E-43 induced the greatest analgesic effect (76.5%), from AP VC-8 it was 76.3%, from RP-6--46.8%, and from E-36--41.4%. We have concluded that the effect of polarized light of low intensity on APs was a convenient non-pharmacological method of treating visceral pain.

[The examination of the analgetic action of polarized light]. / Doslidzhennia anal'hetychnoï diï poliaryzovanoho svitla.

The influence of lowintensive polarized light on pain and nonepain behavioural responses (BR) was studied in experiments on mice with the locus of tonic pain (phormalin test). It was shown, that the action of polarized light on the acupuncture points (AP) statistically reliable decrease of pain BR (lisking of the locus of a pain), but increase the duration of nonepain BR (sleeping, eating). The analgetic effect depends from a choice of AP and ehpozition. The maximal analgesia (50%) was observed after 10-minute action of polarized light on AP E-36.